(Linear) (Six-base) MAPSORT of: KIEE1197.seq Check: 6523 from: 1 to: 2751 KIEE1197.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AccI GT'mk_AC Cuts at: 0 29 1027 2751 Size: 29 998 1724 Fragments arranged by size: 1724 998 29 Acc65I G'GTAC_C Cuts at: 0 1499 2751 Size: 1499 1252 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 929 1775 1906 2415 2751 Size: 565 364 846 131 509 336 Fragments arranged by size: 846 565 509 364 336 131 AfeI AGC'GCT Cuts at: 0 990 2751 Size: 990 1761 AflII C'TTAA_G Cuts at: 0 519 2751 Size: 519 2232 AflIII A'CryG_T Cuts at: 0 1717 2133 2178 2751 Size: 1717 416 45 573 Fragments arranged by size: 1717 573 416 45 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 448 2615 2751 Size: 448 2167 136 Fragments arranged by size: 2167 448 136 AlwNI CAG_nnn'CTG Cuts at: 0 75 403 444 1193 1458 1968 2751 Size: 75 328 41 749 265 510 783 Fragments arranged by size: 783 749 510 328 265 75 41 ApaI G_GGCC'C Cuts at: 0 115 505 1567 2751 Size: 115 390 1062 1184 Fragments arranged by size: 1184 1062 390 115 ApoI r'AATT_y Cuts at: 0 1807 2401 2467 2751 Size: 1807 594 66 284 Fragments arranged by size: 1807 594 284 66 AvaI C'yCGr_G Cuts at: 0 108 379 642 2751 Size: 108 271 263 2109 Fragments arranged by size: 2109 271 263 108 AvrII C'CTAG_G Cuts at: 0 972 2751 Size: 972 1779 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1753 1786 2751 Size: 1753 33 965 Fragments arranged by size: 1753 965 33 BanI G'GyrC_C Cuts at: 0 119 151 1499 2357 2751 Size: 119 32 1348 858 394 Fragments arranged by size: 1348 858 394 119 32 BanII G_rGCy'C Cuts at: 0 115 505 651 1006 1567 1713 1878 2225 Size: 115 390 146 355 561 146 165 347 Cuts at: 2225 2751 Size: 526 Fragments arranged by size: 561 526 390 355 347 165 146 146 115 BbeI G_GCGC'C Cuts at: 0 155 2751 Size: 155 2596 BbsI GAAGACnn'nnnn_ Cuts at: 0 1669 1728 1831 2751 Size: 1669 59 103 920 Fragments arranged by size: 1669 920 103 59 BbvCI CC'TCA_GC Cuts at: 0 1281 2751 Size: 1281 1470 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1051 1085 2065 2099 2592 2626 2751 Size: 1051 34 980 34 493 34 125 Fragments arranged by size: 1051 980 493 125 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 615 2751 Size: 615 2136 BglI GCCn_nnn'nGGC Cuts at: 0 314 415 646 859 1198 1244 2751 Size: 314 101 231 213 339 46 1507 Fragments arranged by size: 1507 339 314 231 213 101 46 Bme1580I G_kGCm'C Cuts at: 0 115 505 685 855 1529 1567 2705 2751 Size: 115 390 180 170 674 38 1138 46 Fragments arranged by size: 1138 674 390 180 170 115 46 38 BmrI ACTGGGnnnn_n' Cuts at: 0 1529 2122 2751 Size: 1529 593 629 Fragments arranged by size: 1529 629 593 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 486 2706 2751 Size: 486 2220 45 Fragments arranged by size: 2220 486 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 425 1186 2253 2751 Size: 388 37 761 1067 498 Fragments arranged by size: 1067 761 498 388 37 Bpu10I CC'TnA_GC Cuts at: 0 553 1281 2751 Size: 553 728 1470 Fragments arranged by size: 1470 728 553 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2351 2535 2751 Size: 2351 184 216 Fragments arranged by size: 2351 216 184 BsaAI yAC'GTr Cuts at: 0 2136 2181 2751 Size: 2136 45 570 Fragments arranged by size: 2136 570 45 BsaHI Gr'CG_yC Cuts at: 0 152 2751 Size: 152 2599 BsaWI w'CCGG_w Cuts at: 0 456 1048 1585 2667 2751 Size: 456 592 537 1082 84 Fragments arranged by size: 1082 592 537 456 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 398 428 2751 Size: 398 30 2323 Fragments arranged by size: 2323 398 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 74 1966 2751 Size: 74 1892 785 Fragments arranged by size: 1892 785 74 BseYI C'CCAG_C Cuts at: 0 18 498 2751 Size: 18 480 2253 Fragments arranged by size: 2253 480 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 1274 2611 2751 Size: 28 1246 1337 140 Fragments arranged by size: 1337 1246 140 28 BsiEI CG_ry'CG Cuts at: 0 40 2511 2751 Size: 40 2471 240 Fragments arranged by size: 2471 240 40 BsiHKAI G_wGCw'C Cuts at: 0 907 1006 1056 1713 2751 Size: 907 99 50 657 1038 Fragments arranged by size: 1038 907 657 99 50 BsmI GAATG_Cn' Cuts at: 0 871 2751 Size: 871 1880 Bsp1286I G_dGCh'C Cuts at: 0 115 505 651 685 855 907 1006 1056 Size: 115 390 146 34 170 52 99 50 Cuts at: 1056 1529 1567 1713 1878 2225 2705 2751 Size: 473 38 146 165 347 480 46 Fragments arranged by size: 480 473 390 347 170 165 146 146 115 99 52 50 46 38 34 BspEI T'CCGG_A Cuts at: 0 456 1048 1585 2667 2751 Size: 456 592 537 1082 84 Fragments arranged by size: 1082 592 537 456 84 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1675 2639 2751 Size: 1675 964 112 Fragments arranged by size: 1675 964 112 BsrDI GCAATG_nn' Cuts at: 0 2516 2751 Size: 2516 235 BsrGI T'GTAC_A Cuts at: 0 2130 2333 2751 Size: 2130 203 418 Fragments arranged by size: 2130 418 203 BstAPI GCAn_nnn'nTGC Cuts at: 0 444 2751 Size: 444 2307 BstBI TT'CG_AA Cuts at: 0 2484 2751 Size: 2484 267 BstEII G'GTnAC_C Cuts at: 0 1274 2751 Size: 1274 1477 BstXI CCAn_nnnn'nTGG Cuts at: 0 744 1339 2580 2751 Size: 744 595 1241 171 Fragments arranged by size: 1241 744 595 171 BstYI r'GATC_y Cuts at: 0 2708 2751 Size: 2708 43 Bsu36I CC'TnA_GG Cuts at: 0 1451 2751 Size: 1451 1300 BtgI C'CryG_G Cuts at: 0 413 737 2237 2751 Size: 413 324 1500 514 Fragments arranged by size: 1500 514 413 324 BtsI GCAGTG_nn' Cuts at: 0 731 1219 2419 2751 Size: 731 488 1200 332 Fragments arranged by size: 1200 731 488 332 DraIII CAC_nnn'GTG Cuts at: 0 2652 2751 Size: 2652 99 EaeI y'GGCC_r Cuts at: 0 37 416 860 1112 2751 Size: 37 379 444 252 1639 Fragments arranged by size: 1639 444 379 252 37 EagI C'GGCC_G Cuts at: 0 37 2751 Size: 37 2714 EarI CTCTTCn'nnn_ Cuts at: 0 1318 2434 2751 Size: 1318 1116 317 Fragments arranged by size: 1318 1116 317 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1121 2751 Size: 1121 1630 EcoICRI GAG'CTC Cuts at: 0 1004 1711 2751 Size: 1004 707 1040 Fragments arranged by size: 1040 1004 707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 425 565 929 1186 1775 1906 2253 Size: 388 37 140 364 257 589 131 347 Cuts at: 2253 2415 2751 Size: 162 336 Fragments arranged by size: 589 388 364 347 336 257 162 140 131 37 EcoNI CCTnn'n_nnAGG Cuts at: 0 331 556 2751 Size: 331 225 2195 Fragments arranged by size: 2195 331 225 EcoO109I rG'GnC_Cy Cuts at: 0 112 307 502 1227 1261 1564 2751 Size: 112 195 195 725 34 303 1187 Fragments arranged by size: 1187 725 303 195 195 112 34 EcoRI G'AATT_C Cuts at: 0 2401 2751 Size: 2401 350 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1755 2751 Size: 1755 996 HaeII r_GCGC'y Cuts at: 0 155 992 1193 2751 Size: 155 837 201 1558 Fragments arranged by size: 1558 837 201 155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 377 409 1567 1599 2751 Size: 377 32 1158 32 1152 Fragments arranged by size: 1158 1152 377 32 32 HincII GTy'rAC Cuts at: 0 2265 2751 Size: 2265 486 HindIII A'AGCT_T Cuts at: 0 1688 2751 Size: 1688 1063 KasI G'GCGC_C Cuts at: 0 151 2751 Size: 151 2600 KpnI G_GTAC'C Cuts at: 0 1503 2751 Size: 1503 1248 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 740 2751 Size: 740 2011 MslI CAynn'nnrTG Cuts at: 0 2692 2751 Size: 2692 59 MspA1I CmG'CkG Cuts at: 0 444 475 527 535 623 832 1069 1176 Size: 444 31 52 8 88 209 237 107 Cuts at: 1176 1258 1753 2751 Size: 82 495 998 Fragments arranged by size: 998 495 444 237 209 107 88 82 52 31 8 NarI GG'CG_CC Cuts at: 0 152 2751 Size: 152 2599 NcoI C'CATG_G Cuts at: 0 737 2237 2751 Size: 737 1500 514 Fragments arranged by size: 1500 737 514 NruI TCG'CGA Cuts at: 0 1085 2751 Size: 1085 1666 NspI r_CATG'y Cuts at: 0 804 1605 1721 2319 2525 2751 Size: 804 801 116 598 206 226 Fragments arranged by size: 804 801 598 226 206 116 PciI A'CATG_T Cuts at: 0 1717 2751 Size: 1717 1034 PflMI CCAn_nnn'nTGG Cuts at: 0 403 2243 2449 2751 Size: 403 1840 206 302 Fragments arranged by size: 1840 403 302 206 PfoI T'CCnGG_A Cuts at: 0 326 1006 2021 2212 2269 2326 2579 2751 Size: 326 680 1015 191 57 57 253 172 Fragments arranged by size: 1015 680 326 253 191 172 57 57 PmlI CAC'GTG Cuts at: 0 2136 2181 2751 Size: 2136 45 570 Fragments arranged by size: 2136 570 45 PpuMI rG'GwC_Cy Cuts at: 0 1227 1261 2751 Size: 1227 34 1490 Fragments arranged by size: 1490 1227 34 PspOMI G'GGCC_C Cuts at: 0 111 501 1563 2751 Size: 111 390 1062 1188 Fragments arranged by size: 1188 1062 390 111 PstI C_TGCA'G Cuts at: 0 173 996 2751 Size: 173 823 1755 Fragments arranged by size: 1755 823 173 PvuI CG_AT'CG Cuts at: 0 2511 2751 Size: 2511 240 PvuII CAG'CTG Cuts at: 0 444 475 527 1069 2751 Size: 444 31 52 542 1682 Fragments arranged by size: 1682 542 444 52 31 RsrII CG'GwC_CG Cuts at: 0 1117 2751 Size: 1117 1634 SacI G_AGCT'C Cuts at: 0 1006 1713 2751 Size: 1006 707 1038 Fragments arranged by size: 1038 1006 707 SanDI GG'GwC_CC Cuts at: 0 1261 2751 Size: 1261 1490 ScaI AGT'ACT Cuts at: 0 2209 2751 Size: 2209 542 SexAI A'CCwGG_T Cuts at: 0 320 2751 Size: 320 2431 SfcI C'TryA_G Cuts at: 0 169 992 1421 1909 2751 Size: 169 823 429 488 842 Fragments arranged by size: 842 823 488 429 169 SfoI GGC'GCC Cuts at: 0 153 2751 Size: 153 2598 SmaI CCC'GGG Cuts at: 0 110 2751 Size: 110 2641 SmlI C'TyrA_G Cuts at: 0 519 2366 2550 2751 Size: 519 1847 184 201 Fragments arranged by size: 1847 519 201 184 SphI G_CATG'C Cuts at: 0 2319 2525 2751 Size: 2319 206 226 Fragments arranged by size: 2319 226 206 StuI AGG'CCT Cuts at: 0 640 2155 2751 Size: 640 1515 596 Fragments arranged by size: 1515 640 596 StyI C'CwwG_G Cuts at: 0 662 707 737 972 2150 2237 2751 Size: 662 45 30 235 1178 87 514 Fragments arranged by size: 1178 662 514 235 87 45 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 137 736 1685 2751 Size: 137 599 949 1066 Fragments arranged by size: 1066 949 599 137 TatI w'GTAC_w Cuts at: 0 2130 2207 2333 2751 Size: 2130 77 126 418 Fragments arranged by size: 2130 418 126 77 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 398 430 2392 2424 2751 Size: 398 32 1962 32 327 Fragments arranged by size: 1962 398 327 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1974 2751 Size: 1974 777 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 318 2751 Size: 318 2433 XmaI C'CCGG_G Cuts at: 0 108 2751 Size: 108 2643 XmnI GAAnn'nnTTC Cuts at: 0 76 785 2751 Size: 76 709 1966 Fragments arranged by size: 1966 709 76 Enzymes that do cut: AccI Acc65I AcuI AfeI AflII AflIII AlfI AlwNI ApaI ApoI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MslI MspA1I NarI NcoI NruI NspI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SanDI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI BamHI BclI BfrBI BglII BlpI BmgBI BmtI BsaI BsaBI BsiWI BsmBI BspHI BsrBI BsrFI BssHII BssSI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRV FseI FspI FspAI HpaI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NsiI PacI PasI PmeI PpiI PshAI PsiI PspXI PsrI SacII SalI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI