Restriction map for
pFN21AB5045
(Linear) (Six-base) MAPSORT of: KIBB5045.seq Check: 8251 from: 1 to: 1959
KIBB5045.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 979 1497 1959
Size: 979 518 462
Fragments arranged by size:
979 518 462
AccI GT'mk_AC
Cuts at: 0 65 1959
Size: 65 1894
Acc65I G'GTAC_C
Cuts at: 0 233 869 1959
Size: 233 636 1090
Fragments arranged by size:
1090 636 233
AflII C'TTAA_G
Cuts at: 0 1114 1959
Size: 1114 845
AleI CACnn'nnGTG
Cuts at: 0 1258 1959
Size: 1258 701
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 124 156 1959
Size: 124 32 1803
Fragments arranged by size:
1803 124 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1255 1959
Size: 1255 704
ApaI G_GGCC'C
Cuts at: 0 128 1848 1959
Size: 128 1720 111
Fragments arranged by size:
1720 128 111
ApaLI G'TGCA_C
Cuts at: 0 25 180 1959
Size: 25 155 1779
Fragments arranged by size:
1779 155 25
AvaI C'yCGr_G
Cuts at: 0 1076 1959
Size: 1076 883
BanI G'GyrC_C
Cuts at: 0 233 507 869 1219 1658 1959
Size: 233 274 362 350 439 301
Fragments arranged by size:
439 362 350 301 274 233
BanII G_rGCy'C
Cuts at: 0 128 455 542 727 1848 1959
Size: 128 327 87 185 1121 111
Fragments arranged by size:
1121 327 185 128 111 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 1576 1629 1724 1959
Size: 1576 53 95 235
Fragments arranged by size:
1576 235 95 53
BbvCI CC'TCA_GC
Cuts at: 0 117 865 1959
Size: 117 748 1094
Fragments arranged by size:
1094 748 117
BfrBI ATG'CAT
Cuts at: 0 1271 1959
Size: 1271 688
BglII A'GATC_T
Cuts at: 0 1388 1959
Size: 1388 571
BlpI GC'TnA_GC
Cuts at: 0 1174 1331 1959
Size: 1174 157 628
Fragments arranged by size:
1174 628 157
Bme1580I G_kGCm'C
Cuts at: 0 29 128 184 512 1502 1848 1959
Size: 29 99 56 328 990 346 111
Fragments arranged by size:
990 346 328 111 99 56 29
BmgBI CAC'GTC
Cuts at: 0 1436 1959
Size: 1436 523
BmrI ACTGGGnnnn_n'
Cuts at: 0 1265 1959
Size: 1265 694
BmtI G_CTAG'C
Cuts at: 0 934 1618 1959
Size: 934 684 341
Fragments arranged by size:
934 684 341
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 576 1959
Size: 576 1383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 403 571 679 688 1959
Size: 322 81 168 108 9 1271
Fragments arranged by size:
1271 322 168 108 81 9
Bpu10I CC'TnA_GC
Cuts at: 0 117 721 865 1959
Size: 117 604 144 1094
Fragments arranged by size:
1094 604 144 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 577 715 1959
Size: 526 51 138 1244
Fragments arranged by size:
1244 526 138 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 1073 1372 1959
Size: 1073 299 587
Fragments arranged by size:
1073 587 299
BsaAI yAC'GTr
Cuts at: 0 1239 1959
Size: 1239 720
BsaWI w'CCGG_w
Cuts at: 0 1762 1959
Size: 1762 197
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 1021 1051 1740 1770 1959
Size: 124 30 867 30 689 30 189
Fragments arranged by size:
867 689 189 124 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 676 1959
Size: 676 1283
BseYI C'CCAG_C
Cuts at: 0 1725 1959
Size: 1725 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 174 1864 1959
Size: 116 58 1690 95
Fragments arranged by size:
1690 116 95 58
BsiHKAI G_wGCw'C
Cuts at: 0 29 184 455 542 727 1051 1175 1327
Size: 29 155 271 87 185 324 124 152
Cuts at: 1327 1481 1959
Size: 154 478
Fragments arranged by size:
478 324 271 185 155 154 152 124
87 29
BsmI GAATG_Cn'
Cuts at: 0 48 783 1959
Size: 48 735 1176
Fragments arranged by size:
1176 735 48
Bsp1286I G_dGCh'C
Cuts at: 0 29 128 184 455 512 542 727 1051
Size: 29 99 56 271 57 30 185 324
Cuts at: 1051 1175 1327 1481 1502 1848 1959
Size: 124 152 154 21 346 111
Fragments arranged by size:
346 324 271 185 154 152 124 111
99 57 56 30 29 21
BspHI T'CATG_A
Cuts at: 0 746 1565 1803 1959
Size: 746 819 238 156
Fragments arranged by size:
819 746 238 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 6 979 1497 1959
Size: 6 973 518 462
Fragments arranged by size:
973 518 462 6
BsrBI CCG'CTC
Cuts at: 0 774 1959
Size: 774 1185
BsrFI r'CCGG_y
Cuts at: 0 1452 1959
Size: 1452 507
BsrGI T'GTAC_A
Cuts at: 0 1262 1959
Size: 1262 697
BssSI C'ACGA_G
Cuts at: 0 150 1018 1959
Size: 150 868 941
Fragments arranged by size:
941 868 150
BstEII G'GTnAC_C
Cuts at: 0 1275 1444 1959
Size: 1275 169 515
Fragments arranged by size:
1275 515 169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1509 1959
Size: 1509 450
BstYI r'GATC_y
Cuts at: 0 1199 1388 1959
Size: 1199 189 571
Fragments arranged by size:
1199 571 189
Bsu36I CC'TnA_GG
Cuts at: 0 1397 1959
Size: 1397 562
BtgI C'CryG_G
Cuts at: 0 714 1382 1472 1959
Size: 714 668 90 487
Fragments arranged by size:
714 668 487 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 87 1959
Size: 87 1872
BtsI GCAGTG_nn'
Cuts at: 0 1610 1959
Size: 1610 349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 883 918 1959
Size: 883 35 1041
Fragments arranged by size:
1041 883 35
EaeI y'GGCC_r
Cuts at: 0 365 978 1959
Size: 365 613 981
Fragments arranged by size:
981 613 365
EarI CTCTTCn'nnn_
Cuts at: 0 840 1959
Size: 840 1119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 274 1959
Size: 274 1685
EcoICRI GAG'CTC
Cuts at: 0 453 540 725 1959
Size: 453 87 185 1234
Fragments arranged by size:
1234 453 185 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 403 571 679 688 1959
Size: 322 81 168 108 9 1271
Fragments arranged by size:
1271 322 168 108 81 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 1021 1959
Size: 199 822 938
Fragments arranged by size:
938 822 199
EcoO109I rG'GnC_Cy
Cuts at: 0 125 192 204 306 350 422 1844 1959
Size: 125 67 12 102 44 72 1422 115
Fragments arranged by size:
1422 125 115 102 72 67 44 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1959
Size: 1021 32 906
Fragments arranged by size:
1021 906 32
KpnI G_GTAC'C
Cuts at: 0 237 873 1959
Size: 237 636 1086
Fragments arranged by size:
1086 636 237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 1102 1322 1370 1959
Size: 1081 21 220 48 589
Fragments arranged by size:
1081 589 220 48 21
MscI TGG'CCA
Cuts at: 0 367 1959
Size: 367 1592
MslI CAynn'nnrTG
Cuts at: 0 262 1000 1258 1270 1959
Size: 262 738 258 12 689
Fragments arranged by size:
738 689 262 258 12
MspA1I CmG'CkG
Cuts at: 0 864 983 1668 1959
Size: 864 119 685 291
Fragments arranged by size:
864 685 291 119
NaeI GCC'GGC
Cuts at: 0 1454 1959
Size: 1454 505
NcoI C'CATG_G
Cuts at: 0 714 1959
Size: 714 1245
NgoMIV G'CCGG_C
Cuts at: 0 1452 1959
Size: 1452 507
NheI G'CTAG_C
Cuts at: 0 930 1614 1959
Size: 930 684 345
Fragments arranged by size:
930 684 345
NsiI A_TGCA'T
Cuts at: 0 1273 1959
Size: 1273 686
NspI r_CATG'y
Cuts at: 0 615 633 1005 1271 1959
Size: 615 18 372 266 688
Fragments arranged by size:
688 615 372 266 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 300 1840 1959
Size: 300 1540 119
Fragments arranged by size:
1540 300 119
PmlI CAC'GTG
Cuts at: 0 1239 1959
Size: 1239 720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 568 600 1959
Size: 124 32 412 32 1359
Fragments arranged by size:
1359 412 124 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 204 306 1959
Size: 204 102 1653
Fragments arranged by size:
1653 204 102
PspOMI G'GGCC_C
Cuts at: 0 124 1844 1959
Size: 124 1720 115
Fragments arranged by size:
1720 124 115
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 217 249 1959
Size: 217 32 1710
Fragments arranged by size:
1710 217 32
PstI C_TGCA'G
Cuts at: 0 314 1959
Size: 314 1645
SacI G_AGCT'C
Cuts at: 0 455 542 727 1959
Size: 455 87 185 1232
Fragments arranged by size:
1232 455 185 87
SexAI A'CCwGG_T
Cuts at: 0 872 1959
Size: 872 1087
SfcI C'TryA_G
Cuts at: 0 310 1350 1959
Size: 310 1040 609
Fragments arranged by size:
1040 609 310
SmlI C'TyrA_G
Cuts at: 0 541 592 730 1076 1114 1959
Size: 541 51 138 346 38 845
Fragments arranged by size:
845 541 346 138 51 38
StuI AGG'CCT
Cuts at: 0 702 1036 1959
Size: 702 334 923
Fragments arranged by size:
923 702 334
StyI C'CwwG_G
Cuts at: 0 714 901 1195 1931 1959
Size: 714 187 294 736 28
Fragments arranged by size:
736 714 294 187 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1208 1959
Size: 1208 751
TatI w'GTAC_w
Cuts at: 0 1262 1415 1672 1959
Size: 1262 153 257 287
Fragments arranged by size:
1262 287 257 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 78 110 1959
Size: 78 32 1849
Fragments arranged by size:
1849 78 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1371 1959
Size: 1371 588
XhoI C'TCGA_G
Cuts at: 0 1076 1959
Size: 1076 883
XmnI GAAnn'nnTTC
Cuts at: 0 685 1959
Size: 685 1274
Enzymes that do cut:
AarI AccI Acc65I AflII AleI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BbvCI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NheI NsiI NspI PflMI PmlI PpiI PpuMI
PspOMI PsrI PstI SacI SexAI SfcI SmlI StuI
StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflIII AgeI AhdI AlfI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BglI BsaBI BsaHI BsiEI BsiWI
BsmBI BspEI BsrDI BssHII BstAPI BstBI BstZ17I ClaI
DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI NarI NdeI NotI NruI PacI PasI PciI
PfoI PmeI PshAI PsiI PspXI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XmaI ZraI