Restriction map for
pFN21AE1073
(Linear) (Six-base) MAPSORT of: KIEE1073.seq Check: 6949 from: 1 to: 2433
KIEE1073.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 0 65 111 320 2433
Size: 65 46 209 2113
Fragments arranged by size:
2113 209 65 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 550 581 769 866 2260 2335 2433
Size: 287 263 31 188 97 1394 75 98
Fragments arranged by size:
1394 287 263 188 98 97 75 31
AflII C'TTAA_G
Cuts at: 0 1774 2433
Size: 1774 659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 143 2433
Size: 143 2290
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 2433
Size: 1049 32 1352
Fragments arranged by size:
1352 1049 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1819 2433
Size: 1819 614
ApaLI G'TGCA_C
Cuts at: 0 1412 2433
Size: 1412 1021
ApoI r'AATT_y
Cuts at: 0 287 1697 2433
Size: 287 1410 736
Fragments arranged by size:
1410 736 287
AvaI C'yCGr_G
Cuts at: 0 1688 2433
Size: 1688 745
BamHI G'GATC_C
Cuts at: 0 2116 2433
Size: 2116 317
BanII G_rGCy'C
Cuts at: 0 80 1067 2294 2433
Size: 80 987 1227 139
Fragments arranged by size:
1227 987 139 80
BclI T'GATC_A
Cuts at: 0 180 2433
Size: 180 2253
BfrBI ATG'CAT
Cuts at: 0 1951 2433
Size: 1951 482
BlpI GC'TnA_GC
Cuts at: 0 1790 2433
Size: 1790 643
Bme1580I G_kGCm'C
Cuts at: 0 1416 1689 2433
Size: 1416 273 744
Fragments arranged by size:
1416 744 273
BmgBI CAC'GTC
Cuts at: 0 324 2433
Size: 324 2109
BmtI G_CTAG'C
Cuts at: 0 1486 2433
Size: 1486 947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 2433
Size: 1051 1382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1816 2122 2433
Size: 1816 306 311
Fragments arranged by size:
1816 311 306
BsaAI yAC'GTr
Cuts at: 0 2406 2433
Size: 2406 27
BsaWI w'CCGG_w
Cuts at: 0 295 2433
Size: 295 2138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 308 338 1049 1079 2433
Size: 308 30 711 30 1354
Fragments arranged by size:
1354 711 308 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1654 2433
Size: 1654 779
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1149 2218 2433
Size: 1149 1069 215
Fragments arranged by size:
1149 1069 215
BsiEI CG_ry'CG
Cuts at: 0 93 2433
Size: 93 2340
BsiHKAI G_wGCw'C
Cuts at: 0 1067 1139 1416 2294 2433
Size: 1067 72 277 878 139
Fragments arranged by size:
1067 878 277 139 72
BsmI GAATG_Cn'
Cuts at: 0 1195 1953 1969 2433
Size: 1195 758 16 464
Fragments arranged by size:
1195 758 464 16
Bsp1286I G_dGCh'C
Cuts at: 0 80 1067 1139 1416 1689 2294 2433
Size: 80 987 72 277 273 605 139
Fragments arranged by size:
987 605 277 273 139 80 72
BsrBI CCG'CTC
Cuts at: 0 95 413 1740 1800 2433
Size: 95 318 1327 60 633
Fragments arranged by size:
1327 633 318 95 60
BsrDI GCAATG_nn'
Cuts at: 0 1751 2433
Size: 1751 682
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1729 2433
Size: 1729 704
BstBI TT'CG_AA
Cuts at: 0 1455 2433
Size: 1455 978
BstYI r'GATC_y
Cuts at: 0 2116 2433
Size: 2116 317
Bsu36I CC'TnA_GG
Cuts at: 0 772 2433
Size: 772 1661
BtgI C'CryG_G
Cuts at: 0 351 2433
Size: 351 2082
BtsI GCAGTG_nn'
Cuts at: 0 1329 2433
Size: 1329 1104
ClaI AT'CG_AT
Cuts at: 0 1619 2433
Size: 1619 814
DraI TTT'AAA
Cuts at: 0 1394 2156 2433
Size: 1394 762 277
Fragments arranged by size:
1394 762 277
DraIII CAC_nnn'GTG
Cuts at: 0 283 2433
Size: 283 2150
EaeI y'GGCC_r
Cuts at: 0 14 90 2417 2433
Size: 14 76 2327 16
Fragments arranged by size:
2327 76 16 14
EagI C'GGCC_G
Cuts at: 0 90 2433
Size: 90 2343
EarI CTCTTCn'nnn_
Cuts at: 0 1763 2168 2433
Size: 1763 405 265
Fragments arranged by size:
1763 405 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1821 2433
Size: 1821 612
EcoICRI GAG'CTC
Cuts at: 0 1065 2292 2433
Size: 1065 1227 141
Fragments arranged by size:
1227 1065 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 550 581 769 866 1051 2260 2335
Size: 287 263 31 188 97 185 1209 75
Cuts at: 2335 2433
Size: 98
Fragments arranged by size:
1209 287 263 188 185 98 97 75
31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1405 1789 2433
Size: 1405 384 644
Fragments arranged by size:
1405 644 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 240 272 2433
Size: 240 32 2161
Fragments arranged by size:
2161 240 32
HincII GTy'rAC
Cuts at: 0 112 450 1873 2433
Size: 112 338 1423 560
Fragments arranged by size:
1423 560 338 112
HindIII A'AGCT_T
Cuts at: 0 1082 1246 2433
Size: 1082 164 1187
Fragments arranged by size:
1187 1082 164
MfeI C'AATT_G
Cuts at: 0 1600 2433
Size: 1600 833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1017 2433
Size: 524 493 1416
Fragments arranged by size:
1416 524 493
MscI TGG'CCA
Cuts at: 0 16 2419 2433
Size: 16 2403 14
Fragments arranged by size:
2403 16 14
MslI CAynn'nnrTG
Cuts at: 0 746 2433
Size: 746 1687
MspA1I CmG'CkG
Cuts at: 0 107 570 2149 2433
Size: 107 463 1579 284
Fragments arranged by size:
1579 463 284 107
NcoI C'CATG_G
Cuts at: 0 351 2433
Size: 351 2082
NheI G'CTAG_C
Cuts at: 0 1482 2433
Size: 1482 951
NsiI A_TGCA'T
Cuts at: 0 1953 2433
Size: 1953 480
NspI r_CATG'y
Cuts at: 0 680 2433
Size: 680 1753
PfoI T'CCnGG_A
Cuts at: 0 1634 2433
Size: 1634 799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 2433
Size: 1049 32 1352
Fragments arranged by size:
1352 1049 32
PsiI TTA'TAA
Cuts at: 0 238 2433
Size: 238 2195
PvuII CAG'CTG
Cuts at: 0 2149 2433
Size: 2149 284
SacI G_AGCT'C
Cuts at: 0 1067 2294 2433
Size: 1067 1227 139
Fragments arranged by size:
1227 1067 139
SalI G'TCGA_C
Cuts at: 0 110 2433
Size: 110 2323
SapI GCTCTTCn'nnn_
Cuts at: 0 1763 2433
Size: 1763 670
ScaI AGT'ACT
Cuts at: 0 1665 2433
Size: 1665 768
SfcI C'TryA_G
Cuts at: 0 514 1334 2433
Size: 514 820 1099
Fragments arranged by size:
1099 820 514
SmlI C'TyrA_G
Cuts at: 0 1774 1795 2101 2433
Size: 1774 21 306 332
Fragments arranged by size:
1774 332 306 21
SphI G_CATG'C
Cuts at: 0 680 2433
Size: 680 1753
StuI AGG'CCT
Cuts at: 0 523 2433
Size: 523 1910
StyI C'CwwG_G
Cuts at: 0 11 351 2433
Size: 11 340 2082
Fragments arranged by size:
2082 340 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 344 601 2433
Size: 344 257 1832
Fragments arranged by size:
1832 344 257
TatI w'GTAC_w
Cuts at: 0 479 1663 2226 2433
Size: 479 1184 563 207
Fragments arranged by size:
1184 563 479 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 2433
Size: 308 32 2093
Fragments arranged by size:
2093 308 32
XmnI GAAnn'nnTTC
Cuts at: 0 813 2433
Size: 813 1620
Enzymes that do cut:
AccI AcuI AflII AlfI AloI AlwNI ApaLI ApoI
AvaI BamHI BanII BclI BfrBI BlpI Bme1580I BmgBI
BmtI BpmI BpuEI BsaAI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BstAPI BstBI
BstYI Bsu36I BtgI BtsI ClaI DraI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI FalI Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NheI
NsiI NspI PfoI PpiI PsiI PvuII SacI SalI
SapI ScaI SfcI SmlI SphI StuI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbsI BbvCI BcgI BciVI BglI BglII BmrI
BplI Bpu10I BsaI BsaBI BsaHI BseYI BsiWI BsmBI
BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstEII
BstXI BstZ17I BtgZI CspCI DrdI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NotI NruI PacI
PasI PciI PflMI PmeI PmlI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI