##################################
# NO CUT ENZYMES (  228 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssKI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       ItaI        KasI        KpnI        
KspI        MaeI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        TseI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AfaI           62  GTAC                 GT^AC
AseI          148  ATTAAT               AT^TAAT
AsnI          148  ATTAAT               AT^TAAT
BclI          206  TGATCA               T^GATCA
BsrDI          76  GCAATG               GCAATG(2/0)
BsrI           67  ACTGG                ACTGG(1/-1)
BssSI         167  CTCGTG               CACGAG(-5/-1)
BstXI          65  CCACTGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         207  GATC                 ^GATC
EarI           93  CTCTTC               CTCTTC(1/4)
FbaI          206  TGATCA               T^GATCA
HphI          183  GGTGA                GGTGA(8/7)
Ksp632I        93  CTCTTC               CTCTTC(1/4)
MboI          207  GATC                 ^GATC
MslI           66  CACTGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         207  GATC                 ^GATC
PshBI         148  ATTAAT               AT^TAAT
RsaI           62  GTAC                 GT^AC
Sau3AI        207  GATC                 ^GATC
TaqI          231  TCGA                 T^CGA
Tsp45I        103  GTCAC                ^GT[GC]AC
TspRI          66  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       231  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

MaeIII        103  GTCAC                ^GT[ACGT]AC
              113  GTTAC               
MboII          94  TCTTC                GAAGA(8/7)
               97  TCTTC               
MnlI           92  CCTC                 CCTC(7/6)
              181  GAGG                
MseI          149  TTAA                 T^TAA
              214  TTAA                
SfaNI         186  GATGC                GCATC(5/9)
               55  GCATC               
Tru9I         149  TTAA                 T^TAA
              214  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

Tsp509I