(Linear) (Six-base) MAPSORT of: KIEE0697.seq Check: 804 from: 1 to: 2538 KIEE0697.orf With 182 enzymes: SgfI * February 18, 2010 15:07 .. Acc65I G'GTAC_C Cuts at: 0 1543 2538 Size: 1543 995 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2421 2437 2538 Size: 2421 16 101 Fragments arranged by size: 2421 101 16 AflIII A'CryG_T Cuts at: 0 1472 2538 Size: 1472 1066 AhdI GACnn_n'nnGTC Cuts at: 0 1250 2098 2538 Size: 1250 848 440 Fragments arranged by size: 1250 848 440 AleI CACnn'nnGTG Cuts at: 0 487 1138 1987 2538 Size: 487 651 849 551 Fragments arranged by size: 849 651 551 487 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 778 2538 Size: 778 1760 AlwNI CAG_nnn'CTG Cuts at: 0 21 366 798 1020 1179 2050 2460 2538 Size: 21 345 432 222 159 871 410 78 Fragments arranged by size: 871 432 410 345 222 159 78 21 ApaI G_GGCC'C Cuts at: 0 273 907 1501 2538 Size: 273 634 594 1037 Fragments arranged by size: 1037 634 594 273 ApoI r'AATT_y Cuts at: 0 449 2538 Size: 449 2089 AvaI C'yCGr_G Cuts at: 0 1739 2538 Size: 1739 799 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2239 2272 2538 Size: 2239 33 266 Fragments arranged by size: 2239 266 33 BamHI G'GATC_C Cuts at: 0 351 2538 Size: 351 2187 BanI G'GyrC_C Cuts at: 0 582 810 965 1482 1543 1704 2538 Size: 582 228 155 517 61 161 834 Fragments arranged by size: 834 582 517 228 161 155 61 BanII G_rGCy'C Cuts at: 0 273 907 1173 1501 1903 2138 2538 Size: 273 634 266 328 402 235 400 Fragments arranged by size: 634 402 400 328 273 266 235 BbeI G_GCGC'C Cuts at: 0 969 2538 Size: 969 1569 BbsI GAAGACnn'nnnn_ Cuts at: 0 135 236 1462 1818 2296 2538 Size: 135 101 1226 356 478 242 Fragments arranged by size: 1226 478 356 242 135 101 BbvCI CC'TCA_GC Cuts at: 0 1734 2538 Size: 1734 804 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1137 1171 2538 Size: 1137 34 1367 Fragments arranged by size: 1367 1137 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1674 2538 Size: 1674 864 BclI T'GATC_A Cuts at: 0 1424 1649 2538 Size: 1424 225 889 Fragments arranged by size: 1424 889 225 BfrBI ATG'CAT Cuts at: 0 1003 2538 Size: 1003 1535 BglI GCCn_nnn'nGGC Cuts at: 0 703 2374 2412 2538 Size: 703 1671 38 126 Fragments arranged by size: 1671 703 126 38 BglII A'GATC_T Cuts at: 0 1370 1430 2538 Size: 1370 60 1108 Fragments arranged by size: 1370 1108 60 BlpI GC'TnA_GC Cuts at: 0 1172 2203 2538 Size: 1172 1031 335 Fragments arranged by size: 1172 1031 335 Bme1580I G_kGCm'C Cuts at: 0 273 427 437 907 1487 1501 2273 2538 Size: 273 154 10 470 580 14 772 265 Fragments arranged by size: 772 580 470 273 265 154 14 10 BmgBI CAC'GTC Cuts at: 0 2198 2538 Size: 2198 340 BmrI ACTGGGnnnn_n' Cuts at: 0 1771 2538 Size: 1771 767 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1392 1431 2538 Size: 1392 39 1107 Fragments arranged by size: 1392 1107 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 138 212 317 325 409 535 583 Size: 76 62 74 105 8 84 126 48 Cuts at: 583 855 1864 2538 Size: 272 1009 674 Fragments arranged by size: 1009 674 272 126 105 84 76 74 62 48 8 Bpu10I CC'TnA_GC Cuts at: 0 1734 1880 2538 Size: 1734 146 658 Fragments arranged by size: 1734 658 146 BsaI GGTCTCn'nnnn_ Cuts at: 0 172 1237 2538 Size: 172 1065 1301 Fragments arranged by size: 1301 1065 172 BsaBI GATnn'nnATC Cuts at: 0 1429 2538 Size: 1429 1109 BsaHI Gr'CG_yC Cuts at: 0 966 2538 Size: 966 1572 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2440 2470 2538 Size: 2440 30 68 Fragments arranged by size: 2440 68 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 73 271 372 1510 1722 2509 2538 Size: 73 198 101 1138 212 787 29 Fragments arranged by size: 1138 787 212 198 101 73 29 BseYI C'CCAG_C Cuts at: 0 44 528 609 641 1164 1745 2538 Size: 44 484 81 32 523 581 793 Fragments arranged by size: 793 581 523 484 81 44 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 838 1034 1162 2538 Size: 712 126 196 128 1376 Fragments arranged by size: 1376 712 196 128 126 BsiHKAI G_wGCw'C Cuts at: 0 1535 2538 Size: 1535 1003 BsmI GAATG_Cn' Cuts at: 0 1003 1148 2538 Size: 1003 145 1390 Fragments arranged by size: 1390 1003 145 Bsp1286I G_dGCh'C Cuts at: 0 273 427 437 907 1173 1487 1501 1535 Size: 273 154 10 470 266 314 14 34 Cuts at: 1535 1903 2138 2273 2538 Size: 368 235 135 265 Fragments arranged by size: 470 368 314 273 266 265 235 154 135 34 14 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 179 1928 2538 Size: 179 1749 610 Fragments arranged by size: 1749 610 179 BsrBI CCG'CTC Cuts at: 0 2430 2538 Size: 2430 108 BsrFI r'CCGG_y Cuts at: 0 522 579 1540 2364 2538 Size: 522 57 961 824 174 Fragments arranged by size: 961 824 522 174 57 BssHII G'CGCG_C Cuts at: 0 40 2538 Size: 40 2498 BstAPI GCAn_nnn'nTGC Cuts at: 0 525 798 2538 Size: 525 273 1740 Fragments arranged by size: 1740 525 273 BstBI TT'CG_AA Cuts at: 0 834 2538 Size: 834 1704 BstXI CCAn_nnnn'nTGG Cuts at: 0 2130 2538 Size: 2130 408 BstYI r'GATC_y Cuts at: 0 236 351 1370 1430 2060 2538 Size: 236 115 1019 60 630 478 Fragments arranged by size: 1019 630 478 236 115 60 Bsu36I CC'TnA_GG Cuts at: 0 245 617 1493 2538 Size: 245 372 876 1045 Fragments arranged by size: 1045 876 372 245 BtsI GCAGTG_nn' Cuts at: 0 796 1133 1145 2345 2538 Size: 796 337 12 1200 193 Fragments arranged by size: 1200 796 337 193 12 ClaI AT'CG_AT Cuts at: 0 632 2538 Size: 632 1906 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1748 1783 1848 1883 2538 Size: 1748 35 65 35 655 Fragments arranged by size: 1748 655 65 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1630 2538 Size: 1630 908 EaeI y'GGCC_r Cuts at: 0 197 648 1538 1949 2525 2538 Size: 197 451 890 411 576 13 Fragments arranged by size: 890 576 451 411 197 13 EarI CTCTTCn'nnn_ Cuts at: 0 406 943 1159 2538 Size: 406 537 216 1379 Fragments arranged by size: 1379 537 406 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1794 2538 Size: 1794 744 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 138 212 317 325 409 535 583 Size: 76 62 74 105 8 84 126 48 Cuts at: 583 855 1864 2421 2437 2538 Size: 272 1009 557 16 101 Fragments arranged by size: 1009 557 272 126 105 101 84 76 74 62 48 16 8 EcoO109I rG'GnC_Cy Cuts at: 0 80 270 309 613 621 885 903 945 Size: 80 190 39 304 8 264 18 42 Cuts at: 945 1017 1201 1497 1498 1632 2538 Size: 72 184 296 1 134 906 Fragments arranged by size: 906 304 296 264 190 184 134 80 72 42 39 18 8 1 EcoRI G'AATT_C Cuts at: 0 449 2538 Size: 449 2089 EcoRV GAT'ATC Cuts at: 0 960 2538 Size: 960 1578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2502 2538 Size: 2502 36 FspI TGC'GCA Cuts at: 0 1570 2538 Size: 1570 968 HaeII r_GCGC'y Cuts at: 0 843 969 2538 Size: 843 126 1569 Fragments arranged by size: 1569 843 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 228 260 769 801 1236 1268 2084 2116 Size: 228 32 509 32 435 32 816 32 Cuts at: 2116 2538 Size: 422 Fragments arranged by size: 816 509 435 422 228 32 32 32 32 KasI G'GCGC_C Cuts at: 0 965 2538 Size: 965 1573 KpnI G_GTAC'C Cuts at: 0 1547 2538 Size: 1547 991 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1097 1751 2538 Size: 1097 654 787 Fragments arranged by size: 1097 787 654 MscI TGG'CCA Cuts at: 0 199 2527 2538 Size: 199 2328 11 Fragments arranged by size: 2328 199 11 MslI CAynn'nnrTG Cuts at: 0 487 667 1138 1789 1987 2023 2128 2264 Size: 487 180 471 651 198 36 105 136 Cuts at: 2264 2533 2538 Size: 269 5 Fragments arranged by size: 651 487 471 269 198 180 136 105 36 5 MspA1I CmG'CkG Cuts at: 0 556 2082 2207 2409 2538 Size: 556 1526 125 202 129 Fragments arranged by size: 1526 556 202 129 125 NaeI GCC'GGC Cuts at: 0 2366 2538 Size: 2366 172 NarI GG'CG_CC Cuts at: 0 966 2538 Size: 966 1572 NgoMIV G'CCGG_C Cuts at: 0 2364 2538 Size: 2364 174 NsiI A_TGCA'T Cuts at: 0 1005 2538 Size: 1005 1533 NspI r_CATG'y Cuts at: 0 2304 2538 Size: 2304 234 PasI CC'CwG_GG Cuts at: 0 312 2044 2538 Size: 312 1732 494 Fragments arranged by size: 1732 494 312 PflMI CCAn_nnn'nTGG Cuts at: 0 1020 1761 2538 Size: 1020 741 777 Fragments arranged by size: 1020 777 741 PfoI T'CCnGG_A Cuts at: 0 512 746 1436 1841 2063 2538 Size: 512 234 690 405 222 475 Fragments arranged by size: 690 512 475 405 234 222 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 667 699 2538 Size: 667 32 1839 Fragments arranged by size: 1839 667 32 PpuMI rG'GwC_Cy Cuts at: 0 613 1017 1201 1632 2538 Size: 613 404 184 431 906 Fragments arranged by size: 906 613 431 404 184 PsiI TTA'TAA Cuts at: 0 1463 2538 Size: 1463 1075 PspOMI G'GGCC_C Cuts at: 0 269 903 1497 2538 Size: 269 634 594 1041 Fragments arranged by size: 1041 634 594 269 PstI C_TGCA'G Cuts at: 0 333 548 757 923 1140 2055 2538 Size: 333 215 209 166 217 915 483 Fragments arranged by size: 915 483 333 217 215 209 166 SanDI GG'GwC_CC Cuts at: 0 1632 2538 Size: 1632 906 SapI GCTCTTCn'nnn_ Cuts at: 0 406 943 2538 Size: 406 537 1595 Fragments arranged by size: 1595 537 406 SfcI C'TryA_G Cuts at: 0 329 544 753 919 1136 1450 2051 2538 Size: 329 215 209 166 217 314 601 487 Fragments arranged by size: 601 487 329 314 217 215 209 166 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2374 2538 Size: 2374 164 SfoI GGC'GCC Cuts at: 0 967 2538 Size: 967 1571 SmaI CCC'GGG Cuts at: 0 1741 2538 Size: 1741 797 SphI G_CATG'C Cuts at: 0 2304 2538 Size: 2304 234 StyI C'CwwG_G Cuts at: 0 149 2477 2538 Size: 149 2328 61 Fragments arranged by size: 2328 149 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 36 266 655 1460 1705 1772 1930 2017 Size: 36 230 389 805 245 67 158 87 Cuts at: 2017 2265 2276 2337 2538 Size: 248 11 61 201 Fragments arranged by size: 805 389 248 245 230 201 158 87 67 61 36 11 TatI w'GTAC_w Cuts at: 0 1259 2163 2538 Size: 1259 904 375 Fragments arranged by size: 1259 904 375 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2440 2472 2538 Size: 2440 32 66 Fragments arranged by size: 2440 66 32 XbaI T'CTAG_A Cuts at: 0 1254 2538 Size: 1254 1284 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1650 2538 Size: 1650 888 XmaI C'CCGG_G Cuts at: 0 1739 2538 Size: 1739 799 XmnI GAAnn'nnTTC Cuts at: 0 461 2538 Size: 461 2077 Enzymes that do cut: Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstYI Bsu36I BtsI ClaI CspCI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI PsiI PspOMI PstI SanDI SapI SfcI SfiI SfoI SmaI SphI StyI TaqII TatI TstI XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AloI ApaLI AscI AseI AsiSI AvrII BmtI BpuEI BsaAI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstEII BstZ17I BtgI BtgZI DraI DraIII EagI EcoICRI EcoNI FseI FspAI HincII HindIII HpaI MfeI MluI NcoI NdeI NheI NotI NruI PacI PciI PmeI PmlI PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XhoI ZraI