(Linear) (Six-base) MAPSORT of: KIEE0899.seq Check: 2169 from: 1 to: 3738 KIEE0899.orf With 182 enzymes: SgfI * November 8, 2010 13:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 940 3738 Size: 940 2798 AatII G_ACGT'C Cuts at: 0 584 3403 3738 Size: 584 2819 335 Fragments arranged by size: 2819 584 335 AccI GT'mk_AC Cuts at: 0 622 3738 Size: 622 3116 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 275 779 3553 3738 Size: 130 145 504 2774 185 Fragments arranged by size: 2774 504 185 145 130 AflIII A'CryG_T Cuts at: 0 651 1449 3738 Size: 651 798 2289 Fragments arranged by size: 2289 798 651 AhdI GACnn_n'nnGTC Cuts at: 0 2130 3738 Size: 2130 1608 AleI CACnn'nnGTG Cuts at: 0 2621 2717 2828 3738 Size: 2621 96 111 910 Fragments arranged by size: 2621 910 111 96 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1183 2607 3738 Size: 1183 1424 1131 Fragments arranged by size: 1424 1183 1131 AlwNI CAG_nnn'CTG Cuts at: 0 1881 2934 3432 3738 Size: 1881 1053 498 306 Fragments arranged by size: 1881 1053 498 306 ApaI G_GGCC'C Cuts at: 0 1205 1867 3738 Size: 1205 662 1871 Fragments arranged by size: 1871 1205 662 ApaLI G'TGCA_C Cuts at: 0 1597 2587 2989 3085 3738 Size: 1597 990 402 96 653 Fragments arranged by size: 1597 990 653 402 96 ApoI r'AATT_y Cuts at: 0 123 3738 Size: 123 3615 AseI AT'TA_AT Cuts at: 0 1985 3738 Size: 1985 1753 AvaI C'yCGr_G Cuts at: 0 1177 1614 1789 2786 3033 3738 Size: 1177 437 175 997 247 705 Fragments arranged by size: 1177 997 705 437 247 175 BamHI G'GATC_C Cuts at: 0 3037 3738 Size: 3037 701 BanI G'GyrC_C Cuts at: 0 2914 3738 Size: 2914 824 BanII G_rGCy'C Cuts at: 0 185 1205 1867 1934 1958 2267 2279 3263 Size: 185 1020 662 67 24 309 12 984 Cuts at: 3263 3733 3738 Size: 470 5 Fragments arranged by size: 1020 984 662 470 309 185 67 24 12 5 BbsI GAAGACnn'nnnn_ Cuts at: 0 584 2353 3738 Size: 584 1769 1385 Fragments arranged by size: 1769 1385 584 BbvCI CC'TCA_GC Cuts at: 0 2861 3738 Size: 2861 877 BciVI GTATCCnnnnn_n' Cuts at: 0 3261 3738 Size: 3261 477 BfrBI ATG'CAT Cuts at: 0 253 3738 Size: 253 3485 BglI GCCn_nnn'nGGC Cuts at: 0 1651 3341 3738 Size: 1651 1690 397 Fragments arranged by size: 1690 1651 397 BglII A'GATC_T Cuts at: 0 3419 3584 3738 Size: 3419 165 154 Fragments arranged by size: 3419 165 154 BlpI GC'TnA_GC Cuts at: 0 3158 3738 Size: 3158 580 Bme1580I G_kGCm'C Cuts at: 0 1205 1601 1867 2591 2993 3089 3686 3738 Size: 1205 396 266 724 402 96 597 52 Fragments arranged by size: 1205 724 597 402 396 266 96 52 BmgBI CAC'GTC Cuts at: 0 3681 3738 Size: 3681 57 BmrI ACTGGGnnnn_n' Cuts at: 0 1094 1208 2334 2626 3096 3738 Size: 1094 114 1126 292 470 642 Fragments arranged by size: 1126 1094 642 470 292 114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 368 401 1501 2176 2209 2571 2750 3505 Size: 368 33 1100 675 33 362 179 755 Cuts at: 3505 3690 3738 Size: 185 48 Fragments arranged by size: 1100 755 675 368 362 185 179 48 33 33 Bpu10I CC'TnA_GC Cuts at: 0 887 1736 2372 2861 3262 3738 Size: 887 849 636 489 401 476 Fragments arranged by size: 887 849 636 489 476 401 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1204 2305 3738 Size: 1204 1101 1433 Fragments arranged by size: 1433 1204 1101 BsaI GGTCTCn'nnnn_ Cuts at: 0 2783 2789 3118 3738 Size: 2783 6 329 620 Fragments arranged by size: 2783 620 329 6 BsaAI yAC'GTr Cuts at: 0 88 1452 2580 3504 3738 Size: 88 1364 1128 924 234 Fragments arranged by size: 1364 1128 924 234 88 BsaHI Gr'CG_yC Cuts at: 0 581 1186 2414 3400 3738 Size: 581 605 1228 986 338 Fragments arranged by size: 1228 986 605 581 338 BsaWI w'CCGG_w Cuts at: 0 1587 1776 3738 Size: 1587 189 1962 Fragments arranged by size: 1962 1587 189 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1543 1573 2101 2131 2953 2983 3738 Size: 1543 30 528 30 822 30 755 Fragments arranged by size: 1543 822 755 528 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 559 571 1338 2155 2413 2416 2878 2881 Size: 559 12 767 817 258 3 462 3 Cuts at: 2881 3306 3423 3738 Size: 425 117 315 Fragments arranged by size: 817 767 559 462 425 315 258 117 12 3 3 BseYI C'CCAG_C Cuts at: 0 157 373 1848 1859 2526 3738 Size: 157 216 1475 11 667 1212 Fragments arranged by size: 1475 1212 667 216 157 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 968 1779 1894 3022 3738 Size: 968 811 115 1128 716 Fragments arranged by size: 1128 968 811 716 115 BsiEI CG_ry'CG Cuts at: 0 883 1427 1495 1511 3738 Size: 883 544 68 16 2227 Fragments arranged by size: 2227 883 544 68 16 BsiHKAI G_wGCw'C Cuts at: 0 1522 1601 1934 1958 2147 2591 2993 3089 Size: 1522 79 333 24 189 444 402 96 Cuts at: 3089 3263 3495 3733 3738 Size: 174 232 238 5 Fragments arranged by size: 1522 444 402 333 238 232 189 174 96 79 24 5 BsmI GAATG_Cn' Cuts at: 0 2852 3738 Size: 2852 886 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3391 3738 Size: 3391 347 Bsp1286I G_dGCh'C Cuts at: 0 185 1205 1522 1601 1867 1934 1958 2147 Size: 185 1020 317 79 266 67 24 189 Cuts at: 2147 2267 2279 2591 2993 3089 3263 3495 3686 Size: 120 12 312 402 96 174 232 191 Cuts at: 3686 3733 3738 Size: 47 5 Fragments arranged by size: 1020 402 317 312 266 232 191 189 185 174 120 96 79 67 47 24 12 5 BspEI T'CCGG_A Cuts at: 0 1776 3738 Size: 1776 1962 BspHI T'CATG_A Cuts at: 0 2495 2948 3738 Size: 2495 453 790 Fragments arranged by size: 2495 790 453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 677 940 1223 3738 Size: 677 263 283 2515 Fragments arranged by size: 2515 677 283 263 BsrBI CCG'CTC Cuts at: 0 1710 1938 3738 Size: 1710 228 1800 Fragments arranged by size: 1800 1710 228 BsrDI GCAATG_nn' Cuts at: 0 152 403 1152 3738 Size: 152 251 749 2586 Fragments arranged by size: 2586 749 251 152 BsrFI r'CCGG_y Cuts at: 0 1853 2075 2147 2354 3738 Size: 1853 222 72 207 1384 Fragments arranged by size: 1853 1384 222 207 72 BstXI CCAn_nnnn'nTGG Cuts at: 0 516 3738 Size: 516 3222 BstYI r'GATC_y Cuts at: 0 1667 2183 3037 3419 3584 3738 Size: 1667 516 854 382 165 154 Fragments arranged by size: 1667 854 516 382 165 154 Bsu36I CC'TnA_GG Cuts at: 0 1678 3738 Size: 1678 2060 BtgI C'CryG_G Cuts at: 0 64 824 1019 1505 1658 1922 2283 3170 Size: 64 760 195 486 153 264 361 887 Cuts at: 3170 3446 3738 Size: 276 292 Fragments arranged by size: 887 760 486 361 292 276 264 195 153 64 BtsI GCAGTG_nn' Cuts at: 0 1013 2589 2591 2628 3738 Size: 1013 1576 2 37 1110 Fragments arranged by size: 1576 1110 1013 37 2 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1209 1244 3738 Size: 1209 35 2494 Fragments arranged by size: 2494 1209 35 DraIII CAC_nnn'GTG Cuts at: 0 2289 3104 3738 Size: 2289 815 634 Fragments arranged by size: 2289 815 634 DrdI GACnn_nn'nnGTC Cuts at: 0 2413 3738 Size: 2413 1325 EaeI y'GGCC_r Cuts at: 0 836 1424 1492 1508 1655 2081 2352 3012 Size: 836 588 68 16 147 426 271 660 Cuts at: 3012 3738 Size: 726 Fragments arranged by size: 836 726 660 588 426 271 147 68 16 EagI C'GGCC_G Cuts at: 0 1424 1492 1508 3738 Size: 1424 68 16 2230 Fragments arranged by size: 2230 1424 68 16 EarI CTCTTCn'nnn_ Cuts at: 0 127 1328 1510 2371 2390 2558 3738 Size: 127 1201 182 861 19 168 1180 Fragments arranged by size: 1201 1180 861 182 168 127 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1363 1564 1677 3373 3738 Size: 1363 201 113 1696 365 Fragments arranged by size: 1696 1363 365 201 113 EcoICRI GAG'CTC Cuts at: 0 1932 1956 3261 3731 3738 Size: 1932 24 1305 470 7 Fragments arranged by size: 1932 1305 470 24 7 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 275 368 401 779 1501 2176 2209 Size: 130 145 93 33 378 722 675 33 Cuts at: 2209 2571 2750 3505 3553 3690 3738 Size: 362 179 755 48 137 48 Fragments arranged by size: 755 722 675 378 362 179 145 137 130 93 48 48 33 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 2655 3738 Size: 2655 1083 EcoO109I rG'GnC_Cy Cuts at: 0 1287 1405 1419 1682 1863 1864 3655 3738 Size: 1287 118 14 263 181 1 1791 83 Fragments arranged by size: 1791 1287 263 181 118 83 14 1 EcoRI G'AATT_C Cuts at: 0 123 3738 Size: 123 3615 EcoRV GAT'ATC Cuts at: 0 431 3738 Size: 431 3307 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 487 3738 Size: 487 3251 FspI TGC'GCA Cuts at: 0 2053 3738 Size: 2053 1685 HaeII r_GCGC'y Cuts at: 0 1559 1860 3738 Size: 1559 301 1878 Fragments arranged by size: 1878 1559 301 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 238 270 1052 1084 1543 1575 1758 1790 Size: 238 32 782 32 459 32 183 32 Cuts at: 1790 2116 2148 2657 2689 3399 3431 3555 3587 Size: 326 32 509 32 710 32 124 32 Cuts at: 3587 3738 Size: 151 Fragments arranged by size: 782 710 509 459 326 238 183 151 124 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 623 692 1113 3738 Size: 623 69 421 2625 Fragments arranged by size: 2625 623 421 69 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3374 3738 Size: 3374 364 MscI TGG'CCA Cuts at: 0 2083 3014 3738 Size: 2083 931 724 Fragments arranged by size: 2083 931 724 MslI CAynn'nnrTG Cuts at: 0 612 650 931 1921 2500 2621 2717 2828 Size: 612 38 281 990 579 121 96 111 Cuts at: 2828 3738 Size: 910 Fragments arranged by size: 990 910 612 579 281 121 111 96 38 MspA1I CmG'CkG Cuts at: 0 814 1108 1278 1296 1878 2208 3294 3738 Size: 814 294 170 18 582 330 1086 444 Fragments arranged by size: 1086 814 582 444 330 294 170 18 NaeI GCC'GGC Cuts at: 0 1855 2356 3738 Size: 1855 501 1382 Fragments arranged by size: 1855 1382 501 NcoI C'CATG_G Cuts at: 0 1658 1922 3170 3446 3738 Size: 1658 264 1248 276 292 Fragments arranged by size: 1658 1248 292 276 264 NgoMIV G'CCGG_C Cuts at: 0 1853 2354 3738 Size: 1853 501 1384 Fragments arranged by size: 1853 1384 501 NsiI A_TGCA'T Cuts at: 0 255 3738 Size: 255 3483 NspI r_CATG'y Cuts at: 0 145 655 670 2004 2049 2505 3738 Size: 145 510 15 1334 45 456 1233 Fragments arranged by size: 1334 1233 510 456 145 45 15 PasI CC'CwG_GG Cuts at: 0 2920 3738 Size: 2920 818 PciI A'CATG_T Cuts at: 0 651 3738 Size: 651 3087 PflMI CCAn_nnn'nTGG Cuts at: 0 1848 2289 3104 3738 Size: 1848 441 815 634 Fragments arranged by size: 1848 815 634 441 PfoI T'CCnGG_A Cuts at: 0 2186 3251 3738 Size: 2186 1065 487 Fragments arranged by size: 2186 1065 487 PmlI CAC'GTG Cuts at: 0 1452 2580 3504 3738 Size: 1452 1128 924 234 Fragments arranged by size: 1452 1128 924 234 PpuMI rG'GwC_Cy Cuts at: 0 3655 3738 Size: 3655 83 PspOMI G'GGCC_C Cuts at: 0 1201 1863 3738 Size: 1201 662 1875 Fragments arranged by size: 1875 1201 662 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 68 100 3738 Size: 68 32 3638 Fragments arranged by size: 3638 68 32 PstI C_TGCA'G Cuts at: 0 1295 2093 2930 3392 3738 Size: 1295 798 837 462 346 Fragments arranged by size: 1295 837 798 462 346 PvuII CAG'CTG Cuts at: 0 1278 1296 3294 3738 Size: 1278 18 1998 444 Fragments arranged by size: 1998 1278 444 18 RsrII CG'GwC_CG Cuts at: 0 1728 3738 Size: 1728 2010 SacI G_AGCT'C Cuts at: 0 1934 1958 3263 3733 3738 Size: 1934 24 1305 470 5 Fragments arranged by size: 1934 1305 470 24 5 SalI G'TCGA_C Cuts at: 0 621 3738 Size: 621 3117 SapI GCTCTTCn'nnn_ Cuts at: 0 1510 3738 Size: 1510 2228 ScaI AGT'ACT Cuts at: 0 2548 3738 Size: 2548 1190 SfcI C'TryA_G Cuts at: 0 1291 2089 2926 3388 3738 Size: 1291 798 837 462 350 Fragments arranged by size: 1291 837 798 462 350 SgrAI Cr'CCGG_yG Cuts at: 0 2147 3738 Size: 2147 1591 SmaI CCC'GGG Cuts at: 0 3035 3738 Size: 3035 703 SmlI C'TyrA_G Cuts at: 0 1219 2320 2786 3738 Size: 1219 1101 466 952 Fragments arranged by size: 1219 1101 952 466 SnaBI TAC'GTA Cuts at: 0 88 3738 Size: 88 3650 SphI G_CATG'C Cuts at: 0 145 2004 3738 Size: 145 1859 1734 Fragments arranged by size: 1859 1734 145 SspI AAT'ATT Cuts at: 0 2754 3738 Size: 2754 984 StuI AGG'CCT Cuts at: 0 2098 3738 Size: 2098 1640 StyI C'CwwG_G Cuts at: 0 186 839 1658 1691 1922 3131 3170 3446 Size: 186 653 819 33 231 1209 39 276 Cuts at: 3446 3738 Size: 292 Fragments arranged by size: 1209 819 653 292 276 231 186 39 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 869 871 934 1716 3115 3738 Size: 869 2 63 782 1399 623 Fragments arranged by size: 1399 869 782 623 63 2 TatI w'GTAC_w Cuts at: 0 1010 2546 3738 Size: 1010 1536 1192 Fragments arranged by size: 1536 1192 1010 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2953 2985 3029 3061 3738 Size: 2953 32 44 32 677 Fragments arranged by size: 2953 677 44 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1062 1326 2347 3738 Size: 1062 264 1021 1391 Fragments arranged by size: 1391 1062 1021 264 XhoI C'TCGA_G Cuts at: 0 2786 3738 Size: 2786 952 XmaI C'CCGG_G Cuts at: 0 3033 3738 Size: 3033 705 XmnI GAAnn'nnTTC Cuts at: 0 115 467 710 2305 3738 Size: 115 352 243 1595 1433 Fragments arranged by size: 1595 1433 352 243 115 ZraI GAC'GTC Cuts at: 0 582 3401 3738 Size: 582 2819 337 Fragments arranged by size: 2819 582 337 Enzymes that do cut: AarI AatII AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII RsrII SacI SalI SapI ScaI SfcI SgrAI SmaI SmlI SnaBI SphI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AloI AscI AsiSI AvrII BaeI BbeI BcgI BclI BmtI BplI BsaBI BsiWI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI PacI PmeI PpiI PshAI PsiI PspXI PvuI SacII SanDI SbfI SexAI SfiI SfoI SgfI SpeI SrfI SwaI Tth111I XbaI