(Circular) (Six-base) MAPSORT of: pFN21AE0697.seq Check: 3203 from: 1 to: 7243 pFN21AE0697 7243 bp With 182 enzymes: SgfI * March 2, 2010 13:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5053 278 Size: 53 83 186 936 3517 2468 Fragments arranged by size: 3517 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4586 1937 Size: 2649 4594 Acc65I G'GTAC_C Cuts at: 3548 4564 3548 Size: 1016 6227 AclI AA'CG_TT Cuts at: 1563 5370 5743 1563 Size: 3807 373 3063 Fragments arranged by size: 3807 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4426 4442 5306 6690 6958 1632 Size: 2794 16 864 1384 268 1917 Fragments arranged by size: 2794 1917 1384 864 268 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7046 AflIII A'CryG_T Cuts at: 1203 3477 6142 7119 1203 Size: 2274 2665 977 1327 Fragments arranged by size: 2665 2274 1327 977 AgeI A'CCGG_T Cuts at: 6074 6074 Size: 7243 AhdI GACnn_n'nnGTC Cuts at: 3255 4103 5972 3255 Size: 848 1869 4526 Fragments arranged by size: 4526 1869 848 AleI CACnn'nnGTG Cuts at: 1166 2492 3143 3992 1166 Size: 1326 651 849 4417 Fragments arranged by size: 4417 1326 849 651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2783 1184 Size: 1599 5644 AlwNI CAG_nnn'CTG Cuts at: 2026 2371 2803 3025 3184 4055 4465 4986 6558 Size: 345 432 222 159 871 410 521 1572 Cuts at: 6558 2026 Size: 2711 Fragments arranged by size: 2711 1572 871 521 432 410 345 222 159 ApaI G_GGCC'C Cuts at: 2278 2912 3506 2278 Size: 634 594 6015 Fragments arranged by size: 6015 634 594 ApaLI G'TGCA_C Cuts at: 5300 6456 5300 Size: 1156 6087 ApoI r'AATT_y Cuts at: 1493 2454 4552 4778 4930 7039 1493 Size: 961 2098 226 152 2109 1697 Fragments arranged by size: 2109 2098 1697 961 226 152 AseI AT'TA_AT Cuts at: 160 1032 5797 160 Size: 872 4765 1606 Fragments arranged by size: 4765 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7243 AvaI C'yCGr_G Cuts at: 1870 1943 3744 4568 1870 Size: 73 1801 824 4545 Fragments arranged by size: 4545 1801 824 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4244 4277 4244 Size: 33 7210 BamHI G'GATC_C Cuts at: 2356 4573 4940 2356 Size: 2217 367 4659 Fragments arranged by size: 4659 2217 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2587 2815 2970 3487 3548 Size: 325 217 627 800 228 155 517 61 Cuts at: 3548 3709 4564 6019 618 Size: 161 855 1455 1842 Fragments arranged by size: 1842 1455 855 800 627 517 325 228 217 161 155 61 BanII G_rGCy'C Cuts at: 1391 2278 2912 3178 3506 3908 4143 4562 1391 Size: 887 634 266 328 402 235 419 4072 Fragments arranged by size: 4072 887 634 419 402 328 266 235 BbeI G_GCGC'C Cuts at: 2974 2974 Size: 7243 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2140 2241 3467 3823 4301 928 Size: 971 241 101 1226 356 478 3870 Fragments arranged by size: 3870 1226 971 478 356 241 101 BbvCI CC'TCA_GC Cuts at: 3739 3739 Size: 7243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3142 3176 5434 5468 1903 Size: 34 1205 34 2258 34 3678 Fragments arranged by size: 3678 2258 1205 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3679 5135 6345 3679 Size: 1456 1210 4577 Fragments arranged by size: 4577 1456 1210 BclI T'GATC_A Cuts at: 1548 3429 3654 1548 Size: 1881 225 5137 Fragments arranged by size: 5137 1881 225 BfrBI ATG'CAT Cuts at: 3008 3008 Size: 7243 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2708 4379 4417 Size: 107 122 71 631 605 1036 1671 38 Cuts at: 4417 5854 136 Size: 1437 1525 Fragments arranged by size: 1671 1525 1437 1036 631 605 122 107 71 38 BglII A'GATC_T Cuts at: 3375 3435 7238 3375 Size: 60 3803 3380 Fragments arranged by size: 3803 3380 60 BlpI GC'TnA_GC Cuts at: 3177 4208 4660 3177 Size: 1031 452 5760 Fragments arranged by size: 5760 1031 452 Bme1580I G_kGCm'C Cuts at: 1790 2278 2432 2442 2912 3492 3506 4278 5304 Size: 488 154 10 470 580 14 772 1026 Cuts at: 5304 6460 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1026 772 580 488 470 154 14 10 BmgBI CAC'GTC Cuts at: 1318 4203 1318 Size: 2885 4358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3776 4974 5932 Size: 416 53 83 399 24 2359 1198 958 Cuts at: 5932 442 Size: 1753 Fragments arranged by size: 2359 1753 1198 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7243 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3397 3436 3397 Size: 39 7204 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2143 2217 2322 2330 2414 2540 2588 Size: 842 62 74 105 8 84 126 48 Cuts at: 2588 2860 3869 5903 1239 Size: 272 1009 2034 2579 Fragments arranged by size: 2579 2034 1009 842 272 126 105 84 74 62 48 8 Bpu10I CC'TnA_GC Cuts at: 3739 3885 3739 Size: 146 7097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5368 6233 6531 5368 Size: 865 298 6080 Fragments arranged by size: 6080 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2177 3242 5906 882 Size: 617 678 1065 2664 2219 Fragments arranged by size: 2664 2219 1065 678 617 BsaAI yAC'GTr Cuts at: 493 1204 5034 493 Size: 711 3830 2702 Fragments arranged by size: 3830 2702 711 BsaBI GATnn'nnATC Cuts at: 3434 7237 3434 Size: 3803 3440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2971 5050 5432 6067 Size: 53 83 186 936 1438 2079 382 635 Cuts at: 6067 275 Size: 1451 Fragments arranged by size: 2079 1451 1438 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5676 6074 6348 6495 6906 5676 Size: 398 274 147 411 6013 Fragments arranged by size: 6013 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4445 4475 1178 Size: 30 165 30 3042 30 3946 Fragments arranged by size: 3946 3042 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2276 2377 3515 3727 4514 1186 Size: 892 198 101 1138 212 787 3915 Fragments arranged by size: 3915 1138 892 787 212 198 101 BseYI C'CCAG_C Cuts at: 2049 2533 2614 2646 3169 3750 6446 6942 2049 Size: 484 81 32 523 581 2696 496 2350 Fragments arranged by size: 2696 2350 581 523 496 484 81 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2717 2843 3039 3167 1163 Size: 573 981 126 196 128 5239 Fragments arranged by size: 5239 981 573 196 128 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4693 5454 6482 1540 Size: 275 186 2692 761 1028 2301 Fragments arranged by size: 2692 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3540 5304 5389 6460 3540 Size: 1764 85 1071 4323 Fragments arranged by size: 4323 1764 1071 85 BsmI GAATG_Cn' Cuts at: 3008 3153 4754 4847 3008 Size: 145 1601 93 5404 Fragments arranged by size: 5404 1601 145 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2278 2432 2442 2912 3178 3492 3506 Size: 399 488 154 10 470 266 314 14 Cuts at: 3506 3540 3908 4143 4278 4562 5304 5389 6460 Size: 34 368 235 135 284 742 85 1071 Cuts at: 6460 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 488 470 399 368 314 284 266 235 154 135 85 34 14 10 BspEI T'CCGG_A Cuts at: 6906 6906 Size: 7243 BspHI T'CATG_A Cuts at: 5132 6862 5132 Size: 1730 5513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2184 3933 4598 844 Size: 1340 1749 665 3489 Fragments arranged by size: 3489 1749 1340 665 BsrBI CCG'CTC Cuts at: 1394 1823 4435 5130 1394 Size: 429 2612 695 3507 Fragments arranged by size: 3507 2612 695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5738 5912 65 Size: 1183 4490 174 1396 Fragments arranged by size: 4490 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2527 2584 3545 4369 5887 6074 1700 Size: 110 717 57 961 824 1518 187 2869 Fragments arranged by size: 2869 1518 961 824 717 187 110 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7243 BssHII G'CGCG_C Cuts at: 1925 2045 1925 Size: 120 7123 BssSI C'ACGA_G Cuts at: 5303 6315 5303 Size: 1012 6231 BstAPI GCAn_nnn'nTGC Cuts at: 794 2530 2803 794 Size: 1736 273 5234 Fragments arranged by size: 5234 1736 273 BstBI TT'CG_AA Cuts at: 2839 6047 2839 Size: 3208 4035 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7243 BstXI CCAn_nnnn'nTGG Cuts at: 4135 4540 4135 Size: 405 6838 BstYI r'GATC_y Cuts at: 1972 2241 2356 3375 3435 4065 4573 4940 5325 Size: 269 115 1019 60 630 508 367 385 Cuts at: 5325 5342 6794 6880 6892 7238 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1019 630 508 385 367 346 269 115 86 60 17 12 Bsu36I CC'TnA_GG Cuts at: 2250 2622 3498 2250 Size: 372 876 5995 Fragments arranged by size: 5995 876 372 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5752 Fragments arranged by size: 5752 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6504 Fragments arranged by size: 6504 683 56 BtsI GCAGTG_nn' Cuts at: 2801 3138 3150 4350 4759 5553 5573 2801 Size: 337 12 1200 409 794 20 4471 Fragments arranged by size: 4471 1200 794 409 337 20 12 ClaI AT'CG_AT Cuts at: 1554 2637 1554 Size: 1083 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3753 3788 3853 3888 529 Size: 35 3189 35 65 35 3884 Fragments arranged by size: 3884 3189 65 35 35 35 DraI TTT'AAA Cuts at: 4547 4547 Size: 7243 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3635 6250 809 Size: 507 2319 2615 1802 Fragments arranged by size: 2615 2319 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 2653 3543 Size: 54 1425 50 275 18 372 451 890 Cuts at: 3543 3954 4530 4690 5579 7208 8 Size: 411 576 160 889 1629 43 Fragments arranged by size: 1629 1425 890 889 576 451 411 372 275 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4690 1537 Size: 275 2878 4090 Fragments arranged by size: 4090 2878 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 2948 3164 5173 1353 Size: 1058 537 216 2009 3423 Fragments arranged by size: 3423 2009 1058 537 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3799 5830 6204 6350 387 Size: 1064 138 2210 2031 374 146 1280 Fragments arranged by size: 2210 2031 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2143 2217 2322 2330 2414 2540 Size: 393 449 62 74 105 8 84 126 Cuts at: 2540 2588 2860 3869 4426 4442 5306 5903 6690 Size: 48 272 1009 557 16 864 597 787 Cuts at: 6690 6958 1239 Size: 268 1524 Fragments arranged by size: 1524 1009 864 787 597 557 449 393 272 268 126 105 84 74 62 48 16 8 EcoO109I rG'GnC_Cy Cuts at: 2085 2275 2314 2618 2626 2890 2908 2950 3022 Size: 190 39 304 8 264 18 42 72 Cuts at: 3022 3206 3502 3503 3637 2085 Size: 184 296 1 134 5691 Fragments arranged by size: 5691 304 296 264 190 184 134 72 42 39 18 8 1 EcoRI G'AATT_C Cuts at: 2454 4552 4930 2454 Size: 2098 378 4767 Fragments arranged by size: 4767 2098 378 EcoRV GAT'ATC Cuts at: 2965 2965 Size: 7243 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4507 4507 Size: 7243 FspI TGC'GCA Cuts at: 3575 5749 3575 Size: 2174 5069 HaeII r_GCGC'y Cuts at: 1678 2848 2974 6390 1678 Size: 1170 126 3416 2531 Fragments arranged by size: 3416 2531 1170 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2233 2265 2774 2806 3241 Size: 32 135 32 661 32 509 32 435 Cuts at: 3241 3273 4089 4121 5884 5916 5958 5990 1373 Size: 32 816 32 1763 32 42 32 2626 Fragments arranged by size: 2626 1763 816 661 509 435 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4587 4833 669 Size: 988 281 2649 246 3079 Fragments arranged by size: 3079 2649 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7243 HpaI GTT'AAC Cuts at: 4833 4833 Size: 7243 KasI G'GCGC_C Cuts at: 2970 2970 Size: 7243 KpnI G_GTAC'C Cuts at: 3552 4568 3552 Size: 1016 6227 MfeI C'AATT_G Cuts at: 4842 4842 Size: 7243 MluI A'CGCG_T Cuts at: 7119 7119 Size: 7243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3102 3756 6357 6541 1305 Size: 1797 654 2601 184 2007 Fragments arranged by size: 2601 2007 1797 654 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2204 4532 10 Size: 54 1425 343 372 2328 2721 Fragments arranged by size: 2721 2328 1425 372 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2672 3143 3794 3992 4028 4133 Size: 648 1326 180 471 651 198 36 105 Cuts at: 4133 4269 4538 5201 5560 5719 518 Size: 136 269 663 359 159 2042 Fragments arranged by size: 2042 1326 663 651 648 471 359 269 198 180 159 136 105 36 MspA1I CmG'CkG Cuts at: 1606 2561 4087 4212 4414 4659 5336 6484 6729 Size: 955 1526 125 202 245 677 1148 245 Cuts at: 6729 1606 Size: 2120 Fragments arranged by size: 2120 1526 1148 955 677 245 245 202 125 NaeI GCC'GGC Cuts at: 4371 4371 Size: 7243 NarI GG'CG_CC Cuts at: 2971 2971 Size: 7243 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5752 Fragments arranged by size: 5752 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7243 NgoMIV G'CCGG_C Cuts at: 4369 4369 Size: 7243 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7243 NotI GC'GGCC_GC Cuts at: 4690 4690 Size: 7243 NsiI A_TGCA'T Cuts at: 3010 3010 Size: 7243 NspI r_CATG'y Cuts at: 1132 1229 4309 4601 6146 1132 Size: 97 3080 292 1545 2229 Fragments arranged by size: 3080 2229 1545 292 97 PasI CC'CwG_GG Cuts at: 1348 2317 4049 1348 Size: 969 1732 4542 Fragments arranged by size: 4542 1732 969 PciI A'CATG_T Cuts at: 6142 6142 Size: 7243 PflMI CCAn_nnn'nTGG Cuts at: 1108 3025 3766 1108 Size: 1917 741 4585 Fragments arranged by size: 4585 1917 741 PfoI T'CCnGG_A Cuts at: 2517 2751 3441 3846 4068 2517 Size: 234 690 405 222 5692 Fragments arranged by size: 5692 690 405 234 222 PmeI GTTT'AAAC Cuts at: 4547 4547 Size: 7243 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2672 2704 5312 5344 6825 6857 2672 Size: 32 2608 32 1481 32 3058 Fragments arranged by size: 3058 2608 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2618 3022 3206 3637 2618 Size: 404 184 431 6224 Fragments arranged by size: 6224 431 404 184 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7243 PsiI TTA'TAA Cuts at: 3468 4813 3468 Size: 1345 5898 PspOMI G'GGCC_C Cuts at: 2274 2908 3502 2274 Size: 634 594 6015 Fragments arranged by size: 6015 634 594 PstI C_TGCA'G Cuts at: 830 2338 2553 2762 2928 3145 4060 4595 830 Size: 1508 215 209 166 217 915 535 3478 Fragments arranged by size: 3478 1508 915 535 217 215 209 166 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7243 SalI G'TCGA_C Cuts at: 1936 4585 1936 Size: 2649 4594 SanDI GG'GwC_CC Cuts at: 3637 3637 Size: 7243 SapI GCTCTTCn'nnn_ Cuts at: 2411 2948 2411 Size: 537 6706 SbfI CC_TGCA'GG Cuts at: 4595 4595 Size: 7243 ScaI AGT'ACT Cuts at: 5491 7203 5491 Size: 1712 5531 SfcI C'TryA_G Cuts at: 826 1045 2334 2549 2758 2924 3141 3455 4056 Size: 219 1289 215 209 166 217 314 601 Cuts at: 4056 4591 5726 6407 6598 826 Size: 535 1135 681 191 1471 Fragments arranged by size: 1471 1289 1135 681 601 535 314 219 217 215 209 191 166 SfiI GGCCn_nnn'nGGCC Cuts at: 4379 4379 Size: 7243 SfoI GGC'GCC Cuts at: 2972 2972 Size: 7243 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7243 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7243 SmaI CCC'GGG Cuts at: 1872 3746 4570 1872 Size: 1874 824 4545 Fragments arranged by size: 4545 1874 824 SmlI C'TyrA_G Cuts at: 820 1017 1943 5347 6248 6510 820 Size: 197 926 3404 901 262 1553 Fragments arranged by size: 3404 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5034 493 Size: 4541 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7243 SphI G_CATG'C Cuts at: 1132 4309 4601 1132 Size: 3177 292 3774 Fragments arranged by size: 3774 3177 292 SspI AAT'ATT Cuts at: 5 52 5167 5 Size: 47 5115 2081 Fragments arranged by size: 5115 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7243 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 4482 4682 513 Size: 552 939 150 2328 200 3074 Fragments arranged by size: 3074 2328 939 552 200 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2041 2271 2660 3465 3710 3777 3935 Size: 1255 461 230 389 805 245 67 158 Cuts at: 3935 4022 4270 4281 4342 5270 5287 5440 5625 Size: 87 248 11 61 928 17 153 185 Cuts at: 5625 6070 325 Size: 445 1498 Fragments arranged by size: 1498 1255 928 805 461 445 389 248 245 230 185 158 153 87 67 61 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3264 4168 5489 Size: 274 80 33 51 1443 1287 904 1321 Cuts at: 5489 7201 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1321 1287 904 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4445 4477 7203 7235 4445 Size: 32 2726 32 4453 Fragments arranged by size: 4453 2726 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7243 XbaI T'CTAG_A Cuts at: 3259 4579 3259 Size: 1320 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3655 3655 Size: 7243 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7243 XmaI C'CCGG_G Cuts at: 1870 3744 4568 1870 Size: 1874 824 4545 Fragments arranged by size: 4545 1874 824 XmnI GAAnn'nnTTC Cuts at: 2466 5372 2466 Size: 2906 4337 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5051 276 Size: 53 83 186 936 3517 2468 Fragments arranged by size: 3517 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SexAI SrfI SwaI