(Circular) (Six-base) MAPSORT of: pFN21AE1197.seq Check: 8865 from: 1 to: 7456 pFN21AE1197 7456 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5266 278 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2034 3032 4799 1937 Size: 97 998 1767 4594 Fragments arranged by size: 4594 1767 998 97 Acc65I G'GTAC_C Cuts at: 3504 4777 3504 Size: 1273 6183 AclI AA'CG_TT Cuts at: 1563 5583 5956 1563 Size: 4020 373 3063 Fragments arranged by size: 4020 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2570 2934 3780 3911 4420 5519 6903 7171 Size: 938 364 846 131 509 1099 1384 268 Cuts at: 7171 1632 Size: 1917 Fragments arranged by size: 1917 1384 1099 938 846 509 364 268 131 AfeI AGC'GCT Cuts at: 2995 2995 Size: 7456 AflII C'TTAA_G Cuts at: 820 1017 2524 820 Size: 197 1507 5752 Fragments arranged by size: 5752 1507 197 AflIII A'CryG_T Cuts at: 1203 3722 4138 4183 6355 7332 1203 Size: 2519 416 45 2172 977 1327 Fragments arranged by size: 2519 2172 1327 977 416 45 AgeI A'CCGG_T Cuts at: 6287 6287 Size: 7456 AhdI GACnn_n'nnGTC Cuts at: 6185 6185 Size: 7456 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7456 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2453 4620 1184 Size: 1269 2167 4020 Fragments arranged by size: 4020 2167 1269 AlwNI CAG_nnn'CTG Cuts at: 2080 2408 2449 3198 3463 3973 5199 6771 2080 Size: 328 41 749 265 510 1226 1572 2765 Fragments arranged by size: 2765 1572 1226 749 510 328 265 41 ApaI G_GGCC'C Cuts at: 2120 2510 3572 2120 Size: 390 1062 6004 Fragments arranged by size: 6004 1062 390 ApaLI G'TGCA_C Cuts at: 5513 6669 5513 Size: 1156 6300 ApoI r'AATT_y Cuts at: 1493 3812 4406 4472 4765 4991 5143 7252 1493 Size: 2319 594 66 293 226 152 2109 1697 Fragments arranged by size: 2319 2109 1697 594 293 226 152 66 AseI AT'TA_AT Cuts at: 160 1032 6010 160 Size: 872 4978 1606 Fragments arranged by size: 4978 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 AvaI C'yCGr_G Cuts at: 1870 1943 2113 2384 2647 4781 1870 Size: 73 170 271 263 2134 4545 Fragments arranged by size: 4545 2134 271 263 170 73 AvrII C'CTAG_G Cuts at: 2977 2977 Size: 7456 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3758 3791 3758 Size: 33 7423 BamHI G'GATC_C Cuts at: 4786 5153 4786 Size: 367 7089 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2124 2156 3504 4362 4777 Size: 325 217 627 337 32 1348 858 415 Cuts at: 4777 6232 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1348 858 627 415 337 325 217 32 BanII G_rGCy'C Cuts at: 1391 2120 2510 2656 3011 3572 3718 3883 4230 Size: 729 390 146 355 561 146 165 347 Cuts at: 4230 4775 1391 Size: 545 4072 Fragments arranged by size: 4072 729 561 545 390 355 347 165 146 146 BbeI G_GCGC'C Cuts at: 2160 2160 Size: 7456 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3674 3733 3836 928 Size: 971 1775 59 103 4548 Fragments arranged by size: 4548 1775 971 103 59 BbvCI CC'TCA_GC Cuts at: 3286 3286 Size: 7456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3056 3090 4070 4104 4597 4631 5647 Size: 34 1119 34 980 34 493 34 1016 Cuts at: 5647 5681 1903 Size: 34 3678 Fragments arranged by size: 3678 1119 1016 980 493 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2620 5348 6558 2620 Size: 2728 1210 3518 Fragments arranged by size: 3518 2728 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2319 2420 2651 Size: 107 122 71 631 605 647 101 231 Cuts at: 2651 2864 3203 3249 6067 136 Size: 213 339 46 2818 1525 Fragments arranged by size: 2818 1525 647 631 605 339 231 213 122 107 101 71 46 BglII A'GATC_T Cuts at: 7451 7451 Size: 7456 BlpI GC'TnA_GC Cuts at: 4873 4873 Size: 7456 Bme1580I G_kGCm'C Cuts at: 1790 2120 2510 2690 2860 3534 3572 4710 5517 Size: 330 390 180 170 674 38 1138 807 Cuts at: 5517 6673 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1138 807 674 390 330 180 170 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7456 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3534 4127 5187 Size: 416 53 83 399 24 2117 593 1060 Cuts at: 5187 6145 442 Size: 958 1753 Fragments arranged by size: 2117 1753 1060 958 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7456 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2491 4711 2491 Size: 2220 5236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2393 2430 3191 4258 6116 1239 Size: 1154 37 761 1067 1858 2579 Fragments arranged by size: 2579 1858 1154 1067 761 37 Bpu10I CC'TnA_GC Cuts at: 2558 3286 2558 Size: 728 6728 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4356 4540 5581 6446 6744 4356 Size: 184 1041 865 298 5068 Fragments arranged by size: 5068 1041 865 298 184 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6119 882 Size: 617 4620 2219 Fragments arranged by size: 4620 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4141 4186 5247 493 Size: 711 2937 45 1061 2702 Fragments arranged by size: 2937 2702 1061 711 45 BsaBI GATnn'nnATC Cuts at: 7450 7450 Size: 7456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 5263 5645 6280 Size: 53 83 186 936 624 3106 382 635 Cuts at: 6280 275 Size: 1451 Fragments arranged by size: 3106 1451 936 635 624 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2461 3053 3590 4672 5889 6287 6561 6708 7119 Size: 592 537 1082 1217 398 274 147 411 Cuts at: 7119 2461 Size: 2798 Fragments arranged by size: 2798 1217 1082 592 537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2403 2433 1178 Size: 30 165 30 1000 30 6201 Fragments arranged by size: 6201 1000 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2079 3971 1186 Size: 893 1892 4671 Fragments arranged by size: 4671 1892 893 BseYI C'CCAG_C Cuts at: 2023 2503 6659 7155 2023 Size: 480 4156 496 2324 Fragments arranged by size: 4156 2324 496 480 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 3279 4616 1163 Size: 573 297 1246 1337 4003 Fragments arranged by size: 4003 1337 1246 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2045 4516 4906 5667 6695 1540 Size: 275 186 44 2471 390 761 1028 2301 Fragments arranged by size: 2471 2301 1028 761 390 275 186 44 BsiHKAI G_wGCw'C Cuts at: 2912 3011 3061 3718 5517 5602 6673 2912 Size: 99 50 657 1799 85 1071 3695 Fragments arranged by size: 3695 1799 1071 657 99 85 50 BsmI GAATG_Cn' Cuts at: 2876 4967 5060 2876 Size: 2091 93 5272 Fragments arranged by size: 5272 2091 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2510 2656 2690 2860 2912 3011 Size: 399 330 390 146 34 170 52 99 Cuts at: 3011 3061 3534 3572 3718 3883 4230 4710 4775 Size: 50 473 38 146 165 347 480 65 Cuts at: 4775 5517 5602 6673 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 473 399 390 347 330 170 165 146 146 99 85 65 52 50 38 34 BspEI T'CCGG_A Cuts at: 2461 3053 3590 4672 7119 2461 Size: 592 537 1082 2447 2798 Fragments arranged by size: 2798 2447 1082 592 537 BspHI T'CATG_A Cuts at: 5345 7075 5345 Size: 1730 5726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3680 4644 4811 844 Size: 2836 964 167 3489 Fragments arranged by size: 3489 2836 964 167 BsrBI CCG'CTC Cuts at: 1394 1823 5343 1394 Size: 429 3520 3507 Fragments arranged by size: 3520 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4521 5951 6125 65 Size: 1183 3273 1430 174 1396 Fragments arranged by size: 3273 1430 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6100 6287 1700 Size: 110 4290 187 2869 Fragments arranged by size: 4290 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4135 4338 96 Size: 4039 203 3214 Fragments arranged by size: 4039 3214 203 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7456 BssSI C'ACGA_G Cuts at: 5516 6528 5516 Size: 1012 6444 BstAPI GCAn_nnn'nTGC Cuts at: 794 2449 794 Size: 1655 5801 BstBI TT'CG_AA Cuts at: 4489 6260 4489 Size: 1771 5685 BstEII G'GTnAC_C Cuts at: 1184 3279 1184 Size: 2095 5361 BstXI CCAn_nnnn'nTGG Cuts at: 2749 3344 4585 2749 Size: 595 1241 5620 Fragments arranged by size: 5620 1241 595 BstYI r'GATC_y Cuts at: 1972 4713 4786 5153 5538 5555 7007 7093 7105 Size: 2741 73 367 385 17 1452 86 12 Cuts at: 7105 7451 1972 Size: 346 1977 Fragments arranged by size: 2741 1977 1452 385 367 346 86 73 17 12 Bsu36I CC'TnA_GG Cuts at: 3456 3456 Size: 7456 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 2742 4242 513 Size: 552 939 414 324 1500 3727 Fragments arranged by size: 3727 1500 939 552 414 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6717 Fragments arranged by size: 6717 683 56 BtsI GCAGTG_nn' Cuts at: 2736 3224 4424 4972 5766 5786 2736 Size: 488 1200 548 794 20 4406 Fragments arranged by size: 4406 1200 794 548 488 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7456 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7421 DraI TTT'AAA Cuts at: 4760 4760 Size: 7456 DraIII CAC_nnn'GTG Cuts at: 4657 4657 Size: 7456 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6463 809 Size: 507 5147 1802 Fragments arranged by size: 5147 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2042 2421 2865 Size: 54 1425 50 275 18 212 379 444 Cuts at: 2865 3117 4903 5792 7421 8 Size: 252 1786 889 1629 43 Fragments arranged by size: 1786 1629 1425 889 444 379 275 252 212 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2042 4903 1537 Size: 275 230 2861 4090 Fragments arranged by size: 4090 2861 275 230 EarI CTCTTCn'nnn_ Cuts at: 1353 3323 4439 5386 1353 Size: 1970 1116 947 3423 Fragments arranged by size: 3423 1970 1116 947 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3126 6043 6417 6563 387 Size: 1064 138 1537 2917 374 146 1280 Fragments arranged by size: 2917 1537 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3009 3716 3009 Size: 707 6749 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2393 2430 2570 2934 3191 3780 3911 Size: 393 761 37 140 364 257 589 131 Cuts at: 3911 4258 4420 5519 6116 6903 7171 1239 Size: 347 162 1099 597 787 268 1524 Fragments arranged by size: 1524 1099 787 761 597 589 393 364 347 268 257 162 140 131 37 EcoNI CCTnn'n_nnAGG Cuts at: 2336 2561 2336 Size: 225 7231 EcoO109I rG'GnC_Cy Cuts at: 2117 2312 2507 3232 3266 3569 2117 Size: 195 195 725 34 303 6004 Fragments arranged by size: 6004 725 303 195 195 34 EcoRI G'AATT_C Cuts at: 4406 4765 5143 4406 Size: 359 378 6719 Fragments arranged by size: 6719 378 359 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3760 3760 Size: 7456 FspI TGC'GCA Cuts at: 5962 5962 Size: 7456 HaeII r_GCGC'y Cuts at: 1678 2160 2997 3198 6603 1678 Size: 482 837 201 3405 2531 Fragments arranged by size: 3405 2531 837 482 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2382 2414 3572 3604 6097 Size: 32 135 32 810 32 1158 32 2493 Cuts at: 6097 6129 6171 6203 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2493 1158 810 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4270 4800 5046 669 Size: 988 281 2332 530 246 3079 Fragments arranged by size: 3079 2332 988 530 281 246 HindIII A'AGCT_T Cuts at: 748 3693 748 Size: 2945 4511 HpaI GTT'AAC Cuts at: 5046 5046 Size: 7456 KasI G'GCGC_C Cuts at: 2156 2156 Size: 7456 KpnI G_GTAC'C Cuts at: 3508 4781 3508 Size: 1273 6183 MfeI C'AATT_G Cuts at: 5055 5055 Size: 7456 MluI A'CGCG_T Cuts at: 7332 7332 Size: 7456 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2745 6570 6754 1305 Size: 1440 3825 184 2007 Fragments arranged by size: 3825 2007 1440 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5634 Fragments arranged by size: 5634 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4697 5414 5773 5932 518 Size: 648 843 2688 717 359 159 2042 Fragments arranged by size: 2688 2042 843 717 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2449 2480 2532 2540 2628 2837 3074 3181 Size: 843 31 52 8 88 209 237 107 Cuts at: 3181 3263 3758 4872 5549 6697 6942 1606 Size: 82 495 1114 677 1148 245 2120 Fragments arranged by size: 2120 1148 1114 843 677 495 245 237 209 107 88 82 52 31 8 NarI GG'CG_CC Cuts at: 2157 2157 Size: 7456 NcoI C'CATG_G Cuts at: 513 1065 2004 2742 4242 513 Size: 552 939 738 1500 3727 Fragments arranged by size: 3727 1500 939 738 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7456 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7456 NotI GC'GGCC_GC Cuts at: 4903 4903 Size: 7456 NruI TCG'CGA Cuts at: 3090 3090 Size: 7456 NspI r_CATG'y Cuts at: 1132 1229 2809 3610 3726 4324 4530 4814 6359 Size: 97 1580 801 116 598 206 284 1545 Cuts at: 6359 1132 Size: 2229 Fragments arranged by size: 2229 1580 1545 801 598 284 206 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7456 PciI A'CATG_T Cuts at: 3722 6355 3722 Size: 2633 4823 PflMI CCAn_nnn'nTGG Cuts at: 1108 2408 4248 4454 1108 Size: 1300 1840 206 4110 Fragments arranged by size: 4110 1840 1300 206 PfoI T'CCnGG_A Cuts at: 2331 3011 4026 4217 4274 4331 4584 2331 Size: 680 1015 191 57 57 253 5203 Fragments arranged by size: 5203 1015 680 253 191 57 57 PmeI GTTT'AAAC Cuts at: 4760 4760 Size: 7456 PmlI CAC'GTG Cuts at: 4141 4186 4141 Size: 45 7411 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5525 5557 7038 7070 5525 Size: 32 1481 32 5911 Fragments arranged by size: 5911 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3232 3266 3232 Size: 34 7422 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7456 PsiI TTA'TAA Cuts at: 5026 5026 Size: 7456 PspOMI G'GGCC_C Cuts at: 2116 2506 3568 2116 Size: 390 1062 6004 Fragments arranged by size: 6004 1062 390 PstI C_TGCA'G Cuts at: 830 2178 3001 4808 830 Size: 1348 823 1807 3478 Fragments arranged by size: 3478 1807 1348 823 PvuI CG_AT'CG Cuts at: 2001 4516 2001 Size: 2515 4941 PvuII CAG'CTG Cuts at: 2449 2480 2532 3074 2449 Size: 31 52 542 6831 Fragments arranged by size: 6831 542 52 31 RsrII CG'GwC_CG Cuts at: 3122 3122 Size: 7456 SacI G_AGCT'C Cuts at: 3011 3718 3011 Size: 707 6749 SalI G'TCGA_C Cuts at: 1936 4798 1936 Size: 2862 4594 SanDI GG'GwC_CC Cuts at: 3266 3266 Size: 7456 SbfI CC_TGCA'GG Cuts at: 4808 4808 Size: 7456 ScaI AGT'ACT Cuts at: 4214 5704 7416 4214 Size: 1490 1712 4254 Fragments arranged by size: 4254 1712 1490 SexAI A'CCwGG_T Cuts at: 2325 2325 Size: 7456 SfcI C'TryA_G Cuts at: 826 1045 2174 2997 3426 3914 4804 5939 6620 Size: 219 1129 823 429 488 890 1135 681 Cuts at: 6620 6811 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1129 890 823 681 488 429 219 191 SfoI GGC'GCC Cuts at: 2158 2158 Size: 7456 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7456 SmaI CCC'GGG Cuts at: 1872 2115 4783 1872 Size: 243 2668 4545 Fragments arranged by size: 4545 2668 243 SmlI C'TyrA_G Cuts at: 820 1017 1943 2524 4371 4555 5560 6461 6723 Size: 197 926 581 1847 184 1005 901 262 Cuts at: 6723 820 Size: 1553 Fragments arranged by size: 1847 1553 1005 926 901 581 262 197 184 SnaBI TAC'GTA Cuts at: 493 5247 493 Size: 4754 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7456 SphI G_CATG'C Cuts at: 1132 4324 4530 4814 1132 Size: 3192 206 284 3774 Fragments arranged by size: 3774 3192 284 206 SspI AAT'ATT Cuts at: 5 52 5380 5 Size: 47 5328 2081 Fragments arranged by size: 5328 2081 47 StuI AGG'CCT Cuts at: 1517 2645 4160 1517 Size: 1128 1515 4813 Fragments arranged by size: 4813 1515 1128 StyI C'CwwG_G Cuts at: 513 1065 2004 2667 2712 2742 2977 4155 4242 Size: 552 939 663 45 30 235 1178 87 Cuts at: 4242 4895 513 Size: 653 3074 Fragments arranged by size: 3074 1178 939 663 653 552 235 87 45 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2142 2741 3690 4766 5483 5500 5653 Size: 1255 562 599 949 1076 717 17 153 Cuts at: 5653 5838 6283 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1076 949 717 599 562 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4135 4212 4338 Size: 274 80 33 51 1443 2158 77 126 Cuts at: 4338 5702 7414 96 Size: 1364 1712 138 Fragments arranged by size: 2158 1712 1443 1364 274 138 126 80 77 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2403 2435 4397 4429 7416 7448 2403 Size: 32 1962 32 2987 32 2411 Fragments arranged by size: 2987 2411 1962 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3979 1316 Size: 2663 4793 XbaI T'CTAG_A Cuts at: 4792 4792 Size: 7456 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 2323 Size: 7456 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7456 XmaI C'CCGG_G Cuts at: 1870 2113 4781 1870 Size: 243 2668 4545 Fragments arranged by size: 4545 2668 243 XmnI GAAnn'nnTTC Cuts at: 2081 2790 5585 2081 Size: 709 2795 3952 Fragments arranged by size: 3952 2795 709 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5264 276 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PspXI PsrI SacII SapI SfiI SrfI SwaI