(Circular) (Six-base) MAPSORT of: pFN21AE6352.seq Check: 128 from: 1 to: 5815 pFN21AE6352 5815 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2011 2011 Size: 5815 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3878 5262 5530 1632 Size: 2246 1384 268 1917 Fragments arranged by size: 2246 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 2448 2532 4714 5691 1203 Size: 1245 84 2182 977 1327 Fragments arranged by size: 2182 1327 1245 977 84 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 2630 1166 Size: 1464 4351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 3073 3558 5130 3073 Size: 485 1572 3758 Fragments arranged by size: 3758 1572 485 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 2403 3092 3124 3350 3502 5611 1493 Size: 910 689 32 226 152 2109 1697 Fragments arranged by size: 2109 1697 910 689 226 152 32 AseI AT'TA_AT Cuts at: 160 1032 4369 160 Size: 872 3337 1606 Fragments arranged by size: 3337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 3140 1870 Size: 73 1197 4545 Fragments arranged by size: 4545 1197 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2594 2792 3136 4591 618 Size: 325 217 627 807 198 344 1455 1842 Fragments arranged by size: 1842 1455 807 627 344 325 217 198 BanII G_rGCy'C Cuts at: 1391 2889 3134 1391 Size: 1498 245 4072 Fragments arranged by size: 4072 1498 245 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3066 928 Size: 971 1167 3677 Fragments arranged by size: 3677 1167 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4006 4040 1903 Size: 34 2069 34 3678 Fragments arranged by size: 3678 2069 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BfrBI ATG'CAT Cuts at: 3106 3106 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4426 136 Size: 107 122 71 631 605 2754 1525 Fragments arranged by size: 2754 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5810 5810 Size: 5815 BlpI GC'TnA_GC Cuts at: 2813 3232 2813 Size: 419 5396 Bme1580I G_kGCm'C Cuts at: 1790 2599 2678 3876 5032 1790 Size: 809 79 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 809 79 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3546 4504 442 Size: 416 53 83 399 24 2129 958 1753 Fragments arranged by size: 2129 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2198 1056 Size: 1142 4673 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2695 2695 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2789 4475 1239 Size: 1550 1686 2579 Fragments arranged by size: 2579 1686 1550 Bpu10I CC'TnA_GC Cuts at: 2331 2331 Size: 5815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2844 3940 4805 5103 2844 Size: 1096 865 298 3556 Fragments arranged by size: 3556 1096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2449 3606 493 Size: 711 1245 1157 2702 Fragments arranged by size: 2702 1245 1157 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 3622 4004 4639 Size: 53 83 186 936 477 1612 382 635 Cuts at: 4639 275 Size: 1451 Fragments arranged by size: 1612 1451 936 635 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5590 Fragments arranged by size: 5590 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5815 BseYI C'CCAG_C Cuts at: 2618 2653 5018 5514 2618 Size: 35 2365 496 2919 Fragments arranged by size: 2919 2365 496 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2720 1163 Size: 573 984 4258 Fragments arranged by size: 4258 984 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3265 4026 5054 1540 Size: 275 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3876 3961 5032 3876 Size: 85 1071 4659 Fragments arranged by size: 4659 1071 85 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 BsmBI CGTCTCn'nnnn_ Cuts at: 2462 2462 Size: 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2599 2678 2889 3134 3876 3961 5032 Size: 399 809 79 211 245 742 85 1071 Cuts at: 5032 1391 Size: 2174 Fragments arranged by size: 2174 1071 809 742 399 245 211 85 79 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 3084 3704 5434 3084 Size: 620 1730 3465 Fragments arranged by size: 3465 1730 620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2011 2658 3170 844 Size: 1167 647 512 3489 Fragments arranged by size: 3489 1167 647 512 BsrBI CCG'CTC Cuts at: 1394 1823 3702 1394 Size: 429 1879 3507 Fragments arranged by size: 3507 1879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2935 4310 4484 65 Size: 1183 1687 1375 174 1396 Fragments arranged by size: 1687 1396 1375 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4459 4646 1700 Size: 110 2649 187 2869 Fragments arranged by size: 2869 2649 187 110 BsrGI T'GTAC_A Cuts at: 96 2721 96 Size: 2625 3190 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5815 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 2623 1184 Size: 1439 4376 BstXI CCAn_nnnn'nTGG Cuts at: 2023 2421 2560 2023 Size: 398 139 5278 Fragments arranged by size: 5278 398 139 BstYI r'GATC_y Cuts at: 1972 2410 3145 3512 3897 3914 5366 5452 5464 Size: 438 735 367 385 17 1452 86 12 Cuts at: 5464 5810 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 735 438 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2628 2796 2802 513 Size: 552 939 624 168 6 3526 Fragments arranged by size: 3526 939 624 552 168 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5076 Fragments arranged by size: 5076 683 56 BtsI GCAGTG_nn' Cuts at: 3331 4125 4145 3331 Size: 794 20 5001 Fragments arranged by size: 5001 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DraIII CAC_nnn'GTG Cuts at: 2065 2065 Size: 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 2799 3075 Size: 54 1425 50 275 18 415 554 276 Cuts at: 3075 3262 4151 5780 8 Size: 187 889 1629 43 Fragments arranged by size: 1629 1425 889 554 415 276 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3745 1353 Size: 2392 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4402 4776 4922 387 Size: 1064 138 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2789 3878 4475 5262 5530 1239 Size: 393 1157 1089 597 787 268 1524 Fragments arranged by size: 1524 1157 1089 787 597 393 268 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 FspI TGC'GCA Cuts at: 2461 4321 2461 Size: 1860 3955 HaeII r_GCGC'y Cuts at: 1678 4962 1678 Size: 3284 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 4456 4488 4530 Size: 32 135 32 950 32 1902 32 42 Cuts at: 4530 4562 1373 Size: 32 2626 Fragments arranged by size: 2626 1902 950 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2029 3159 3405 669 Size: 988 281 91 1130 246 3079 Fragments arranged by size: 3079 1130 988 281 246 91 HindIII A'AGCT_T Cuts at: 748 2810 748 Size: 2062 3753 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 3054 3414 3054 Size: 360 5455 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4929 5113 1305 Size: 3624 184 2007 Fragments arranged by size: 3624 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3993 Fragments arranged by size: 3993 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 3773 4132 4291 518 Size: 648 1464 1143 359 159 2042 Fragments arranged by size: 2042 1464 1143 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2798 3231 3908 5056 5301 1606 Size: 1192 433 677 1148 245 2120 Fragments arranged by size: 2120 1192 1148 677 433 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2802 513 Size: 552 939 798 3526 Fragments arranged by size: 3526 939 798 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NheI G'CTAG_C Cuts at: 1052 2194 1052 Size: 1142 4673 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NsiI A_TGCA'T Cuts at: 3108 3108 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 2536 3173 4718 1132 Size: 97 1307 637 1545 2229 Fragments arranged by size: 2229 1545 1307 637 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 2532 4714 2532 Size: 2182 3633 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5815 PfoI T'CCnGG_A Cuts at: 2685 2685 Size: 5815 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PstI C_TGCA'G Cuts at: 830 3167 830 Size: 2337 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 SacII CC_GC'GG Cuts at: 2799 2799 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 2520 4063 5775 2520 Size: 1543 1712 2560 Fragments arranged by size: 2560 1712 1543 SfcI C'TryA_G Cuts at: 826 1045 3163 4298 4979 5170 826 Size: 219 2118 1135 681 191 1471 Fragments arranged by size: 2118 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2859 3919 4820 5082 820 Size: 197 926 916 1060 901 262 1553 Fragments arranged by size: 1553 1060 926 916 901 262 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 2911 3739 5 Size: 47 2859 828 2081 Fragments arranged by size: 2859 2081 828 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 2004 2802 3254 513 Size: 552 939 798 452 3074 Fragments arranged by size: 3074 939 798 552 452 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2229 2671 3842 3859 4012 4197 4642 Size: 1255 649 442 1171 17 153 185 445 Cuts at: 4642 325 Size: 1498 Fragments arranged by size: 1498 1255 1171 649 445 442 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2518 2721 4061 Size: 274 80 33 51 1443 541 203 1340 Cuts at: 4061 5773 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1340 541 274 203 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 5783 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2606 2606 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 3063 3944 3063 Size: 881 4934 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI