################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AspI AvrII BbrPI BfrI BlnI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstEII BstPI BstZ17I CpoI DraI DraIII DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO65I FseI HincII HindII HindIII HpaI KspI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 1543 GGTACC G^GTACC AcsI 449 GAATTC [GA]^AATT[CT] AcyI 965 GGCGCC G[GA]^CG[CT]C AflIII 1472 ACGTGT A^C[GA][CT]GT ApoI 449 GAATTC [GA]^AATT[CT] Asp700I 457 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1543 GGTACC G^GTACC AspHI 1531 GTGCTC G[AT]GC[AT]^C AvaI 1739 CCCGGG C^[CT]CG[GA]G AviII 1568 TGCGCA TGC^GCA BamHI 351 GGATCC G^GATCC BbeI 965 GGCGCC GGCGC^C BbvCI 1733 CCTCAGC CCTCAGC(-5/-2) BcgI 1148 GCATTCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1680 GGATAC GTATCC(6/5) BsaBI 1425 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 965 GGCGCC G[GA]^CG[CT]C BsiHKAI 1531 GTGCTC G[AT]GC[AT]^C BsoBI 1739 CCCGGG C^[CT]CG[GA]G BspDI 631 ATCGAT AT^CGAT BsrBI 2428 CCGCTC CCGCTC(-3/-3) BssHII 40 GCGCGC G^CGCGC BstBI 833 TTCGAA TT^CGAA BstXI 2123 CCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 631 ATCGAT AT^CGAT DrdI 1624 GACTTCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 449 GAATTC G^AATTC EcoRV 958 GATATC GAT^ATC EcoT22I 1001 ATGCAT ATGCA^T FspI 1568 TGCGCA TGC^GCA Hin1I 965 GGCGCC G[GA]^CG[CT]C KasI 965 GGCGCC G^GCGCC KpnI 1543 GGTACC GGTAC^C MamI 1425 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2364 GCCGGC GCC^GGC NarI 965 GGCGCC GG^CGCC NgoMIV 2364 GCCGGC G^CCGGC NsiI 1001 ATGCAT ATGCA^T NspI 2300 GCATGC [GA]CATG^[CT] NspV 833 TTCGAA TT^CGAA SfiI 2367 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 965 GGCGCC GGC^GCC SfuI 833 TTCGAA TT^CGAA SmaI 1739 CCCGGG CCC^GGG SphI 2300 GCATGC GCATG^C Tsp509I 450 AATT ^AATT XbaI 1254 TCTAGA T^CTAGA XcmI 1643 CCATCCTGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1739 CCCGGG C^CCGGG XmaI 1739 CCCGGG C^CCGGG XmnI 457 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AhdI 1245 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2093 GACTCCTGGTC AspEI 1245 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2093 GACTCCTGGTC BalI 197 TGGCCA TGG^CCA 2525 TGGCCA BclI 1424 TGATCA T^GATCA 1649 TGATCA BglII 1370 AGATCT A^GATCT 1430 AGATCT BlpI 1171 GCTCAGC GC^T[ACGT]AGC 2202 GCTCAGC Bpu1102I 1171 GCTCAGC GC^T[ACGT]AGC 2202 GCTCAGC BsaI 178 GAGACC GGTCTC(1/5) 1243 GAGACC BsmI 1003 GCATTC GAATGC(1/-1) 1148 GCATTC BspMI 170 ACCTGC ACCTGC(4/8) 1919 ACCTGC BstAPI 519 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 792 GCAGGCACTGC CelII 1171 GCTCAGC GC^T[ACGT]AGC 2202 GCTCAGC Eam1105I 1245 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2093 GACTCCTGGTC EcoT14I 149 CCAAGG C^C[AT][AT]GG 2477 CCAAGG FbaI 1424 TGATCA T^GATCA 1649 TGATCA HaeII 839 GGCGCT [GA]GCGC^[CT] 965 GGCGCC HgaI 1608 GCGTC GACGC(5/10) 2417 GCGTC MluNI 197 TGGCCA TGG^CCA 2525 TGGCCA MscI 197 TGGCCA TGG^CCA 2525 TGGCCA MseI 281 TTAA T^TAA 895 TTAA PflMI 1014 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1755 CCACCCACTGG SapI 399 GCTCTTC GCTCTTC(1/4) 936 GCTCTTC StyI 149 CCAAGG C^C[AT][AT]GG 2477 CCAAGG TfiI 462 GATTC G^A[AT]TC 472 GATTC Tru9I 281 TTAA T^TAA 895 TTAA Van91I 1014 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1755 CCACCCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XhoII