Restriction map for
pFN21AB7732
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BsaAI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsaI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1312 GTATAC GT^[AC][GT]AC
AflIII 620 ACGTGT A^C[GA][CT]GT
AgeI 1316 ACCGGT A^CCGGT
Aor51HI 1388 AGCGCT AGC^GCT
AspHI 360 GAGCTC G[AT]GC[AT]^C
AspI 3 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 496 CCTCAGC CCTCAGC(-5/-2)
BbvI 69 GCTGC GCAGC(8/12)
BglII 305 AGATCT A^GATCT
BlpI 509 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 509 GCTCAGC GC^T[ACGT]AGC
BsaBI 725 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1191 GAGACC GGTCTC(1/5)
BsaWI 1316 ACCGGT [AT]^CCGG[AT]
BsiHKAI 360 GAGCTC G[AT]GC[AT]^C
BspHI 810 TCATGA T^CATGA
BspMI 128 ACCTGC ACCTGC(4/8)
BsrFI 1316 ACCGGT [GA]^CCGG[CT]
Bst1107I 1312 GTATAC GTA^TAC
BstZ17I 1312 GTATAC GTA^TAC
Cac8I 299 GCCAGC GC[ACGT]^[ACGT]GC
CelII 509 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1316 ACCGGT [GA]^CCGG[CT]
DraI 1434 TTTAAA TTT^AAA
DraII 799 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1388 AGCGCT AGC^GCT
EcoO109I 799 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 11 CCAAGG C^C[AT][AT]GG
Fnu4HI 69 GCTGC GC^[ACGT]GC
HapII 1317 CCGG C^CGG
HpaII 1317 CCGG C^CGG
HphI 382 GGTGA GGTGA(8/7)
ItaI 69 GCTGC GC^[ACGT]GC
MaeII 620 ACGT A^CGT
MamI 725 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 444 CAATTG C^AATTG
MspI 1317 CCGG C^CGG
MunI 444 CAATTG C^AATTG
MwoI 63 GCTGGGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 125 GGAACC GG[ACGT]^[ACGT]CC
NspI 790 GCATGT [GA]CATG^[CT]
PflFI 3 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1316 ACCGGT A^CCGGT
RcaI 810 TCATGA T^CATGA
SacI 360 GAGCTC GAGCT^C
StyI 11 CCAAGG C^C[AT][AT]GG
TaqI 99 TCGA T^CGA
TseI 69 GCTGC G^C[AT]GC
Tth111I 3 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 99 TCGA T^CGA
XcmI 280 CCACATGAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanII 360 GAGCTC G[GA]GC[CT]^C
1175 GAGCCC
BmyI 360 GAGCTC G[AGT]GC[ACT]^C
1175 GAGCCC
BseRI 55 GAGGAG GAGGAG(10/8)
58 GAGGAG
Bsp1286I 360 GAGCTC G[AGT]GC[ACT]^C
1175 GAGCCC
BsrDI 748 CATTGC GCAATG(2/0)
1264 CATTGC
BstYI 242 AGATCC [GA]^GATC[CT]
305 AGATCT
CfoI 1389 GCGC GCG^C
1401 GCGC
HaeII 1388 AGCGCT [GA]GCGC^[CT]
1400 GGCGCT
HhaI 1389 GCGC GCG^C
1401 GCGC
HinP1I 1389 GCGC G^CGC
1401 GCGC
HindIII 549 AAGCTT A^AGCTT
1306 AAGCTT
MflI 242 AGATCC [GA]^GATC[CT]
305 AGATCT
SmlI 341 CTCAAG C^T[CT][GA]AG
1236 CTCAAG
StuI 337 AGGCCT AGG^CCT
1232 AGGCCT
TfiI 1248 GAATC G^A[AT]TC
1407 GAATC
XhoII 242 AGATCC [GA]^GATC[CT]
305 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AvaII BbsI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRI EcoRII FokI HaeIII
HinfI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII PleI SapI Sau3AI Sau96I
ScrFI SfaNI Tru9I Tsp45I Tsp509I
TspRI XmnI