(Circular) (Six-base) MAPSORT of: pFN21AE1075.seq Check: 7951 from: 1 to: 7066 pFN21AE1075 7066 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4876 278 Size: 53 83 186 936 3340 2468 Fragments arranged by size: 3340 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4409 1937 Size: 2472 4594 Acc65I G'GTAC_C Cuts at: 4387 4387 Size: 7066 AclI AA'CG_TT Cuts at: 1563 5193 5566 1563 Size: 3630 373 3063 Fragments arranged by size: 3630 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4140 5129 6513 6781 1632 Size: 2508 989 1384 268 1917 Fragments arranged by size: 2508 1917 1384 989 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6869 AflIII A'CryG_T Cuts at: 1203 2855 5965 6942 1203 Size: 1652 3110 977 1327 Fragments arranged by size: 3110 1652 1327 977 AgeI A'CCGG_T Cuts at: 5897 5897 Size: 7066 AhdI GACnn_n'nnGTC Cuts at: 2017 5795 2017 Size: 3778 3288 AleI CACnn'nnGTG Cuts at: 1166 2993 1166 Size: 1827 5239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7066 AlwNI CAG_nnn'CTG Cuts at: 3029 4809 6381 3029 Size: 1780 1572 3714 Fragments arranged by size: 3714 1780 1572 ApaLI G'TGCA_C Cuts at: 3010 5123 6279 3010 Size: 2113 1156 3797 Fragments arranged by size: 3797 2113 1156 ApoI r'AATT_y Cuts at: 1493 2658 3151 3229 3342 3608 3700 3794 3984 Size: 1165 493 78 113 266 92 94 190 Cuts at: 3984 4022 4213 4375 4601 4753 6862 1493 Size: 38 191 162 226 152 2109 1697 Fragments arranged by size: 2109 1697 1165 493 266 226 191 190 162 152 113 94 92 78 38 AseI AT'TA_AT Cuts at: 160 1032 3315 3564 5620 160 Size: 872 2283 249 2056 1606 Fragments arranged by size: 2283 2056 1606 872 249 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7066 AvaI C'yCGr_G Cuts at: 1870 1943 2014 4391 1870 Size: 73 71 2377 4545 Fragments arranged by size: 4545 2377 73 71 AvrII C'CTAG_G Cuts at: 3809 3809 Size: 7066 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4290 4323 4290 Size: 33 7033 BamHI G'GATC_C Cuts at: 4396 4763 4396 Size: 367 6699 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4387 5842 618 Size: 325 217 627 2600 1455 1842 Fragments arranged by size: 2600 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4385 1391 Size: 2994 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2094 4321 928 Size: 971 195 2227 3673 Fragments arranged by size: 3673 2227 971 195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5257 5291 1903 Size: 34 3320 34 3678 Fragments arranged by size: 3678 3320 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4958 6168 4958 Size: 1210 5856 BclI T'GATC_A Cuts at: 1548 3672 3747 1548 Size: 2124 75 4867 Fragments arranged by size: 4867 2124 75 BfrBI ATG'CAT Cuts at: 3497 3517 3497 Size: 20 7046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5677 136 Size: 107 122 71 631 605 4005 1525 Fragments arranged by size: 4005 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7061 7061 Size: 7066 BlpI GC'TnA_GC Cuts at: 4483 4483 Size: 7066 Bme1580I G_kGCm'C Cuts at: 1790 3014 5127 6283 1790 Size: 1224 2113 1156 2573 Fragments arranged by size: 2573 2113 1224 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4229 4797 5755 Size: 416 53 83 399 24 2812 568 958 Cuts at: 5755 442 Size: 1753 Fragments arranged by size: 2812 1753 958 568 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7066 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2028 2990 3225 5726 1239 Size: 789 962 235 2501 2579 Fragments arranged by size: 2579 2501 962 789 235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5191 6056 6354 5191 Size: 865 298 5903 Fragments arranged by size: 5903 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2004 2116 5729 882 Size: 617 505 112 3613 2219 Fragments arranged by size: 3613 2219 617 505 112 BsaAI yAC'GTr Cuts at: 493 1204 4857 493 Size: 711 3653 2702 Fragments arranged by size: 3653 2702 711 BsaBI GATnn'nnATC Cuts at: 7060 7060 Size: 7066 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4873 5255 5890 275 Size: 53 83 186 936 3340 382 635 1451 Fragments arranged by size: 3340 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5499 5897 6171 6318 6729 5499 Size: 398 274 147 411 5836 Fragments arranged by size: 5836 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6841 Fragments arranged by size: 6841 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7066 BseYI C'CCAG_C Cuts at: 6269 6765 6269 Size: 496 6570 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3015 1163 Size: 573 1279 5214 Fragments arranged by size: 5214 1279 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4516 5277 6305 1540 Size: 275 186 2515 761 1028 2301 Fragments arranged by size: 2515 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3014 5127 5212 6283 3014 Size: 2113 85 1071 3797 Fragments arranged by size: 3797 2113 1071 85 BsmI GAATG_Cn' Cuts at: 2941 4577 4670 2941 Size: 1636 93 5337 Fragments arranged by size: 5337 1636 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3014 4385 5127 5212 6283 1391 Size: 399 1224 1371 742 85 1071 2174 Fragments arranged by size: 2174 1371 1224 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6729 6729 Size: 7066 BspHI T'CATG_A Cuts at: 4955 6685 4955 Size: 1730 5336 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4421 844 Size: 3577 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4953 1394 Size: 429 3130 3507 Fragments arranged by size: 3507 3130 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4101 5561 5735 65 Size: 1183 2853 1460 174 1396 Fragments arranged by size: 2853 1460 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5710 5897 1700 Size: 110 3900 187 2869 Fragments arranged by size: 3900 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4157 96 Size: 4061 3005 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7066 BssSI C'ACGA_G Cuts at: 5126 6138 5126 Size: 1012 6054 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7066 BstBI TT'CG_AA Cuts at: 5870 5870 Size: 7066 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7066 BstXI CCAn_nnnn'nTGG Cuts at: 2412 2412 Size: 7066 BstYI r'GATC_y Cuts at: 1972 4396 4763 5148 5165 6617 6703 6715 7061 Size: 2424 367 385 17 1452 86 12 346 Cuts at: 7061 1972 Size: 1977 Fragments arranged by size: 2424 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2055 2055 Size: 7066 BtgI C'CryG_G Cuts at: 513 1065 2004 2650 3944 513 Size: 552 939 646 1294 3635 Fragments arranged by size: 3635 1294 939 646 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6327 Fragments arranged by size: 6327 683 56 BtsI GCAGTG_nn' Cuts at: 2293 4582 5376 5396 2293 Size: 2289 794 20 3963 Fragments arranged by size: 3963 2289 794 20 ClaI AT'CG_AT Cuts at: 1554 2142 3268 1554 Size: 588 1126 5352 Fragments arranged by size: 5352 1126 588 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7031 DraI TTT'AAA Cuts at: 2241 2850 3057 3114 3261 3324 3705 3766 3990 Size: 609 207 57 147 63 381 61 224 Cuts at: 3990 4370 2241 Size: 380 4937 Fragments arranged by size: 4937 609 381 380 224 207 147 63 61 57 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6073 809 Size: 507 4757 1802 Fragments arranged by size: 4757 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4513 5402 7031 Size: 54 1425 50 275 18 2683 889 1629 Cuts at: 7031 8 Size: 43 Fragments arranged by size: 2683 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4513 1537 Size: 275 2701 4090 Fragments arranged by size: 4090 2701 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2925 3542 3810 4996 1353 Size: 1572 617 268 1186 3423 Fragments arranged by size: 3423 1572 1186 617 268 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5653 6027 6173 387 Size: 1064 138 4064 374 146 1280 Fragments arranged by size: 4064 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2990 3225 4140 5129 5726 6513 Size: 393 396 962 235 915 989 597 787 Cuts at: 6513 6781 1239 Size: 268 1524 Fragments arranged by size: 1524 989 962 915 787 597 396 393 268 235 EcoRI G'AATT_C Cuts at: 4375 4753 4375 Size: 378 6688 FspI TGC'GCA Cuts at: 5572 5572 Size: 7066 HaeII r_GCGC'y Cuts at: 1678 2178 6213 1678 Size: 500 4035 2531 Fragments arranged by size: 4035 2531 500 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2275 2307 2951 Size: 32 135 32 431 32 240 32 644 Cuts at: 2951 2983 3281 3313 4178 4210 5707 5739 5781 Size: 32 298 32 865 32 1497 32 42 Cuts at: 5781 5813 1373 Size: 32 2626 Fragments arranged by size: 2626 1497 865 644 431 298 240 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2070 3072 4320 4410 4656 669 Size: 988 281 132 1002 1248 90 246 3079 Fragments arranged by size: 3079 1248 1002 988 281 246 132 90 HindIII A'AGCT_T Cuts at: 748 2604 3383 748 Size: 1856 779 4431 Fragments arranged by size: 4431 1856 779 HpaI GTT'AAC Cuts at: 4656 4656 Size: 7066 KpnI G_GTAC'C Cuts at: 4391 4391 Size: 7066 MfeI C'AATT_G Cuts at: 3275 4343 4665 3275 Size: 1068 322 5676 Fragments arranged by size: 5676 1068 322 MluI A'CGCG_T Cuts at: 6942 6942 Size: 7066 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 2393 6180 6364 1305 Size: 696 392 3787 184 2007 Fragments arranged by size: 3787 2007 696 392 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5244 Fragments arranged by size: 5244 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2538 2993 5024 5383 5542 518 Size: 648 1372 455 2031 359 159 2042 Fragments arranged by size: 2042 2031 1372 648 455 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 4482 5159 6307 6552 1606 Size: 442 2434 677 1148 245 2120 Fragments arranged by size: 2434 2120 1148 677 442 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2650 3944 513 Size: 552 939 646 1294 3635 Fragments arranged by size: 3635 1294 939 646 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7066 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7066 NotI GC'GGCC_GC Cuts at: 4513 4513 Size: 7066 NsiI A_TGCA'T Cuts at: 3499 3519 3499 Size: 20 7046 NspI r_CATG'y Cuts at: 1132 1229 2537 3465 3497 4424 5969 1132 Size: 97 1308 928 32 927 1545 2229 Fragments arranged by size: 2229 1545 1308 928 927 97 32 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7066 PciI A'CATG_T Cuts at: 5965 5965 Size: 7066 PflMI CCAn_nnn'nTGG Cuts at: 1108 2553 4288 1108 Size: 1445 1735 3886 Fragments arranged by size: 3886 1735 1445 PmeI GTTT'AAAC Cuts at: 4370 4370 Size: 7066 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5135 5167 6648 6680 5135 Size: 32 1481 32 5521 Fragments arranged by size: 5521 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7066 PsiI TTA'TAA Cuts at: 4636 4636 Size: 7066 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2479 2511 2479 Size: 32 7034 PstI C_TGCA'G Cuts at: 830 2300 4418 830 Size: 1470 2118 3478 Fragments arranged by size: 3478 2118 1470 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7066 PvuII CAG'CTG Cuts at: 2048 2048 Size: 7066 SalI G'TCGA_C Cuts at: 1936 4408 1936 Size: 2472 4594 SapI GCTCTTCn'nnn_ Cuts at: 3810 3810 Size: 7066 SbfI CC_TGCA'GG Cuts at: 4418 4418 Size: 7066 ScaI AGT'ACT Cuts at: 3618 5314 7026 3618 Size: 1696 1712 3658 Fragments arranged by size: 3658 1712 1696 SfcI C'TryA_G Cuts at: 826 1045 2296 2970 3075 3396 4293 4414 5549 Size: 219 1251 674 105 321 897 121 1135 Cuts at: 5549 6230 6421 826 Size: 681 191 1471 Fragments arranged by size: 1471 1251 1135 897 681 674 321 219 191 121 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7066 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7066 SmaI CCC'GGG Cuts at: 1872 2016 4393 1872 Size: 144 2377 4545 Fragments arranged by size: 4545 2377 144 SmlI C'TyrA_G Cuts at: 820 1017 1943 5170 6071 6333 820 Size: 197 926 3227 901 262 1553 Fragments arranged by size: 3227 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4857 493 Size: 4364 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7066 SphI G_CATG'C Cuts at: 1132 3497 4424 1132 Size: 2365 927 3774 Fragments arranged by size: 3774 2365 927 SspI AAT'ATT Cuts at: 5 52 2731 2923 3140 3330 3652 4990 5 Size: 47 2679 192 217 190 322 1338 2081 Fragments arranged by size: 2679 2081 1338 322 217 192 190 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7066 StyI C'CwwG_G Cuts at: 513 1065 2004 2547 2650 3809 3944 4505 513 Size: 552 939 543 103 1159 135 561 3074 Fragments arranged by size: 3074 1159 939 561 552 543 135 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5093 5110 5263 5448 5893 325 Size: 1255 3513 17 153 185 445 1498 Fragments arranged by size: 3513 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3616 4157 5312 Size: 274 80 33 51 1443 1639 541 1155 Cuts at: 5312 7024 96 Size: 1712 138 Fragments arranged by size: 1712 1639 1443 1155 541 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3940 3972 7026 7058 3940 Size: 32 3054 32 3948 Fragments arranged by size: 3948 3054 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7066 XbaI T'CTAG_A Cuts at: 4402 4402 Size: 7066 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3691 3691 Size: 7066 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7066 XmaI C'CCGG_G Cuts at: 1870 2014 4391 1870 Size: 144 2377 4545 Fragments arranged by size: 4545 2377 144 XmnI GAAnn'nnTTC Cuts at: 3231 5195 3231 Size: 1964 5102 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4874 276 Size: 53 83 186 936 3340 2468 Fragments arranged by size: 3340 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI