(Circular) (Six-base) MAPSORT of: pFN21AE1072.seq Check: 4069 from: 1 to: 7018 pFN21AE1072 7018 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4828 278 Size: 53 83 186 936 3292 2468 Fragments arranged by size: 3292 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4361 1937 Size: 2424 4594 Acc65I G'GTAC_C Cuts at: 4339 4339 Size: 7018 AclI AA'CG_TT Cuts at: 1563 5145 5518 1563 Size: 3582 373 3063 Fragments arranged by size: 3582 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4197 5081 6465 6733 1632 Size: 2565 884 1384 268 1917 Fragments arranged by size: 2565 1917 1384 884 268 AfeI AGC'GCT Cuts at: 3192 4051 3192 Size: 859 6159 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6821 AflIII A'CryG_T Cuts at: 1203 2850 3443 5917 6894 1203 Size: 1647 593 2474 977 1327 Fragments arranged by size: 2474 1647 1327 977 593 AgeI A'CCGG_T Cuts at: 5849 5849 Size: 7018 AhdI GACnn_n'nnGTC Cuts at: 2261 3044 5747 2261 Size: 783 2703 3532 Fragments arranged by size: 3532 2703 783 AleI CACnn'nnGTG Cuts at: 1166 2168 3836 1166 Size: 1002 1668 4348 Fragments arranged by size: 4348 1668 1002 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2800 1184 Size: 1616 5402 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2723 2755 3449 3481 2723 Size: 32 694 32 6260 Fragments arranged by size: 6260 694 32 32 AlwNI CAG_nnn'CTG Cuts at: 2258 2357 2508 4761 6333 2258 Size: 99 151 2253 1572 2943 Fragments arranged by size: 2943 2253 1572 151 99 ApaI G_GGCC'C Cuts at: 4173 4173 Size: 7018 ApaLI G'TGCA_C Cuts at: 5075 6231 5075 Size: 1156 5862 ApoI r'AATT_y Cuts at: 1493 2390 2629 3008 4327 4553 4705 6814 1493 Size: 897 239 379 1319 226 152 2109 1697 Fragments arranged by size: 2109 1697 1319 897 379 239 226 152 AseI AT'TA_AT Cuts at: 160 1032 5572 160 Size: 872 4540 1606 Fragments arranged by size: 4540 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7018 AvaI C'yCGr_G Cuts at: 1870 1943 4053 4343 1870 Size: 73 2110 290 4545 Fragments arranged by size: 4545 2110 290 73 BamHI G'GATC_C Cuts at: 2079 3969 4348 4715 2079 Size: 1890 379 367 4382 Fragments arranged by size: 4382 1890 379 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2935 3539 4339 5794 618 Size: 325 217 627 1148 604 800 1455 1842 Fragments arranged by size: 1842 1455 1148 800 627 604 325 217 BanII G_rGCy'C Cuts at: 1391 3111 4173 4337 1391 Size: 1720 1062 164 4072 Fragments arranged by size: 4072 1720 1062 164 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4185 928 Size: 971 2286 3761 Fragments arranged by size: 3761 2286 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5209 5243 1903 Size: 34 3272 34 3678 Fragments arranged by size: 3678 3272 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4910 6120 4910 Size: 1210 5808 BclI T'GATC_A Cuts at: 1548 3825 1548 Size: 2277 4741 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3657 5629 136 Size: 107 122 71 631 605 1985 1972 1525 Fragments arranged by size: 1985 1972 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7013 7013 Size: 7018 BlpI GC'TnA_GC Cuts at: 4435 4435 Size: 7018 Bme1580I G_kGCm'C Cuts at: 1790 2210 3693 4173 5079 6235 1790 Size: 420 1483 480 906 1156 2573 Fragments arranged by size: 2573 1483 1156 906 480 420 BmgBI CAC'GTC Cuts at: 1318 2853 3133 4096 1318 Size: 1535 280 963 4240 Fragments arranged by size: 4240 1535 963 280 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2130 2734 4171 Size: 416 53 83 399 24 713 604 1437 Cuts at: 4171 4749 5707 442 Size: 578 958 1753 Fragments arranged by size: 1753 1437 958 713 604 578 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2153 1056 Size: 1097 5921 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4144 4144 Size: 7018 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2059 2447 2847 4296 5678 1239 Size: 820 388 400 1449 1382 2579 Fragments arranged by size: 2579 1449 1382 820 400 388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3235 5143 6008 6306 3235 Size: 1908 865 298 3947 Fragments arranged by size: 3947 1908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2230 3213 3274 5681 882 Size: 617 731 983 61 2407 2219 Fragments arranged by size: 2407 2219 983 731 617 61 BsaAI yAC'GTr Cuts at: 493 1204 4080 4809 493 Size: 711 2876 729 2702 Fragments arranged by size: 2876 2702 729 711 BsaBI GATnn'nnATC Cuts at: 3239 7012 3239 Size: 3773 3245 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2310 4825 5207 5842 Size: 53 83 186 936 777 2515 382 635 Cuts at: 5842 275 Size: 1451 Fragments arranged by size: 2515 1451 936 777 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3972 5451 5849 6123 6270 6681 3972 Size: 1479 398 274 147 411 4309 Fragments arranged by size: 4309 1479 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2322 2352 2723 2753 3122 Size: 30 165 30 919 30 371 30 369 Cuts at: 3122 3152 1178 Size: 30 5044 Fragments arranged by size: 5044 919 371 369 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3185 3356 4133 1186 Size: 1999 171 777 4071 Fragments arranged by size: 4071 1999 777 171 BseYI C'CCAG_C Cuts at: 3055 3430 3646 6221 6717 3055 Size: 375 216 2575 496 3356 Fragments arranged by size: 3356 2575 496 375 216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 4052 1163 Size: 573 327 1989 4129 Fragments arranged by size: 4129 1989 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2451 4228 4468 5229 6257 1540 Size: 275 186 450 1777 240 761 1028 2301 Fragments arranged by size: 2301 1777 1028 761 450 275 240 186 BsiHKAI G_wGCw'C Cuts at: 2464 3348 3714 5079 5164 6235 2464 Size: 884 366 1365 85 1071 3247 Fragments arranged by size: 3247 1365 1071 884 366 85 BsmI GAATG_Cn' Cuts at: 3366 4529 4622 3366 Size: 1163 93 5762 Fragments arranged by size: 5762 1163 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2464 3111 3348 3693 3714 4173 Size: 399 420 254 647 237 345 21 459 Cuts at: 4173 4337 5079 5164 6235 1391 Size: 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 647 459 420 399 345 254 237 164 85 21 BspEI T'CCGG_A Cuts at: 6681 6681 Size: 7018 BspHI T'CATG_A Cuts at: 2688 4907 6637 2688 Size: 2219 1730 3069 Fragments arranged by size: 3069 2219 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4373 844 Size: 3529 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4905 1394 Size: 429 3082 3507 Fragments arranged by size: 3507 3082 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2073 2280 5513 5687 65 Size: 1183 825 207 3233 174 1396 Fragments arranged by size: 3233 1396 1183 825 207 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3669 5662 5849 1700 Size: 110 1859 1993 187 2869 Fragments arranged by size: 2869 1993 1859 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7018 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7018 BssSI C'ACGA_G Cuts at: 5078 6090 5078 Size: 1012 6006 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7018 BstBI TT'CG_AA Cuts at: 5822 5822 Size: 7018 BstEII G'GTnAC_C Cuts at: 1184 3697 1184 Size: 2513 4505 BstXI CCAn_nnnn'nTGG Cuts at: 2098 2248 3802 2098 Size: 150 1554 5314 Fragments arranged by size: 5314 1554 150 BstYI r'GATC_y Cuts at: 1972 2079 2354 3969 4348 4715 5100 5117 6569 Size: 107 275 1615 379 367 385 17 1452 Cuts at: 6569 6655 6667 7013 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1615 1452 385 379 367 346 275 107 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2130 2749 3093 3543 513 Size: 552 1065 619 344 450 3988 Fragments arranged by size: 3988 1065 619 552 450 344 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2800 487 Size: 683 56 1574 4705 Fragments arranged by size: 4705 1574 683 56 BtsI GCAGTG_nn' Cuts at: 2051 2442 2871 4534 5328 5348 2051 Size: 391 429 1663 794 20 3721 Fragments arranged by size: 3721 1663 794 429 391 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7018 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3427 3462 529 Size: 35 2863 35 4085 Fragments arranged by size: 4085 2863 35 35 DraI TTT'AAA Cuts at: 4322 4322 Size: 7018 DraIII CAC_nnn'GTG Cuts at: 3697 3697 Size: 7018 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6025 809 Size: 507 4709 1802 Fragments arranged by size: 4709 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 2448 3018 Size: 54 1425 50 275 18 408 210 570 Cuts at: 3018 4225 4465 5354 6983 8 Size: 1207 240 889 1629 43 Fragments arranged by size: 1629 1425 1207 889 570 408 275 240 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2448 4225 4465 1537 Size: 275 636 1777 240 4090 Fragments arranged by size: 4090 1777 636 275 240 EarI CTCTTCn'nnn_ Cuts at: 1353 2708 3071 3725 4053 4241 4948 1353 Size: 1355 363 654 328 188 707 3423 Fragments arranged by size: 3423 1355 707 654 363 328 188 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2531 2744 5605 5979 6125 387 Size: 1064 138 942 213 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 942 374 213 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2447 2847 4197 4296 5081 5678 Size: 393 427 388 400 1350 99 785 597 Cuts at: 5678 6465 6733 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1350 787 785 597 427 400 393 388 268 99 EcoO109I rG'GnC_Cy Cuts at: 2255 3060 4063 4279 2255 Size: 805 1003 216 4994 Fragments arranged by size: 4994 1003 805 216 EcoRI G'AATT_C Cuts at: 2629 4327 4705 2629 Size: 1698 378 4942 Fragments arranged by size: 4942 1698 378 FspI TGC'GCA Cuts at: 5524 5524 Size: 7018 HaeII r_GCGC'y Cuts at: 1678 3194 4053 6165 1678 Size: 1516 859 2112 2531 Fragments arranged by size: 2531 2112 1516 859 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2247 2279 2322 Size: 32 135 32 593 32 50 32 43 Cuts at: 2322 2354 2911 2943 2945 2977 3030 3062 3221 Size: 32 557 32 2 32 53 32 159 Cuts at: 3221 3253 3604 3636 3758 3790 4008 4040 4094 Size: 32 351 32 122 32 218 32 54 Cuts at: 4094 4126 5659 5691 5733 5765 1373 Size: 32 1533 32 42 32 2626 Fragments arranged by size: 2626 1533 593 557 351 218 159 135 122 54 53 50 43 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 669 1657 1938 3510 3523 4362 4608 669 Size: 988 281 1572 13 839 246 3079 Fragments arranged by size: 3079 1572 988 839 281 246 13 HindIII A'AGCT_T Cuts at: 748 3390 748 Size: 2642 4376 HpaI GTT'AAC Cuts at: 4608 4608 Size: 7018 KpnI G_GTAC'C Cuts at: 4343 4343 Size: 7018 MfeI C'AATT_G Cuts at: 4617 4617 Size: 7018 MluI A'CGCG_T Cuts at: 6894 6894 Size: 7018 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6132 6316 1305 Size: 4827 184 2007 Fragments arranged by size: 4827 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2240 3020 10 Size: 54 1425 343 408 780 4008 Fragments arranged by size: 4008 1425 780 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2213 2219 2975 3707 3728 3836 Size: 648 1002 45 6 756 732 21 108 Cuts at: 3836 3926 4976 5335 5494 518 Size: 90 1050 359 159 2042 Fragments arranged by size: 2042 1050 1002 756 732 648 359 159 108 90 45 21 6 MspA1I CmG'CkG Cuts at: 1606 2199 2508 4434 5111 6259 6504 1606 Size: 593 309 1926 677 1148 245 2120 Fragments arranged by size: 2120 1926 1148 677 593 309 245 NcoI C'CATG_G Cuts at: 513 1065 3093 513 Size: 552 2028 4438 Fragments arranged by size: 4438 2028 552 NdeI CA'TA_TG Cuts at: 387 2902 387 Size: 2515 4503 NheI G'CTAG_C Cuts at: 1052 2149 1052 Size: 1097 5921 NotI GC'GGCC_GC Cuts at: 4465 4465 Size: 7018 NspI r_CATG'y Cuts at: 1132 1229 2218 2476 2787 3447 4376 5921 1132 Size: 97 989 258 311 660 929 1545 2229 Fragments arranged by size: 2229 1545 989 929 660 311 258 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7018 PciI A'CATG_T Cuts at: 3443 5917 3443 Size: 2474 4544 PflMI CCAn_nnn'nTGG Cuts at: 1108 3966 1108 Size: 2858 4160 PfoI T'CCnGG_A Cuts at: 2075 2424 2075 Size: 349 6669 PmeI GTTT'AAAC Cuts at: 4322 4322 Size: 7018 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2723 2755 5087 5119 6600 6632 2723 Size: 32 2332 32 1481 32 3109 Fragments arranged by size: 3109 2332 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2255 3060 2255 Size: 805 6213 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7018 PsiI TTA'TAA Cuts at: 4588 4588 Size: 7018 PspOMI G'GGCC_C Cuts at: 4169 4169 Size: 7018 PstI C_TGCA'G Cuts at: 830 3855 4370 830 Size: 3025 515 3478 Fragments arranged by size: 3478 3025 515 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7018 PvuII CAG'CTG Cuts at: 2199 2199 Size: 7018 SalI G'TCGA_C Cuts at: 1936 4360 1936 Size: 2424 4594 SanDI GG'GwC_CC Cuts at: 3060 3060 Size: 7018 SapI GCTCTTCn'nnn_ Cuts at: 3725 4241 3725 Size: 516 6502 SbfI CC_TGCA'GG Cuts at: 4370 4370 Size: 7018 ScaI AGT'ACT Cuts at: 5266 6978 5266 Size: 1712 5306 SexAI A'CCwGG_T Cuts at: 2257 2257 Size: 7018 SfcI C'TryA_G Cuts at: 826 1045 2368 3851 4366 5501 6182 6373 826 Size: 219 1323 1483 515 1135 681 191 1471 Fragments arranged by size: 1483 1471 1323 1135 681 515 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7018 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7018 SmaI CCC'GGG Cuts at: 1872 4345 1872 Size: 2473 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3214 4053 5122 6023 6285 820 Size: 197 926 1271 839 1069 901 262 1553 Fragments arranged by size: 1553 1271 1069 926 901 839 262 197 SnaBI TAC'GTA Cuts at: 493 4809 493 Size: 4316 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7018 SphI G_CATG'C Cuts at: 1132 4376 1132 Size: 3244 3774 SspI AAT'ATT Cuts at: 5 52 3586 4942 5 Size: 47 3534 1356 2081 Fragments arranged by size: 3534 2081 1356 47 StuI AGG'CCT Cuts at: 1517 2700 1517 Size: 1183 5835 StyI C'CwwG_G Cuts at: 513 1065 2241 3093 3264 3468 4457 513 Size: 552 1176 852 171 204 989 3074 Fragments arranged by size: 3074 1176 989 852 552 204 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2668 5045 5062 5215 5400 5845 325 Size: 1255 1088 2377 17 153 185 445 1498 Fragments arranged by size: 2377 1498 1255 1088 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5264 6976 96 Size: 274 80 33 51 1443 3287 1712 138 Fragments arranged by size: 3287 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3122 3154 6978 7010 3122 Size: 32 3824 32 3130 Fragments arranged by size: 3824 3130 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7018 XbaI T'CTAG_A Cuts at: 4354 4354 Size: 7018 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3013 3013 Size: 7018 XhoI C'TCGA_G Cuts at: 1943 4053 1943 Size: 2110 4908 XmaI C'CCGG_G Cuts at: 1870 4343 1870 Size: 2473 4545 XmnI GAAnn'nnTTC Cuts at: 5147 5147 Size: 7018 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4826 276 Size: 53 83 186 936 3292 2468 Fragments arranged by size: 3292 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI