################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AclI AflII AgeI Aor51HI AscI Asp718I AspI AvrII BbeI BcgI BclI BfrI BlnI BsaBI BsiWI Bsp1407I BspDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI DraI Eco47III EcoO65I FbaI FseI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshAI Psp1406I PvuI SacII SbfI SexAI SfiI SfoI SfuI SpeI Sse8387I SspBI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 621 GTCGAC GT^[AC][GT]AC AccIII 1776 TCCGGA T^CCGGA AcsI 123 GAATTC [GA]^AATT[CT] AhdI 2125 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1677 CCTCAGG CC^T[ACGT]AGG ApoI 123 GAATTC [GA]^AATT[CT] AseI 1984 ATTAAT AT^TAAT AsnI 1984 ATTAAT AT^TAAT AspEI 2125 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2051 TGCGCA TGC^GCA BamHI 3037 GGATCC G^GATCC BanI 2914 GGCACC G^G[CT][GA]CC BbvCI 2860 GCTGAGG CCTCAGC(-5/-2) BciVI 3267 GGATAC GTATCC(6/5) BlpI 3157 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3157 GCTAAGC GC^T[ACGT]AGC BseAI 1776 TCCGGA T^CCGGA BsmBI 3397 GAGACG CGTCTC(1/5) BsmI 2852 GCATTC GAATGC(1/-1) BspEI 1776 TCCGGA T^CCGGA BspLU11I 651 ACATGT A^CATGT BstXI 509 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1677 CCTCAGG CC^T[ACGT]AGG CelII 3157 GCTAAGC GC^T[ACGT]AGC CpoI 1727 CGGTCCG CG^G[AT]CCG DrdI 2407 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2125 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1677 CCTCAGG CC^T[ACGT]AGG EcoNI 2651 CCTTTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 123 GAATTC G^AATTC EcoRV 429 GATATC GAT^ATC EcoT22I 251 ATGCAT ATGCA^T FspI 2051 TGCGCA TGC^GCA MroI 1776 TCCGGA T^CCGGA MseI 1985 TTAA T^TAA NsiI 251 ATGCAT ATGCA^T PaeR7I 2786 CTCGAG C^TCGAG PpuMI 3654 AGGTCCC [GA]G^G[AT]CC[CT] PshBI 1984 ATTAAT AT^TAAT RsrII 1727 CGGTCCG CG^G[AT]CCG SalI 621 GTCGAC G^TCGAC SapI 1515 GAAGAGC GCTCTTC(1/4) ScaI 2546 AGTACT AGT^ACT SgrAI 2146 CACCGGCG C[GA]^CCGG[CT]G SmaI 3033 CCCGGG CCC^GGG SnaBI 86 TACGTA TAC^GTA SspI 2752 AATATT AAT^ATT StuI 2096 AGGCCT AGG^CCT Tru9I 1985 TTAA T^TAA Tsp509I 124 AATT ^AATT XhoI 2786 CTCGAG C^TCGAG XmaCI 3033 CCCGGG C^CCGGG XmaI 3033 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AatII 580 GACGTC GACGT^C 3399 GACGTC AccII 976 CGCG CG^CG 1732 CGCG AflIII 651 ACATGT A^C[GA][CT]GT 1449 ACACGT ApaI 1201 GGGCCC GGGCC^C 1863 GGGCCC BalI 2081 TGGCCA TGG^CCA 3012 TGGCCA BbsI 577 GAAGAC GAAGAC(2/6) 2346 GAAGAC BglI 1645 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3335 GCCACGATGGC BglII 3419 AGATCT A^GATCT 3584 AGATCT BpuAI 577 GAAGAC GAAGAC(2/6) 2346 GAAGAC BsaWI 1587 ACCGGA [AT]^CCGG[AT] 1776 TCCGGA BspHI 2495 TCATGA T^CATGA 2948 TCATGA BsrBI 1708 CCGCTC CCGCTC(-3/-3) 1936 CCGCTC BstUI 976 CGCG CG^CG 1732 CGCG DraIII 2284 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG 3099 CACCCAGTG HaeII 1555 AGCGCC [GA]GCGC^[CT] 1856 GGCGCT MluNI 2081 TGGCCA TGG^CCA 3012 TGGCCA MscI 2081 TGGCCA TGG^CCA 3012 TGGCCA MvnI 976 CGCG CG^CG 1732 CGCG NaeI 1853 GCCGGC GCC^GGC 2354 GCCGGC NgoMIV 1853 GCCGGC G^CCGGC 2354 GCCGGC RcaI 2495 TCATGA T^CATGA 2948 TCATGA SphI 141 GCATGC GCATG^C 2000 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI Asp700I AspHI AvaI AvaII BanII BbrPI BbvI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmnI