Restriction map for
pFN21AE1075
(Linear) (Six-base) MAPSORT of: KIEE1075.seq Check: 7376 from: 1 to: 2361
KIEE1075.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2135 2361
Size: 2135 226
AflIII A'CryG_T
Cuts at: 0 850 2361
Size: 850 1511
AhdI GACnn_n'nnGTC
Cuts at: 0 12 2361
Size: 12 2349
AleI CACnn'nnGTG
Cuts at: 0 988 2361
Size: 988 1373
AlwNI CAG_nnn'CTG
Cuts at: 0 1024 2361
Size: 1024 1337
ApaLI G'TGCA_C
Cuts at: 0 1005 2361
Size: 1005 1356
ApoI r'AATT_y
Cuts at: 0 653 1146 1224 1337 1603 1695 1789 1979
Size: 653 493 78 113 266 92 94 190
Cuts at: 1979 2017 2208 2361
Size: 38 191 153
Fragments arranged by size:
653 493 266 191 190 153 113 94
92 78 38
AseI AT'TA_AT
Cuts at: 0 1310 1559 2361
Size: 1310 249 802
Fragments arranged by size:
1310 802 249
AvaI C'yCGr_G
Cuts at: 0 9 2361
Size: 9 2352
AvrII C'CTAG_G
Cuts at: 0 1804 2361
Size: 1804 557
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2285 2318 2361
Size: 2285 33 43
Fragments arranged by size:
2285 43 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 89 2316 2361
Size: 89 2227 45
Fragments arranged by size:
2227 89 45
BclI T'GATC_A
Cuts at: 0 1667 1742 2361
Size: 1667 75 619
Fragments arranged by size:
1667 619 75
BfrBI ATG'CAT
Cuts at: 0 1492 1512 2361
Size: 1492 20 849
Fragments arranged by size:
1492 849 20
Bme1580I G_kGCm'C
Cuts at: 0 1009 2361
Size: 1009 1352
BmrI ACTGGGnnnn_n'
Cuts at: 0 2224 2361
Size: 2224 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 985 1220 2361
Size: 23 962 235 1141
Fragments arranged by size:
1141 962 235 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 111 2361
Size: 111 2250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 2361
Size: 1010 1351
BsiHKAI G_wGCw'C
Cuts at: 0 1009 2361
Size: 1009 1352
BsmI GAATG_Cn'
Cuts at: 0 936 2361
Size: 936 1425
Bsp1286I G_dGCh'C
Cuts at: 0 1009 2361
Size: 1009 1352
BsrDI GCAATG_nn'
Cuts at: 0 2096 2361
Size: 2096 265
BsrGI T'GTAC_A
Cuts at: 0 2152 2361
Size: 2152 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 407 2361
Size: 407 1954
Bsu36I CC'TnA_GG
Cuts at: 0 50 2361
Size: 50 2311
BtgI C'CryG_G
Cuts at: 0 645 1939 2361
Size: 645 1294 422
Fragments arranged by size:
1294 645 422
BtsI GCAGTG_nn'
Cuts at: 0 288 2361
Size: 288 2073
ClaI AT'CG_AT
Cuts at: 0 137 1263 2361
Size: 137 1126 1098
Fragments arranged by size:
1126 1098 137
DraI TTT'AAA
Cuts at: 0 236 845 1052 1109 1256 1319 1700 1761
Size: 236 609 207 57 147 63 381 61
Cuts at: 1761 1985 2361
Size: 224 376
Fragments arranged by size:
609 381 376 236 224 207 147 63
61 57
EarI CTCTTCn'nnn_
Cuts at: 0 920 1537 1805 2361
Size: 920 617 268 556
Fragments arranged by size:
920 617 556 268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 985 1220 2135 2361
Size: 23 962 235 915 226
Fragments arranged by size:
962 915 235 226 23
HaeII r_GCGC'y
Cuts at: 0 173 2361
Size: 173 2188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 270 302 946 978 1276 1308 2173
Size: 30 240 32 644 32 298 32 865
Cuts at: 2173 2205 2361
Size: 32 156
Fragments arranged by size:
865 644 298 240 156 32 32 32
32 30
HincII GTy'rAC
Cuts at: 0 65 1067 2315 2361
Size: 65 1002 1248 46
Fragments arranged by size:
1248 1002 65 46
HindIII A'AGCT_T
Cuts at: 0 599 1378 2361
Size: 599 779 983
Fragments arranged by size:
983 779 599
MfeI C'AATT_G
Cuts at: 0 1270 2338 2361
Size: 1270 1068 23
Fragments arranged by size:
1270 1068 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 2361
Size: 388 1973
MslI CAynn'nnrTG
Cuts at: 0 533 988 2361
Size: 533 455 1373
Fragments arranged by size:
1373 533 455
MspA1I CmG'CkG
Cuts at: 0 43 2361
Size: 43 2318
NcoI C'CATG_G
Cuts at: 0 645 1939 2361
Size: 645 1294 422
Fragments arranged by size:
1294 645 422
NsiI A_TGCA'T
Cuts at: 0 1494 1514 2361
Size: 1494 20 847
Fragments arranged by size:
1494 847 20
NspI r_CATG'y
Cuts at: 0 532 1460 1492 2361
Size: 532 928 32 869
Fragments arranged by size:
928 869 532 32
PflMI CCAn_nnn'nTGG
Cuts at: 0 548 2283 2361
Size: 548 1735 78
Fragments arranged by size:
1735 548 78
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 474 506 2361
Size: 474 32 1855
Fragments arranged by size:
1855 474 32
PstI C_TGCA'G
Cuts at: 0 295 2361
Size: 295 2066
PvuII CAG'CTG
Cuts at: 0 43 2361
Size: 43 2318
SapI GCTCTTCn'nnn_
Cuts at: 0 1805 2361
Size: 1805 556
ScaI AGT'ACT
Cuts at: 0 1613 2361
Size: 1613 748
SfcI C'TryA_G
Cuts at: 0 291 965 1070 1391 2288 2361
Size: 291 674 105 321 897 73
Fragments arranged by size:
897 674 321 291 105 73
SmaI CCC'GGG
Cuts at: 0 11 2361
Size: 11 2350
SphI G_CATG'C
Cuts at: 0 1492 2361
Size: 1492 869
SspI AAT'ATT
Cuts at: 0 726 918 1135 1325 1647 2361
Size: 726 192 217 190 322 714
Fragments arranged by size:
726 714 322 217 192 190
StyI C'CwwG_G
Cuts at: 0 542 645 1804 1939 2361
Size: 542 103 1159 135 422
Fragments arranged by size:
1159 542 422 135 103
TatI w'GTAC_w
Cuts at: 0 1611 2152 2361
Size: 1611 541 209
Fragments arranged by size:
1611 541 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1935 1967 2361
Size: 1935 32 394
Fragments arranged by size:
1935 394 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1686 2361
Size: 1686 675
XmaI C'CCGG_G
Cuts at: 0 9 2361
Size: 9 2352
XmnI GAAnn'nnTTC
Cuts at: 0 1226 2361
Size: 1226 1135
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApaLI ApoI AseI
AvaI AvrII BaeI BbsI BclI BfrBI Bme1580I BmrI
BpmI BsaI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrGI
BstXI Bsu36I BtgI BtsI ClaI DraI EarI Eco57MI
HaeII Hin4I HincII HindIII MfeI MmeI MslI MspA1I
NcoI NsiI NspI PflMI PsrI PstI PvuII SapI
ScaI SfcI SmaI SphI SspI StyI TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaI AscI AsiSI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgZI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HpaI KasI KpnI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI StuI SwaI TaqII Tth111I XbaI XhoI
ZraI