Restriction map for
pFN21AE1075
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Aor51HI
ApaI AscI Asp718I AspI AviII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
Cfr10I CpoI DraII DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MamI
MflI MluI MluNI MroI MscI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 54 enzymes) #
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AciI 1453 GCGG CCGC(-3/-1)
AflIII 850 ACACGT A^C[GA][CT]GT
AhdI 7 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1005 GTGCAC G^TGCAC
AlwI 511 GGATC GGATC(4/5)
AlwNI 1019 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 49 CCTGAGG CC^T[ACGT]AGG
ApaLI 1005 GTGCAC G^TGCAC
Asp700I 1222 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 7 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1005 GTGCAC G[AT]GC[AT]^C
AvaI 9 CCCGGG C^[CT]CG[GA]G
AvrII 1804 CCTAGG C^CTAGG
BbvI 1471 GCTGC GCAGC(8/12)
BlnI 1804 CCTAGG C^CTAGG
BmyI 1005 GTGCAC G[AGT]GC[ACT]^C
BsgI 1025 CTGCAC GTGCAG(16/14)
BsiHKAI 1005 GTGCAC G[AT]GC[AT]^C
BsmI 930 GAATGC GAATGC(1/-1)
BsoBI 9 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1005 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 2152 TGTACA T^GTACA
BsrDI 2097 CATTGC GCAATG(2/0)
BsrGI 2152 TGTACA T^GTACA
BstNI 1185 CCTGG CC^[AT]GG
BstXI 400 CCAGATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 49 CCTGAGG CC^T[ACGT]AGG
CfoI 170 GCGC GCG^C
Eam1105I 7 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 49 CCTGAGG CC^T[ACGT]AGG
EcoRII 1185 CCTGG ^CC[AT]GG
HaeII 169 AGCGCC [GA]GCGC^[CT]
HapII 10 CCGG C^CGG
HhaI 170 GCGC GCG^C
HinP1I 170 GCGC G^CGC
HpaII 10 CCGG C^CGG
MspA1I 41 CAGCTG C[AC]G^C[GT]G
MspI 10 CCGG C^CGG
MvaI 1185 CCTGG CC^[AT]GG
MwoI 1471 GCTGCTACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 61 GAGTC GAGTC(4/5)
PstI 291 CTGCAG CTGCA^G
PvuII 41 CAGCTG CAG^CTG
SapI 1798 GCTCTTC GCTCTTC(1/4)
ScaI 1611 AGTACT AGT^ACT
SmaI 9 CCCGGG CCC^GGG
SphI 1488 GCATGC GCATG^C
SspBI 2152 TGTACA T^GTACA
TseI 1471 GCTGC G^C[AT]GC
Tsp45I 1275 GTCAC ^GT[GC]AC
XcmI 1679 CCATAAGAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
XmnI 1222 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AseI 1309 ATTAAT AT^TAAT
1558 ATTAAT
AsnI 1309 ATTAAT AT^TAAT
1558 ATTAAT
AvaII 109 GGACC G^G[AT]CC
1441 GGACC
BbsI 82 GAAGAC GAAGAC(2/6)
2323 GTCTTC
BclI 1667 TGATCA T^GATCA
1742 TGATCA
BcnI 9 CCCGG CC^[GC]GG
10 CCGGG
BpuAI 82 GAAGAC GAAGAC(2/6)
2323 GTCTTC
BsaI 5 GAGACC GGTCTC(1/5)
117 GAGACC
BsmAI 5 GAGAC GTCTC(1/5)
117 GAGAC
BsmFI 1440 GGGAC GGGAC(10/14)
34 GTCCC
BspDI 136 ATCGAT AT^CGAT
1262 ATCGAT
ClaI 136 ATCGAT AT^CGAT
1262 ATCGAT
DsaI 645 CCATGG C^C[GA][CT]GG
1939 CCATGG
EcoT22I 1490 ATGCAT ATGCA^T
1510 ATGCAT
FbaI 1667 TGATCA T^GATCA
1742 TGATCA
Fnu4HI 1453 GCGGC GC^[ACGT]GC
1471 GCTGC
HaeIII 46 GGCC GG^CC
1916 GGCC
HindIII 599 AAGCTT A^AGCTT
1378 AAGCTT
ItaI 1453 GCGGC GC^[ACGT]GC
1471 GCTGC
MaeII 852 ACGT A^CGT
1674 ACGT
MfeI 1270 CAATTG C^AATTG
2338 CAATTG
MslI 529 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
984 CACCCTAGTG
MunI 1270 CAATTG C^AATTG
2338 CAATTG
NciI 9 CCCGG CC^[GC]GG
10 CCGGG
NcoI 645 CCATGG C^CATGG
1939 CCATGG
NsiI 1490 ATGCAT ATGCA^T
1510 ATGCAT
PflMI 542 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2277 CCACAGGTTGG
PshBI 1309 ATTAAT AT^TAAT
1558 ATTAAT
Van91I 542 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2277 CCACAGGTTGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BpmI BsaJI BsiYI BslI BsrI
BssKI BstF5I Cac8I Cfr13I DdeI
DpnII DraI EarI EcoT14I FokI
HincII HindII HinfI HphI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I