Restriction map for
pFN21AE9278
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# NO CUT ENZYMES ( 198 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MluNI MscI
MseI MslI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 129 CGCG CG^CG
AccIII 29 TCCGGA T^CCGGA
AlwNI 11 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 147 GGACC G^G[AT]CC
BamHI 19 GGATCC G^GATCC
BbsI 124 GAAGAC GAAGAC(2/6)
BpmI 154 CTGGAG CTGGAG(16/14)
BpuAI 124 GAAGAC GAAGAC(2/6)
BsaWI 29 TCCGGA [AT]^CCGG[AT]
BseAI 29 TCCGGA T^CCGGA
BsiYI 23 CCGGCTTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 23 CCGGCTTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI 29 TCCGGA T^CCGGA
BspMI 59 ACCTGC ACCTGC(4/8)
BstF5I 72 GGATG GGATG(2/0)
BstUI 129 CGCG CG^CG
BstYI 19 GGATCC [GA]^GATC[CT]
CfoI 130 GCGC GCG^C
DpnII 20 GATC ^GATC
DsaI 99 CCGTGG C^C[GA][CT]GG
FokI 72 GGATG GGATG(9/13)
HaeIII 114 GGCC GG^CC
HgaI 127 GACGC GACGC(5/10)
HhaI 130 GCGC GCG^C
HinP1I 130 GCGC G^CGC
HphI 42 GGTGA GGTGA(8/7)
MaeII 143 ACGT A^CGT
MboI 20 GATC ^GATC
MboII 124 GAAGA GAAGA(8/7)
MflI 19 GGATCC [GA]^GATC[CT]
MnlI 4 GAGG CCTC(7/6)
MroI 29 TCCGGA T^CCGGA
MvnI 129 CGCG CG^CG
NdeII 20 GATC ^GATC
NlaIV 19 GGATCC GG[ACGT]^[ACGT]CC
Sau3AI 20 GATC ^GATC
XhoII 19 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 20 GATCC GGATC(4/5)
19 GGATC
BsaJI 99 CCGTGG C^C[ACGT][ACGT]GG
116 CCCAGG
BsgI 55 CTGCAC GTGCAG(16/14)
61 CTGCAC
BssKI 117 CCAGG ^CC[ACGT]GG
180 CCTGG
BstNI 117 CCAGG CC^[AT]GG
180 CCTGG
Cfr13I 114 GGCCC G^G[ACGT]CC
147 GGACC
EcoRII 117 CCAGG ^CC[AT]GG
180 CCTGG
HapII 23 CCGG C^CGG
30 CCGG
HpaII 23 CCGG C^CGG
30 CCGG
MspA1I 151 CAGCTG C[AC]G^C[GT]G
172 CAGCTG
MspI 23 CCGG C^CGG
30 CCGG
MvaI 117 CCAGG CC^[AT]GG
180 CCTGG
PvuII 151 CAGCTG CAG^CTG
172 CAGCTG
Sau96I 114 GGCCC G^G[ACGT]CC
147 GGACC
ScrFI 117 CCAGG CC^[ACGT]GG
180 CCTGG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AciI AluI BbvI Cac8I Fnu4HI
ItaI MwoI TseI