Restriction map for
pFN21AE1097
(Linear) (Six-base) MAPSORT of: KIEE1097.seq Check: 3078 from: 1 to: 1818
KIEE1097.orf
With 182 enzymes: SgfI *
November 19, 2009 09:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 768 967 1233 1818
Size: 637 131 199 266 585
Fragments arranged by size:
637 585 266 199 131
AfeI AGC'GCT
Cuts at: 0 1246 1818
Size: 1246 572
AleI CACnn'nnGTG
Cuts at: 0 160 1818
Size: 160 1658
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1742 1774 1818
Size: 1742 32 44
Fragments arranged by size:
1742 44 32
AlwNI CAG_nnn'CTG
Cuts at: 0 131 321 423 552 1818
Size: 131 190 102 129 1266
Fragments arranged by size:
1266 190 131 129 102
ApaI G_GGCC'C
Cuts at: 0 119 1471 1534 1634 1652 1670 1818
Size: 119 1352 63 100 18 18 148
Fragments arranged by size:
1352 148 119 100 63 18 18
ApaLI G'TGCA_C
Cuts at: 0 658 1111 1818
Size: 658 453 707
Fragments arranged by size:
707 658 453
ApoI r'AATT_y
Cuts at: 0 413 1818
Size: 413 1405
AvaI C'yCGr_G
Cuts at: 0 1544 1653 1779 1818
Size: 1544 109 126 39
Fragments arranged by size:
1544 126 109 39
BamHI G'GATC_C
Cuts at: 0 324 698 707 1757 1818
Size: 324 374 9 1050 61
Fragments arranged by size:
1050 374 324 61 9
BanI G'GyrC_C
Cuts at: 0 1072 1222 1369 1818
Size: 1072 150 147 449
Fragments arranged by size:
1072 449 150 147
BanII G_rGCy'C
Cuts at: 0 119 560 1187 1471 1534 1634 1652 1670
Size: 119 441 627 284 63 100 18 18
Cuts at: 1670 1818
Size: 148
Fragments arranged by size:
627 441 284 148 119 100 63 18
18
BbsI GAAGACnn'nnnn_
Cuts at: 0 260 724 1711 1818
Size: 260 464 987 107
Fragments arranged by size:
987 464 260 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 13 47 1818
Size: 13 34 1771
Fragments arranged by size:
1771 34 13
BfrBI ATG'CAT
Cuts at: 0 975 1818
Size: 975 843
BglI GCCn_nnn'nGGC
Cuts at: 0 1538 1565 1638 1647 1674 1818
Size: 1538 27 73 9 27 144
Fragments arranged by size:
1538 144 73 27 27 9
Bme1580I G_kGCm'C
Cuts at: 0 119 662 1115 1225 1266 1374 1471 1534
Size: 119 543 453 110 41 108 97 63
Cuts at: 1534 1634 1652 1670 1818
Size: 100 18 18 148
Fragments arranged by size:
543 453 148 119 110 108 100 97
63 41 18 18
BmgBI CAC'GTC
Cuts at: 0 1140 1818
Size: 1140 678
BmrI ACTGGGnnnn_n'
Cuts at: 0 264 1818
Size: 264 1554
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 495 1818
Size: 495 1323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 427 727 836 889 1367 1523 1610
Size: 298 129 300 109 53 478 156 87
Cuts at: 1610 1646 1772 1818
Size: 36 126 46
Fragments arranged by size:
478 300 298 156 129 126 109 87
53 46 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1818
Size: 1108 710
BsaI GGTCTCn'nnnn_
Cuts at: 0 724 1445 1664 1780 1818
Size: 724 721 219 116 38
Fragments arranged by size:
724 721 219 116 38
BsaAI yAC'GTr
Cuts at: 0 1110 1818
Size: 1110 708
BsaWI w'CCGG_w
Cuts at: 0 1283 1818
Size: 1283 535
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 809 839 1818
Size: 232 30 547 30 979
Fragments arranged by size:
979 547 232 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 742 846 1195 1321 1818
Size: 415 327 104 349 126 497
Fragments arranged by size:
497 415 349 327 126 104
BseYI C'CCAG_C
Cuts at: 0 364 372 1158 1218 1818
Size: 364 8 786 60 600
Fragments arranged by size:
786 600 364 60 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1137 1818
Size: 463 674 681
Fragments arranged by size:
681 674 463
BsiEI CG_ry'CG
Cuts at: 0 17 1818
Size: 17 1801
BsiHKAI G_wGCw'C
Cuts at: 0 479 662 1115 1187 1818
Size: 479 183 453 72 631
Fragments arranged by size:
631 479 453 183 72
Bsp1286I G_dGCh'C
Cuts at: 0 119 479 560 662 1115 1187 1225 1266
Size: 119 360 81 102 453 72 38 41
Cuts at: 1266 1374 1471 1534 1634 1652 1670 1818
Size: 108 97 63 100 18 18 148
Fragments arranged by size:
453 360 148 119 108 102 100 97
81 72 63 41 38 18 18
BspEI T'CCGG_A
Cuts at: 0 1283 1818
Size: 1283 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1241 1818
Size: 1241 577
BsrDI GCAATG_nn'
Cuts at: 0 1356 1818
Size: 1356 462
BsrFI r'CCGG_y
Cuts at: 0 16 107 1818
Size: 16 91 1711
Fragments arranged by size:
1711 91 16
BssSI C'ACGA_G
Cuts at: 0 1057 1818
Size: 1057 761
BstAPI GCAn_nnn'nTGC
Cuts at: 0 131 1119 1818
Size: 131 988 699
Fragments arranged by size:
988 699 131
BstEII G'GTnAC_C
Cuts at: 0 1712 1818
Size: 1712 106
BstXI CCAn_nnnn'nTGG
Cuts at: 0 364 1218 1302 1818
Size: 364 854 84 516
Fragments arranged by size:
854 516 364 84
BstYI r'GATC_y
Cuts at: 0 324 383 698 707 1757 1818
Size: 324 59 315 9 1050 61
Fragments arranged by size:
1050 324 315 61 59 9
Bsu36I CC'TnA_GG
Cuts at: 0 1490 1671 1679 1742 1818
Size: 1490 181 8 63 76
Fragments arranged by size:
1490 181 76 63 8
BtgI C'CryG_G
Cuts at: 0 203 677 702 1733 1818
Size: 203 474 25 1031 85
Fragments arranged by size:
1031 474 203 85 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 38 1818
Size: 38 1780
BtsI GCAGTG_nn'
Cuts at: 0 662 1325 1818
Size: 662 663 493
Fragments arranged by size:
663 662 493
DrdI GACnn_nn'nnGTC
Cuts at: 0 1279 1484 1818
Size: 1279 205 334
Fragments arranged by size:
1279 334 205
EaeI y'GGCC_r
Cuts at: 0 14 109 200 1818
Size: 14 95 91 1618
Fragments arranged by size:
1618 95 91 14
EagI C'GGCC_G
Cuts at: 0 14 1818
Size: 14 1804
EarI CTCTTCn'nnn_
Cuts at: 0 44 184 565 787 959 1818
Size: 44 140 381 222 172 859
Fragments arranged by size:
859 381 222 172 140 44
EcoICRI GAG'CTC
Cuts at: 0 1185 1818
Size: 1185 633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 427 637 727 768 836 889 967
Size: 298 129 210 90 41 68 53 78
Cuts at: 967 1233 1367 1523 1610 1646 1772 1818
Size: 266 134 156 87 36 126 46
Fragments arranged by size:
298 266 210 156 134 129 126 90
87 78 68 53 46 41 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 628 1818
Size: 568 60 1190
Fragments arranged by size:
1190 568 60
EcoO109I rG'GnC_Cy
Cuts at: 0 54 115 116 318 621 851 1423 1468
Size: 54 61 1 202 303 230 572 45
Cuts at: 1468 1477 1486 1530 1531 1630 1631 1648 1649
Size: 9 9 44 1 99 1 17 1
Cuts at: 1649 1666 1667 1675 1738 1765 1783 1818
Size: 17 1 8 63 27 18 35
Fragments arranged by size:
572 303 230 202 99 63 61 54
45 44 35 27 18 17 17 9
9 8 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 413 1818
Size: 413 1405
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1596 1818
Size: 1596 222
FseI GG_CCGG'CC
Cuts at: 0 111 1818
Size: 111 1707
FspI TGC'GCA
Cuts at: 0 796 1818
Size: 796 1022
HaeII r_GCGC'y
Cuts at: 0 1248 1818
Size: 1248 570
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 809 841 925 957 1749 1781
Size: 232 32 545 32 84 32 792 32
Cuts at: 1781 1818
Size: 37
Fragments arranged by size:
792 545 232 84 37 32 32 32
32
HincII GTy'rAC
Cuts at: 0 543 1818
Size: 543 1275
HindIII A'AGCT_T
Cuts at: 0 289 1818
Size: 289 1529
HpaI GTT'AAC
Cuts at: 0 543 1818
Size: 543 1275
MslI CAynn'nnrTG
Cuts at: 0 160 1172 1300 1818
Size: 160 1012 128 518
Fragments arranged by size:
1012 518 160 128
MspA1I CmG'CkG
Cuts at: 0 122 131 372 447 598 714 788 924
Size: 122 9 241 75 151 116 74 136
Cuts at: 924 930 1725 1818
Size: 6 795 93
Fragments arranged by size:
795 241 151 136 122 116 93 75
74 9 6
NaeI GCC'GGC
Cuts at: 0 18 109 1818
Size: 18 91 1709
Fragments arranged by size:
1709 91 18
NcoI C'CATG_G
Cuts at: 0 1733 1818
Size: 1733 85
NgoMIV G'CCGG_C
Cuts at: 0 16 107 1818
Size: 16 91 1711
Fragments arranged by size:
1711 91 16
NsiI A_TGCA'T
Cuts at: 0 977 1818
Size: 977 841
NspI r_CATG'y
Cuts at: 0 81 891 975 1122 1818
Size: 81 810 84 147 696
Fragments arranged by size:
810 696 147 84 81
PasI CC'CwG_GG
Cuts at: 0 846 1473 1482 1636 1818
Size: 846 627 9 154 182
Fragments arranged by size:
846 627 182 154 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 510 552 912 1818
Size: 321 189 42 360 906
Fragments arranged by size:
906 360 321 189 42
PfoI T'CCnGG_A
Cuts at: 0 950 1238 1383 1818
Size: 950 288 145 435
Fragments arranged by size:
950 435 288 145
PmlI CAC'GTG
Cuts at: 0 1110 1818
Size: 1110 708
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 1818
Size: 331 32 1455
Fragments arranged by size:
1455 331 32
PpuMI rG'GwC_Cy
Cuts at: 0 54 621 1477 1486 1765 1818
Size: 54 567 856 9 279 53
Fragments arranged by size:
856 567 279 54 53 9
PspOMI G'GGCC_C
Cuts at: 0 115 1467 1530 1630 1648 1666 1818
Size: 115 1352 63 100 18 18 152
Fragments arranged by size:
1352 152 115 100 63 18 18
PspXI vC'TCGA_Gb
Cuts at: 0 1544 1818
Size: 1544 274
PstI C_TGCA'G
Cuts at: 0 127 398 657 929 1818
Size: 127 271 259 272 889
Fragments arranged by size:
889 272 271 259 127
PvuII CAG'CTG
Cuts at: 0 372 447 598 924 930 1818
Size: 372 75 151 326 6 888
Fragments arranged by size:
888 372 326 151 75 6
SacI G_AGCT'C
Cuts at: 0 1187 1818
Size: 1187 631
SanDI GG'GwC_CC
Cuts at: 0 1477 1765 1818
Size: 1477 288 53
Fragments arranged by size:
1477 288 53
SapI GCTCTTCn'nnn_
Cuts at: 0 565 787 1818
Size: 565 222 1031
Fragments arranged by size:
1031 565 222
SexAI A'CCwGG_T
Cuts at: 0 536 896 1818
Size: 536 360 922
Fragments arranged by size:
922 536 360
SfcI C'TryA_G
Cuts at: 0 123 394 653 925 1818
Size: 123 271 259 272 893
Fragments arranged by size:
893 272 271 259 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1538 1638 1647 1674 1818
Size: 1538 100 9 27 144
Fragments arranged by size:
1538 144 100 27 9
SmaI CCC'GGG
Cuts at: 0 1655 1781 1818
Size: 1655 126 37
Fragments arranged by size:
1655 126 37
SmlI C'TyrA_G
Cuts at: 0 1123 1544 1818
Size: 1123 421 274
Fragments arranged by size:
1123 421 274
SphI G_CATG'C
Cuts at: 0 891 1122 1818
Size: 891 231 696
Fragments arranged by size:
891 696 231
StuI AGG'CCT
Cuts at: 0 1559 1818
Size: 1559 259
StyI C'CwwG_G
Cuts at: 0 1733 1818
Size: 1733 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 643 1186 1818
Size: 112 531 543 632
Fragments arranged by size:
632 543 531 112
Tth111I GACn'n_nGTC
Cuts at: 0 270 1710 1818
Size: 270 1440 108
Fragments arranged by size:
1440 270 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 1768 1818
Size: 600 1168 50
Fragments arranged by size:
1168 600 50
XhoI C'TCGA_G
Cuts at: 0 1544 1818
Size: 1544 274
XmaI C'CCGG_G
Cuts at: 0 1653 1779 1818
Size: 1653 126 39
Fragments arranged by size:
1653 126 39
XmnI GAAnn'nnTTC
Cuts at: 0 1343 1818
Size: 1343 475
Enzymes that do cut:
AcuI AfeI AleI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BcgI BfrBI BglI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI
SanDI SapI SexAI SfcI SfiI SmaI SmlI SphI
StuI StyI TaqII Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BciVI BclI BglII BlpI BmtI Bpu10I BsaBI
BsaHI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssHII
BstBI BstZ17I ClaI CspCI DraI DraIII EciI EcoRV
FspAI KasI KpnI MfeI MluI MmeI MscI NarI
NdeI NheI NotI NruI PacI PciI PmeI PshAI
PsiI PsrI PvuI RsrII SacII SalI SbfI ScaI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI TstI XbaI ZraI