Restriction map for
pFN21AE1073
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BglI BglII BlnI BpuAI
BsaBI BsaHI BsaI BseAI BsiWI
BsmBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Cfr10I CpoI DraII DrdI
Eam1105I Eco47III EcoNI EcoO109I EcoO65I
EcoRI EcoRV FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AflII 1774 CTTAAG C^TTAAG
Alw44I 1412 GTGCAC G^TGCAC
AlwNI 1814 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 771 CCTGAGG CC^T[ACGT]AGG
ApaLI 1412 GTGCAC G^TGCAC
Asp700I 809 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1688 CCCGAG C^[CT]CG[GA]G
AvaII 1884 GGACC G^G[AT]CC
BamHI 2116 GGATCC G^GATCC
BclI 180 TGATCA T^GATCA
BfrI 1774 CTTAAG C^TTAAG
BlpI 1789 GCTGAGC GC^T[ACGT]AGC
BpmI 1066 CTCCAG CTGGAG(16/14)
Bpu1102I 1789 GCTGAGC GC^T[ACGT]AGC
BsaAI 2404 TACGTG [CT]AC^GT[GA]
BsaWI 295 TCCGGT [AT]^CCGG[AT]
BseRI 1639 GAGGAG GAGGAG(10/8)
BsiEI 90 CGGCCG CG[GA][CT]^CG
BsmAI 751 GAGAC GTCTC(1/5)
BsoBI 1688 CCCGAG C^[CT]CG[GA]G
BspDI 1618 ATCGAT AT^CGAT
BsrDI 1752 CATTGC GCAATG(2/0)
BstAPI 1723 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1454 TTCGAA TT^CGAA
BstYI 2116 GGATCC [GA]^GATC[CT]
Bsu36I 771 CCTGAGG CC^T[ACGT]AGG
CelII 1789 GCTGAGC GC^T[ACGT]AGC
CfoI 118 GCGC GCG^C
ClaI 1618 ATCGAT AT^CGAT
DraIII 278 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 351 CCATGG C^C[GA][CT]GG
EagI 90 CGGCCG C^GGCCG
EclXI 90 CGGCCG C^GGCCG
Eco52I 90 CGGCCG C^GGCCG
Eco81I 771 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1949 ATGCAT ATGCA^T
FbaI 180 TGATCA T^GATCA
HhaI 118 GCGC GCG^C
HinP1I 118 GCGC G^CGC
MfeI 1600 CAATTG C^AATTG
MflI 2116 GGATCC [GA]^GATC[CT]
MslI 742 CATAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1600 CAATTG C^AATTG
NcoI 351 CCATGG C^CATGG
NheI 1482 GCTAGC G^CTAGC
NsiI 1949 ATGCAT ATGCA^T
NspI 676 GCATGC [GA]CATG^[CT]
NspV 1454 TTCGAA TT^CGAA
PvuII 2147 CAGCTG CAG^CTG
SalI 110 GTCGAC G^TCGAC
SapI 1768 GAAGAGC GCTCTTC(1/4)
ScaI 1663 AGTACT AGT^ACT
SfuI 1454 TTCGAA TT^CGAA
SphI 676 GCATGC GCATG^C
StuI 521 AGGCCT AGG^CCT
XhoII 2116 GGATCC [GA]^GATC[CT]
XmnI 809 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 287 AAATTC [GA]^AATT[CT]
1697 AAATTT
AlwI 2117 GATCC GGATC(4/5)
2116 GGATC
ApoI 287 AAATTC [GA]^AATT[CT]
1697 AAATTT
BalI 14 TGGCCA TGG^CCA
2417 TGGCCA
BcnI 404 CCCGG CC^[GC]GG
1635 CCGGG
BsgI 1164 CTGCAC GTGCAG(16/14)
2197 GTGCAG
DraI 1392 TTTAAA TTT^AAA
2154 TTTAAA
EarI 2162 CTCTTC CTCTTC(1/4)
1768 GAAGAG
EcoT14I 11 CCTTGG C^C[AT][AT]GG
351 CCATGG
HgaI 57 GCGTC GACGC(5/10)
1816 GCGTC
HindIII 1082 AAGCTT A^AGCTT
1246 AAGCTT
Ksp632I 2162 CTCTTC CTCTTC(1/4)
1768 GAAGAG
MaeII 323 ACGT A^CGT
2405 ACGT
MluNI 14 TGGCCA TGG^CCA
2417 TGGCCA
MscI 14 TGGCCA TGG^CCA
2417 TGGCCA
NciI 404 CCCGG CC^[GC]GG
1635 CCGGG
SacI 1063 GAGCTC GAGCT^C
2290 GAGCTC
SfcI 514 CTACAG C^T[GA][CT]AG
1334 CTACAG
StyI 11 CCTTGG C^C[AT][AT]GG
351 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccI AciI AfaI AluI AspHI
BanII BbvI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI BsmI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I