(Circular) (Six-base) MAPSORT of: pFN21AB8640.seq Check: 9686 from: 1 to: 5314 pFN21AB8640 5314 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2190 3124 278 Size: 53 83 186 936 654 934 2468 Fragments arranged by size: 2468 936 934 654 186 83 53 AccI GT'mk_AC Cuts at: 1937 2160 2280 2657 1937 Size: 223 120 377 4594 Fragments arranged by size: 4594 377 223 120 Acc65I G'GTAC_C Cuts at: 2635 2635 Size: 5314 AclI AA'CG_TT Cuts at: 1563 3441 3814 1563 Size: 1878 373 3063 Fragments arranged by size: 3063 1878 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3377 4761 5029 1632 Size: 1745 1384 268 1917 Fragments arranged by size: 1917 1745 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5117 AflIII A'CryG_T Cuts at: 1203 4213 5190 1203 Size: 3010 977 1327 Fragments arranged by size: 3010 1327 977 AgeI A'CCGG_T Cuts at: 4145 4145 Size: 5314 AhdI GACnn_n'nnGTC Cuts at: 4043 4043 Size: 5314 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5314 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5314 AlwNI CAG_nnn'CTG Cuts at: 2410 2457 3057 4629 2410 Size: 47 600 1572 3095 Fragments arranged by size: 3095 1572 600 47 ApaLI G'TGCA_C Cuts at: 3371 4527 3371 Size: 1156 4158 ApoI r'AATT_y Cuts at: 1493 2623 2849 3001 5110 1493 Size: 1130 226 152 2109 1697 Fragments arranged by size: 2109 1697 1130 226 152 AscI GG'CGCG_CC Cuts at: 2358 2358 Size: 5314 AseI AT'TA_AT Cuts at: 160 1032 3868 160 Size: 872 2836 1606 Fragments arranged by size: 2836 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5314 AvaI C'yCGr_G Cuts at: 1870 1943 2041 2093 2203 2210 2474 2639 1870 Size: 73 98 52 110 7 264 165 4545 Fragments arranged by size: 4545 264 165 110 98 73 52 7 BamHI G'GATC_C Cuts at: 2644 3011 2644 Size: 367 4947 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2153 2635 4090 618 Size: 325 217 627 249 117 482 1455 1842 Fragments arranged by size: 1842 1455 627 482 325 249 217 117 BanII G_rGCy'C Cuts at: 1391 2210 2633 1391 Size: 819 423 4072 Fragments arranged by size: 4072 819 423 BbeI G_GCGC'C Cuts at: 2040 2157 2040 Size: 117 5197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4343 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3505 3539 1903 Size: 34 1568 34 3678 Fragments arranged by size: 3678 1568 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3206 4416 3206 Size: 1210 4104 BclI T'GATC_A Cuts at: 1548 1548 Size: 5314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3925 136 Size: 107 122 71 631 605 2253 1525 Fragments arranged by size: 2253 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5309 5309 Size: 5314 BlpI GC'TnA_GC Cuts at: 2731 2731 Size: 5314 Bme1580I G_kGCm'C Cuts at: 1790 3375 4531 1790 Size: 1585 1156 2573 Fragments arranged by size: 2573 1585 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3045 4003 442 Size: 416 53 83 399 24 1628 958 1753 Fragments arranged by size: 1753 1628 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5314 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2266 3974 1239 Size: 1027 1708 2579 Fragments arranged by size: 2579 1708 1027 Bpu10I CC'TnA_GC Cuts at: 2238 2238 Size: 5314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2188 3439 4304 4602 2188 Size: 1251 865 298 2900 Fragments arranged by size: 2900 1251 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2336 3977 882 Size: 617 837 1641 2219 Fragments arranged by size: 2219 1641 837 617 BsaAI yAC'GTr Cuts at: 493 1204 2314 2503 3105 493 Size: 711 1110 189 602 2702 Fragments arranged by size: 2702 1110 711 602 189 BsaBI GATnn'nnATC Cuts at: 5308 5308 Size: 5314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 2154 2187 2265 Size: 53 83 186 936 504 117 33 78 Cuts at: 2265 3121 3503 4138 275 Size: 856 382 635 1451 Fragments arranged by size: 1451 936 856 635 504 382 186 117 83 78 53 33 BsaWI w'CCGG_w Cuts at: 2218 3747 4145 4419 4566 4977 2218 Size: 1529 398 274 147 411 2555 Fragments arranged by size: 2555 1529 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5089 Fragments arranged by size: 5089 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 1186 Size: 1051 4263 BseYI C'CCAG_C Cuts at: 4517 5013 4517 Size: 496 4818 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2541 1163 Size: 573 805 3936 Fragments arranged by size: 3936 805 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2764 3525 4553 1540 Size: 275 186 763 761 1028 2301 Fragments arranged by size: 2301 1028 763 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 2483 3375 3460 4531 2277 Size: 206 892 85 1071 3060 Fragments arranged by size: 3060 1071 892 206 85 BsmI GAATG_Cn' Cuts at: 2825 2918 2825 Size: 93 5221 BsmBI CGTCTCn'nnnn_ Cuts at: 2516 2516 Size: 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2277 2483 2633 3375 3460 4531 Size: 399 420 67 206 150 742 85 1071 Cuts at: 4531 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 420 399 206 150 85 67 BspEI T'CCGG_A Cuts at: 2218 4977 2218 Size: 2759 2555 BspHI T'CATG_A Cuts at: 3203 4933 3203 Size: 1730 3584 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2669 844 Size: 1825 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3201 1394 Size: 429 1378 3507 Fragments arranged by size: 3507 1378 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3809 3983 65 Size: 1183 2561 174 1396 Fragments arranged by size: 2561 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 2350 3958 4145 1700 Size: 110 293 247 1608 187 2869 Fragments arranged by size: 2869 1608 293 247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5314 BssHII G'CGCG_C Cuts at: 1925 2358 2428 2430 2545 2547 2549 2597 1925 Size: 433 70 2 115 2 2 48 4642 Fragments arranged by size: 4642 433 115 70 48 2 2 2 BssSI C'ACGA_G Cuts at: 2378 3374 4386 2378 Size: 996 1012 3306 Fragments arranged by size: 3306 1012 996 BstAPI GCAn_nnn'nTGC Cuts at: 794 2410 2506 794 Size: 1616 96 3602 Fragments arranged by size: 3602 1616 96 BstBI TT'CG_AA Cuts at: 4118 4118 Size: 5314 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5314 BstXI CCAn_nnnn'nTGG Cuts at: 2380 2380 Size: 5314 BstYI r'GATC_y Cuts at: 1972 2644 3011 3396 3413 4865 4951 4963 5309 Size: 672 367 385 17 1452 86 12 346 Cuts at: 5309 1972 Size: 1977 Fragments arranged by size: 1977 1452 672 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2109 2136 2423 513 Size: 552 939 105 27 287 3404 Fragments arranged by size: 3404 939 552 287 105 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2320 487 Size: 683 56 1094 3481 Fragments arranged by size: 3481 1094 683 56 BtsI GCAGTG_nn' Cuts at: 2830 3624 3644 2830 Size: 794 20 4500 Fragments arranged by size: 4500 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5279 DraI TTT'AAA Cuts at: 2618 2618 Size: 5314 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2156 2186 4321 809 Size: 507 840 30 2135 1802 Fragments arranged by size: 2135 1802 840 507 30 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2058 2228 Size: 54 1425 50 275 18 195 33 170 Cuts at: 2228 2436 2761 3650 5279 8 Size: 208 325 889 1629 43 Fragments arranged by size: 1629 1425 889 325 275 208 195 170 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2761 1537 Size: 275 949 4090 Fragments arranged by size: 4090 949 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2519 3244 1353 Size: 1166 725 3423 Fragments arranged by size: 3423 1166 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2057 2473 3901 4275 4421 387 Size: 1064 138 468 416 1428 374 146 1280 Fragments arranged by size: 1428 1280 1064 468 416 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2266 3377 3974 4761 5029 1239 Size: 393 634 1111 597 787 268 1524 Fragments arranged by size: 1524 1111 787 634 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2320 2320 Size: 5314 EcoRI G'AATT_C Cuts at: 2623 3001 2623 Size: 378 4936 FspI TGC'GCA Cuts at: 2450 3820 2450 Size: 1370 3944 HaeII r_GCGC'y Cuts at: 1678 2040 2101 2157 2372 2464 2588 4461 1678 Size: 362 61 56 215 92 124 1873 2531 Fragments arranged by size: 2531 1873 362 215 124 92 61 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3955 3987 4029 4061 1373 Size: 32 135 32 2383 32 42 32 2626 Fragments arranged by size: 2626 2383 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2658 2904 669 Size: 988 281 720 246 3079 Fragments arranged by size: 3079 988 720 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5314 HpaI GTT'AAC Cuts at: 2904 2904 Size: 5314 KasI G'GCGC_C Cuts at: 2036 2153 2036 Size: 117 5197 KpnI G_GTAC'C Cuts at: 2639 2639 Size: 5314 MfeI C'AATT_G Cuts at: 2913 2913 Size: 5314 MluI A'CGCG_T Cuts at: 5190 5190 Size: 5314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4428 4612 1305 Size: 3123 184 2007 Fragments arranged by size: 3123 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2230 10 Size: 54 1425 343 398 3094 Fragments arranged by size: 3094 1425 398 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2378 3272 3631 3790 518 Size: 648 1212 894 359 159 2042 Fragments arranged by size: 2042 1212 894 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2138 2407 2425 2730 3407 4555 4800 1606 Size: 532 269 18 305 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 532 305 269 245 18 NaeI GCC'GGC Cuts at: 2352 2352 Size: 5314 NarI GG'CG_CC Cuts at: 2037 2154 2037 Size: 117 5197 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3823 Fragments arranged by size: 3823 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5314 NgoMIV G'CCGG_C Cuts at: 2350 2350 Size: 5314 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5314 NotI GC'GGCC_GC Cuts at: 2761 2761 Size: 5314 NspI r_CATG'y Cuts at: 1132 1229 2672 4217 1132 Size: 97 1443 1545 2229 Fragments arranged by size: 2229 1545 1443 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5314 PciI A'CATG_T Cuts at: 4213 4213 Size: 5314 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5314 PmeI GTTT'AAAC Cuts at: 2618 2618 Size: 5314 PmlI CAC'GTG Cuts at: 2503 2503 Size: 5314 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3383 3415 4896 4928 3383 Size: 32 1481 32 3769 Fragments arranged by size: 3769 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5314 PsiI TTA'TAA Cuts at: 2884 2884 Size: 5314 PspXI vC'TCGA_Gb Cuts at: 2203 2474 2203 Size: 271 5043 PstI C_TGCA'G Cuts at: 830 2294 2580 2606 2666 830 Size: 1464 286 26 60 3478 Fragments arranged by size: 3478 1464 286 60 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5314 RsrII CG'GwC_CG Cuts at: 2420 2420 Size: 5314 SacII CC_GC'GG Cuts at: 2139 2426 2139 Size: 287 5027 SalI G'TCGA_C Cuts at: 1936 2656 1936 Size: 720 4594 SbfI CC_TGCA'GG Cuts at: 2580 2666 2580 Size: 86 5228 ScaI AGT'ACT Cuts at: 3562 5274 3562 Size: 1712 3602 SfcI C'TryA_G Cuts at: 826 1045 2290 2576 2602 2662 3797 4478 4669 Size: 219 1245 286 26 60 1135 681 191 Cuts at: 4669 826 Size: 1471 Fragments arranged by size: 1471 1245 1135 681 286 219 191 60 26 SfoI GGC'GCC Cuts at: 2038 2155 2038 Size: 117 5197 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5314 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5314 SmaI CCC'GGG Cuts at: 1872 2043 2212 2641 1872 Size: 171 169 429 4545 Fragments arranged by size: 4545 429 171 169 SmlI C'TyrA_G Cuts at: 820 1017 1943 2167 2203 2474 3418 4319 4581 Size: 197 926 224 36 271 944 901 262 Cuts at: 4581 820 Size: 1553 Fragments arranged by size: 1553 944 926 901 271 262 224 197 36 SnaBI TAC'GTA Cuts at: 493 3105 493 Size: 2612 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5314 SphI G_CATG'C Cuts at: 1132 2672 1132 Size: 1540 3774 SspI AAT'ATT Cuts at: 5 52 3238 5 Size: 47 3186 2081 Fragments arranged by size: 3186 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5314 StyI C'CwwG_G Cuts at: 513 1065 2004 2753 513 Size: 552 939 749 3074 Fragments arranged by size: 3074 939 749 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3341 3358 3511 3696 4141 325 Size: 1255 1761 17 153 185 445 1498 Fragments arranged by size: 1761 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2490 3560 5272 Size: 274 80 33 51 1443 513 1070 1712 Cuts at: 5272 96 Size: 138 Fragments arranged by size: 1712 1443 1070 513 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5274 5306 5274 Size: 32 5282 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5314 XbaI T'CTAG_A Cuts at: 2650 2650 Size: 5314 XhoI C'TCGA_G Cuts at: 1943 2203 2474 1943 Size: 260 271 4783 Fragments arranged by size: 4783 271 260 XmaI C'CCGG_G Cuts at: 1870 2041 2210 2639 1870 Size: 171 169 429 4545 Fragments arranged by size: 4545 429 171 169 XmnI GAAnn'nnTTC Cuts at: 3443 3443 Size: 5314 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2188 3122 276 Size: 53 83 186 936 654 934 2468 Fragments arranged by size: 2468 936 934 654 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PsrI PvuII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI