Restriction map for
pFN21AB5045
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflIII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BcgI
BciVI BclI BglI BlnI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrDI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 64 GTCTAC GT^[AC][GT]AC
AflII 1114 CTTAAG C^TTAAG
AlwNI 1250 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1396 CCTGAGG CC^T[ACGT]AGG
Asp700I 681 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1076 CTCGAG C^[CT]CG[GA]G
BalI 365 TGGCCA TGG^CCA
BbrPI 1237 CACGTG CAC^GTG
BfrI 1114 CTTAAG C^TTAAG
BglII 1388 AGATCT A^GATCT
BsaAI 1237 CACGTG [CT]AC^GT[GA]
BsaWI 1762 TCCGGT [AT]^CCGG[AT]
BseRI 661 GAGGAG GAGGAG(10/8)
BsoBI 1076 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1262 TGTACA T^GTACA
BsrBI 772 GAGCGG CCGCTC(-3/-3)
BsrFI 1452 GCCGGC [GA]^CCGG[CT]
BsrGI 1262 TGTACA T^GTACA
BstXI 1502 CCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1396 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1452 GCCGGC [GA]^CCGG[CT]
EarI 834 CTCTTC CTCTTC(1/4)
Eco81I 1396 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1269 ATGCAT ATGCA^T
HgaI 1069 GACGC GACGC(5/10)
Ksp632I 834 CTCTTC CTCTTC(1/4)
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
NaeI 1452 GCCGGC GCC^GGC
NcoI 714 CCATGG C^CATGG
NgoMIV 1452 GCCGGC G^CCGGC
NsiI 1269 ATGCAT ATGCA^T
PaeR7I 1076 CTCGAG C^TCGAG
PmaCI 1237 CACGTG CAC^GTG
PmlI 1237 CACGTG CAC^GTG
PstI 310 CTGCAG CTGCA^G
SexAI 872 ACCTGGT A^CC[AT]GGT
SspBI 1262 TGTACA T^GTACA
TfiI 87 GATTC G^A[AT]TC
XcmI 1364 CCACAGTAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1076 CTCGAG C^TCGAG
XmnI 681 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 233 GGTACC G^GTACC
869 GGTACC
Alw44I 25 GTGCAC G^TGCAC
180 GTGCAC
AlwI 267 GATCC GGATC(4/5)
1199 GGATC
ApaI 124 GGGCCC GGGCC^C
1844 GGGCCC
ApaLI 25 GTGCAC G^TGCAC
180 GTGCAC
Asp718I 233 GGTACC G^GTACC
869 GGTACC
BbvCI 116 CCTCAGC CCTCAGC(-5/-2)
864 GCTGAGG
BlpI 1173 GCTCAGC GC^T[ACGT]AGC
1330 GCTGAGC
Bpu1102I 1173 GCTCAGC GC^T[ACGT]AGC
1330 GCTGAGC
BsaI 1079 GAGACC GGTCTC(1/5)
1378 GAGACC
BsmAI 1079 GAGAC GTCTC(1/5)
1378 GAGAC
BsmFI 1165 GGGAC GGGAC(10/14)
205 GTCCC
BsmI 42 GAATGC GAATGC(1/-1)
777 GAATGC
BssSI 150 CTCGTG CACGAG(-5/-1)
1018 CTCGTG
BstEII 1275 GGTGACC G^GT[ACGT]ACC
1444 GGTTACC
BstPI 1275 GGTGACC G^GT[ACGT]ACC
1444 GGTTACC
BstYI 1199 GGATCT [GA]^GATC[CT]
1388 AGATCT
CelII 1173 GCTCAGC GC^T[ACGT]AGC
1330 GCTGAGC
EaeI 365 TGGCCA [CT]^GGCC[GA]
978 TGGCCG
EcoNI 195 CCTCTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1017 CCTCGTGGAGG
EcoO65I 1275 GGTGACC G^GT[ACGT]ACC
1444 GGTTACC
KpnI 233 GGTACC GGTAC^C
869 GGTACC
MaeII 1238 ACGT A^CGT
1435 ACGT
MflI 1199 GGATCT [GA]^GATC[CT]
1388 AGATCT
NheI 930 GCTAGC G^CTAGC
1614 GCTAGC
PflMI 294 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1834 CCAGCACATGG
PleI 1125 GACTC GAGTC(4/5)
1608 GACTC
PpuMI 203 AGGTCCC [GA]G^G[AT]CC[CT]
305 AGGTCCT
SfcI 310 CTGCAG C^T[GA][CT]AG
1350 CTACAG
StuI 700 AGGCCT AGG^CCT
1034 AGGCCT
Van91I 294 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1834 CCAGCACATGG
XhoII 1199 GGATCT [GA]^GATC[CT]
1388 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I