(Circular) (Six-base) MAPSORT of: pFN21AB9185.seq Check: 8815 from: 1 to: 5833 pFN21AB9185 5833 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2055 2622 2055 Size: 567 5266 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 2683 2956 3896 5280 5548 1632 Size: 413 638 273 940 1384 268 1917 Fragments arranged by size: 1917 1384 940 638 413 273 268 AfeI AGC'GCT Cuts at: 2255 2336 2255 Size: 81 5752 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 2508 2760 4732 5709 1203 Size: 1305 252 1972 977 1327 Fragments arranged by size: 1972 1327 1305 977 252 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2668 1184 Size: 1484 4349 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 2924 2956 2414 Size: 32 478 32 5291 Fragments arranged by size: 5291 478 32 32 AlwNI CAG_nnn'CTG Cuts at: 3576 5148 3576 Size: 1572 4261 ApaLI G'TGCA_C Cuts at: 2104 3890 5046 2104 Size: 1786 1156 2891 Fragments arranged by size: 2891 1786 1156 ApoI r'AATT_y Cuts at: 1493 3142 3368 3520 5629 1493 Size: 1649 226 152 2109 1697 Fragments arranged by size: 2109 1697 1649 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 2501 3158 1870 Size: 73 558 657 4545 Fragments arranged by size: 4545 657 558 73 BamHI G'GATC_C Cuts at: 2730 3163 3530 2730 Size: 433 367 5033 Fragments arranged by size: 5033 433 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2484 3154 4609 618 Size: 325 217 627 697 670 1455 1842 Fragments arranged by size: 1842 1455 697 670 627 325 217 BanII G_rGCy'C Cuts at: 1391 2357 3152 1391 Size: 966 795 4072 Fragments arranged by size: 4072 966 795 BbeI G_GCGC'C Cuts at: 2488 2488 Size: 5833 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2033 2056 2793 928 Size: 971 134 23 737 3968 Fragments arranged by size: 3968 971 737 134 23 BbvCI CC'TCA_GC Cuts at: 2480 2480 Size: 5833 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4024 4058 1903 Size: 34 2087 34 3678 Fragments arranged by size: 3678 2087 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 2108 2394 3894 5050 1790 Size: 318 286 1500 1156 2573 Fragments arranged by size: 2573 1500 1156 318 286 BmgBI CAC'GTC Cuts at: 1318 2409 1318 Size: 1091 4742 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 3564 4522 Size: 416 53 83 399 24 609 1538 958 Cuts at: 4522 442 Size: 1753 Fragments arranged by size: 1753 1538 958 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2796 2796 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2197 2219 2441 2840 3082 4493 1239 Size: 958 22 222 399 242 1411 2579 Fragments arranged by size: 2579 1411 958 399 242 222 22 Bpu10I CC'TnA_GC Cuts at: 2480 2586 2480 Size: 106 5727 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2588 3958 4823 5121 2588 Size: 1370 865 298 3300 Fragments arranged by size: 3300 1370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2543 4496 882 Size: 617 1044 1953 2219 Fragments arranged by size: 2219 1953 1044 617 BsaAI yAC'GTr Cuts at: 493 1204 2389 3624 493 Size: 711 1185 1235 2702 Fragments arranged by size: 2702 1235 1185 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2485 2988 3640 4022 Size: 53 83 186 936 952 503 652 382 Cuts at: 4022 4657 275 Size: 635 1451 Fragments arranged by size: 1451 952 936 652 635 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 2414 2444 1178 Size: 30 165 30 789 30 192 30 4567 Fragments arranged by size: 4567 789 192 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 2785 1186 Size: 1279 320 4234 Fragments arranged by size: 4234 1279 320 BseYI C'CCAG_C Cuts at: 2347 2725 5036 5532 2347 Size: 378 2311 496 2648 Fragments arranged by size: 2648 2311 496 378 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2027 2197 1163 Size: 573 291 170 4799 Fragments arranged by size: 4799 573 291 170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2108 2271 2357 3894 3979 5050 2108 Size: 163 86 1537 85 1071 2891 Fragments arranged by size: 2891 1537 1071 163 86 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2271 2357 2394 3152 3894 3979 Size: 399 318 163 86 37 758 742 85 Cuts at: 3979 5050 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 758 742 399 318 163 86 85 37 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 2622 3188 844 Size: 1211 567 566 3489 Fragments arranged by size: 3489 1211 567 566 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2122 4477 4664 1700 Size: 110 312 2355 187 2869 Fragments arranged by size: 2869 2355 312 187 110 BsrGI T'GTAC_A Cuts at: 96 2222 96 Size: 2126 3707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5833 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2730 3163 3530 3915 3932 5384 5470 5482 Size: 758 433 367 385 17 1452 86 12 Cuts at: 5482 5828 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 758 433 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2244 2466 2621 2918 513 Size: 552 939 240 222 155 297 3428 Fragments arranged by size: 3428 939 552 297 240 222 155 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DraIII CAC_nnn'GTG Cuts at: 2220 2220 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2468 4840 809 Size: 507 1152 2372 1802 Fragments arranged by size: 2372 1802 1152 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2120 3280 4169 Size: 54 1425 50 275 18 290 1160 889 Cuts at: 4169 5798 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1160 889 290 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2975 3763 1353 Size: 1622 788 3423 Fragments arranged by size: 3423 1622 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2771 4420 4794 4940 387 Size: 1064 138 1182 1649 374 146 1280 Fragments arranged by size: 1649 1280 1182 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2355 2355 Size: 5833 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2197 2219 2441 2683 2840 2956 Size: 393 413 152 22 222 242 157 116 Cuts at: 2956 3082 3896 4493 5280 5548 1239 Size: 126 814 597 787 268 1524 Fragments arranged by size: 1524 814 787 597 413 393 268 242 222 157 152 126 116 22 EcoO109I rG'GnC_Cy Cuts at: 2362 2362 Size: 5833 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 2117 2257 2338 2488 4980 1678 Size: 439 140 81 150 2492 2531 Fragments arranged by size: 2531 2492 439 150 140 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2854 2886 2994 Size: 32 135 32 620 32 630 32 108 Cuts at: 2994 3026 4474 4506 4548 4580 1373 Size: 32 1448 32 42 32 2626 Fragments arranged by size: 2626 1448 630 620 135 108 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KasI G'GCGC_C Cuts at: 2484 2484 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2472 4947 5131 1305 Size: 1167 2475 184 2007 Fragments arranged by size: 2475 2007 1167 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2573 2765 3791 4150 4309 518 Size: 648 1407 192 1026 359 159 2042 Fragments arranged by size: 2042 1407 1026 648 359 192 159 MspA1I CmG'CkG Cuts at: 1606 2101 2623 2675 2797 3000 3249 3926 5074 Size: 495 522 52 122 203 249 677 1148 Cuts at: 5074 5319 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 522 495 249 245 203 122 52 NarI GG'CG_CC Cuts at: 2485 2485 Size: 5833 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4342 Fragments arranged by size: 4342 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 2512 2764 2884 3191 4736 1132 Size: 97 1283 252 120 307 1545 2229 Fragments arranged by size: 2229 1545 1283 307 252 120 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 2508 2760 4732 2508 Size: 252 1972 3609 Fragments arranged by size: 3609 1972 252 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PfoI T'CCnGG_A Cuts at: 2856 2856 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2414 2446 3902 3934 5415 5447 2414 Size: 32 1456 32 1481 32 2800 Fragments arranged by size: 2800 1481 1456 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2362 Size: 5833 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 3185 830 Size: 2355 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2101 2675 2101 Size: 574 5259 SacI G_AGCT'C Cuts at: 2357 2357 Size: 5833 SacII CC_GC'GG Cuts at: 2624 2624 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 3181 4316 4997 5188 826 Size: 219 2136 1135 681 191 1471 Fragments arranged by size: 2136 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2486 2486 Size: 5833 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2603 3937 4838 5100 820 Size: 197 926 660 1334 901 262 1553 Fragments arranged by size: 1553 1334 926 901 660 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 2628 3272 513 Size: 552 939 354 270 644 3074 Fragments arranged by size: 3074 939 644 552 354 270 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2307 3860 3877 4030 4215 4660 325 Size: 1255 727 1553 17 153 185 445 1498 Fragments arranged by size: 1553 1498 1255 727 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2222 2505 2523 Size: 274 80 33 51 1443 245 283 18 Cuts at: 2523 4079 5791 96 Size: 1556 1712 138 Fragments arranged by size: 1712 1556 1443 283 274 245 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2314 2346 5793 5825 2314 Size: 32 3447 32 2322 Fragments arranged by size: 3447 2322 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI