Restriction map for
pFN21AB5447
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 362 CGCG CG^CG
AfaI 369 GTAC GT^AC
AgeI 644 ACCGGT A^CCGGT
AhdI 463 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 463 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 81 GGATCC G^GATCC
BanI 3 GGCACC G^G[CT][GA]CC
BclI 1065 TGATCA T^GATCA
BglII 1003 AGATCT A^GATCT
BsaAI 1198 TACGTG [CT]AC^GT[GA]
BsmFI 1045 GGGAC GGGAC(10/14)
BsmI 537 GAATGC GAATGC(1/-1)
BspHI 635 TCATGA T^CATGA
BsrFI 644 ACCGGT [GA]^CCGG[CT]
BssSI 205 CACGAG CACGAG(-5/-1)
BstBI 338 TTCGAA TT^CGAA
BstUI 362 CGCG CG^CG
CfoI 361 GCGC GCG^C
Cfr10I 644 ACCGGT [GA]^CCGG[CT]
DraI 457 TTTAAA TTT^AAA
DraII 1045 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 463 GACATTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 495 GAAGAG CTCTTC(1/4)
EcoO109I 1045 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 264 CCTTGG C^C[AT][AT]GG
FbaI 1065 TGATCA T^GATCA
HhaI 361 GCGC GCG^C
HinP1I 361 GCGC G^CGC
HincII 100 GTCAAC GT[CT]^[GA]AC
HindII 100 GTCAAC GT[CT]^[GA]AC
Ksp632I 495 GAAGAG CTCTTC(1/4)
MaeIII 1225 GTCAC ^GT[ACGT]AC
MslI 568 CACGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 446 CCGCTG C[AC]G^C[GT]G
MvnI 362 CGCG CG^CG
NspI 1076 GCATGT [GA]CATG^[CT]
NspV 338 TTCGAA TT^CGAA
PinAI 644 ACCGGT A^CCGGT
PpuMI 1045 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 635 TCATGA T^CATGA
RsaI 369 GTAC GT^AC
SacI 943 GAGCTC GAGCT^C
SfuI 338 TTCGAA TT^CGAA
SmlI 286 CTCAAG C^T[CT][GA]AG
StyI 264 CCTTGG C^C[AT][AT]GG
Tsp45I 1225 GTCAC ^GT[GC]AC
XbaI 1191 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 332 GTGCAC G^TGCAC
575 GTGCAC
AlwI 82 GATCC GGATC(4/5)
81 GGATC
ApaLI 332 GTGCAC G^TGCAC
575 GTGCAC
BalI 920 TGGCCA TGG^CCA
1202 TGGCCA
BanII 784 GGGCTC G[GA]GC[CT]^C
943 GAGCTC
BbsI 972 GAAGAC GAAGAC(2/6)
52 GTCTTC
BbvI 448 GCTGC GCAGC(8/12)
1146 GCTGC
BfaI 1024 CTAG C^TAG
1192 CTAG
BpuAI 972 GAAGAC GAAGAC(2/6)
52 GTCTTC
BspMI 1142 ACCTGC ACCTGC(4/8)
1107 GCAGGT
BstYI 81 GGATCC [GA]^GATC[CT]
1003 AGATCT
Cac8I 33 GCAGGC GC[ACGT]^[ACGT]GC
922 GCCAGC
EaeI 920 TGGCCA [CT]^GGCC[GA]
1202 TGGCCA
Fnu4HI 448 GCTGC GC^[ACGT]GC
1146 GCTGC
HphI 412 TCACC GGTGA(8/7)
1226 TCACC
ItaI 448 GCTGC GC^[ACGT]GC
1146 GCTGC
MaeI 1024 CTAG C^TAG
1192 CTAG
MaeII 1166 ACGT A^CGT
1199 ACGT
MflI 81 GGATCC [GA]^GATC[CT]
1003 AGATCT
MluNI 920 TGGCCA TGG^CCA
1202 TGGCCA
MscI 920 TGGCCA TGG^CCA
1202 TGGCCA
MseI 458 TTAA T^TAA
1177 TTAA
PleI 667 GACTC GAGTC(4/5)
800 GAGTC
Tru9I 458 TTAA T^TAA
1177 TTAA
TseI 448 GCTGC G^C[AT]GC
1146 GCTGC
XhoII 81 GGATCC [GA]^GATC[CT]
1003 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccIII AciI AluI AspHI AvaII
BmyI BpmI BsaJI BsaWI BseAI
BsiHKAI BsiYI BslI Bsp1286I BspEI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII FauI FokI
HaeIII HapII HinfI HpaII MboI
MboII MnlI MroI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tsp509I TspRI TthHB8I