(Circular) (Six-base) MAPSORT of: pFN21AB6560.seq Check: 1061 from: 1 to: 5731 pFN21AB6560 5731 bp With 182 enzymes: SgfI * July 29, 2009 14:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3074 1937 Size: 1137 4594 Acc65I G'GTAC_C Cuts at: 3052 3052 Size: 5731 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3794 5178 5446 1632 Size: 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 268 AfeI AGC'GCT Cuts at: 2119 2119 Size: 5731 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 2074 2140 4630 5607 1203 Size: 871 66 2490 977 1327 Fragments arranged by size: 2490 1327 977 871 66 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 2079 1166 Size: 913 4818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2490 2522 2490 Size: 32 5699 AlwNI CAG_nnn'CTG Cuts at: 3023 3474 5046 3023 Size: 451 1572 3708 Fragments arranged by size: 3708 1572 451 ApaI G_GGCC'C Cuts at: 2405 2405 Size: 5731 ApaLI G'TGCA_C Cuts at: 3788 4944 3788 Size: 1156 4575 ApoI r'AATT_y Cuts at: 1493 3040 3266 3418 5527 1493 Size: 1547 226 152 2109 1697 Fragments arranged by size: 2109 1697 1547 226 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 3056 1870 Size: 73 1113 4545 Fragments arranged by size: 4545 1113 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2868 2901 2868 Size: 33 5698 BamHI G'GATC_C Cuts at: 3061 3428 3061 Size: 367 5364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2125 2332 3052 4507 618 Size: 325 217 627 338 207 720 1455 1842 Fragments arranged by size: 1842 1455 720 627 338 325 217 207 BanII G_rGCy'C Cuts at: 1391 2223 2405 2456 2913 3050 1391 Size: 832 182 51 457 137 4072 Fragments arranged by size: 4072 832 457 182 137 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4760 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3922 3956 1903 Size: 34 1985 34 3678 Fragments arranged by size: 3678 1985 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 2352 1548 Size: 804 4927 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4342 136 Size: 107 122 71 631 605 2670 1525 Fragments arranged by size: 2670 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 2907 3148 2907 Size: 241 5490 Bme1580I G_kGCm'C Cuts at: 1790 2074 2405 2520 3792 4948 1790 Size: 284 331 115 1272 1156 2573 Fragments arranged by size: 2573 1272 1156 331 284 115 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3462 4420 442 Size: 416 53 83 399 24 2045 958 1753 Fragments arranged by size: 2045 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4391 1239 Size: 3152 2579 Bpu10I CC'TnA_GC Cuts at: 2217 2217 Size: 5731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2115 3856 4721 5019 2115 Size: 1741 865 298 2827 Fragments arranged by size: 2827 1741 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2570 2857 4394 882 Size: 617 1071 287 1537 2219 Fragments arranged by size: 2219 1537 1071 617 287 BsaAI yAC'GTr Cuts at: 493 1204 2077 2143 3522 493 Size: 711 873 66 1379 2702 Fragments arranged by size: 2702 1379 873 711 66 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3538 3920 4555 275 Size: 53 83 186 936 2005 382 635 1451 Fragments arranged by size: 2005 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4164 4562 4836 4983 5394 4164 Size: 398 274 147 411 4501 Fragments arranged by size: 4501 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2127 2157 2297 2327 2412 Size: 30 165 30 724 30 140 30 85 Cuts at: 2412 2442 2490 2520 1178 Size: 30 48 30 4389 Fragments arranged by size: 4389 724 165 140 85 48 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2869 1186 Size: 1683 4048 BseYI C'CCAG_C Cuts at: 2114 2269 2336 2519 2994 4934 5430 2114 Size: 155 67 183 475 1940 496 2415 Fragments arranged by size: 2415 1940 496 475 183 155 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2399 3181 3942 4970 1540 Size: 275 186 398 782 761 1028 2301 Fragments arranged by size: 2301 1028 782 761 398 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 2456 2940 3792 3877 4948 2223 Size: 233 484 852 85 1071 3006 Fragments arranged by size: 3006 1071 852 484 233 85 BsmI GAATG_Cn' Cuts at: 3242 3335 3242 Size: 93 5638 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2223 2405 2456 2520 2913 2940 Size: 399 284 149 182 51 64 393 27 Cuts at: 2940 3050 3792 3877 4948 1391 Size: 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 284 182 149 110 85 64 51 27 BspEI T'CCGG_A Cuts at: 5394 5394 Size: 5731 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3086 844 Size: 2242 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3618 1394 Size: 429 1795 3507 Fragments arranged by size: 3507 1795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4226 4400 65 Size: 1183 2978 174 1396 Fragments arranged by size: 2978 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4375 4562 1700 Size: 110 2565 187 2869 Fragments arranged by size: 2869 2565 187 110 BsrGI T'GTAC_A Cuts at: 96 2190 96 Size: 2094 3637 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 3791 4803 3791 Size: 1012 4719 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2122 Size: 5731 BstYI r'GATC_y Cuts at: 1972 2606 2960 3061 3428 3813 3830 5282 5368 Size: 634 354 101 367 385 17 1452 86 Cuts at: 5368 5380 5726 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 634 385 367 354 346 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2784 2784 Size: 5731 BtgI C'CryG_G Cuts at: 513 1065 2019 2406 2569 2602 513 Size: 552 954 387 163 33 3642 Fragments arranged by size: 3642 954 552 387 163 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4992 Fragments arranged by size: 4992 683 56 BtsI GCAGTG_nn' Cuts at: 2520 2639 2812 3247 4041 4061 2520 Size: 119 173 435 794 20 4190 Fragments arranged by size: 4190 794 435 173 119 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DraIII CAC_nnn'GTG Cuts at: 2325 2325 Size: 5731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2396 3178 4067 Size: 54 1425 50 275 18 566 782 889 Cuts at: 4067 5696 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 782 566 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2396 3178 1537 Size: 275 584 782 4090 Fragments arranged by size: 4090 782 584 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 3661 1353 Size: 801 1507 3423 Fragments arranged by size: 3423 1507 801 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4318 4692 4838 387 Size: 1064 138 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2221 2454 2221 Size: 233 5498 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3794 4391 5178 5446 1239 Size: 393 2162 597 787 268 1524 Fragments arranged by size: 2162 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2133 2133 Size: 5731 EcoO109I rG'GnC_Cy Cuts at: 2206 2283 2402 2558 2206 Size: 77 119 156 5379 Fragments arranged by size: 5379 156 119 77 EcoRI G'AATT_C Cuts at: 3040 3418 3040 Size: 378 5353 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 2121 4878 1678 Size: 443 2757 2531 Fragments arranged by size: 2757 2531 443 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4372 4404 4446 4478 1373 Size: 32 135 32 2800 32 42 32 2626 Fragments arranged by size: 2800 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 2698 748 Size: 1950 3781 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KpnI G_GTAC'C Cuts at: 3056 3056 Size: 5731 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2787 4845 5029 1305 Size: 1482 2058 184 2007 Fragments arranged by size: 2058 2007 1482 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3909 Fragments arranged by size: 3909 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2079 3689 4048 4207 518 Size: 648 843 70 1610 359 159 2042 Fragments arranged by size: 2042 1610 843 648 359 159 70 MspA1I CmG'CkG Cuts at: 1606 3023 3147 3824 4972 5217 1606 Size: 1417 124 677 1148 245 2120 Fragments arranged by size: 2120 1417 1148 677 245 124 NcoI C'CATG_G Cuts at: 513 1065 2602 513 Size: 552 1537 3642 Fragments arranged by size: 3642 1537 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2040 3089 4634 1132 Size: 97 811 1049 1545 2229 Fragments arranged by size: 2229 1545 1049 811 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 2325 3023 1108 Size: 1217 698 3816 Fragments arranged by size: 3816 1217 698 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PmlI CAC'GTG Cuts at: 2077 2143 2077 Size: 66 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2490 2522 3800 3832 5313 5345 2490 Size: 32 1278 32 1481 32 2876 Fragments arranged by size: 2876 1481 1278 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2283 2558 2206 Size: 77 275 5379 Fragments arranged by size: 5379 275 77 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PspOMI G'GGCC_C Cuts at: 2401 2401 Size: 5731 PstI C_TGCA'G Cuts at: 830 2444 2515 3083 830 Size: 1614 71 568 3478 Fragments arranged by size: 3478 1614 568 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 3023 3023 Size: 5731 SacI G_AGCT'C Cuts at: 2223 2456 2223 Size: 233 5498 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SanDI GG'GwC_CC Cuts at: 2206 2206 Size: 5731 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 2287 2440 2511 2581 3079 4214 4895 Size: 219 1242 153 71 70 498 1135 681 Cuts at: 4895 5086 826 Size: 191 1471 Fragments arranged by size: 1471 1242 1135 681 498 219 191 153 71 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 3058 1872 Size: 1186 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2130 3835 4736 4998 820 Size: 197 926 187 1705 901 262 1553 Fragments arranged by size: 1705 1553 926 901 262 197 187 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 3089 1132 Size: 1957 3774 SspI AAT'ATT Cuts at: 5 52 2235 3655 5 Size: 47 2183 1420 2081 Fragments arranged by size: 2183 2081 1420 47 StuI AGG'CCT Cuts at: 1517 2951 1517 Size: 1434 4297 StyI C'CwwG_G Cuts at: 513 1065 2602 3170 513 Size: 552 1537 568 3074 Fragments arranged by size: 3074 1537 568 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 2069 2350 2582 2701 3758 3775 Size: 1255 452 37 281 232 119 1057 17 Cuts at: 3775 3928 4113 4558 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1057 452 445 281 232 185 153 119 37 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 3977 5689 Size: 274 80 33 51 1443 213 1787 1712 Cuts at: 5689 96 Size: 138 Fragments arranged by size: 1787 1712 1443 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2297 2329 2412 2444 5691 5723 2127 Size: 32 138 32 83 32 3247 32 2135 Fragments arranged by size: 3247 2135 138 83 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2434 1316 Size: 1118 4613 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2344 2344 Size: 5731 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 3056 1870 Size: 1186 4545 XmnI GAAnn'nnTTC Cuts at: 2246 2665 3860 2246 Size: 419 1195 4117 Fragments arranged by size: 4117 1195 419 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI