Restriction map for
pFN21AB9189
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HpaII KasI
KpnI KspI MaeI MfeI MluI
MroI MspI MunI MwoI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 102 GTCTAC GT^[AC][GT]AC
AccII 835 CGCG CG^CG
AclI 407 AACGTT AA^CGTT
AflII 1066 CTTAAG C^TTAAG
ApaI 1238 GGGCCC GGGCC^C
AvaII 624 GGTCC G^G[AT]CC
BalI 57 TGGCCA TGG^CCA
BamHI 437 GGATCC G^GATCC
BanII 1238 GGGCCC G[GA]GC[CT]^C
BfrI 1066 CTTAAG C^TTAAG
BmyI 1238 GGGCCC G[AGT]GC[ACT]^C
BsaBI 825 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1286I 1238 GGGCCC G[AGT]GC[ACT]^C
BspHI 599 TCATGA T^CATGA
BstBI 38 TTCGAA TT^CGAA
BstEII 498 GGTCACC G^GT[ACGT]ACC
BstF5I 129 CATCC GGATG(2/0)
BstPI 498 GGTCACC G^GT[ACGT]ACC
BstUI 835 CGCG CG^CG
DdeI 1205 CTCAG C^T[ACGT]AG
DraII 1237 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 85 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 384 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1237 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 498 GGTCACC G^GT[ACGT]ACC
EcoRI 985 GAATTC G^AATTC
FauI 872 CCCGC CCCGC(4/6)
FokI 129 CATCC GGATG(9/13)
HgaI 893 GACGC GACGC(5/10)
HpaI 285 GTTAAC GTT^AAC
MamI 825 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 57 TGGCCA TGG^CCA
MscI 57 TGGCCA TGG^CCA
MslI 165 CACGTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 835 CGCG CG^CG
NdeI 965 CATATG CA^TATG
NspV 38 TTCGAA TT^CGAA
PleI 864 GACTC GAGTC(4/5)
Psp1406I 407 AACGTT AA^CGTT
PstI 1000 CTGCAG CTGCA^G
PvuII 88 CAGCTG CAG^CTG
RcaI 599 TCATGA T^CATGA
SapI 31 GAAGAGC GCTCTTC(1/4)
SexAI 225 ACCAGGT A^CC[AT]GGT
SfuI 38 TTCGAA TT^CGAA
StuI 377 AGGCCT AGG^CCT
TfiI 116 GATTC G^A[AT]TC
XcmI 503 CCATGAAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbsI 1144 GAAGAC GAAGAC(2/6)
953 GTCTTC
BbvI 713 GCAGC GCAGC(8/12)
1198 GCAGC
BpuAI 1144 GAAGAC GAAGAC(2/6)
953 GTCTTC
BsaI 18 GAGACC GGTCTC(1/5)
314 GAGACC
BseRI 1165 GAGGAG GAGGAG(10/8)
1168 GAGGAG
BsgI 577 CTGCAC GTGCAG(16/14)
778 GTGCAG
BsmFI 486 GTCCC GGGAC(10/14)
1218 GTCCC
BspMI 383 ACCTGC ACCTGC(4/8)
780 GCAGGT
BssSI 432 CACGAG CACGAG(-5/-1)
1090 CACGAG
BstXI 164 CCACGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1227 CCAGCAATCTGG
BstYI 437 GGATCC [GA]^GATC[CT]
492 GGATCT
Cac8I 445 GCCTGC GC[ACGT]^[ACGT]GC
1276 GCAAGC
HindIII 901 AAGCTT A^AGCTT
1278 AAGCTT
HinfI 116 GATTC G^A[ACGT]TC
864 GACTC
MaeII 166 ACGT A^CGT
408 ACGT
MflI 437 GGATCC [GA]^GATC[CT]
492 GGATCT
MspA1I 88 CAGCTG C[AC]G^C[GT]G
1199 CAGCGG
SfaNI 364 GATGC GCATC(5/9)
825 GATGC
SfcI 1000 CTGCAG C^T[GA][CT]AG
1213 CTACAG
TseI 713 GCAGC G^C[AT]GC
1198 GCAGC
Tsp45I 203 GTGAC ^GT[GC]AC
499 GTCAC
XhoII 437 GGATCC [GA]^GATC[CT]
492 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BsaJI BsiYI BslI
BsmAI BsrDI BsrI BssKI BstNI
Cfr13I DpnII DsaI EaeI EarI
EcoRII Fnu4HI HaeIII HincII HindII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SmlI TaqI Tru9I Tsp509I
TspRI TthHB8I XmnI