(Circular) (Six-base) MAPSORT of: pFN21AE0899.seq Check: 5362 from: 1 to: 8443 pFN21AE0899 8443 bp With 182 enzymes: SgfI * November 8, 2010 14:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2945 2945 Size: 8443 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2589 5408 6253 278 Size: 53 83 186 936 1053 2819 845 2468 Fragments arranged by size: 2819 2468 1053 936 845 186 83 53 AccI GT'mk_AC Cuts at: 1937 2627 5786 1937 Size: 690 3159 4594 Fragments arranged by size: 4594 3159 690 Acc65I G'GTAC_C Cuts at: 5764 5764 Size: 8443 AclI AA'CG_TT Cuts at: 1563 6570 6943 1563 Size: 5007 373 3063 Fragments arranged by size: 5007 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2280 2784 5558 6506 7890 8158 1632 Size: 503 145 504 2774 948 1384 268 1917 Fragments arranged by size: 2774 1917 1384 948 504 503 268 145 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8246 AflIII A'CryG_T Cuts at: 1203 2656 3454 7342 8319 1203 Size: 1453 798 3888 977 1327 Fragments arranged by size: 3888 1453 1327 977 798 AgeI A'CCGG_T Cuts at: 7274 7274 Size: 8443 AhdI GACnn_n'nnGTC Cuts at: 4135 7172 4135 Size: 3037 5406 AleI CACnn'nnGTG Cuts at: 1166 4626 4722 4833 1166 Size: 3460 96 111 4776 Fragments arranged by size: 4776 3460 111 96 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3188 4612 1184 Size: 2004 1424 5015 Fragments arranged by size: 5015 2004 1424 AlwNI CAG_nnn'CTG Cuts at: 3886 4939 5437 6186 7758 3886 Size: 1053 498 749 1572 4571 Fragments arranged by size: 4571 1572 1053 749 498 ApaI G_GGCC'C Cuts at: 3210 3872 3210 Size: 662 7781 ApaLI G'TGCA_C Cuts at: 3602 4592 4994 5090 6500 7656 3602 Size: 990 402 96 1410 1156 4389 Fragments arranged by size: 4389 1410 1156 990 402 96 ApoI r'AATT_y Cuts at: 1493 2128 5752 5978 6130 8239 1493 Size: 635 3624 226 152 2109 1697 Fragments arranged by size: 3624 2109 1697 635 226 152 AseI AT'TA_AT Cuts at: 160 1032 3990 6997 160 Size: 872 2958 3007 1606 Fragments arranged by size: 3007 2958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8443 AvaI C'yCGr_G Cuts at: 1870 1943 3182 3619 3794 4791 5038 5768 1870 Size: 73 1239 437 175 997 247 730 4545 Fragments arranged by size: 4545 1239 997 730 437 247 175 73 BamHI G'GATC_C Cuts at: 5042 5773 6140 5042 Size: 731 367 7345 Fragments arranged by size: 7345 731 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4919 5764 7219 618 Size: 325 217 627 3132 845 1455 1842 Fragments arranged by size: 3132 1842 1455 845 627 325 217 BanII G_rGCy'C Cuts at: 1391 2190 3210 3872 3939 3963 4272 4284 5268 Size: 799 1020 662 67 24 309 12 984 Cuts at: 5268 5738 5762 1391 Size: 470 24 4072 Fragments arranged by size: 4072 1020 984 799 662 470 309 67 24 24 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2589 4358 928 Size: 971 690 1769 5013 Fragments arranged by size: 5013 1769 971 690 BbvCI CC'TCA_GC Cuts at: 4866 4866 Size: 8443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6634 6668 1903 Size: 34 4697 34 3678 Fragments arranged by size: 4697 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5266 6335 7545 5266 Size: 1069 1210 6164 Fragments arranged by size: 6164 1210 1069 BclI T'GATC_A Cuts at: 1548 1548 Size: 8443 BfrBI ATG'CAT Cuts at: 2258 2258 Size: 8443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3656 5346 7054 Size: 107 122 71 631 605 1984 1690 1708 Cuts at: 7054 136 Size: 1525 Fragments arranged by size: 1984 1708 1690 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5424 5589 8438 5424 Size: 165 2849 5429 Fragments arranged by size: 5429 2849 165 BlpI GC'TnA_GC Cuts at: 5163 5860 5163 Size: 697 7746 Bme1580I G_kGCm'C Cuts at: 1790 3210 3606 3872 4596 4998 5094 5691 6504 Size: 1420 396 266 724 402 96 597 813 Cuts at: 6504 7660 1790 Size: 1156 2573 Fragments arranged by size: 2573 1420 1156 813 724 597 402 396 266 96 BmgBI CAC'GTC Cuts at: 1318 5686 1318 Size: 4368 4075 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3099 3213 4339 Size: 416 53 83 399 24 1682 114 1126 Cuts at: 4339 4631 5101 6174 7132 442 Size: 292 470 1073 958 1753 Fragments arranged by size: 1753 1682 1126 1073 958 470 416 399 292 114 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2373 2406 3506 4181 4214 4576 4755 5510 Size: 1134 33 1100 675 33 362 179 755 Cuts at: 5510 5695 7103 1239 Size: 185 1408 2579 Fragments arranged by size: 2579 1408 1134 1100 755 675 362 185 179 33 33 Bpu10I CC'TnA_GC Cuts at: 2892 3741 4377 4866 5267 2892 Size: 849 636 489 401 6068 Fragments arranged by size: 6068 849 636 489 401 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3209 4310 6568 7433 7731 3209 Size: 1101 2258 865 298 3921 Fragments arranged by size: 3921 2258 1101 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4788 4794 5123 7106 882 Size: 617 3289 6 329 1983 2219 Fragments arranged by size: 3289 2219 1983 617 329 6 BsaAI yAC'GTr Cuts at: 493 1204 2093 3457 4585 5509 6234 493 Size: 711 889 1364 1128 924 725 2702 Fragments arranged by size: 2702 1364 1128 924 889 725 711 BsaBI GATnn'nnATC Cuts at: 8437 8437 Size: 8443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2586 3191 4419 5405 Size: 53 83 186 936 1053 605 1228 986 Cuts at: 5405 6250 6632 7267 275 Size: 845 382 635 1451 Fragments arranged by size: 1451 1228 1053 986 936 845 635 605 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3592 3781 6876 7274 7548 7695 8106 3592 Size: 189 3095 398 274 147 411 3929 Fragments arranged by size: 3929 3095 411 398 274 189 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3548 3578 4106 4136 4958 Size: 30 165 30 2145 30 528 30 822 Cuts at: 4958 4988 1178 Size: 30 4633 Fragments arranged by size: 4633 2145 822 528 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 2576 3343 4160 4418 4421 4883 4886 Size: 1378 12 767 817 258 3 462 3 Cuts at: 4886 5311 5428 1186 Size: 425 117 4201 Fragments arranged by size: 4201 1378 817 767 462 425 258 117 12 3 3 BseYI C'CCAG_C Cuts at: 2162 2378 3853 3864 4531 7646 8142 2162 Size: 216 1475 11 667 3115 496 2463 Fragments arranged by size: 3115 2463 1475 667 496 216 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2973 3784 3899 5027 1163 Size: 573 1237 811 115 1128 4579 Fragments arranged by size: 4579 1237 1128 811 573 115 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2888 3432 3500 3516 5893 6654 Size: 275 186 887 544 68 16 2377 761 Cuts at: 6654 7682 1540 Size: 1028 2301 Fragments arranged by size: 2377 2301 1028 887 761 544 275 186 68 16 BsiHKAI G_wGCw'C Cuts at: 3527 3606 3939 3963 4152 4596 4998 5094 5268 Size: 79 333 24 189 444 402 96 174 Cuts at: 5268 5500 5738 6504 6589 7660 3527 Size: 232 238 766 85 1071 4310 Fragments arranged by size: 4310 1071 766 444 402 333 238 232 189 174 96 85 79 24 BsmI GAATG_Cn' Cuts at: 4857 5954 6047 4857 Size: 1097 93 7253 Fragments arranged by size: 7253 1097 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5396 5396 Size: 8443 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 3210 3527 3606 3872 3939 3963 Size: 399 400 1020 317 79 266 67 24 Cuts at: 3963 4152 4272 4284 4596 4998 5094 5268 5500 Size: 189 120 12 312 402 96 174 232 Cuts at: 5500 5691 5738 5762 6504 6589 7660 1391 Size: 191 47 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 1020 742 402 400 399 317 312 266 232 191 189 174 120 96 85 79 67 47 24 24 12 BspEI T'CCGG_A Cuts at: 3781 8106 3781 Size: 4325 4118 BspHI T'CATG_A Cuts at: 4500 4953 6332 8062 4500 Size: 453 1379 1730 4881 Fragments arranged by size: 4881 1730 1379 453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2682 2945 3228 5798 844 Size: 1838 263 283 2570 3489 Fragments arranged by size: 3489 2570 1838 283 263 BsrBI CCG'CTC Cuts at: 1394 1823 3715 3943 6330 1394 Size: 429 1892 228 2387 3507 Fragments arranged by size: 3507 2387 1892 429 228 BsrDI GCAATG_nn' Cuts at: 65 1248 2157 2408 3157 6938 7112 65 Size: 1183 909 251 749 3781 174 1396 Fragments arranged by size: 3781 1396 1183 909 749 251 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3858 4080 4152 4359 7087 7274 1700 Size: 110 2048 222 72 207 2728 187 2869 Fragments arranged by size: 2869 2728 2048 222 207 187 110 72 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8443 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8443 BssSI C'ACGA_G Cuts at: 6503 7515 6503 Size: 1012 7431 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8443 BstBI TT'CG_AA Cuts at: 7247 7247 Size: 8443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8443 BstXI CCAn_nnnn'nTGG Cuts at: 2521 2521 Size: 8443 BstYI r'GATC_y Cuts at: 1972 3672 4188 5042 5424 5589 5773 6140 6525 Size: 1700 516 854 382 165 184 367 385 Cuts at: 6525 6542 7994 8080 8092 8438 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1700 1452 854 516 385 382 367 346 184 165 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3683 3683 Size: 8443 BtgI C'CryG_G Cuts at: 513 1065 2069 2829 3024 3510 3663 3927 4288 Size: 552 1004 760 195 486 153 264 361 Cuts at: 4288 5175 5451 513 Size: 887 276 3505 Fragments arranged by size: 3505 1004 887 760 552 486 361 276 264 195 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7704 Fragments arranged by size: 7704 683 56 BtsI GCAGTG_nn' Cuts at: 3018 4594 4596 4633 5959 6753 6773 3018 Size: 1576 2 37 1326 794 20 4688 Fragments arranged by size: 4688 1576 1326 794 37 20 2 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3214 3249 529 Size: 35 2650 35 5723 Fragments arranged by size: 5723 2650 35 35 DraI TTT'AAA Cuts at: 5747 5747 Size: 8443 DraIII CAC_nnn'GTG Cuts at: 4294 5109 4294 Size: 815 7628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4418 7450 809 Size: 507 3102 3032 1802 Fragments arranged by size: 3102 3032 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2841 3429 3497 Size: 54 1425 50 275 18 1011 588 68 Cuts at: 3497 3513 3660 4086 4357 5017 5890 6779 8408 Size: 16 147 426 271 660 873 889 1629 Cuts at: 8408 8 Size: 43 Fragments arranged by size: 1629 1425 1011 889 873 660 588 426 275 271 147 68 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3429 3497 3513 5890 1537 Size: 275 1617 68 16 2377 4090 Fragments arranged by size: 4090 2377 1617 275 68 16 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 3333 3515 4376 4395 4563 6373 1353 Size: 779 1201 182 861 19 168 1810 3423 Fragments arranged by size: 3423 1810 1201 861 779 182 168 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3368 3569 3682 5378 7030 7404 Size: 1064 138 1779 201 113 1696 1652 374 Cuts at: 7404 7550 387 Size: 146 1280 Fragments arranged by size: 1779 1696 1652 1280 1064 374 201 146 138 113 EcoICRI GAG'CTC Cuts at: 3937 3961 5266 5736 3937 Size: 24 1305 470 6644 Fragments arranged by size: 6644 1305 470 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2280 2373 2406 2784 3506 4181 Size: 393 503 145 93 33 378 722 675 Cuts at: 4181 4214 4576 4755 5510 5558 5695 6506 7103 Size: 33 362 179 755 48 137 811 597 Cuts at: 7103 7890 8158 1239 Size: 787 268 1524 Fragments arranged by size: 1524 811 787 755 722 675 597 503 393 378 362 268 179 145 137 93 48 33 33 EcoNI CCTnn'n_nnAGG Cuts at: 4660 4660 Size: 8443 EcoO109I rG'GnC_Cy Cuts at: 3292 3410 3424 3687 3868 3869 5660 3292 Size: 118 14 263 181 1 1791 6075 Fragments arranged by size: 6075 1791 263 181 118 14 1 EcoRI G'AATT_C Cuts at: 2128 5752 6130 2128 Size: 3624 378 4441 Fragments arranged by size: 4441 3624 378 EcoRV GAT'ATC Cuts at: 2436 2436 Size: 8443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2492 2492 Size: 8443 FspI TGC'GCA Cuts at: 4058 6949 4058 Size: 2891 5552 HaeII r_GCGC'y Cuts at: 1678 3564 3865 7590 1678 Size: 1886 301 3725 2531 Fragments arranged by size: 3725 2531 1886 301 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 3057 3089 3548 Size: 32 135 32 671 32 782 32 459 Cuts at: 3548 3580 3763 3795 4121 4153 4662 4694 5404 Size: 32 183 32 326 32 509 32 710 Cuts at: 5404 5436 5560 5592 7084 7116 7158 7190 1373 Size: 32 124 32 1492 32 42 32 2626 Fragments arranged by size: 2626 1492 782 710 671 509 459 326 183 135 124 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2628 2697 3118 5787 6033 669 Size: 988 281 690 69 421 2669 246 3079 Fragments arranged by size: 3079 2669 988 690 421 281 246 69 HindIII A'AGCT_T Cuts at: 748 748 Size: 8443 HpaI GTT'AAC Cuts at: 6033 6033 Size: 8443 KpnI G_GTAC'C Cuts at: 5768 5768 Size: 8443 MfeI C'AATT_G Cuts at: 6042 6042 Size: 8443 MluI A'CGCG_T Cuts at: 8319 8319 Size: 8443 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5379 7557 7741 1305 Size: 4074 2178 184 2007 Fragments arranged by size: 4074 2178 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4088 5019 10 Size: 54 1425 343 2256 931 3434 Fragments arranged by size: 3434 2256 1425 931 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2617 2655 2936 3926 4505 4626 4722 Size: 648 1451 38 281 990 579 121 96 Cuts at: 4722 4833 6401 6760 6919 518 Size: 111 1568 359 159 2042 Fragments arranged by size: 2042 1568 1451 990 648 579 359 281 159 121 111 96 38 MspA1I CmG'CkG Cuts at: 1606 2819 3113 3283 3301 3883 4213 5299 5859 Size: 1213 294 170 18 582 330 1086 560 Cuts at: 5859 6536 7684 7929 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1213 1148 1086 677 582 560 330 294 245 170 18 NaeI GCC'GGC Cuts at: 3860 4361 3860 Size: 501 7942 NcoI C'CATG_G Cuts at: 513 1065 3663 3927 5175 5451 513 Size: 552 2598 264 1248 276 3505 Fragments arranged by size: 3505 2598 1248 552 276 264 NdeI CA'TA_TG Cuts at: 387 387 Size: 8443 NgoMIV G'CCGG_C Cuts at: 3858 4359 3858 Size: 501 7942 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8443 NotI GC'GGCC_GC Cuts at: 5890 5890 Size: 8443 NsiI A_TGCA'T Cuts at: 2260 2260 Size: 8443 NspI r_CATG'y Cuts at: 1132 1229 2150 2660 2675 4009 4054 4510 5801 Size: 97 921 510 15 1334 45 456 1291 Cuts at: 5801 7346 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1334 1291 921 510 456 97 45 15 PasI CC'CwG_GG Cuts at: 1348 4925 1348 Size: 3577 4866 PciI A'CATG_T Cuts at: 2656 7342 2656 Size: 4686 3757 PflMI CCAn_nnn'nTGG Cuts at: 1108 3853 4294 5109 1108 Size: 2745 441 815 4442 Fragments arranged by size: 4442 2745 815 441 PfoI T'CCnGG_A Cuts at: 4191 5256 4191 Size: 1065 7378 PmeI GTTT'AAAC Cuts at: 5747 5747 Size: 8443 PmlI CAC'GTG Cuts at: 3457 4585 5509 3457 Size: 1128 924 6391 Fragments arranged by size: 6391 1128 924 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6512 6544 8025 8057 6512 Size: 32 1481 32 6898 Fragments arranged by size: 6898 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5660 5660 Size: 8443 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8443 PsiI TTA'TAA Cuts at: 6013 6013 Size: 8443 PspOMI G'GGCC_C Cuts at: 3206 3868 3206 Size: 662 7781 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2073 2105 2073 Size: 32 8411 PstI C_TGCA'G Cuts at: 830 3300 4098 4935 5397 5795 830 Size: 2470 798 837 462 398 3478 Fragments arranged by size: 3478 2470 837 798 462 398 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8443 PvuII CAG'CTG Cuts at: 3283 3301 5299 3283 Size: 18 1998 6427 Fragments arranged by size: 6427 1998 18 RsrII CG'GwC_CG Cuts at: 3733 3733 Size: 8443 SacI G_AGCT'C Cuts at: 3939 3963 5268 5738 3939 Size: 24 1305 470 6644 Fragments arranged by size: 6644 1305 470 24 SalI G'TCGA_C Cuts at: 1936 2626 5785 1936 Size: 690 3159 4594 Fragments arranged by size: 4594 3159 690 SapI GCTCTTCn'nnn_ Cuts at: 3515 3515 Size: 8443 SbfI CC_TGCA'GG Cuts at: 5795 5795 Size: 8443 ScaI AGT'ACT Cuts at: 4553 6691 8403 4553 Size: 2138 1712 4593 Fragments arranged by size: 4593 2138 1712 SfcI C'TryA_G Cuts at: 826 1045 3296 4094 4931 5393 5791 6926 7607 Size: 219 2251 798 837 462 398 1135 681 Cuts at: 7607 7798 826 Size: 191 1471 Fragments arranged by size: 2251 1471 1135 837 798 681 462 398 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8443 SgrAI Cr'CCGG_yG Cuts at: 1700 4152 1700 Size: 2452 5991 SmaI CCC'GGG Cuts at: 1872 5040 5770 1872 Size: 3168 730 4545 Fragments arranged by size: 4545 3168 730 SmlI C'TyrA_G Cuts at: 820 1017 1943 3224 4325 4791 6547 7448 7710 Size: 197 926 1281 1101 466 1756 901 262 Cuts at: 7710 820 Size: 1553 Fragments arranged by size: 1756 1553 1281 1101 926 901 466 262 197 SnaBI TAC'GTA Cuts at: 493 2093 6234 493 Size: 1600 4141 2702 Fragments arranged by size: 4141 2702 1600 SpeI A'CTAG_T Cuts at: 152 152 Size: 8443 SphI G_CATG'C Cuts at: 1132 2150 4009 5801 1132 Size: 1018 1859 1792 3774 Fragments arranged by size: 3774 1859 1792 1018 SspI AAT'ATT Cuts at: 5 52 4759 6367 5 Size: 47 4707 1608 2081 Fragments arranged by size: 4707 2081 1608 47 StuI AGG'CCT Cuts at: 1517 4103 1517 Size: 2586 5857 StyI C'CwwG_G Cuts at: 513 1065 2191 2844 3663 3696 3927 5136 5175 Size: 552 1126 653 819 33 231 1209 39 Cuts at: 5175 5451 5882 513 Size: 276 431 3074 Fragments arranged by size: 3074 1209 1126 819 653 552 431 276 231 39 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2874 2876 2939 3721 5120 6470 6487 Size: 1255 1294 2 63 782 1399 1350 17 Cuts at: 6487 6640 6825 7270 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1399 1350 1294 1255 782 445 185 153 63 17 2 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3015 4551 6689 Size: 274 80 33 51 1443 1038 1536 2138 Cuts at: 6689 8401 96 Size: 1712 138 Fragments arranged by size: 2138 1712 1536 1443 1038 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4958 4990 5034 5066 8403 8435 4958 Size: 32 44 32 3337 32 4966 Fragments arranged by size: 4966 3337 44 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8443 XbaI T'CTAG_A Cuts at: 5779 5779 Size: 8443 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3331 4352 3067 Size: 264 1021 7158 Fragments arranged by size: 7158 1021 264 XhoI C'TCGA_G Cuts at: 1943 4791 1943 Size: 2848 5595 XmaI C'CCGG_G Cuts at: 1870 5038 5768 1870 Size: 3168 730 4545 Fragments arranged by size: 4545 3168 730 XmnI GAAnn'nnTTC Cuts at: 2120 2472 2715 4310 6572 2120 Size: 352 243 1595 2262 3991 Fragments arranged by size: 3991 2262 1595 352 243 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2587 5406 6251 276 Size: 53 83 186 936 1053 2819 845 2468 Fragments arranged by size: 2819 2468 1053 936 845 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I FseI FspAI KasI NarI NruI PacI PspXI SacII SanDI SexAI SfiI SfoI SrfI SwaI