(Circular) (Six-base) MAPSORT of: pFN21AB3168.seq Check: 204 from: 1 to: 7075 pFN21AB3168 7075 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4885 278 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4418 1937 Size: 2481 4594 Acc65I G'GTAC_C Cuts at: 4396 4396 Size: 7075 AclI AA'CG_TT Cuts at: 1563 3493 5202 5575 1563 Size: 1930 1709 373 3063 Fragments arranged by size: 3063 1930 1709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2546 2813 5138 6522 6790 1632 Size: 914 267 2325 1384 268 1917 Fragments arranged by size: 2325 1917 1384 914 268 267 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6878 AflIII A'CryG_T Cuts at: 1203 2337 5974 6951 1203 Size: 1134 3637 977 1327 Fragments arranged by size: 3637 1327 1134 977 AgeI A'CCGG_T Cuts at: 5906 5906 Size: 7075 AhdI GACnn_n'nnGTC Cuts at: 5804 5804 Size: 7075 AleI CACnn'nnGTG Cuts at: 1166 4251 1166 Size: 3085 3990 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4130 1184 Size: 2946 4129 AlwNI CAG_nnn'CTG Cuts at: 2885 4818 6390 2885 Size: 1933 1572 3570 Fragments arranged by size: 3570 1933 1572 ApaI G_GGCC'C Cuts at: 3284 4238 4246 4297 3284 Size: 954 8 51 6062 Fragments arranged by size: 6062 954 51 8 ApaLI G'TGCA_C Cuts at: 5132 6288 5132 Size: 1156 5919 ApoI r'AATT_y Cuts at: 1493 2420 2456 2697 4384 4610 4762 6871 1493 Size: 927 36 241 1687 226 152 2109 1697 Fragments arranged by size: 2109 1697 1687 927 241 226 152 36 AseI AT'TA_AT Cuts at: 160 1032 5629 160 Size: 872 4597 1606 Fragments arranged by size: 4597 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 AvaI C'yCGr_G Cuts at: 1870 1943 3308 3318 3557 4155 4400 1870 Size: 73 1365 10 239 598 245 4545 Fragments arranged by size: 4545 1365 598 245 239 73 10 BamHI G'GATC_C Cuts at: 3553 4405 4772 3553 Size: 852 367 5856 Fragments arranged by size: 5856 852 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3992 4396 5851 618 Size: 325 217 627 2205 404 1455 1842 Fragments arranged by size: 2205 1842 1455 627 404 325 217 BanII G_rGCy'C Cuts at: 1391 3284 3294 3339 3446 3725 4200 4238 4246 Size: 1893 10 45 107 279 475 38 8 Cuts at: 4246 4297 4394 1391 Size: 51 97 4072 Fragments arranged by size: 4072 1893 475 279 107 97 51 45 38 10 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 2862 4275 928 Size: 971 192 771 1413 3728 Fragments arranged by size: 3728 1413 971 771 192 BbvCI CC'TCA_GC Cuts at: 3656 3656 Size: 7075 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3289 3323 3746 3780 5266 5300 1903 Size: 34 1352 34 423 34 1486 34 3678 Fragments arranged by size: 3678 1486 1352 423 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 4967 6177 4013 Size: 954 1210 4911 Fragments arranged by size: 4911 1210 954 BclI T'GATC_A Cuts at: 1548 4306 1548 Size: 2758 4317 BfrBI ATG'CAT Cuts at: 2320 2320 Size: 7075 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5686 136 Size: 107 122 71 631 605 4014 1525 Fragments arranged by size: 4014 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7070 7070 Size: 7075 BlpI GC'TnA_GC Cuts at: 4492 4492 Size: 7075 Bme1580I G_kGCm'C Cuts at: 1790 3108 3138 3284 4238 4246 4297 5136 6292 Size: 1318 30 146 954 8 51 839 1156 Cuts at: 6292 1790 Size: 2573 Fragments arranged by size: 2573 1318 1156 954 839 146 51 30 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7075 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4806 5764 Size: 416 53 83 399 24 1958 1431 958 Cuts at: 5764 442 Size: 1753 Fragments arranged by size: 1958 1753 1431 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7075 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3664 3664 Size: 7075 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2064 2456 3563 3634 3840 5735 1239 Size: 825 392 1107 71 206 1895 2579 Fragments arranged by size: 2579 1895 1107 825 392 206 71 Bpu10I CC'TnA_GC Cuts at: 3656 3656 Size: 7075 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2378 3765 4123 5200 6065 6363 2378 Size: 1387 358 1077 865 298 3090 Fragments arranged by size: 3090 1387 1077 865 358 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2832 3413 5738 882 Size: 617 1333 581 2325 2219 Fragments arranged by size: 2325 2219 1333 617 581 BsaAI yAC'GTr Cuts at: 493 1204 4866 493 Size: 711 3662 2702 Fragments arranged by size: 3662 2702 711 BsaBI GATnn'nnATC Cuts at: 2936 7069 2936 Size: 4133 2942 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3759 4882 5264 5899 Size: 53 83 186 936 2226 1123 382 635 Cuts at: 5899 275 Size: 1451 Fragments arranged by size: 2226 1451 1123 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 3717 5508 5906 6180 6327 6738 2271 Size: 1446 1791 398 274 147 411 2608 Fragments arranged by size: 2608 1791 1446 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6850 Fragments arranged by size: 6850 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3109 3371 3637 4208 1186 Size: 1923 262 266 571 4053 Fragments arranged by size: 4053 1923 571 266 262 BseYI C'CCAG_C Cuts at: 2952 3138 3204 3398 6278 6774 2952 Size: 186 66 194 2880 496 3253 Fragments arranged by size: 3253 2880 496 194 186 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3457 3491 3771 3844 1163 Size: 573 1721 34 280 73 4394 Fragments arranged by size: 4394 1721 573 280 73 34 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4525 5286 6314 1540 Size: 275 186 2524 761 1028 2301 Fragments arranged by size: 2524 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3227 4200 5136 5221 6292 3227 Size: 973 936 85 1071 4010 Fragments arranged by size: 4010 1071 973 936 85 BsmI GAATG_Cn' Cuts at: 2735 3733 3992 4352 4586 4679 2735 Size: 998 259 360 234 93 5131 Fragments arranged by size: 5131 998 360 259 234 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3108 3138 3227 3284 3294 3339 3446 Size: 399 1318 30 89 57 10 45 107 Cuts at: 3446 3725 4200 4238 4246 4297 4394 5136 5221 Size: 279 475 38 8 51 97 742 85 Cuts at: 5221 6292 1391 Size: 1071 2174 Fragments arranged by size: 2174 1318 1071 742 475 399 279 107 97 89 85 57 51 45 38 30 10 8 BspEI T'CCGG_A Cuts at: 2271 3717 6738 2271 Size: 1446 3021 2608 Fragments arranged by size: 3021 2608 1446 BspHI T'CATG_A Cuts at: 4063 4964 6694 4063 Size: 901 1730 4444 Fragments arranged by size: 4444 1730 901 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4430 844 Size: 3586 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4962 1394 Size: 429 3139 3507 Fragments arranged by size: 3507 3139 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4324 5570 5744 65 Size: 1183 3076 1246 174 1396 Fragments arranged by size: 3076 1396 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3833 5719 5906 1700 Size: 110 2023 1886 187 2869 Fragments arranged by size: 2869 2023 1886 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7075 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7075 BssSI C'ACGA_G Cuts at: 5135 6147 5135 Size: 1012 6063 BstAPI GCAn_nnn'nTGC Cuts at: 794 3618 4347 794 Size: 2824 729 3522 Fragments arranged by size: 3522 2824 729 BstBI TT'CG_AA Cuts at: 5879 5879 Size: 7075 BstEII G'GTnAC_C Cuts at: 1184 2785 1184 Size: 1601 5474 BstXI CCAn_nnnn'nTGG Cuts at: 3749 3749 Size: 7075 BstYI r'GATC_y Cuts at: 1972 2881 3553 3606 3700 4405 4772 5157 5174 Size: 909 672 53 94 705 367 385 17 Cuts at: 5174 6626 6712 6724 7070 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 909 705 672 385 367 346 94 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2025 3285 3354 3925 2025 Size: 1260 69 571 5175 Fragments arranged by size: 5175 1260 571 69 BtgI C'CryG_G Cuts at: 513 1065 2004 2544 2959 3120 513 Size: 552 939 540 415 161 4468 Fragments arranged by size: 4468 939 552 540 415 161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6336 Fragments arranged by size: 6336 683 56 BtsI GCAGTG_nn' Cuts at: 3423 4591 5385 5405 3423 Size: 1168 794 20 5093 Fragments arranged by size: 5093 1168 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7075 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7040 DraI TTT'AAA Cuts at: 2095 2125 2419 4379 2095 Size: 30 294 1960 4791 Fragments arranged by size: 4791 1960 294 30 DraIII CAC_nnn'GTG Cuts at: 3882 3882 Size: 7075 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6082 809 Size: 507 4766 1802 Fragments arranged by size: 4766 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4522 5411 7040 Size: 54 1425 50 275 18 2692 889 1629 Cuts at: 7040 8 Size: 43 Fragments arranged by size: 2692 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4522 1537 Size: 275 2710 4090 Fragments arranged by size: 4090 2710 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4077 5005 1353 Size: 2724 928 3423 Fragments arranged by size: 3423 2724 928 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5662 6036 6182 387 Size: 1064 138 4073 374 146 1280 Fragments arranged by size: 4073 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4198 4198 Size: 7075 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2456 2546 2813 3563 3634 3840 Size: 393 432 392 90 267 750 71 206 Cuts at: 3840 5138 5735 6522 6790 1239 Size: 1298 597 787 268 1524 Fragments arranged by size: 1524 1298 787 750 597 432 393 392 268 267 206 90 71 EcoNI CCTnn'n_nnAGG Cuts at: 2024 2024 Size: 7075 EcoO109I rG'GnC_Cy Cuts at: 3281 3312 3371 4235 4242 4294 3281 Size: 31 59 864 7 52 6062 Fragments arranged by size: 6062 864 59 52 31 7 EcoRI G'AATT_C Cuts at: 4384 4762 4384 Size: 378 6697 FspI TGC'GCA Cuts at: 5581 5581 Size: 7075 HaeII r_GCGC'y Cuts at: 1678 2288 2381 3308 6222 1678 Size: 610 93 927 2914 2531 Fragments arranged by size: 2914 2531 927 610 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2101 2133 5716 5748 5790 Size: 32 135 32 529 32 3583 32 42 Cuts at: 5790 5822 1373 Size: 32 2626 Fragments arranged by size: 3583 2626 529 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2833 4419 4665 669 Size: 988 281 895 1586 246 3079 Fragments arranged by size: 3079 1586 988 895 281 246 HindIII A'AGCT_T Cuts at: 748 2276 748 Size: 1528 5547 HpaI GTT'AAC Cuts at: 2833 4665 2833 Size: 1832 5243 KpnI G_GTAC'C Cuts at: 4400 4400 Size: 7075 MfeI C'AATT_G Cuts at: 2308 4674 2308 Size: 2366 4709 MluI A'CGCG_T Cuts at: 6951 6951 Size: 7075 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2973 6189 6373 1305 Size: 1668 3216 184 2007 Fragments arranged by size: 3216 2007 1668 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5253 Fragments arranged by size: 5253 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 3194 3428 4251 5033 5392 5551 Size: 648 1377 651 234 823 782 359 159 Cuts at: 5551 518 Size: 2042 Fragments arranged by size: 2042 1377 823 782 651 648 359 234 159 MspA1I CmG'CkG Cuts at: 1606 2427 4491 5168 6316 6561 1606 Size: 821 2064 677 1148 245 2120 Fragments arranged by size: 2120 2064 1148 821 677 245 NaeI GCC'GGC Cuts at: 3835 3835 Size: 7075 NcoI C'CATG_G Cuts at: 513 1065 2004 2544 2959 3120 513 Size: 552 939 540 415 161 4468 Fragments arranged by size: 4468 939 552 540 415 161 NdeI CA'TA_TG Cuts at: 387 387 Size: 7075 NgoMIV G'CCGG_C Cuts at: 3833 3833 Size: 7075 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7075 NotI GC'GGCC_GC Cuts at: 4522 4522 Size: 7075 NsiI A_TGCA'T Cuts at: 2322 2322 Size: 7075 NspI r_CATG'y Cuts at: 1132 1229 2324 2341 4433 5978 1132 Size: 97 1095 17 2092 1545 2229 Fragments arranged by size: 2229 2092 1545 1095 97 17 PasI CC'CwG_GG Cuts at: 1348 4238 1348 Size: 2890 4185 PciI A'CATG_T Cuts at: 2337 5974 2337 Size: 3637 3438 PflMI CCAn_nnn'nTGG Cuts at: 1108 4231 1108 Size: 3123 3952 PfoI T'CCnGG_A Cuts at: 3579 3579 Size: 7075 PmeI GTTT'AAAC Cuts at: 4379 4379 Size: 7075 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5144 5176 6657 6689 5144 Size: 32 1481 32 5530 Fragments arranged by size: 5530 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7075 PsiI TTA'TAA Cuts at: 4645 4645 Size: 7075 PspOMI G'GGCC_C Cuts at: 3280 4234 4242 4293 3280 Size: 954 8 51 6062 Fragments arranged by size: 6062 954 51 8 PstI C_TGCA'G Cuts at: 830 3614 4427 830 Size: 2784 813 3478 Fragments arranged by size: 3478 2784 813 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7075 SacI G_AGCT'C Cuts at: 4200 4200 Size: 7075 SalI G'TCGA_C Cuts at: 1936 4417 1936 Size: 2481 4594 SapI GCTCTTCn'nnn_ Cuts at: 4077 4077 Size: 7075 SbfI CC_TGCA'GG Cuts at: 4427 4427 Size: 7075 ScaI AGT'ACT Cuts at: 2921 3616 5323 7035 2921 Size: 695 1707 1712 2961 Fragments arranged by size: 2961 1712 1707 695 SfcI C'TryA_G Cuts at: 826 1045 2734 3610 3633 4423 5558 6239 6430 Size: 219 1689 876 23 790 1135 681 191 Cuts at: 6430 826 Size: 1471 Fragments arranged by size: 1689 1471 1135 876 790 681 219 191 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7075 SmaI CCC'GGG Cuts at: 1872 3559 4157 4402 1872 Size: 1687 598 245 4545 Fragments arranged by size: 4545 1687 598 245 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 3780 4138 5179 6080 6342 Size: 197 926 450 1387 358 1041 901 262 Cuts at: 6342 820 Size: 1553 Fragments arranged by size: 1553 1387 1041 926 901 450 358 262 197 SnaBI TAC'GTA Cuts at: 493 4866 493 Size: 4373 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7075 SphI G_CATG'C Cuts at: 1132 2324 4433 1132 Size: 1192 2109 3774 Fragments arranged by size: 3774 2109 1192 SrfI GCCC'GGGC Cuts at: 4157 4157 Size: 7075 SspI AAT'ATT Cuts at: 5 52 4999 5 Size: 47 4947 2081 Fragments arranged by size: 4947 2081 47 StuI AGG'CCT Cuts at: 1517 3644 1517 Size: 2127 4948 StyI C'CwwG_G Cuts at: 513 1065 2004 2544 2781 2947 2959 3120 3339 Size: 552 939 540 237 166 12 161 219 Cuts at: 3339 3997 4514 513 Size: 658 517 3074 Fragments arranged by size: 3074 939 658 552 540 517 237 219 166 161 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2557 2681 3218 3412 5102 5119 5272 Size: 1255 977 124 537 194 1690 17 153 Cuts at: 5272 5457 5902 325 Size: 185 445 1498 Fragments arranged by size: 1690 1498 1255 977 537 445 194 185 153 124 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2334 2565 2919 Size: 274 80 33 51 1443 357 231 354 Cuts at: 2919 3060 3614 3894 5321 7033 96 Size: 141 554 280 1427 1712 138 Fragments arranged by size: 1712 1443 1427 554 357 354 280 274 231 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3674 3706 7035 7067 3674 Size: 32 3329 32 3682 Fragments arranged by size: 3682 3329 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7075 XbaI T'CTAG_A Cuts at: 4411 4411 Size: 7075 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3973 4228 3973 Size: 255 6820 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7075 XmaI C'CCGG_G Cuts at: 1870 3557 4155 4400 1870 Size: 1687 598 245 4545 Fragments arranged by size: 4545 1687 598 245 XmnI GAAnn'nnTTC Cuts at: 3048 4034 4350 5204 3048 Size: 986 316 854 4919 Fragments arranged by size: 4919 986 854 316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4883 276 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SwaI