(Circular) (Six-base) MAPSORT of: pFN21AB7732.seq Check: 8535 from: 1 to: 6151 pFN21AB7732 6151 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3318 3494 1937 Size: 1381 176 4594 Fragments arranged by size: 4594 1381 176 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2110 2458 2710 2813 3046 4214 5598 5866 Size: 478 348 252 103 233 1168 1384 268 Cuts at: 5866 1632 Size: 1917 Fragments arranged by size: 1917 1384 1168 478 348 268 252 233 103 AfeI AGC'GCT Cuts at: 3395 3395 Size: 6151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 2625 5050 6027 1203 Size: 1422 2425 977 1327 Fragments arranged by size: 2425 1422 1327 977 AgeI A'CCGG_T Cuts at: 3321 4982 3321 Size: 1661 4490 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 3894 5466 3894 Size: 1572 4579 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2233 2639 2844 3460 3686 3838 5947 1493 Size: 740 406 205 616 226 152 2109 1697 Fragments arranged by size: 2109 1697 740 616 406 226 205 152 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 3476 1870 Size: 73 1533 4545 Fragments arranged by size: 4545 1533 73 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3472 4927 618 Size: 325 217 627 1685 1455 1842 Fragments arranged by size: 1842 1685 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2369 3184 3470 1391 Size: 978 815 286 4072 Fragments arranged by size: 4072 978 815 286 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 2270 3387 928 Size: 971 145 226 1117 3692 Fragments arranged by size: 3692 1117 971 226 145 BbvCI CC'TCA_GC Cuts at: 2502 2502 Size: 6151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4342 4376 1903 Size: 34 2405 34 3678 Fragments arranged by size: 3678 2405 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4762 136 Size: 107 122 71 631 605 3090 1525 Fragments arranged by size: 3090 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2310 6146 2310 Size: 3836 2315 BlpI GC'TnA_GC Cuts at: 2515 3568 2515 Size: 1053 5098 Bme1580I G_kGCm'C Cuts at: 1790 4212 5368 1790 Size: 2422 1156 2573 Fragments arranged by size: 2573 2422 1156 BmgBI CAC'GTC Cuts at: 1318 2626 1318 Size: 1308 4843 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2967 3882 4840 Size: 416 53 83 399 24 1550 915 958 Cuts at: 4840 442 Size: 1753 Fragments arranged by size: 1753 1550 958 915 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2627 2627 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2706 3126 4811 1239 Size: 899 568 420 1685 2579 Fragments arranged by size: 2579 1685 899 568 420 Bpu10I CC'TnA_GC Cuts at: 2502 2502 Size: 6151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2331 3226 4276 5141 5439 2331 Size: 895 1050 865 298 3043 Fragments arranged by size: 3043 1050 895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3190 4814 882 Size: 617 1691 1624 2219 Fragments arranged by size: 2219 1691 1624 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 2734 6145 2734 Size: 3411 2740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3958 4340 4975 275 Size: 53 83 186 936 2425 382 635 1451 Fragments arranged by size: 2425 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3321 4584 4982 5256 5403 5814 3321 Size: 1263 398 274 147 411 3658 Fragments arranged by size: 3658 1263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 1178 Size: 30 165 30 777 30 5119 Fragments arranged by size: 5119 777 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2078 1186 Size: 889 3 5259 Fragments arranged by size: 5259 889 3 BseYI C'CCAG_C Cuts at: 2068 5354 5850 2068 Size: 3286 496 2369 Fragments arranged by size: 3286 2369 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5578 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2369 4212 4297 5368 2369 Size: 1843 85 1071 3152 Fragments arranged by size: 3152 1843 1071 85 BsmI GAATG_Cn' Cuts at: 3662 3755 3662 Size: 93 6058 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 3184 3470 4212 4297 5368 1391 Size: 399 579 815 286 742 85 1071 2174 Fragments arranged by size: 2174 1071 815 742 579 399 286 85 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 2815 4040 5770 2815 Size: 1225 1730 3196 Fragments arranged by size: 3196 1730 1225 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 3506 844 Size: 1298 1364 3489 Fragments arranged by size: 3489 1364 1298 BsrBI CCG'CTC Cuts at: 1394 1823 4038 1394 Size: 429 2215 3507 Fragments arranged by size: 3507 2215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2752 3268 4646 4820 65 Size: 1183 1504 516 1378 174 1396 Fragments arranged by size: 1504 1396 1378 1183 516 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3321 4795 4982 1700 Size: 110 1511 1474 187 2869 Fragments arranged by size: 2869 1511 1474 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6151 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstYI r'GATC_y Cuts at: 1972 2247 2310 3481 3848 4233 4250 5702 5788 Size: 275 63 1171 367 385 17 1452 86 Cuts at: 5788 5800 6146 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1171 385 367 346 275 86 63 17 12 BstZ17I GTA'TAC Cuts at: 3319 3319 Size: 6151 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5599 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 2031 2052 3667 4461 4481 2031 Size: 21 1615 794 20 3701 Fragments arranged by size: 3701 1615 794 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 3441 3455 3441 Size: 14 6137 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116 Size: 54 1425 50 275 18 1768 889 1629 Cuts at: 6116 8 Size: 43 Fragments arranged by size: 1768 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3050 3134 3218 4081 1353 Size: 1697 84 84 863 3423 Fragments arranged by size: 3423 1697 863 84 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4738 5112 5258 387 Size: 1064 138 3149 374 146 1280 Fragments arranged by size: 3149 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2367 2367 Size: 6151 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2138 2458 2706 2710 2813 3046 Size: 393 478 28 320 248 4 103 233 Cuts at: 3046 3126 4214 4811 5598 5866 1239 Size: 80 1088 597 787 268 1524 Fragments arranged by size: 1524 1088 787 597 478 393 320 268 248 233 103 80 28 4 EcoO109I rG'GnC_Cy Cuts at: 2805 2805 Size: 6151 EcoRI G'AATT_C Cuts at: 2233 2639 2844 3460 3838 2233 Size: 406 205 616 378 4546 Fragments arranged by size: 4546 616 406 378 205 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3424 3424 Size: 6151 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 3397 3409 5298 1678 Size: 1719 12 1889 2531 Fragments arranged by size: 2531 1889 1719 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2232 2264 2381 2413 2641 Size: 32 135 32 660 32 117 32 228 Cuts at: 2641 2673 2881 2913 4792 4824 4866 4898 1373 Size: 32 208 32 1879 32 42 32 2626 Fragments arranged by size: 2626 1879 660 228 208 135 117 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3741 669 Size: 988 281 1557 246 3079 Fragments arranged by size: 3079 1557 988 281 246 HindIII A'AGCT_T Cuts at: 748 2554 3311 748 Size: 1806 757 3588 Fragments arranged by size: 3588 1806 757 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 2449 3750 2449 Size: 1301 4850 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2275 5265 5449 1305 Size: 970 2990 184 2007 Fragments arranged by size: 2990 2007 970 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4329 Fragments arranged by size: 4329 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2535 2869 3121 3289 3373 4109 Size: 648 843 526 334 252 168 84 736 Cuts at: 4109 4468 4627 518 Size: 359 159 2042 Fragments arranged by size: 2042 843 736 648 526 359 334 252 168 159 84 MspA1I CmG'CkG Cuts at: 1606 3567 4244 5392 5637 1606 Size: 1961 677 1148 245 2120 Fragments arranged by size: 2120 1961 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5599 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2799 3509 5054 1132 Size: 97 1570 710 1545 2229 Fragments arranged by size: 2229 1570 1545 710 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6151 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6151 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 2784 3204 3721 2784 Size: 420 517 5214 Fragments arranged by size: 5214 517 420 PstI C_TGCA'G Cuts at: 830 3503 830 Size: 2673 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 SacI G_AGCT'C Cuts at: 2369 2369 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SapI GCTCTTCn'nnn_ Cuts at: 3050 3134 3218 3050 Size: 84 84 5983 Fragments arranged by size: 5983 84 84 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SfcI C'TryA_G Cuts at: 826 1045 3499 4634 5315 5506 826 Size: 219 2454 1135 681 191 1471 Fragments arranged by size: 2454 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2346 3241 4255 5156 5418 820 Size: 197 926 403 895 1014 901 262 1553 Fragments arranged by size: 1553 1014 926 901 895 403 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 2344 3239 1517 Size: 827 895 4429 Fragments arranged by size: 4429 895 827 StyI C'CwwG_G Cuts at: 513 1065 2016 3590 513 Size: 552 951 1574 3074 Fragments arranged by size: 3074 1574 951 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4178 4195 4348 4533 4978 325 Size: 1255 2598 17 153 185 445 1498 Fragments arranged by size: 2598 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4397 6109 96 Size: 274 80 33 51 1443 2420 1712 138 Fragments arranged by size: 2420 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6111 6143 6111 Size: 32 6119 Tth111I GACn'n_nGTC Cuts at: 1316 2011 1316 Size: 695 5456 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2292 2292 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 2696 2864 3059 4280 2696 Size: 168 195 1221 4567 Fragments arranged by size: 4567 1221 195 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI