(Circular) (Six-base) MAPSORT of: pFN21AB9189.seq Check: 6634 from: 1 to: 5992 pFN21AB9189 5992 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2108 3335 1937 Size: 171 1227 4594 Fragments arranged by size: 4594 1227 171 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 2413 4119 4492 1563 Size: 850 1706 373 3063 Fragments arranged by size: 3063 1706 850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 2306 2938 2945 4055 5439 5707 1632 Size: 617 57 632 7 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1110 632 617 268 57 7 AflII C'TTAA_G Cuts at: 820 1017 3071 820 Size: 197 2054 3741 Fragments arranged by size: 3741 2054 197 AflIII A'CryG_T Cuts at: 1203 4891 5868 1203 Size: 3688 977 1327 Fragments arranged by size: 3688 1327 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2171 2203 2171 Size: 32 5960 AlwNI CAG_nnn'CTG Cuts at: 3735 5307 3735 Size: 1572 4420 ApaI G_GGCC'C Cuts at: 3247 3247 Size: 5992 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 2198 2588 2990 3301 3527 3679 5788 1493 Size: 705 390 402 311 226 152 2109 1697 Fragments arranged by size: 2109 1697 705 402 390 311 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 3317 1870 Size: 73 1374 4545 Fragments arranged by size: 4545 1374 73 BamHI G'GATC_C Cuts at: 2442 3322 3689 2442 Size: 880 367 4745 Fragments arranged by size: 4745 880 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3313 4768 618 Size: 325 217 627 1526 1455 1842 Fragments arranged by size: 1842 1526 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3247 3311 1391 Size: 1856 64 4072 Fragments arranged by size: 4072 1856 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2951 3156 928 Size: 971 1052 205 3764 Fragments arranged by size: 3764 1052 971 205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4183 4217 1903 Size: 34 2246 34 3678 Fragments arranged by size: 3678 2246 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4603 136 Size: 107 122 71 631 605 2931 1525 Fragments arranged by size: 2931 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 3247 4053 5209 1790 Size: 1457 806 1156 2573 Fragments arranged by size: 2573 1457 1156 806 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2368 3723 4681 Size: 416 53 83 399 24 951 1355 958 Cuts at: 4681 442 Size: 1753 Fragments arranged by size: 1753 1355 958 951 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4652 1239 Size: 3413 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2100 3188 4117 4982 5280 2100 Size: 1088 929 865 298 2812 Fragments arranged by size: 2812 1088 929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2017 2313 4655 882 Size: 617 518 296 2342 2219 Fragments arranged by size: 2342 2219 617 518 296 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 2834 5986 2834 Size: 3152 2840 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3799 4181 4816 275 Size: 53 83 186 936 2266 382 635 1451 Fragments arranged by size: 2266 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5767 Fragments arranged by size: 5767 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3185 3188 1186 Size: 1999 3 3990 Fragments arranged by size: 3990 1999 3 BseYI C'CCAG_C Cuts at: 2212 3231 3246 5195 5691 2212 Size: 1019 15 1949 496 2513 Fragments arranged by size: 2513 1949 1019 496 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2567 2804 1163 Size: 573 831 237 4351 Fragments arranged by size: 4351 831 573 237 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 4203 5231 1540 Size: 275 186 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4053 4138 5209 4053 Size: 85 1071 4836 Fragments arranged by size: 4836 1071 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3247 3311 4053 4138 5209 1391 Size: 399 1457 64 742 85 1071 2174 Fragments arranged by size: 2174 1457 1071 742 399 85 64 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 2604 3881 5611 2604 Size: 1277 1730 2985 Fragments arranged by size: 2985 1730 1277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2397 2776 3347 844 Size: 1553 379 571 3489 Fragments arranged by size: 3489 1553 571 379 BsrBI CCG'CTC Cuts at: 1394 1823 3879 1394 Size: 429 2056 3507 Fragments arranged by size: 3507 2056 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2281 2565 2629 2803 4487 4661 65 Size: 1183 1033 284 64 174 1684 174 1396 Fragments arranged by size: 1684 1396 1183 1033 284 174 174 64 BsrFI r'CCGG_y Cuts at: 1700 1810 4636 4823 1700 Size: 110 2826 187 2869 Fragments arranged by size: 2869 2826 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 2437 3095 4052 5064 2437 Size: 658 957 1012 3365 Fragments arranged by size: 3365 1012 957 658 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5992 BstBI TT'CG_AA Cuts at: 2044 4796 2044 Size: 2752 3240 BstEII G'GTnAC_C Cuts at: 1184 2503 1184 Size: 1319 4673 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2176 3239 2011 Size: 165 1063 4764 Fragments arranged by size: 4764 1063 165 BstYI r'GATC_y Cuts at: 1972 2442 2497 3322 3689 4074 4091 5543 5629 Size: 470 55 825 367 385 17 1452 86 Cuts at: 5629 5641 5987 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 825 470 385 367 346 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2016 2307 513 Size: 552 939 6 6 291 4198 Fragments arranged by size: 4198 939 552 291 6 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5253 Fragments arranged by size: 5253 683 56 BtsI GCAGTG_nn' Cuts at: 2715 3508 4302 4322 2715 Size: 793 794 20 4385 Fragments arranged by size: 4385 794 793 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2096 4999 809 Size: 507 780 2903 1802 Fragments arranged by size: 2903 1802 780 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2013 2062 Size: 54 1425 50 275 18 177 6 49 Cuts at: 2062 3439 4328 5957 8 Size: 1377 889 1629 43 Fragments arranged by size: 1629 1425 1377 889 275 177 54 50 49 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2426 2721 2907 3922 1353 Size: 678 395 295 186 1015 3423 Fragments arranged by size: 3423 1015 678 395 295 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4579 4953 5099 387 Size: 1064 138 2990 374 146 1280 Fragments arranged by size: 2990 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2306 2938 2945 4055 4652 5439 Size: 393 617 57 632 7 1110 597 787 Cuts at: 5439 5707 1239 Size: 268 1524 Fragments arranged by size: 1524 1110 787 632 617 597 393 268 57 7 EcoNI CCTnn'n_nnAGG Cuts at: 2393 2393 Size: 5992 EcoO109I rG'GnC_Cy Cuts at: 3243 3243 Size: 5992 EcoRI G'AATT_C Cuts at: 2990 3301 3679 2990 Size: 311 378 5303 Fragments arranged by size: 5303 378 311 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3086 3086 Size: 5992 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 5139 1678 Size: 3461 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2680 2712 4633 4665 4707 Size: 32 135 32 1108 32 1921 32 42 Cuts at: 4707 4739 1373 Size: 32 2626 Fragments arranged by size: 2626 1921 1108 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2101 2292 2302 3336 3582 669 Size: 988 281 163 191 10 1034 246 3079 Fragments arranged by size: 3079 1034 988 281 246 191 163 10 HindIII A'AGCT_T Cuts at: 748 2906 3283 748 Size: 2158 377 3457 Fragments arranged by size: 3457 2158 377 HpaI GTT'AAC Cuts at: 2292 3582 2292 Size: 1290 4702 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5106 5290 1305 Size: 3801 184 2007 Fragments arranged by size: 3801 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2064 10 Size: 54 1425 343 232 3938 Fragments arranged by size: 3938 1425 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2174 3950 4309 4468 518 Size: 648 843 165 1776 359 159 2042 Fragments arranged by size: 2042 1776 843 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 2095 3206 3408 4085 5233 5478 1606 Size: 489 1111 202 677 1148 245 2120 Fragments arranged by size: 2120 1148 1111 677 489 245 202 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4501 Fragments arranged by size: 4501 939 552 NdeI CA'TA_TG Cuts at: 387 2971 387 Size: 2584 3408 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 3350 4895 1132 Size: 97 2121 1545 2229 Fragments arranged by size: 2229 2121 1545 97 PasI CC'CwG_GG Cuts at: 1348 2772 1348 Size: 1424 4568 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2906 2938 4061 4093 5574 5606 2906 Size: 32 1123 32 1481 32 3292 Fragments arranged by size: 3292 1481 1123 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PspOMI G'GGCC_C Cuts at: 3243 3243 Size: 5992 PstI C_TGCA'G Cuts at: 830 3009 3344 830 Size: 2179 335 3478 Fragments arranged by size: 3478 2179 335 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 2095 2095 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SapI GCTCTTCn'nnn_ Cuts at: 2031 2031 Size: 5992 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SexAI A'CCwGG_T Cuts at: 2230 2230 Size: 5992 SfcI C'TryA_G Cuts at: 826 1045 3005 3218 3340 4475 5156 5347 826 Size: 219 1960 213 122 1135 681 191 1471 Fragments arranged by size: 1960 1471 1135 681 219 213 191 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2115 3071 3167 4096 4997 5259 Size: 197 926 172 956 96 929 901 262 Cuts at: 5259 820 Size: 1553 Fragments arranged by size: 1553 956 929 926 901 262 197 172 96 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 2384 1517 Size: 867 5125 StyI C'CwwG_G Cuts at: 513 1065 2004 3431 513 Size: 552 939 1427 3074 Fragments arranged by size: 3074 1427 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4019 4036 4189 4374 4819 325 Size: 1255 2439 17 153 185 445 1498 Fragments arranged by size: 2439 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4238 5950 96 Size: 274 80 33 51 1443 2261 1712 138 Fragments arranged by size: 2261 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5952 5984 5952 Size: 32 5960 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2515 2515 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 2040 2990 3185 4121 2040 Size: 950 195 936 3911 Fragments arranged by size: 3911 950 936 195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI