(Circular) (Six-base) MAPSORT of: pFN21AB9297.seq Check: 9516 from: 1 to: 5647 pFN21AB9297 5647 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2530 2530 Size: 5647 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2961 3710 5094 5362 1632 Size: 422 907 749 1384 268 1917 Fragments arranged by size: 1917 1384 907 749 422 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 2625 4546 5523 1203 Size: 1422 1921 977 1327 Fragments arranged by size: 1921 1422 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 2314 3390 4962 2314 Size: 1076 1572 2999 Fragments arranged by size: 2999 1572 1076 ApaI G_GGCC'C Cuts at: 2330 2330 Size: 5647 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2803 2912 2956 3182 3334 5443 1493 Size: 1310 109 44 226 152 2109 1697 Fragments arranged by size: 2109 1697 1310 226 152 109 44 AseI AT'TA_AT Cuts at: 160 1032 2798 4201 160 Size: 872 1766 1403 1606 Fragments arranged by size: 1766 1606 1403 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2286 2323 2921 2972 1870 Size: 73 343 37 598 51 4545 Fragments arranged by size: 4545 598 343 73 51 37 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2968 4423 618 Size: 325 217 627 1181 1455 1842 Fragments arranged by size: 1842 1455 1181 627 325 217 BanII G_rGCy'C Cuts at: 1391 2330 2507 2966 1391 Size: 939 177 459 4072 Fragments arranged by size: 4072 939 459 177 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2062 928 Size: 971 163 4513 Fragments arranged by size: 4513 971 163 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 2076 1548 Size: 528 5119 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4258 136 Size: 107 122 71 631 605 2586 1525 Fragments arranged by size: 2586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2655 5642 2655 Size: 2987 2660 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2330 3708 4864 1790 Size: 540 1378 1156 2573 Fragments arranged by size: 2573 1378 1156 540 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2293 3378 4336 Size: 416 53 83 399 24 876 1085 958 Cuts at: 4336 442 Size: 1753 Fragments arranged by size: 1753 1085 958 876 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2336 4307 1239 Size: 806 291 1971 2579 Fragments arranged by size: 2579 1971 806 291 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 3772 4637 4935 2213 Size: 1559 865 298 2925 Fragments arranged by size: 2925 1559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2628 3438 493 Size: 711 1424 810 2702 Fragments arranged by size: 2702 1424 810 711 BsaBI GATnn'nnATC Cuts at: 2474 2703 5641 2474 Size: 229 2938 2480 Fragments arranged by size: 2938 2480 229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3454 3836 4471 275 Size: 53 83 186 936 1921 382 635 1451 Fragments arranged by size: 1921 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2257 4080 4478 4752 4899 5310 2257 Size: 1823 398 274 147 411 2594 Fragments arranged by size: 2594 1823 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2239 2269 1178 Size: 30 165 30 836 30 4556 Fragments arranged by size: 4556 836 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 1186 Size: 1294 4353 BseYI C'CCAG_C Cuts at: 4850 5346 4850 Size: 496 5151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5074 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2507 3708 3793 4864 2507 Size: 1201 85 1071 3290 Fragments arranged by size: 3290 1201 1071 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2507 2966 3708 3793 4864 1391 Size: 399 540 177 459 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 459 399 177 85 BspEI T'CCGG_A Cuts at: 2257 5310 2257 Size: 3053 2594 BspHI T'CATG_A Cuts at: 2073 3536 5266 2073 Size: 1463 1730 2454 Fragments arranged by size: 2454 1730 1463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2530 3002 844 Size: 1686 472 3489 Fragments arranged by size: 3489 1686 472 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 2040 2599 96 Size: 1944 559 3144 Fragments arranged by size: 3144 1944 559 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 2462 3707 4719 2462 Size: 1245 1012 3390 Fragments arranged by size: 3390 1245 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2815 794 Size: 2021 3626 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2655 2977 3344 3729 3746 5198 5284 5296 Size: 683 322 367 385 17 1452 86 12 Cuts at: 5296 5642 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 683 385 367 346 322 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2091 2524 513 Size: 552 1026 433 3636 Fragments arranged by size: 3636 1026 552 433 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 3163 3957 3977 3163 Size: 794 20 4833 Fragments arranged by size: 4833 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2754 2951 2754 Size: 197 5450 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2571 3094 Size: 54 1425 50 275 18 264 477 523 Cuts at: 3094 3983 5612 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 523 477 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3577 1353 Size: 2224 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2505 2505 Size: 5647 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2054 2336 2961 3710 4307 5094 Size: 393 413 9 282 625 749 597 787 Cuts at: 5094 5362 1239 Size: 268 1524 Fragments arranged by size: 1524 787 749 625 597 413 393 282 268 9 EcoO109I rG'GnC_Cy Cuts at: 2311 2311 Size: 5647 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2091 4794 1678 Size: 413 2703 2531 Fragments arranged by size: 2703 2531 413 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 4288 4320 4362 Size: 32 135 32 557 32 2127 32 42 Cuts at: 4362 4394 1373 Size: 32 2626 Fragments arranged by size: 2626 2127 557 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 2762 748 Size: 2014 3633 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 10 Size: 54 1425 343 264 3561 Fragments arranged by size: 3561 1425 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 3605 3964 4123 518 Size: 648 2439 359 159 2042 Fragments arranged by size: 2439 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2277 3063 3740 4888 5133 1606 Size: 671 786 677 1148 245 2120 Fragments arranged by size: 2120 1148 786 677 671 245 NcoI C'CATG_G Cuts at: 513 1065 2091 513 Size: 552 1026 4069 Fragments arranged by size: 4069 1026 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 3005 4550 1132 Size: 97 1776 1545 2229 Fragments arranged by size: 2229 1776 1545 97 PasI CC'CwG_GG Cuts at: 1348 2113 2579 1348 Size: 765 466 4416 Fragments arranged by size: 4416 765 466 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 2314 2646 1108 Size: 1206 332 4109 Fragments arranged by size: 4109 1206 332 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 2721 3217 2721 Size: 496 5151 PspOMI G'GGCC_C Cuts at: 2326 2326 Size: 5647 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 SacI G_AGCT'C Cuts at: 2507 2507 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2493 2995 4130 4811 5002 826 Size: 219 1448 502 1135 681 191 1471 Fragments arranged by size: 1471 1448 1135 681 502 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 3751 4652 4914 820 Size: 197 926 285 1523 901 262 1553 Fragments arranged by size: 1553 1523 926 901 285 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5647 StyI C'CwwG_G Cuts at: 513 1065 2091 3086 513 Size: 552 1026 995 3074 Fragments arranged by size: 3074 1026 995 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2312 2420 3674 3691 3844 4029 4474 Size: 1255 732 108 1254 17 153 185 445 Cuts at: 4474 325 Size: 1498 Fragments arranged by size: 1498 1255 1254 732 445 185 153 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 2264 2412 Size: 274 80 33 51 1443 63 224 148 Cuts at: 2412 2599 3893 5605 96 Size: 187 1294 1712 138 Fragments arranged by size: 1712 1443 1294 274 224 187 148 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2239 2271 5607 5639 2239 Size: 32 3336 32 2247 Fragments arranged by size: 3336 2247 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 2378 3776 2378 Size: 1398 4249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI