Restriction map for
pFN21AE1590
(Linear) (Six-base) MAPSORT of: KIEE1590.seq Check: 6406 from: 1 to: 3504
KIEE1590.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3294 3504
Size: 3294 210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 914 1752 2287 2959 3394 3504
Size: 765 149 838 535 672 435 110
Fragments arranged by size:
838 765 672 535 435 149 110
AflIII A'CryG_T
Cuts at: 0 500 981 1049 3504
Size: 500 481 68 2455
Fragments arranged by size:
2455 500 481 68
AleI CACnn'nnGTG
Cuts at: 0 2417 3504
Size: 2417 1087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 709 3504
Size: 709 2795
AlwNI CAG_nnn'CTG
Cuts at: 0 2196 3504
Size: 2196 1308
ApaI G_GGCC'C
Cuts at: 0 2114 3504
Size: 2114 1390
ApaLI G'TGCA_C
Cuts at: 0 1059 3477 3504
Size: 1059 2418 27
Fragments arranged by size:
2418 1059 27
ApoI r'AATT_y
Cuts at: 0 942 1215 2431 3504
Size: 942 273 1216 1073
Fragments arranged by size:
1216 1073 942 273
AvaI C'yCGr_G
Cuts at: 0 1538 1832 1842 2820 3487 3504
Size: 1538 294 10 978 667 17
Fragments arranged by size:
1538 978 667 294 17 10
BamHI G'GATC_C
Cuts at: 0 255 3320 3504
Size: 255 3065 184
Fragments arranged by size:
3065 255 184
BanI G'GyrC_C
Cuts at: 0 1143 3504
Size: 1143 2361
BanII G_rGCy'C
Cuts at: 0 82 634 1675 2114 2159 3458 3494 3504
Size: 82 552 1041 439 45 1299 36 10
Fragments arranged by size:
1299 1041 552 439 82 45 36 10
BbeI G_GCGC'C
Cuts at: 0 1147 3504
Size: 1147 2357
BbsI GAAGACnn'nnnn_
Cuts at: 0 150 365 1007 1969 3504
Size: 150 215 642 962 1535
Fragments arranged by size:
1535 962 642 215 150
BbvCI CC'TCA_GC
Cuts at: 0 134 293 3504
Size: 134 159 3211
Fragments arranged by size:
3211 159 134
BclI T'GATC_A
Cuts at: 0 405 3504
Size: 405 3099
BglII A'GATC_T
Cuts at: 0 1504 3504
Size: 1504 2000
BlpI GC'TnA_GC
Cuts at: 0 2332 2441 3504
Size: 2332 109 1063
Fragments arranged by size:
2332 1063 109
Bme1580I G_kGCm'C
Cuts at: 0 1063 2114 3481 3504
Size: 1063 1051 1367 23
Fragments arranged by size:
1367 1063 1051 23
BmgBI CAC'GTC
Cuts at: 0 606 2407 3186 3504
Size: 606 1801 779 318
Fragments arranged by size:
1801 779 606 318
BmrI ACTGGGnnnn_n'
Cuts at: 0 1451 3504
Size: 1451 2053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2001 2324 2884 3421 3504
Size: 2001 323 560 537 83
Fragments arranged by size:
2001 560 537 323 83
Bpu10I CC'TnA_GC
Cuts at: 0 134 230 293 1139 3355 3504
Size: 134 96 63 846 2216 149
Fragments arranged by size:
2216 846 149 134 96 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2722 3352 3504
Size: 2722 630 152
Fragments arranged by size:
2722 630 152
BsaI GGTCTCn'nnnn_
Cuts at: 0 54 1302 2680 2840 3504
Size: 54 1248 1378 160 664
Fragments arranged by size:
1378 1248 664 160 54
BsaAI yAC'GTr
Cuts at: 0 984 1050 3504
Size: 984 66 2454
Fragments arranged by size:
2454 984 66
BsaHI Gr'CG_yC
Cuts at: 0 1144 1894 3504
Size: 1144 750 1610
Fragments arranged by size:
1610 1144 750
BsaWI w'CCGG_w
Cuts at: 0 1018 3504
Size: 1018 2486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1690 1720 3460 3490 3504
Size: 1690 30 1740 30 14
Fragments arranged by size:
1740 1690 30 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 808 1675 1683 2881 3206 3445 3504
Size: 484 324 867 8 1198 325 239 59
Fragments arranged by size:
1198 867 484 325 324 239 59 8
BseYI C'CCAG_C
Cuts at: 0 710 1151 1757 2061 2470 2528 2591 2884
Size: 710 441 606 304 409 58 63 293
Cuts at: 2884 2937 3457 3504
Size: 53 520 47
Fragments arranged by size:
710 606 520 441 409 304 293 63
58 53 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 859 1147 1168 3504
Size: 460 399 288 21 2336
Fragments arranged by size:
2336 460 399 288 21
BsiEI CG_ry'CG
Cuts at: 0 537 3409 3465 3504
Size: 537 2872 56 39
Fragments arranged by size:
2872 537 56 39
BsiHKAI G_wGCw'C
Cuts at: 0 527 1063 1675 1888 2159 2873 3481 3504
Size: 527 536 612 213 271 714 608 23
Fragments arranged by size:
714 612 608 536 527 271 213 23
BsmI GAATG_Cn'
Cuts at: 0 93 1813 1974 3504
Size: 93 1720 161 1530
Fragments arranged by size:
1720 1530 161 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1885 3504
Size: 1885 1619
Bsp1286I G_dGCh'C
Cuts at: 0 82 527 634 1063 1675 1888 2114 2159
Size: 82 445 107 429 612 213 226 45
Cuts at: 2159 2873 3458 3481 3494 3504
Size: 714 585 23 13 10
Fragments arranged by size:
714 612 585 445 429 226 213 107
82 45 23 13 10
BspEI T'CCGG_A
Cuts at: 0 1018 3504
Size: 1018 2486
BspHI T'CATG_A
Cuts at: 0 324 1899 2832 3504
Size: 324 1575 933 672
Fragments arranged by size:
1575 933 672 324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1836 3294 3504
Size: 1836 1458 210
Fragments arranged by size:
1836 1458 210
BsrBI CCG'CTC
Cuts at: 0 3405 3504
Size: 3405 99
BsrFI r'CCGG_y
Cuts at: 0 371 567 3504
Size: 371 196 2937
Fragments arranged by size:
2937 371 196
BssSI C'ACGA_G
Cuts at: 0 2150 2872 3504
Size: 2150 722 632
Fragments arranged by size:
2150 722 632
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1182 2400 3384 3504
Size: 1182 1218 984 120
Fragments arranged by size:
1218 1182 984 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 679 1581 3504
Size: 679 902 1923
Fragments arranged by size:
1923 902 679
BstYI r'GATC_y
Cuts at: 0 255 1504 1584 2801 3320 3504
Size: 255 1249 80 1217 519 184
Fragments arranged by size:
1249 1217 519 255 184 80
BtgI C'CryG_G
Cuts at: 0 3494 3504
Size: 3494 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1400 3132 3504
Size: 1400 1732 372
Fragments arranged by size:
1732 1400 372
BtsI GCAGTG_nn'
Cuts at: 0 477 1130 3366 3504
Size: 477 653 2236 138
Fragments arranged by size:
2236 653 477 138
ClaI AT'CG_AT
Cuts at: 0 3206 3504
Size: 3206 298
DraI TTT'AAA
Cuts at: 0 2730 2997 3504
Size: 2730 267 507
Fragments arranged by size:
2730 507 267
DraIII CAC_nnn'GTG
Cuts at: 0 1459 1881 3504
Size: 1459 422 1623
Fragments arranged by size:
1623 1459 422
EaeI y'GGCC_r
Cuts at: 0 534 885 1118 2779 3504
Size: 534 351 233 1661 725
Fragments arranged by size:
1661 725 534 351 233
EagI C'GGCC_G
Cuts at: 0 534 3504
Size: 534 2970
EarI CTCTTCn'nnn_
Cuts at: 0 532 880 1286 1364 1730 2714 3504
Size: 532 348 406 78 366 984 790
Fragments arranged by size:
984 790 532 406 366 348 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 554 3459 3504
Size: 554 2905 45
Fragments arranged by size:
2905 554 45
EcoICRI GAG'CTC
Cuts at: 0 1673 2157 3504
Size: 1673 484 1347
Fragments arranged by size:
1673 1347 484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 914 1752 2001 2287 2324 2884 2959
Size: 765 149 838 249 286 37 560 75
Cuts at: 2959 3394 3421 3504
Size: 435 27 83
Fragments arranged by size:
838 765 560 435 286 249 149 83
75 37 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 233 1102 1770 3504
Size: 233 869 668 1734
Fragments arranged by size:
1734 869 668 233
EcoO109I rG'GnC_Cy
Cuts at: 0 759 1701 1838 2111 3504
Size: 759 942 137 273 1393
Fragments arranged by size:
1393 942 759 273 137
EcoRI G'AATT_C
Cuts at: 0 1215 3504
Size: 1215 2289
EcoRV GAT'ATC
Cuts at: 0 852 956 3504
Size: 852 104 2548
Fragments arranged by size:
2548 852 104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 211 1671 3217 3504
Size: 211 1460 1546 287
Fragments arranged by size:
1546 1460 287 211
FspI TGC'GCA
Cuts at: 0 697 3504
Size: 697 2807
HaeII r_GCGC'y
Cuts at: 0 1147 2265 3504
Size: 1147 1118 1239
Fragments arranged by size:
1239 1147 1118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1690 1722 1903 1935 2408 2440 3111 3143
Size: 1690 32 181 32 473 32 671 32
Cuts at: 3143 3504
Size: 361
Fragments arranged by size:
1690 671 473 361 181 32 32 32
32
HincII GTy'rAC
Cuts at: 0 247 3069 3504
Size: 247 2822 435
Fragments arranged by size:
2822 435 247
HindIII A'AGCT_T
Cuts at: 0 3164 3504
Size: 3164 340
KasI G'GCGC_C
Cuts at: 0 1143 3504
Size: 1143 2361
MscI TGG'CCA
Cuts at: 0 887 3504
Size: 887 2617
MslI CAynn'nnrTG
Cuts at: 0 112 1579 1638 1937 2417 2711 3504
Size: 112 1467 59 299 480 294 793
Fragments arranged by size:
1467 793 480 299 294 112 59
MspA1I CmG'CkG
Cuts at: 0 72 611 773 1155 1749 2520 2937 3504
Size: 72 539 162 382 594 771 417 567
Fragments arranged by size:
771 594 567 539 417 382 162 72
NaeI GCC'GGC
Cuts at: 0 569 3504
Size: 569 2935
NarI GG'CG_CC
Cuts at: 0 1144 3504
Size: 1144 2360
NdeI CA'TA_TG
Cuts at: 0 2708 3504
Size: 2708 796
NgoMIV G'CCGG_C
Cuts at: 0 567 3504
Size: 567 2937
NotI GC'GGCC_GC
Cuts at: 0 534 3504
Size: 534 2970
NspI r_CATG'y
Cuts at: 0 2539 3504
Size: 2539 965
PasI CC'CwG_GG
Cuts at: 0 122 3504
Size: 122 3382
PflMI CCAn_nnn'nTGG
Cuts at: 0 2658 3237 3504
Size: 2658 579 267
Fragments arranged by size:
2658 579 267
PfoI T'CCnGG_A
Cuts at: 0 1978 2340 3504
Size: 1978 362 1164
Fragments arranged by size:
1978 1164 362
PmlI CAC'GTG
Cuts at: 0 984 3504
Size: 984 2520
PpuMI rG'GwC_Cy
Cuts at: 0 1701 1838 3504
Size: 1701 137 1666
Fragments arranged by size:
1701 1666 137
PsiI TTA'TAA
Cuts at: 0 3076 3504
Size: 3076 428
PspOMI G'GGCC_C
Cuts at: 0 2110 3504
Size: 2110 1394
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2553 2585 3504
Size: 2553 32 919
Fragments arranged by size:
2553 919 32
PstI C_TGCA'G
Cuts at: 0 71 752 1565 3044 3422 3504
Size: 71 681 813 1479 378 82
Fragments arranged by size:
1479 813 681 378 82 71
PvuII CAG'CTG
Cuts at: 0 72 1155 2520 3504
Size: 72 1083 1365 984
Fragments arranged by size:
1365 1083 984 72
SacI G_AGCT'C
Cuts at: 0 1675 2159 3504
Size: 1675 484 1345
Fragments arranged by size:
1675 1345 484
SanDI GG'GwC_CC
Cuts at: 0 1838 3504
Size: 1838 1666
SapI GCTCTTCn'nnn_
Cuts at: 0 532 880 1730 3504
Size: 532 348 850 1774
Fragments arranged by size:
1774 850 532 348
SbfI CC_TGCA'GG
Cuts at: 0 1565 3504
Size: 1565 1939
SfcI C'TryA_G
Cuts at: 0 67 748 1561 2312 3040 3418 3504
Size: 67 681 813 751 728 378 86
Fragments arranged by size:
813 751 728 681 378 86 67
SfoI GGC'GCC
Cuts at: 0 1145 3504
Size: 1145 2359
SmaI CCC'GGG
Cuts at: 0 1540 1834 1844 3504
Size: 1540 294 10 1660
Fragments arranged by size:
1660 1540 294 10
SmlI C'TyrA_G
Cuts at: 0 2737 2820 3331 3504
Size: 2737 83 511 173
Fragments arranged by size:
2737 511 173 83
SphI G_CATG'C
Cuts at: 0 2539 3504
Size: 2539 965
SspI AAT'ATT
Cuts at: 0 834 1437 3504
Size: 834 603 2067
Fragments arranged by size:
2067 834 603
StuI AGG'CCT
Cuts at: 0 1108 1559 1654 1765 2635 3504
Size: 1108 451 95 111 870 869
Fragments arranged by size:
1108 870 869 451 111 95
StyI C'CwwG_G
Cuts at: 0 2553 2670 3096 3504
Size: 2553 117 426 408
Fragments arranged by size:
2553 426 408 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1326 1470 2591 3504
Size: 1326 144 1121 913
Fragments arranged by size:
1326 1121 913 144
TatI w'GTAC_w
Cuts at: 0 1606 3504
Size: 1606 1898
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 469 501 2136 2168 3460 3492 3504
Size: 469 32 1635 32 1292 32 12
Fragments arranged by size:
1635 1292 469 32 32 32 12
Tth111I GACn'n_nGTC
Cuts at: 0 364 3504
Size: 364 3140
XbaI T'CTAG_A
Cuts at: 0 2646 3504
Size: 2646 858
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3099 3504
Size: 3099 405
XhoI C'TCGA_G
Cuts at: 0 2820 3504
Size: 2820 684
XmaI C'CCGG_G
Cuts at: 0 1538 1832 1842 3504
Size: 1538 294 10 1662
Fragments arranged by size:
1662 1538 294 10
XmnI GAAnn'nnTTC
Cuts at: 0 1215 1295 3504
Size: 1215 80 2209
Fragments arranged by size:
2209 1215 80
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BclI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NotI NspI PasI
PflMI PfoI PmlI PpuMI PsiI PspOMI PsrI PstI
PvuII SacI SanDI SapI SbfI SfcI SfoI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BfrBI BglI BmtI BplI BsaBI BsiWI BsrDI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I CspCI DrdI FseI
FspAI HpaI KpnI MfeI MluI MmeI NcoI NheI
NruI NsiI PacI PciI PmeI PpiI PshAI PspXI
PvuI RsrII SacII SalI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SwaI ZraI