(Circular) (Six-base) MAPSORT of: pFN21AE6131.seq Check: 8443 from: 1 to: 4894 pFN21AE6131 4894 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2704 278 Size: 53 83 186 936 1168 2468 Fragments arranged by size: 2468 1168 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2237 1937 Size: 300 4594 Acc65I G'GTAC_C Cuts at: 2215 2215 Size: 4894 AclI AA'CG_TT Cuts at: 1563 3021 3394 1563 Size: 1458 373 3063 Fragments arranged by size: 3063 1458 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2957 4341 4609 1632 Size: 1325 1384 268 1917 Fragments arranged by size: 1917 1384 1325 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4697 AflIII A'CryG_T Cuts at: 1203 3793 4770 1203 Size: 2590 977 1327 Fragments arranged by size: 2590 1327 977 AgeI A'CCGG_T Cuts at: 3725 3725 Size: 4894 AhdI GACnn_n'nnGTC Cuts at: 3623 3623 Size: 4894 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4894 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4894 AlwNI CAG_nnn'CTG Cuts at: 2637 4209 2637 Size: 1572 3322 ApaI G_GGCC'C Cuts at: 2072 2072 Size: 4894 ApaLI G'TGCA_C Cuts at: 2951 4107 2951 Size: 1156 3738 ApoI r'AATT_y Cuts at: 1493 2080 2203 2429 2581 4690 1493 Size: 587 123 226 152 2109 1697 Fragments arranged by size: 2109 1697 587 226 152 123 AseI AT'TA_AT Cuts at: 160 1032 3448 160 Size: 872 2416 1606 Fragments arranged by size: 2416 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4894 AvaI C'yCGr_G Cuts at: 1870 1943 2219 1870 Size: 73 276 4545 Fragments arranged by size: 4545 276 73 BamHI G'GATC_C Cuts at: 2224 2591 2224 Size: 367 4527 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2215 3670 618 Size: 325 217 627 428 1455 1842 Fragments arranged by size: 1842 1455 627 428 325 217 BanII G_rGCy'C Cuts at: 1391 2072 2213 1391 Size: 681 141 4072 Fragments arranged by size: 4072 681 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3923 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3085 3119 1903 Size: 34 1148 34 3678 Fragments arranged by size: 3678 1148 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2786 3996 2786 Size: 1210 3684 BclI T'GATC_A Cuts at: 1548 1548 Size: 4894 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3505 136 Size: 107 122 71 631 605 1833 1525 Fragments arranged by size: 1833 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4889 4889 Size: 4894 BlpI GC'TnA_GC Cuts at: 2311 2311 Size: 4894 Bme1580I G_kGCm'C Cuts at: 1790 2072 2955 4111 1790 Size: 282 883 1156 2573 Fragments arranged by size: 2573 1156 883 282 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4894 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2625 3583 442 Size: 416 53 83 399 24 1208 958 1753 Fragments arranged by size: 1753 1208 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4894 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3554 1239 Size: 2315 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3019 3884 4182 3019 Size: 865 298 3731 Fragments arranged by size: 3731 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3557 882 Size: 617 2058 2219 Fragments arranged by size: 2219 2058 617 BsaAI yAC'GTr Cuts at: 493 1204 2685 493 Size: 711 1481 2702 Fragments arranged by size: 2702 1481 711 BsaBI GATnn'nnATC Cuts at: 4888 4888 Size: 4894 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2701 3083 3718 275 Size: 53 83 186 936 1168 382 635 1451 Fragments arranged by size: 1451 1168 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2022 3327 3725 3999 4146 4557 2022 Size: 1305 398 274 147 411 2359 Fragments arranged by size: 2359 1305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4669 Fragments arranged by size: 4669 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 4002 BseYI C'CCAG_C Cuts at: 4097 4593 4097 Size: 496 4398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4321 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2344 3105 4133 1540 Size: 275 186 343 761 1028 2301 Fragments arranged by size: 2301 1028 761 343 275 186 BsiHKAI G_wGCw'C Cuts at: 2955 3040 4111 2955 Size: 85 1071 3738 Fragments arranged by size: 3738 1071 85 BsmI GAATG_Cn' Cuts at: 2405 2498 2405 Size: 93 4801 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2213 2955 3040 4111 1391 Size: 399 282 141 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 141 85 BspEI T'CCGG_A Cuts at: 2022 4557 2022 Size: 2535 2359 BspHI T'CATG_A Cuts at: 2783 4513 2783 Size: 1730 3164 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2249 844 Size: 1405 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2781 1394 Size: 429 958 3507 Fragments arranged by size: 3507 958 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3389 3563 65 Size: 1183 2141 174 1396 Fragments arranged by size: 2141 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3538 3725 1700 Size: 110 1728 187 2869 Fragments arranged by size: 2869 1728 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4894 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4894 BssSI C'ACGA_G Cuts at: 2954 3966 2954 Size: 1012 3882 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4894 BstBI TT'CG_AA Cuts at: 3698 3698 Size: 4894 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4894 BstYI r'GATC_y Cuts at: 1972 2141 2224 2591 2976 2993 4445 4531 4543 Size: 169 83 367 385 17 1452 86 12 Cuts at: 4543 4889 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 169 86 83 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4342 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4155 Fragments arranged by size: 4155 683 56 BtsI GCAGTG_nn' Cuts at: 2410 3204 3224 2410 Size: 794 20 4080 Fragments arranged by size: 4080 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4894 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2022 2057 529 Size: 35 1458 35 3366 Fragments arranged by size: 3366 1458 35 35 DraI TTT'AAA Cuts at: 2198 2198 Size: 4894 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3901 809 Size: 507 2585 1802 Fragments arranged by size: 2585 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2341 3230 4859 Size: 54 1425 50 275 18 511 889 1629 Cuts at: 4859 8 Size: 43 Fragments arranged by size: 1629 1425 889 511 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2341 1537 Size: 275 529 4090 Fragments arranged by size: 4090 529 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2824 1353 Size: 1471 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3481 3855 4001 387 Size: 1064 138 1892 374 146 1280 Fragments arranged by size: 1892 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2957 3554 4341 4609 1239 Size: 393 1325 597 787 268 1524 Fragments arranged by size: 1524 1325 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2068 2069 2068 Size: 1 4893 EcoRI G'AATT_C Cuts at: 2203 2581 2203 Size: 378 4516 FspI TGC'GCA Cuts at: 3400 3400 Size: 4894 HaeII r_GCGC'y Cuts at: 1678 4041 1678 Size: 2363 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3535 3567 3609 3641 1373 Size: 32 135 32 1963 32 42 32 2626 Fragments arranged by size: 2626 1963 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2238 2484 669 Size: 988 281 300 246 3079 Fragments arranged by size: 3079 988 300 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4894 HpaI GTT'AAC Cuts at: 2484 2484 Size: 4894 KpnI G_GTAC'C Cuts at: 2219 2219 Size: 4894 MfeI C'AATT_G Cuts at: 2493 2493 Size: 4894 MluI A'CGCG_T Cuts at: 4770 4770 Size: 4894 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4008 4192 1305 Size: 2703 184 2007 Fragments arranged by size: 2703 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3072 Fragments arranged by size: 3072 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2852 3211 3370 518 Size: 648 1686 359 159 2042 Fragments arranged by size: 2042 1686 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2310 2987 4135 4380 1606 Size: 704 677 1148 245 2120 Fragments arranged by size: 2120 1148 704 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4342 NdeI CA'TA_TG Cuts at: 387 387 Size: 4894 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4894 NotI GC'GGCC_GC Cuts at: 2341 2341 Size: 4894 NspI r_CATG'y Cuts at: 1132 1229 2252 3797 1132 Size: 97 1023 1545 2229 Fragments arranged by size: 2229 1545 1023 97 PasI CC'CwG_GG Cuts at: 1348 2072 1348 Size: 724 4170 PciI A'CATG_T Cuts at: 3793 3793 Size: 4894 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4894 PmeI GTTT'AAAC Cuts at: 2198 2198 Size: 4894 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2963 2995 4476 4508 2963 Size: 32 1481 32 3349 Fragments arranged by size: 3349 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4894 PsiI TTA'TAA Cuts at: 2464 2464 Size: 4894 PspOMI G'GGCC_C Cuts at: 2068 2068 Size: 4894 PstI C_TGCA'G Cuts at: 830 2246 830 Size: 1416 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4894 SalI G'TCGA_C Cuts at: 1936 2236 1936 Size: 300 4594 SbfI CC_TGCA'GG Cuts at: 2246 2246 Size: 4894 ScaI AGT'ACT Cuts at: 3142 4854 3142 Size: 1712 3182 SfcI C'TryA_G Cuts at: 826 1045 2242 3377 4058 4249 826 Size: 219 1197 1135 681 191 1471 Fragments arranged by size: 1471 1197 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4894 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4894 SmaI CCC'GGG Cuts at: 1872 2221 1872 Size: 349 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2998 3899 4161 820 Size: 197 926 1055 901 262 1553 Fragments arranged by size: 1553 1055 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2685 493 Size: 2192 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4894 SphI G_CATG'C Cuts at: 1132 2252 1132 Size: 1120 3774 SspI AAT'ATT Cuts at: 5 52 2818 5 Size: 47 2766 2081 Fragments arranged by size: 2766 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4894 StyI C'CwwG_G Cuts at: 513 1065 2333 513 Size: 552 1268 3074 Fragments arranged by size: 3074 1268 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2921 2938 3091 3276 3721 325 Size: 1255 1341 17 153 185 445 1498 Fragments arranged by size: 1498 1341 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 3140 4852 Size: 274 80 33 51 1443 156 1007 1712 Cuts at: 4852 96 Size: 138 Fragments arranged by size: 1712 1443 1007 274 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 4854 4886 2090 Size: 32 2732 32 2098 Fragments arranged by size: 2732 2098 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4894 XbaI T'CTAG_A Cuts at: 2230 2230 Size: 4894 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4894 XmaI C'CCGG_G Cuts at: 1870 2219 1870 Size: 349 4545 XmnI GAAnn'nnTTC Cuts at: 3023 3023 Size: 4894 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2702 276 Size: 53 83 186 936 1168 2468 Fragments arranged by size: 2468 1168 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI