(Circular) (Six-base) MAPSORT of: pFN21AE1071.seq Check: 9159 from: 1 to: 7000 pFN21AE1071 7000 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4810 278 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4343 1937 Size: 2406 4594 Acc65I G'GTAC_C Cuts at: 2158 4321 2158 Size: 2163 4837 AclI AA'CG_TT Cuts at: 1563 5127 5500 1563 Size: 3564 373 3063 Fragments arranged by size: 3564 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2475 5063 6447 6715 1632 Size: 843 2588 1384 268 1917 Fragments arranged by size: 2588 1917 1384 843 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6803 AflIII A'CryG_T Cuts at: 1203 5899 6876 1203 Size: 4696 977 1327 Fragments arranged by size: 4696 1327 977 AgeI A'CCGG_T Cuts at: 5831 5831 Size: 7000 AhdI GACnn_n'nnGTC Cuts at: 5729 5729 Size: 7000 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7000 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7000 AlwNI CAG_nnn'CTG Cuts at: 3254 3379 4743 6315 3254 Size: 125 1364 1572 3939 Fragments arranged by size: 3939 1572 1364 125 ApaI G_GGCC'C Cuts at: 2254 2254 Size: 7000 ApaLI G'TGCA_C Cuts at: 2888 5057 6213 2888 Size: 2169 1156 3675 Fragments arranged by size: 3675 2169 1156 ApoI r'AATT_y Cuts at: 1493 2379 2422 2787 4144 4309 4535 4687 6796 Size: 886 43 365 1357 165 226 152 2109 Cuts at: 6796 1493 Size: 1697 Fragments arranged by size: 2109 1697 1357 886 365 226 165 152 43 AseI AT'TA_AT Cuts at: 160 1032 5554 160 Size: 872 4522 1606 Fragments arranged by size: 4522 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 2206 2314 4325 1870 Size: 73 263 108 2011 4545 Fragments arranged by size: 4545 2011 263 108 73 BamHI G'GATC_C Cuts at: 4330 4697 4330 Size: 367 6633 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2158 4321 5776 618 Size: 325 217 627 371 2163 1455 1842 Fragments arranged by size: 2163 1842 1455 627 371 325 217 BanII G_rGCy'C Cuts at: 1391 2254 4319 1391 Size: 863 2065 4072 Fragments arranged by size: 4072 2065 863 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6029 BbvCI CC'TCA_GC Cuts at: 2223 2223 Size: 7000 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5191 5225 1903 Size: 34 3254 34 3678 Fragments arranged by size: 3678 3254 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2590 4892 6102 2590 Size: 2302 1210 3488 Fragments arranged by size: 3488 2302 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7000 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5611 136 Size: 107 122 71 631 605 337 3602 1525 Fragments arranged by size: 3602 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2353 6995 2353 Size: 4642 2358 BlpI GC'TnA_GC Cuts at: 4417 4417 Size: 7000 Bme1580I G_kGCm'C Cuts at: 1790 2254 2892 4107 5061 6217 1790 Size: 464 638 1215 954 1156 2573 Fragments arranged by size: 2573 1215 1156 954 638 464 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7000 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4731 5689 442 Size: 416 53 83 399 24 3314 958 1753 Fragments arranged by size: 3314 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7000 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 2130 3765 5660 1239 Size: 870 21 1635 1895 2579 Fragments arranged by size: 2579 1895 1635 870 21 Bpu10I CC'TnA_GC Cuts at: 2223 2282 2223 Size: 59 6941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3108 3383 5125 5990 6288 3108 Size: 275 1742 865 298 3820 Fragments arranged by size: 3820 1742 865 298 275 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2733 5663 882 Size: 617 1234 2930 2219 Fragments arranged by size: 2930 2219 1234 617 BsaAI yAC'GTr Cuts at: 493 1204 4791 493 Size: 711 3587 2702 Fragments arranged by size: 3587 2702 711 BsaBI GATnn'nnATC Cuts at: 2841 3015 6994 2841 Size: 174 3979 2847 Fragments arranged by size: 3979 2847 174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4807 5189 5824 275 Size: 53 83 186 936 3274 382 635 1451 Fragments arranged by size: 3274 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5433 5831 6105 6252 6663 5433 Size: 398 274 147 411 5770 Fragments arranged by size: 5770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3763 3793 1178 Size: 30 165 30 2360 30 4385 Fragments arranged by size: 4385 2360 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2037 2251 1186 Size: 851 214 5935 Fragments arranged by size: 5935 851 214 BseYI C'CCAG_C Cuts at: 6203 6699 6203 Size: 496 6504 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3999 1163 Size: 573 2263 4164 Fragments arranged by size: 4164 2263 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4450 5211 6239 1540 Size: 275 186 2449 761 1028 2301 Fragments arranged by size: 2449 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2892 5061 5146 6217 2892 Size: 2169 85 1071 3675 Fragments arranged by size: 3675 2169 1071 85 BsmI GAATG_Cn' Cuts at: 2982 4511 4604 2982 Size: 1529 93 5378 Fragments arranged by size: 5378 1529 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2254 2892 4107 4319 5061 5146 6217 Size: 399 464 638 1215 212 742 85 1071 Cuts at: 6217 1391 Size: 2174 Fragments arranged by size: 2174 1215 1071 742 638 464 399 212 85 BspEI T'CCGG_A Cuts at: 6663 6663 Size: 7000 BspHI T'CATG_A Cuts at: 4889 6619 4889 Size: 1730 5270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4355 844 Size: 3511 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3864 4887 1394 Size: 429 2041 1023 3507 Fragments arranged by size: 3507 2041 1023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2516 2644 2846 3736 5495 5669 65 Size: 1183 1268 128 202 890 1759 174 1396 Fragments arranged by size: 1759 1396 1268 1183 890 202 174 128 BsrFI r'CCGG_y Cuts at: 1700 1810 5644 5831 1700 Size: 110 3834 187 2869 Fragments arranged by size: 3834 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7000 BssSI C'ACGA_G Cuts at: 5060 6072 5060 Size: 1012 5988 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7000 BstBI TT'CG_AA Cuts at: 5804 5804 Size: 7000 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7000 BstXI CCAn_nnnn'nTGG Cuts at: 3881 3881 Size: 7000 BstYI r'GATC_y Cuts at: 1972 2353 2953 3892 4330 4697 5082 5099 6551 Size: 381 600 939 438 367 385 17 1452 Cuts at: 6551 6637 6649 6995 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 939 600 438 385 381 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2198 3620 513 Size: 552 939 194 1422 3893 Fragments arranged by size: 3893 1422 939 552 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6261 Fragments arranged by size: 6261 683 56 BtsI GCAGTG_nn' Cuts at: 4107 4516 5310 5330 4107 Size: 409 794 20 5777 Fragments arranged by size: 5777 794 409 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6965 DraI TTT'AAA Cuts at: 2877 4304 2877 Size: 1427 5573 DraIII CAC_nnn'GTG Cuts at: 2233 2233 Size: 7000 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6007 809 Size: 507 4691 1802 Fragments arranged by size: 4691 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3623 4447 5336 Size: 54 1425 50 275 18 1793 824 889 Cuts at: 5336 6965 8 Size: 1629 43 Fragments arranged by size: 1793 1629 1425 889 824 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4447 1537 Size: 275 2635 4090 Fragments arranged by size: 4090 2635 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 3384 3710 3930 4930 1353 Size: 900 1131 326 220 1000 3423 Fragments arranged by size: 3423 1131 1000 900 326 220 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3682 5587 5961 6107 387 Size: 1064 138 2093 1905 374 146 1280 Fragments arranged by size: 2093 1905 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2130 2475 3765 5063 5660 6447 Size: 393 477 21 345 1290 1298 597 787 Cuts at: 6447 6715 1239 Size: 268 1524 Fragments arranged by size: 1524 1298 1290 787 597 477 393 345 268 21 EcoNI CCTnn'n_nnAGG Cuts at: 2258 2258 Size: 7000 EcoO109I rG'GnC_Cy Cuts at: 2136 2251 2136 Size: 115 6885 EcoRI G'AATT_C Cuts at: 4309 4687 4309 Size: 378 6622 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2372 2372 Size: 7000 FspI TGC'GCA Cuts at: 5506 5506 Size: 7000 HaeII r_GCGC'y Cuts at: 1678 4256 6147 1678 Size: 2578 1891 2531 Fragments arranged by size: 2578 2531 1891 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2945 2977 3137 3169 3178 Size: 32 135 32 1373 32 160 32 9 Cuts at: 3178 3210 3460 3492 5641 5673 5715 5747 1373 Size: 32 250 32 2149 32 42 32 2626 Fragments arranged by size: 2626 2149 1373 250 160 135 42 32 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 2481 3226 3771 4344 4590 669 Size: 988 281 543 745 545 573 246 3079 Fragments arranged by size: 3079 988 745 573 545 543 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7000 HpaI GTT'AAC Cuts at: 2481 4590 2481 Size: 2109 4891 KpnI G_GTAC'C Cuts at: 2162 4325 2162 Size: 2163 4837 MfeI C'AATT_G Cuts at: 4599 4599 Size: 7000 MluI A'CGCG_T Cuts at: 6876 6876 Size: 7000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2276 6114 6298 1305 Size: 971 3838 184 2007 Fragments arranged by size: 3838 2007 971 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3625 10 Size: 54 1425 343 1793 3385 Fragments arranged by size: 3385 1793 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2869 4958 5317 5476 518 Size: 648 1703 2089 359 159 2042 Fragments arranged by size: 2089 2042 1703 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2322 4416 5093 6241 6486 1606 Size: 716 2094 677 1148 245 2120 Fragments arranged by size: 2120 2094 1148 716 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5509 Fragments arranged by size: 5509 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7000 NotI GC'GGCC_GC Cuts at: 4447 4447 Size: 7000 NspI r_CATG'y Cuts at: 1132 1229 2922 4358 5903 1132 Size: 97 1693 1436 1545 2229 Fragments arranged by size: 2229 1693 1545 1436 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7000 PciI A'CATG_T Cuts at: 5899 5899 Size: 7000 PflMI CCAn_nnn'nTGG Cuts at: 1108 2665 1108 Size: 1557 5443 PfoI T'CCnGG_A Cuts at: 3895 3895 Size: 7000 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3703 3735 5069 5101 6582 6614 3703 Size: 32 1334 32 1481 32 4089 Fragments arranged by size: 4089 1481 1334 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2136 2136 Size: 7000 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7000 PsiI TTA'TAA Cuts at: 4570 4570 Size: 7000 PspOMI G'GGCC_C Cuts at: 2250 2250 Size: 7000 PstI C_TGCA'G Cuts at: 830 2220 2475 4352 830 Size: 1390 255 1877 3478 Fragments arranged by size: 3478 1877 1390 255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 SalI G'TCGA_C Cuts at: 1936 4342 1936 Size: 2406 4594 SanDI GG'GwC_CC Cuts at: 2136 2136 Size: 7000 SapI GCTCTTCn'nnn_ Cuts at: 3930 3930 Size: 7000 SbfI CC_TGCA'GG Cuts at: 4352 4352 Size: 7000 ScaI AGT'ACT Cuts at: 2903 5248 6960 2903 Size: 2345 1712 2943 Fragments arranged by size: 2943 2345 1712 SfcI C'TryA_G Cuts at: 826 1045 2216 2471 4348 5483 6164 6355 826 Size: 219 1171 255 1877 1135 681 191 1471 Fragments arranged by size: 1877 1471 1171 1135 681 255 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 2208 2316 4327 1872 Size: 336 108 2011 4545 Fragments arranged by size: 4545 2011 336 108 SmlI C'TyrA_G Cuts at: 820 1017 1943 3123 3362 5104 6005 6267 820 Size: 197 926 1180 239 1742 901 262 1553 Fragments arranged by size: 1742 1553 1180 926 901 262 239 197 SnaBI TAC'GTA Cuts at: 493 4791 493 Size: 4298 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 4358 1132 Size: 3226 3774 SspI AAT'ATT Cuts at: 5 52 2911 3692 4924 5 Size: 47 2859 781 1232 2081 Fragments arranged by size: 2859 2081 1232 781 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7000 StyI C'CwwG_G Cuts at: 513 1065 2004 4275 4439 513 Size: 552 939 2271 164 3074 Fragments arranged by size: 3074 2271 939 552 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2162 2360 2798 4160 5027 5044 5197 Size: 1255 582 198 438 1362 867 17 153 Cuts at: 5197 5382 5827 325 Size: 185 445 1498 Fragments arranged by size: 1498 1362 1255 867 582 445 438 198 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2901 5246 6958 Size: 274 80 33 51 1443 924 2345 1712 Cuts at: 6958 96 Size: 138 Fragments arranged by size: 2345 1712 1443 924 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6960 6992 6960 Size: 32 6968 Tth111I GACn'n_nGTC Cuts at: 1316 3107 1316 Size: 1791 5209 XbaI T'CTAG_A Cuts at: 4336 4336 Size: 7000 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4114 4114 Size: 7000 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7000 XmaI C'CCGG_G Cuts at: 1870 2206 2314 4325 1870 Size: 336 108 2011 4545 Fragments arranged by size: 4545 2011 336 108 XmnI GAAnn'nnTTC Cuts at: 4146 5129 4146 Size: 983 6017 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4808 276 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI