Restriction map for
pFN21AB5447
(Linear) (Six-base) MAPSORT of: KIBB5447.seq Check: 6118 from: 1 to: 1239
KIBB5447.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 758 1239
Size: 57 701 481
Fragments arranged by size:
701 481 57
AgeI A'CCGG_T
Cuts at: 0 644 1239
Size: 644 595
AhdI GACnn_n'nnGTC
Cuts at: 0 468 1239
Size: 468 771
AleI CACnn'nnGTG
Cuts at: 0 572 1239
Size: 572 667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 197 1239
Size: 197 1042
ApaLI G'TGCA_C
Cuts at: 0 332 575 1239
Size: 332 243 664
Fragments arranged by size:
664 332 243
BamHI G'GATC_C
Cuts at: 0 81 1239
Size: 81 1158
BanI G'GyrC_C
Cuts at: 0 3 1239
Size: 3 1236
BanII G_rGCy'C
Cuts at: 0 788 947 1239
Size: 788 159 292
Fragments arranged by size:
788 292 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 45 979 1239
Size: 45 934 260
Fragments arranged by size:
934 260 45
BclI T'GATC_A
Cuts at: 0 1065 1239
Size: 1065 174
BglII A'GATC_T
Cuts at: 0 1003 1239
Size: 1003 236
Bme1580I G_kGCm'C
Cuts at: 0 336 579 762 1020 1239
Size: 336 243 183 258 219
Fragments arranged by size:
336 258 243 219 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 492 599 756 1100 1239
Size: 492 107 157 344 139
Fragments arranged by size:
492 344 157 139 107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 553 721 1239
Size: 496 57 168 518
Fragments arranged by size:
518 496 168 57
Bpu10I CC'TnA_GC
Cuts at: 0 941 1239
Size: 941 298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 1239
Size: 271 968
BsaAI yAC'GTr
Cuts at: 0 1200 1239
Size: 1200 39
BsaWI w'CCGG_w
Cuts at: 0 84 239 606 644 986 1239
Size: 84 155 367 38 342 253
Fragments arranged by size:
367 342 253 155 84 38
BseYI C'CCAG_C
Cuts at: 0 38 279 1239
Size: 38 241 960
Fragments arranged by size:
960 241 38
BsiHKAI G_wGCw'C
Cuts at: 0 336 579 912 947 1239
Size: 336 243 333 35 292
Fragments arranged by size:
336 333 292 243 35
BsmI GAATG_Cn'
Cuts at: 0 543 1239
Size: 543 696
Bsp1286I G_dGCh'C
Cuts at: 0 336 579 762 788 912 947 1020 1239
Size: 336 243 183 26 124 35 73 219
Fragments arranged by size:
336 243 219 183 124 73 35 26
BspEI T'CCGG_A
Cuts at: 0 84 239 606 1239
Size: 84 155 367 633
Fragments arranged by size:
633 367 155 84
BspHI T'CATG_A
Cuts at: 0 635 1239
Size: 635 604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1098 1151 1239
Size: 1098 53 88
Fragments arranged by size:
1098 88 53
BsrFI r'CCGG_y
Cuts at: 0 644 1239
Size: 644 595
BssSI C'ACGA_G
Cuts at: 0 205 1239
Size: 205 1034
BstBI TT'CG_AA
Cuts at: 0 339 1239
Size: 339 900
BstYI r'GATC_y
Cuts at: 0 81 1003 1239
Size: 81 922 236
Fragments arranged by size:
922 236 81
BtsI GCAGTG_nn'
Cuts at: 0 62 619 1239
Size: 62 557 620
Fragments arranged by size:
620 557 62
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1152 1187 1239
Size: 1152 35 52
Fragments arranged by size:
1152 52 35
DraI TTT'AAA
Cuts at: 0 459 1239
Size: 459 780
EaeI y'GGCC_r
Cuts at: 0 920 1202 1239
Size: 920 282 37
Fragments arranged by size:
920 282 37
EarI CTCTTCn'nnn_
Cuts at: 0 490 1239
Size: 490 749
EcoICRI GAG'CTC
Cuts at: 0 945 1239
Size: 945 294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 496 553 721 758 1239
Size: 57 439 57 168 37 481
Fragments arranged by size:
481 439 168 57 57 37
EcoO109I rG'GnC_Cy
Cuts at: 0 1046 1239
Size: 1046 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 494 526 995 1027 1239
Size: 454 32 8 32 469 32 212
Fragments arranged by size:
469 454 212 32 32 32 8
HincII GTy'rAC
Cuts at: 0 102 1239
Size: 102 1137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 801 1239
Size: 801 438
MscI TGG'CCA
Cuts at: 0 922 1204 1239
Size: 922 282 35
Fragments arranged by size:
922 282 35
MslI CAynn'nnrTG
Cuts at: 0 572 1239
Size: 572 667
MspA1I CmG'CkG
Cuts at: 0 448 1239
Size: 448 791
NspI r_CATG'y
Cuts at: 0 1080 1239
Size: 1080 159
PasI CC'CwG_GG
Cuts at: 0 143 903 1042 1239
Size: 143 760 139 197
Fragments arranged by size:
760 197 143 139
PpuMI rG'GwC_Cy
Cuts at: 0 1046 1239
Size: 1046 193
SacI G_AGCT'C
Cuts at: 0 947 1239
Size: 947 292
SmlI C'TyrA_G
Cuts at: 0 286 1239
Size: 286 953
StyI C'CwwG_G
Cuts at: 0 264 1239
Size: 264 975
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 458 1239
Size: 458 781
XbaI T'CTAG_A
Cuts at: 0 1191 1239
Size: 1191 48
Enzymes that do cut:
AcuI AgeI AhdI AleI AlfI ApaLI BamHI BanI
BanII BbsI BclI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaWI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrFI BssSI BstBI BstYI BtsI CspCI
DraI EaeI EarI EcoICRI Eco57MI EcoO109I Hin4I HincII
MmeI MscI MslI MspA1I NspI PasI PpuMI SacI
SmlI StyI TaqII XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglI
BlpI BmgBI BmtI BplI BsaI BsaBI BsaHI BsaXI
BseRI BsgI BsiEI BsiWI BsmBI BsrBI BsrDI BsrGI
BssHII BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI