(Circular) (Six-base) MAPSORT of: pFN21AB5447.seq Check: 708 from: 1 to: 5944 pFN21AB5447 5944 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3754 278 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3287 1937 Size: 1350 4594 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 4071 4444 1563 Size: 2508 373 3063 Fragments arranged by size: 3063 2508 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2062 2763 4007 5391 5659 1632 Size: 430 701 1244 1384 268 1917 Fragments arranged by size: 1917 1384 1244 701 430 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 4843 5820 1203 Size: 3640 977 1327 Fragments arranged by size: 3640 1327 977 AgeI A'CCGG_T Cuts at: 2649 4775 2649 Size: 2126 3818 AhdI GACnn_n'nnGTC Cuts at: 2473 4673 2473 Size: 2200 3744 AleI CACnn'nnGTG Cuts at: 1166 2577 1166 Size: 1411 4533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2202 1184 Size: 1018 4926 AlwNI CAG_nnn'CTG Cuts at: 3687 5259 3687 Size: 1572 4372 ApaLI G'TGCA_C Cuts at: 2337 2580 4001 5157 2337 Size: 243 1421 1156 3124 Fragments arranged by size: 3124 1421 1156 243 ApoI r'AATT_y Cuts at: 1493 3253 3479 3631 5740 1493 Size: 1760 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4498 160 Size: 872 3466 1606 Fragments arranged by size: 3466 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 3269 1870 Size: 73 1326 4545 Fragments arranged by size: 4545 1326 73 BamHI G'GATC_C Cuts at: 2086 3274 3641 2086 Size: 1188 367 4389 Fragments arranged by size: 4389 1188 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 3265 4720 618 Size: 325 217 627 221 1257 1455 1842 Fragments arranged by size: 1842 1455 1257 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2793 2952 3263 1391 Size: 1402 159 311 4072 Fragments arranged by size: 4072 1402 311 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2050 2984 928 Size: 971 151 934 3888 Fragments arranged by size: 3888 971 934 151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4135 4169 1903 Size: 34 2198 34 3678 Fragments arranged by size: 3678 2198 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 3070 1548 Size: 1522 4422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4555 136 Size: 107 122 71 631 605 2883 1525 Fragments arranged by size: 2883 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3008 5939 3008 Size: 2931 3013 BlpI GC'TnA_GC Cuts at: 3361 3361 Size: 5944 Bme1580I G_kGCm'C Cuts at: 1790 2341 2584 2767 3025 4005 5161 1790 Size: 551 243 183 258 980 1156 2573 Fragments arranged by size: 2573 1156 980 551 258 243 183 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5944 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2497 2604 2761 Size: 416 53 83 399 24 1080 107 157 Cuts at: 2761 3105 3675 4633 442 Size: 344 570 958 1753 Fragments arranged by size: 1753 1080 958 570 416 399 344 157 107 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2558 2726 4604 1239 Size: 1262 57 168 1878 2579 Fragments arranged by size: 2579 1878 1262 168 57 Bpu10I CC'TnA_GC Cuts at: 2946 2946 Size: 5944 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2276 4069 4934 5232 2276 Size: 1793 865 298 2988 Fragments arranged by size: 2988 1793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4607 882 Size: 617 3108 2219 Fragments arranged by size: 3108 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3205 3735 493 Size: 711 2001 530 2702 Fragments arranged by size: 2702 2001 711 530 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3751 4133 4768 275 Size: 53 83 186 936 2218 382 635 1451 Fragments arranged by size: 2218 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2089 2244 2611 2649 2991 4377 4775 5049 5196 Size: 155 367 38 342 1386 398 274 147 Cuts at: 5196 5607 2089 Size: 411 2426 Fragments arranged by size: 2426 1386 411 398 367 342 274 155 147 38 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5719 Fragments arranged by size: 5719 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5944 BseYI C'CCAG_C Cuts at: 2043 2284 5147 5643 2043 Size: 241 2863 496 2344 Fragments arranged by size: 2863 2344 496 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5371 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 4155 5183 1540 Size: 275 186 1393 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2341 2584 2917 2952 4005 4090 5161 2341 Size: 243 333 35 1053 85 1071 3124 Fragments arranged by size: 3124 1071 1053 333 243 85 35 BsmI GAATG_Cn' Cuts at: 2548 3455 3548 2548 Size: 907 93 4944 Fragments arranged by size: 4944 907 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2341 2584 2767 2793 2917 2952 3025 Size: 399 551 243 183 26 124 35 73 Cuts at: 3025 3263 4005 4090 5161 1391 Size: 238 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 551 399 243 238 183 124 85 73 35 26 BspEI T'CCGG_A Cuts at: 2089 2244 2611 5607 2089 Size: 155 367 2996 2426 Fragments arranged by size: 2996 2426 367 155 BspHI T'CATG_A Cuts at: 2640 3833 5563 2640 Size: 1193 1730 3021 Fragments arranged by size: 3021 1730 1193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3103 3156 3299 844 Size: 2259 53 143 3489 Fragments arranged by size: 3489 2259 143 53 BsrBI CCG'CTC Cuts at: 1394 1823 3831 1394 Size: 429 2008 3507 Fragments arranged by size: 3507 2008 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4439 4613 65 Size: 1183 3191 174 1396 Fragments arranged by size: 3191 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2649 4588 4775 1700 Size: 110 839 1939 187 2869 Fragments arranged by size: 2869 1939 839 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 2210 4004 5016 2210 Size: 1794 1012 3138 Fragments arranged by size: 3138 1794 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5944 BstBI TT'CG_AA Cuts at: 2344 4748 2344 Size: 2404 3540 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5944 BstYI r'GATC_y Cuts at: 1972 2086 3008 3274 3641 4026 4043 5495 5581 Size: 114 922 266 367 385 17 1452 86 Cuts at: 5581 5593 5939 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 922 385 367 346 266 114 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4453 Fragments arranged by size: 4453 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5205 Fragments arranged by size: 5205 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2624 3460 4254 4274 2067 Size: 557 836 794 20 3737 Fragments arranged by size: 3737 836 794 557 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3157 3192 529 Size: 35 2593 35 3281 Fragments arranged by size: 3281 2593 35 35 DraI TTT'AAA Cuts at: 2464 3248 2464 Size: 784 5160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4951 809 Size: 507 3635 1802 Fragments arranged by size: 3635 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2925 3207 3391 Size: 54 1425 50 275 18 1095 282 184 Cuts at: 3391 4280 5909 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1095 889 282 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2495 3874 1353 Size: 1142 1379 3423 Fragments arranged by size: 3423 1379 1142 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4531 4905 5051 387 Size: 1064 138 2942 374 146 1280 Fragments arranged by size: 2942 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2950 2950 Size: 5944 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2501 2558 2726 2763 4007 4604 Size: 393 430 439 57 168 37 1244 597 Cuts at: 4604 5391 5659 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1244 787 597 439 430 393 268 168 57 37 EcoO109I rG'GnC_Cy Cuts at: 3051 3051 Size: 5944 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FspI TGC'GCA Cuts at: 4450 4450 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 5091 1678 Size: 3413 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 2499 2531 3000 Size: 32 135 32 887 32 8 32 469 Cuts at: 3000 3032 4585 4617 4659 4691 1373 Size: 32 1553 32 42 32 2626 Fragments arranged by size: 2626 1553 887 469 135 42 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 2107 3288 3534 669 Size: 988 281 169 1181 246 3079 Fragments arranged by size: 3079 1181 988 281 246 169 HindIII A'AGCT_T Cuts at: 748 748 Size: 5944 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 3543 3543 Size: 5944 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2806 5058 5242 1305 Size: 1501 2252 184 2007 Fragments arranged by size: 2252 2007 1501 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2927 3209 10 Size: 54 1425 343 1095 282 2745 Fragments arranged by size: 2745 1425 1095 343 282 54 MslI CAynn'nnrTG Cuts at: 518 1166 2577 3902 4261 4420 518 Size: 648 1411 1325 359 159 2042 Fragments arranged by size: 2042 1411 1325 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2453 3360 4037 5185 5430 1606 Size: 847 907 677 1148 245 2120 Fragments arranged by size: 2120 1148 907 847 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4453 Fragments arranged by size: 4453 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 3085 3302 4847 1132 Size: 97 1856 217 1545 2229 Fragments arranged by size: 2229 1856 1545 217 97 PasI CC'CwG_GG Cuts at: 1348 2148 2908 3047 1348 Size: 800 760 139 4245 Fragments arranged by size: 4245 800 760 139 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5944 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4045 5526 5558 4013 Size: 32 1481 32 4399 Fragments arranged by size: 4399 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3051 3051 Size: 5944 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5944 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PstI C_TGCA'G Cuts at: 830 3296 830 Size: 2466 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 SacI G_AGCT'C Cuts at: 2952 2952 Size: 5944 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 4192 5904 4192 Size: 1712 4232 SfcI C'TryA_G Cuts at: 826 1045 3292 4427 5108 5299 826 Size: 219 2247 1135 681 191 1471 Fragments arranged by size: 2247 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 3271 1872 Size: 1399 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2291 4048 4949 5211 820 Size: 197 926 348 1757 901 262 1553 Fragments arranged by size: 1757 1553 926 901 348 262 197 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5944 StyI C'CwwG_G Cuts at: 513 1065 2004 2269 3383 513 Size: 552 939 265 1114 3074 Fragments arranged by size: 3074 1114 939 552 265 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2463 3971 3988 4141 4326 4771 325 Size: 1255 883 1508 17 153 185 445 1498 Fragments arranged by size: 1508 1498 1255 883 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4190 5902 96 Size: 274 80 33 51 1443 2213 1712 138 Fragments arranged by size: 2213 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5904 5936 5904 Size: 32 5912 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5944 XbaI T'CTAG_A Cuts at: 3196 3280 3196 Size: 84 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 3269 1870 Size: 1399 4545 XmnI GAAnn'nnTTC Cuts at: 4073 4073 Size: 5944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3752 276 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI