(Circular) (Six-base) MAPSORT of: pFN21AB5185.seq Check: 23 from: 1 to: 5899 pFN21AB5185 5899 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2839 2839 Size: 5899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2640 3242 1937 Size: 703 602 4594 Fragments arranged by size: 4594 703 602 Acc65I G'GTAC_C Cuts at: 2022 3220 2022 Size: 1198 4701 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 3158 3962 5346 5614 1632 Size: 629 897 804 1384 268 1917 Fragments arranged by size: 1917 1384 897 804 629 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 4798 5775 1203 Size: 3595 977 1327 Fragments arranged by size: 3595 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2336 3119 1184 Size: 1152 783 3964 Fragments arranged by size: 3964 1152 783 AlwNI CAG_nnn'CTG Cuts at: 2596 2625 2728 2872 3642 5214 2596 Size: 29 103 144 770 1572 3281 Fragments arranged by size: 3281 1572 770 144 103 29 ApaI G_GGCC'C Cuts at: 2703 3088 2703 Size: 385 5514 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 2785 3224 1870 Size: 73 842 439 4545 Fragments arranged by size: 4545 842 439 73 BamHI G'GATC_C Cuts at: 2289 3229 3596 2289 Size: 940 367 4592 Fragments arranged by size: 4592 940 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2022 2774 2846 2972 3031 Size: 325 217 627 235 752 72 126 59 Cuts at: 3031 3220 4675 618 Size: 189 1455 1842 Fragments arranged by size: 1842 1455 752 627 325 235 217 189 126 72 59 BanII G_rGCy'C Cuts at: 1391 2087 2703 3088 3218 1391 Size: 696 616 385 130 4072 Fragments arranged by size: 4072 696 616 385 130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4090 4124 1903 Size: 34 50 34 2069 34 3678 Fragments arranged by size: 3678 2069 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4510 136 Size: 107 122 71 631 605 2838 1525 Fragments arranged by size: 2838 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 2253 2703 2779 3088 3960 5116 1790 Size: 463 450 76 309 872 1156 2573 Fragments arranged by size: 2573 1156 872 463 450 309 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5899 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3630 4588 442 Size: 416 53 83 399 24 2213 958 1753 Fragments arranged by size: 2213 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2621 2654 2657 2705 3194 4559 1239 Size: 1382 33 3 48 489 1365 2579 Fragments arranged by size: 2579 1382 1365 489 48 33 3 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 2561 2823 4024 4889 5187 2525 Size: 36 262 1201 865 298 3237 Fragments arranged by size: 3237 1201 865 298 262 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4562 882 Size: 617 3063 2219 Fragments arranged by size: 3063 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3690 493 Size: 711 2486 2702 Fragments arranged by size: 2702 2486 711 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3706 4088 4723 275 Size: 53 83 186 936 2173 382 635 1451 Fragments arranged by size: 2173 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4332 4730 5004 5151 5562 4332 Size: 398 274 147 411 4669 Fragments arranged by size: 4669 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2421 2451 1178 Size: 30 165 30 1018 30 4626 Fragments arranged by size: 4626 1018 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5899 BseYI C'CCAG_C Cuts at: 2692 2696 2966 5102 5598 2692 Size: 4 270 2136 496 2993 Fragments arranged by size: 2993 2136 496 270 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2720 1163 Size: 573 984 4342 Fragments arranged by size: 4342 984 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3349 4110 5138 1540 Size: 275 186 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2673 3960 4045 5116 2673 Size: 1287 85 1071 3456 Fragments arranged by size: 3456 1287 1071 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2253 2673 2703 2779 3088 3218 Size: 399 297 166 420 30 76 309 130 Cuts at: 3218 3960 4045 5116 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 420 399 309 297 166 130 85 76 30 BspEI T'CCGG_A Cuts at: 5562 5562 Size: 5899 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2004 2839 3087 3254 844 Size: 1160 835 248 167 3489 Fragments arranged by size: 3489 1160 835 248 167 BsrBI CCG'CTC Cuts at: 1394 1823 2113 2263 3786 1394 Size: 429 290 150 1523 3507 Fragments arranged by size: 3507 1523 429 290 150 BsrDI GCAATG_nn' Cuts at: 65 1248 4394 4568 65 Size: 1183 3146 174 1396 Fragments arranged by size: 3146 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4543 4730 1700 Size: 110 2733 187 2869 Fragments arranged by size: 2869 2733 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5899 BssSI C'ACGA_G Cuts at: 2367 3959 4971 2367 Size: 1592 1012 3295 Fragments arranged by size: 3295 1592 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2774 794 Size: 1980 3919 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 2209 1184 Size: 1025 4874 BstXI CCAn_nnnn'nTGG Cuts at: 2309 3172 2309 Size: 863 5036 BstYI r'GATC_y Cuts at: 1972 2289 2356 2559 3229 3596 3981 3998 5450 Size: 317 67 203 670 367 385 17 1452 Cuts at: 5450 5536 5548 5894 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 670 385 367 346 317 203 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 3185 3185 Size: 5899 BtgI C'CryG_G Cuts at: 513 1065 2424 2802 513 Size: 552 1359 378 3610 Fragments arranged by size: 3610 1359 552 378 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 2664 3415 4209 4229 2664 Size: 751 794 20 4334 Fragments arranged by size: 4334 794 751 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DraIII CAC_nnn'GTG Cuts at: 3031 3031 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3066 3162 3346 Size: 54 1425 50 275 18 1236 96 184 Cuts at: 3346 4235 5864 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1236 889 275 184 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3346 1537 Size: 275 1534 4090 Fragments arranged by size: 4090 1534 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3010 3829 1353 Size: 1657 819 3423 Fragments arranged by size: 3423 1657 819 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2267 2891 4486 4860 5006 387 Size: 1064 138 678 624 1595 374 146 1280 Fragments arranged by size: 1595 1280 1064 678 624 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2621 2654 2657 2705 3158 3194 Size: 393 629 360 33 3 48 453 36 Cuts at: 3194 3962 4559 5346 5614 1239 Size: 768 597 787 268 1524 Fragments arranged by size: 1524 787 768 629 597 453 393 360 268 48 36 33 3 EcoNI CCTnn'n_nnAGG Cuts at: 2347 2912 2347 Size: 565 5334 EcoO109I rG'GnC_Cy Cuts at: 2306 2455 2533 2622 2917 2937 3085 3117 3189 Size: 149 78 89 295 20 148 32 72 Cuts at: 3189 2306 Size: 5016 Fragments arranged by size: 5016 295 149 148 89 78 72 32 20 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2116 2116 Size: 5899 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 2033 2985 5046 1678 Size: 355 952 2061 2531 Fragments arranged by size: 2531 2061 952 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2341 2373 2421 2453 4540 Size: 32 135 32 769 32 48 32 2087 Cuts at: 4540 4572 4614 4646 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2087 769 135 48 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3243 3489 669 Size: 988 281 1305 246 3079 Fragments arranged by size: 3079 1305 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KpnI G_GTAC'C Cuts at: 2026 3224 2026 Size: 1198 4701 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5013 5197 1305 Size: 3708 184 2007 Fragments arranged by size: 3708 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3164 10 Size: 54 1425 343 1332 2745 Fragments arranged by size: 2745 1425 1332 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2504 3857 4216 4375 518 Size: 648 1338 1353 359 159 2042 Fragments arranged by size: 2042 1353 1338 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2551 2696 2728 2970 3315 3992 5140 5385 Size: 945 145 32 242 345 677 1148 245 Cuts at: 5385 1606 Size: 2120 Fragments arranged by size: 2120 1148 945 677 345 245 242 145 32 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5347 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 3257 4802 1132 Size: 97 2028 1545 2229 Fragments arranged by size: 2229 2028 1545 97 PasI CC'CwG_GG Cuts at: 1348 2038 2302 3112 1348 Size: 690 264 810 4135 Fragments arranged by size: 4135 810 690 264 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2625 2728 1108 Size: 1517 103 4279 Fragments arranged by size: 4279 1517 103 PfoI T'CCnGG_A Cuts at: 2991 2991 Size: 5899 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2306 2455 2306 Size: 149 5750 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2699 3084 2699 Size: 385 5514 PstI C_TGCA'G Cuts at: 830 3251 830 Size: 2421 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 PvuII CAG'CTG Cuts at: 2696 2728 2696 Size: 32 5867 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SanDI GG'GwC_CC Cuts at: 2455 2455 Size: 5899 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SfcI C'TryA_G Cuts at: 826 1045 2897 3003 3247 4382 5063 5254 826 Size: 219 1852 106 244 1135 681 191 1471 Fragments arranged by size: 1852 1471 1135 681 244 219 191 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 2787 3226 1872 Size: 915 439 4545 Fragments arranged by size: 4545 915 439 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 2576 2838 4003 4904 5166 Size: 197 926 597 36 262 1165 901 262 Cuts at: 5166 820 Size: 1553 Fragments arranged by size: 1553 1165 926 901 597 262 262 197 36 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5899 StyI C'CwwG_G Cuts at: 513 1065 2529 3338 513 Size: 552 1464 809 3074 Fragments arranged by size: 3074 1464 809 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 2990 3926 3943 4096 4281 4726 Size: 1255 450 960 936 17 153 185 445 Cuts at: 4726 325 Size: 1498 Fragments arranged by size: 1498 1255 960 936 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4145 5857 96 Size: 274 80 33 51 1443 2168 1712 138 Fragments arranged by size: 2168 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3015 3047 5859 5891 3015 Size: 32 2812 32 3023 Fragments arranged by size: 3023 2812 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 2785 3224 1870 Size: 915 439 4545 Fragments arranged by size: 4545 915 439 XmnI GAAnn'nnTTC Cuts at: 4028 4028 Size: 5899 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI