Restriction map for
pFN21AE1056
(Linear) (Six-base) MAPSORT of: KIEE1056.seq Check: 3064 from: 1 to: 3810
KIEE1056.orf
With 182 enzymes: SgfI *
February 1, 2012 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3625 3810
Size: 3625 185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 923 1625 3065 3810
Size: 323 600 702 1440 745
Fragments arranged by size:
1440 745 702 600 323
AflII C'TTAA_G
Cuts at: 0 1921 3810
Size: 1921 1889
AleI CACnn'nnGTG
Cuts at: 0 604 3810
Size: 604 3206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2417 3810
Size: 2417 1393
AlwNI CAG_nnn'CTG
Cuts at: 0 3430 3810
Size: 3430 380
ApaI G_GGCC'C
Cuts at: 0 410 3810
Size: 410 3400
ApoI r'AATT_y
Cuts at: 0 15 97 147 1174 1209 2715 3810
Size: 15 82 50 1027 35 1506 1095
Fragments arranged by size:
1506 1095 1027 82 50 35 15
AseI AT'TA_AT
Cuts at: 0 1553 2894 3810
Size: 1553 1341 916
Fragments arranged by size:
1553 1341 916
AvaI C'yCGr_G
Cuts at: 0 2072 3152 3665 3810
Size: 2072 1080 513 145
Fragments arranged by size:
2072 1080 513 145
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 733 766 3810
Size: 733 33 3044
Fragments arranged by size:
3044 733 33
BanII G_rGCy'C
Cuts at: 0 410 2340 3810
Size: 410 1930 1470
Fragments arranged by size:
1930 1470 410
BbsI GAAGACnn'nnnn_
Cuts at: 0 1733 3810
Size: 1733 2077
BbvCI CC'TCA_GC
Cuts at: 0 3423 3810
Size: 3423 387
BclI T'GATC_A
Cuts at: 0 615 2739 3810
Size: 615 2124 1071
Fragments arranged by size:
2124 1071 615
BfrBI ATG'CAT
Cuts at: 0 2521 3810
Size: 2521 1289
BglI GCCn_nnn'nGGC
Cuts at: 0 3322 3810
Size: 3322 488
BglII A'GATC_T
Cuts at: 0 3410 3810
Size: 3410 400
Bme1580I G_kGCm'C
Cuts at: 0 410 729 3810
Size: 410 319 3081
Fragments arranged by size:
3081 410 319
BmgBI CAC'GTC
Cuts at: 0 3478 3810
Size: 3478 332
BmrI ACTGGGnnnn_n'
Cuts at: 0 1260 1699 3810
Size: 1260 439 2111
Fragments arranged by size:
2111 1260 439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1889 3810
Size: 1889 1921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1970 3710 3810
Size: 1052 918 1740 100
Fragments arranged by size:
1740 1052 918 100
Bpu10I CC'TnA_GC
Cuts at: 0 3423 3810
Size: 3423 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 821 904 1075 2293 3810
Size: 432 389 83 171 1218 1517
Fragments arranged by size:
1517 1218 432 389 171 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 3310 3810
Size: 3310 500
BsaAI yAC'GTr
Cuts at: 0 2625 2745 3810
Size: 2625 120 1065
Fragments arranged by size:
2625 1065 120
BsaWI w'CCGG_w
Cuts at: 0 778 3810
Size: 778 3032
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 264 294 794 824 1141 1171 3810
Size: 264 30 500 30 317 30 2639
Fragments arranged by size:
2639 500 317 264 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 437 1397 3671 3707 3761 3809 3810
Size: 437 960 2274 36 54 48 1
Fragments arranged by size:
2274 960 437 54 48 36 1
BseYI C'CCAG_C
Cuts at: 0 508 663 2697 3810
Size: 508 155 2034 1113
Fragments arranged by size:
2034 1113 508 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2349 3653 3810
Size: 2349 1304 157
Fragments arranged by size:
2349 1304 157
BsiHKAI G_wGCw'C
Cuts at: 0 1481 2340 2825 3810
Size: 1481 859 485 985
Fragments arranged by size:
1481 985 859 485
BsmI GAATG_Cn'
Cuts at: 0 1502 1733 2058 3810
Size: 1502 231 325 1752
Fragments arranged by size:
1752 1502 325 231
Bsp1286I G_dGCh'C
Cuts at: 0 410 729 1481 2340 2825 3810
Size: 410 319 752 859 485 985
Fragments arranged by size:
985 859 752 485 410 319
BspEI T'CCGG_A
Cuts at: 0 778 3810
Size: 778 3032
BspHI T'CATG_A
Cuts at: 0 1125 3279 3810
Size: 1125 2154 531
Fragments arranged by size:
2154 1125 531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2668 3360 3625 3810
Size: 2668 692 265 185
Fragments arranged by size:
2668 692 265 185
BsrDI GCAATG_nn'
Cuts at: 0 183 2389 2645 3810
Size: 183 2206 256 1165
Fragments arranged by size:
2206 1165 256 183
BssSI C'ACGA_G
Cuts at: 0 683 3810
Size: 683 3127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 855 2527 3243 3810
Size: 855 1672 716 567
Fragments arranged by size:
1672 855 716 567
BstBI TT'CG_AA
Cuts at: 0 210 953 3147 3810
Size: 210 743 2194 663
Fragments arranged by size:
2194 743 663 210
BstEII G'GTnAC_C
Cuts at: 0 995 3810
Size: 995 2815
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3232 3810
Size: 3232 578
BstYI r'GATC_y
Cuts at: 0 578 906 3410 3727 3810
Size: 578 328 2504 317 83
Fragments arranged by size:
2504 578 328 317 83
BtgI C'CryG_G
Cuts at: 0 323 3317 3810
Size: 323 2994 493
Fragments arranged by size:
2994 493 323
BtsI GCAGTG_nn'
Cuts at: 0 80 2216 3576 3810
Size: 80 2136 1360 234
Fragments arranged by size:
2136 1360 234 80
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2124 2159 3810
Size: 2124 35 1651
Fragments arranged by size:
2124 1651 35
DraI TTT'AAA
Cuts at: 0 503 3810
Size: 503 3307
EaeI y'GGCC_r
Cuts at: 0 2295 2439 2450 3810
Size: 2295 144 11 1360
Fragments arranged by size:
2295 1360 144 11
EarI CTCTTCn'nnn_
Cuts at: 0 341 1382 2218 3786 3810
Size: 341 1041 836 1568 24
Fragments arranged by size:
1568 1041 836 341 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 251 3810
Size: 251 3559
EcoICRI GAG'CTC
Cuts at: 0 2338 3810
Size: 2338 1472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 923 1052 1625 1970 3065 3710 3810
Size: 323 600 129 573 345 1095 645 100
Fragments arranged by size:
1095 645 600 573 345 323 129 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3713 3810
Size: 3713 97
EcoO109I rG'GnC_Cy
Cuts at: 0 331 383 610 1048 3706 3761 3810
Size: 331 52 227 438 2658 55 49
Fragments arranged by size:
2658 438 331 227 55 52 49
HaeII r_GCGC'y
Cuts at: 0 3608 3810
Size: 3608 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 2621 2653 3135 3167 3810
Size: 430 32 2159 32 482 32 643
Fragments arranged by size:
2159 643 482 430 32 32 32
HincII GTy'rAC
Cuts at: 0 198 2543 2924 3810
Size: 198 2345 381 886
Fragments arranged by size:
2345 886 381 198
HindIII A'AGCT_T
Cuts at: 0 770 3810
Size: 770 3040
HpaI GTT'AAC
Cuts at: 0 198 3810
Size: 198 3612
MfeI C'AATT_G
Cuts at: 0 1668 1876 2459 3810
Size: 1668 208 583 1351
Fragments arranged by size:
1668 1351 583 208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1094 2091 3350 3810
Size: 1034 60 997 1259 460
Fragments arranged by size:
1259 1034 997 460 60
MscI TGG'CCA
Cuts at: 0 2441 2452 3810
Size: 2441 11 1358
Fragments arranged by size:
2441 1358 11
MslI CAynn'nnrTG
Cuts at: 0 139 604 1738 3810
Size: 139 465 1134 2072
Fragments arranged by size:
2072 1134 465 139
MspA1I CmG'CkG
Cuts at: 0 663 855 3427 3810
Size: 663 192 2572 383
Fragments arranged by size:
2572 663 383 192
NcoI C'CATG_G
Cuts at: 0 323 3317 3810
Size: 323 2994 493
Fragments arranged by size:
2994 493 323
NsiI A_TGCA'T
Cuts at: 0 2523 3810
Size: 2523 1287
NspI r_CATG'y
Cuts at: 0 1650 1872 3810
Size: 1650 222 1938
Fragments arranged by size:
1938 1650 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 247 2026 2448 3810
Size: 247 1779 422 1362
Fragments arranged by size:
1779 1362 422 247
PfoI T'CCnGG_A
Cuts at: 0 1947 3810
Size: 1947 1863
PpuMI rG'GwC_Cy
Cuts at: 0 331 610 3810
Size: 331 279 3200
Fragments arranged by size:
3200 331 279
PshAI GACnn'nnGTC
Cuts at: 0 3181 3810
Size: 3181 629
PsiI TTA'TAA
Cuts at: 0 128 3810
Size: 128 3682
PspOMI G'GGCC_C
Cuts at: 0 406 3810
Size: 406 3404
PspXI vC'TCGA_Gb
Cuts at: 0 3152 3810
Size: 3152 658
PstI C_TGCA'G
Cuts at: 0 3197 3810
Size: 3197 613
PvuII CAG'CTG
Cuts at: 0 663 855 3427 3810
Size: 663 192 2572 383
Fragments arranged by size:
2572 663 383 192
SacI G_AGCT'C
Cuts at: 0 2340 3810
Size: 2340 1470
SapI GCTCTTCn'nnn_
Cuts at: 0 1382 3810
Size: 1382 2428
ScaI AGT'ACT
Cuts at: 0 1577 3810
Size: 1577 2233
SfcI C'TryA_G
Cuts at: 0 290 1410 1469 3193 3564 3651 3699 3720
Size: 290 1120 59 1724 371 87 48 21
Cuts at: 3720 3810
Size: 90
Fragments arranged by size:
1724 1120 371 290 90 87 59 48
21
SmlI C'TyrA_G
Cuts at: 0 447 836 883 1090 1921 2072 2272 3152
Size: 447 389 47 207 831 151 200 880
Cuts at: 3152 3810
Size: 658
Fragments arranged by size:
880 831 658 447 389 207 200 151
47
SpeI A'CTAG_T
Cuts at: 0 1027 3810
Size: 1027 2783
SspI AAT'ATT
Cuts at: 0 562 1660 1717 2142 3810
Size: 562 1098 57 425 1668
Fragments arranged by size:
1668 1098 562 425 57
StyI C'CwwG_G
Cuts at: 0 323 530 582 991 1021 2120 3317 3810
Size: 323 207 52 409 30 1099 1197 493
Fragments arranged by size:
1197 1099 493 409 323 207 52 30
SwaI ATTT'AAAT
Cuts at: 0 503 3810
Size: 503 3307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 434 616 970 1313 2779 3810
Size: 434 182 354 343 1466 1031
Fragments arranged by size:
1466 1031 434 354 343 182
TatI w'GTAC_w
Cuts at: 0 596 1465 1575 1925 3454 3810
Size: 596 869 110 350 1529 356
Fragments arranged by size:
1529 869 596 356 350 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 794 826 1245 1277 3810
Size: 794 32 419 32 2533
Fragments arranged by size:
2533 794 419 32 32
XbaI T'CTAG_A
Cuts at: 0 480 1275 3415 3810
Size: 480 795 2140 395
Fragments arranged by size:
2140 795 480 395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1010 3324 3748 3810
Size: 1010 2314 424 62
Fragments arranged by size:
2314 1010 424 62
XhoI C'TCGA_G
Cuts at: 0 2072 3152 3810
Size: 2072 1080 658
Fragments arranged by size:
2072 1080 658
XmnI GAAnn'nnTTC
Cuts at: 0 236 1718 2212 3143 3810
Size: 236 1482 494 931 667
Fragments arranged by size:
1482 931 667 494 236
Enzymes that do cut:
AarI AcuI AflII AleI AlfI AlwNI ApaI ApoI
AseI AvaI BaeI BanII BbsI BbvCI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtsI CspCI
DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI
MslI MspA1I NcoI NsiI NspI PflMI PfoI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuII SacI SapI
ScaI SfcI SmlI SpeI SspI StyI SwaI TaqII
TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AloI ApaLI AscI AsiSI AvrII BamHI BanI BbeI
BcgI BciVI BlpI BmtI BsaBI BsaHI BsiEI BsiWI
BsmBI BsrBI BsrFI BsrGI BssHII BstZ17I Bsu36I BtgZI
ClaI DraIII DrdI EagI EcoRI EcoRV FalI FseI
FspI FspAI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI Tth111I XmaI ZraI