(Circular) (Six-base) MAPSORT of: pFN21AE1074.seq Check: 811 from: 1 to: 6835 pFN21AE1074 6835 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4645 278 Size: 53 83 186 936 3109 2468 Fragments arranged by size: 3109 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3411 4178 1937 Size: 1474 767 4594 Fragments arranged by size: 4594 1474 767 Acc65I G'GTAC_C Cuts at: 4156 4156 Size: 6835 AclI AA'CG_TT Cuts at: 1563 4962 5335 1563 Size: 3399 373 3063 Fragments arranged by size: 3399 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4898 6282 6550 1632 Size: 3266 1384 268 1917 Fragments arranged by size: 3266 1917 1384 268 AfeI AGC'GCT Cuts at: 2606 2606 Size: 6835 AflII C'TTAA_G Cuts at: 820 1017 2978 820 Size: 197 1961 4677 Fragments arranged by size: 4677 1961 197 AflIII A'CryG_T Cuts at: 1203 3045 5734 6711 1203 Size: 1842 2689 977 1327 Fragments arranged by size: 2689 1842 1327 977 AgeI A'CCGG_T Cuts at: 5666 5666 Size: 6835 AhdI GACnn_n'nnGTC Cuts at: 5564 5564 Size: 6835 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6835 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4008 1184 Size: 2824 4011 AlwNI CAG_nnn'CTG Cuts at: 3562 4578 6150 3562 Size: 1016 1572 4247 Fragments arranged by size: 4247 1572 1016 ApaLI G'TGCA_C Cuts at: 3791 4892 6048 3791 Size: 1101 1156 4578 Fragments arranged by size: 4578 1156 1101 ApoI r'AATT_y Cuts at: 1493 4144 4370 4522 6631 1493 Size: 2651 226 152 2109 1697 Fragments arranged by size: 2651 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5389 160 Size: 872 4357 1606 Fragments arranged by size: 4357 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 AvaI C'yCGr_G Cuts at: 1870 1943 4160 1870 Size: 73 2217 4545 Fragments arranged by size: 4545 2217 73 BamHI G'GATC_C Cuts at: 3343 4165 4532 3343 Size: 822 367 5646 Fragments arranged by size: 5646 822 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4156 5611 618 Size: 325 217 627 2369 1455 1842 Fragments arranged by size: 2369 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2072 4154 1391 Size: 681 2082 4072 Fragments arranged by size: 4072 2082 681 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2702 2917 4079 928 Size: 971 803 215 1162 3684 Fragments arranged by size: 3684 1162 971 803 215 BbvCI CC'TCA_GC Cuts at: 2768 2768 Size: 6835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5026 5060 1903 Size: 34 3089 34 3678 Fragments arranged by size: 3678 3089 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3628 3868 4727 5937 3628 Size: 240 859 1210 4526 Fragments arranged by size: 4526 1210 859 240 BclI T'GATC_A Cuts at: 1548 2896 3150 1548 Size: 1348 254 5233 Fragments arranged by size: 5233 1348 254 BfrBI ATG'CAT Cuts at: 2246 2519 2246 Size: 273 6562 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5446 136 Size: 107 122 71 631 605 3774 1525 Fragments arranged by size: 3774 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3926 6830 3926 Size: 2904 3931 BlpI GC'TnA_GC Cuts at: 3749 4252 3749 Size: 503 6332 Bme1580I G_kGCm'C Cuts at: 1790 3795 4896 6052 1790 Size: 2005 1101 1156 2573 Fragments arranged by size: 2573 2005 1156 1101 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6835 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4036 4566 5524 Size: 416 53 83 399 24 2619 530 958 Cuts at: 5524 442 Size: 1753 Fragments arranged by size: 2619 1753 958 530 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6835 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3011 3164 5495 1239 Size: 1772 153 2331 2579 Fragments arranged by size: 2579 2331 1772 153 Bpu10I CC'TnA_GC Cuts at: 2768 2768 Size: 6835 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3025 4960 5825 6123 3025 Size: 1935 865 298 3737 Fragments arranged by size: 3737 1935 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5498 882 Size: 617 3999 2219 Fragments arranged by size: 3999 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4626 493 Size: 711 3422 2702 Fragments arranged by size: 3422 2702 711 BsaBI GATnn'nnATC Cuts at: 6829 6829 Size: 6835 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4642 5024 5659 275 Size: 53 83 186 936 3109 382 635 1451 Fragments arranged by size: 3109 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5268 5666 5940 6087 6498 5268 Size: 398 274 147 411 5605 Fragments arranged by size: 5605 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6610 Fragments arranged by size: 6610 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6835 BseYI C'CCAG_C Cuts at: 6038 6534 6038 Size: 496 6339 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 1163 Size: 573 1041 5221 Fragments arranged by size: 5221 1041 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4285 5046 6074 1540 Size: 275 186 2284 761 1028 2301 Fragments arranged by size: 2301 2284 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3460 3795 4896 4981 6052 3460 Size: 335 1101 85 1071 4243 Fragments arranged by size: 4243 1101 1071 335 85 BsmI GAATG_Cn' Cuts at: 2211 2246 3104 3616 3997 4346 4439 2211 Size: 35 858 512 381 349 93 4607 Fragments arranged by size: 4607 858 512 381 349 93 35 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 3460 3795 4154 4896 4981 6052 Size: 399 282 1388 335 359 742 85 1071 Cuts at: 6052 1391 Size: 2174 Fragments arranged by size: 2174 1388 1071 742 399 359 335 282 85 BspEI T'CCGG_A Cuts at: 6498 6498 Size: 6835 BspHI T'CATG_A Cuts at: 2413 4724 6454 2413 Size: 2311 1730 2794 Fragments arranged by size: 2794 2311 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4190 844 Size: 3346 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4722 1394 Size: 429 2899 3507 Fragments arranged by size: 3507 2899 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 2847 4002 5330 5504 65 Size: 1183 1323 276 1155 1328 174 1396 Fragments arranged by size: 1396 1328 1323 1183 1155 276 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5479 5666 1700 Size: 110 3669 187 2869 Fragments arranged by size: 3669 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6835 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6835 BssSI C'ACGA_G Cuts at: 4895 5907 4895 Size: 1012 5823 BstAPI GCAn_nnn'nTGC Cuts at: 794 2764 2972 794 Size: 1970 208 4657 Fragments arranged by size: 4657 1970 208 BstBI TT'CG_AA Cuts at: 5639 5639 Size: 6835 BstEII G'GTnAC_C Cuts at: 1184 2773 1184 Size: 1589 5246 BstYI r'GATC_y Cuts at: 1972 3196 3343 3926 4165 4532 4917 4934 6386 Size: 1224 147 583 239 367 385 17 1452 Cuts at: 6386 6472 6484 6830 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1224 583 385 367 346 239 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3127 3701 513 Size: 552 2062 574 3647 Fragments arranged by size: 3647 2062 574 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6096 Fragments arranged by size: 6096 683 56 BtsI GCAGTG_nn' Cuts at: 4351 5145 5165 4351 Size: 794 20 6021 Fragments arranged by size: 6021 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6835 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6800 DraI TTT'AAA Cuts at: 2187 2257 4139 2187 Size: 70 1882 4883 Fragments arranged by size: 4883 1882 70 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5842 809 Size: 507 4526 1802 Fragments arranged by size: 4526 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4282 5171 6800 Size: 54 1425 50 275 18 2452 889 1629 Cuts at: 6800 8 Size: 43 Fragments arranged by size: 2452 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4282 1537 Size: 275 2470 4090 Fragments arranged by size: 4090 2470 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3206 3323 3400 4104 4765 1353 Size: 1853 117 77 704 661 3423 Fragments arranged by size: 3423 1853 704 661 117 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5422 5796 5942 387 Size: 1064 138 3833 374 146 1280 Fragments arranged by size: 3833 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3011 3164 4898 5495 6282 6550 1239 Size: 393 1379 153 1734 597 787 268 1524 Fragments arranged by size: 1734 1524 1379 787 597 393 268 153 EcoRI G'AATT_C Cuts at: 4144 4522 4144 Size: 378 6457 EcoRV GAT'ATC Cuts at: 2221 2231 2221 Size: 10 6825 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2423 2453 2423 Size: 30 6805 FspI TGC'GCA Cuts at: 5341 5341 Size: 6835 HaeII r_GCGC'y Cuts at: 1678 2534 2608 5982 1678 Size: 856 74 3374 2531 Fragments arranged by size: 3374 2531 856 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3335 3367 3454 3486 3698 Size: 32 135 32 1763 32 87 32 212 Cuts at: 3698 3730 5476 5508 5550 5582 1373 Size: 32 1746 32 42 32 2626 Fragments arranged by size: 2626 1763 1746 212 135 87 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2826 4179 4425 669 Size: 988 281 72 816 1353 246 3079 Fragments arranged by size: 3079 1353 988 816 281 246 72 HindIII A'AGCT_T Cuts at: 748 2726 748 Size: 1978 4857 HpaI GTT'AAC Cuts at: 4425 4425 Size: 6835 KpnI G_GTAC'C Cuts at: 4160 4160 Size: 6835 MfeI C'AATT_G Cuts at: 2574 4434 2574 Size: 1860 4975 MluI A'CGCG_T Cuts at: 6711 6711 Size: 6835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5949 6133 1305 Size: 4644 184 2007 Fragments arranged by size: 4644 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5013 Fragments arranged by size: 5013 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 3726 3990 4793 5152 5311 518 Size: 648 1398 1162 264 803 359 159 2042 Fragments arranged by size: 2042 1398 1162 803 648 359 264 159 MspA1I CmG'CkG Cuts at: 1606 3697 3703 4251 4928 6076 6321 1606 Size: 2091 6 548 677 1148 245 2120 Fragments arranged by size: 2120 2091 1148 677 548 245 6 NcoI C'CATG_G Cuts at: 513 1065 3127 513 Size: 552 2062 4221 Fragments arranged by size: 4221 2062 552 NdeI CA'TA_TG Cuts at: 387 2661 387 Size: 2274 4561 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6835 NotI GC'GGCC_GC Cuts at: 4282 4282 Size: 6835 NsiI A_TGCA'T Cuts at: 2248 2521 2248 Size: 273 6562 NspI r_CATG'y Cuts at: 1132 1229 3049 4193 5738 1132 Size: 97 1820 1144 1545 2229 Fragments arranged by size: 2229 1820 1545 1144 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6835 PciI A'CATG_T Cuts at: 3045 5734 3045 Size: 2689 4146 PflMI CCAn_nnn'nTGG Cuts at: 1108 3193 1108 Size: 2085 4750 PfoI T'CCnGG_A Cuts at: 3090 3090 Size: 6835 PmeI GTTT'AAAC Cuts at: 4139 4139 Size: 6835 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4904 4936 6417 6449 4904 Size: 32 1481 32 5290 Fragments arranged by size: 5290 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6835 PsiI TTA'TAA Cuts at: 4405 4405 Size: 6835 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2687 2719 2687 Size: 32 6803 PstI C_TGCA'G Cuts at: 830 4187 830 Size: 3357 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6835 PvuII CAG'CTG Cuts at: 3697 3697 Size: 6835 SacII CC_GC'GG Cuts at: 3704 3704 Size: 6835 SalI G'TCGA_C Cuts at: 1936 4177 1936 Size: 2241 4594 SapI GCTCTTCn'nnn_ Cuts at: 4104 4104 Size: 6835 SbfI CC_TGCA'GG Cuts at: 4187 4187 Size: 6835 ScaI AGT'ACT Cuts at: 3057 5083 6795 3057 Size: 2026 1712 3097 Fragments arranged by size: 3097 2026 1712 SfcI C'TryA_G Cuts at: 826 1045 3888 4183 5318 5999 6190 826 Size: 219 2843 295 1135 681 191 1471 Fragments arranged by size: 2843 1471 1135 681 295 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6835 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6835 SmaI CCC'GGG Cuts at: 1872 4162 1872 Size: 2290 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2978 3004 4939 5840 6102 820 Size: 197 926 1035 26 1935 901 262 1553 Fragments arranged by size: 1935 1553 1035 926 901 262 197 26 SnaBI TAC'GTA Cuts at: 493 4626 493 Size: 4133 2702 SpeI A'CTAG_T Cuts at: 152 2890 152 Size: 2738 4097 SphI G_CATG'C Cuts at: 1132 4193 1132 Size: 3061 3774 SspI AAT'ATT Cuts at: 5 52 4759 5 Size: 47 4707 2081 Fragments arranged by size: 4707 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6835 StyI C'CwwG_G Cuts at: 513 1065 2442 3127 3289 4274 513 Size: 552 1377 685 162 985 3074 Fragments arranged by size: 3074 1377 985 685 552 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3120 4862 4879 5032 5217 5662 325 Size: 1255 1540 1742 17 153 185 445 1498 Fragments arranged by size: 1742 1540 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2099 3055 5081 Size: 274 80 33 51 1443 122 956 2026 Cuts at: 5081 6793 96 Size: 1712 138 Fragments arranged by size: 2026 1712 1443 956 274 138 122 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6795 6827 6795 Size: 32 6803 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6835 XbaI T'CTAG_A Cuts at: 4171 4171 Size: 6835 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6835 XmaI C'CCGG_G Cuts at: 1870 4160 1870 Size: 2290 4545 XmnI GAAnn'nnTTC Cuts at: 2035 2433 4098 4964 2035 Size: 398 1665 866 3906 Fragments arranged by size: 3906 1665 866 398 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4643 276 Size: 53 83 186 936 3109 2468 Fragments arranged by size: 3109 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI