Restriction map for
pFN21AB8659
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BcgI BfrI BglI BglII
BlnI BsaAI BsaWI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrGI BssSI
BstEII BstPI BstXI Bsu36I ClaI
CpoI DraI DrdI DsaI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII HpaI KpnI KspI MluI
MluNI MroI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SfiI
SgrAI SmlI SnaBI SphI Sse8387I
SspBI StuI SwaI Van91I XhoI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 419 GACGTC GACGT^C
AccII 1595 CGCG CG^CG
AflIII 1440 ACATGT A^C[GA][CT]GT
ApaI 41 GGGCCC GGGCC^C
AspI 1612 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1849 CCCGGG C^[CT]CG[GA]G
BanI 491 GGCGCC G^G[CT][GA]CC
BbeI 491 GGCGCC GGCGC^C
BbvCI 253 GCTGAGG CCTCAGC(-5/-2)
BlpI 655 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 655 GCTGAGC GC^T[ACGT]AGC
BsaBI 1489 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 345 GAGACC GGTCTC(1/5)
BsmBI 417 GAGACG CGTCTC(1/5)
BsoBI 1849 CCCGGG C^[CT]CG[GA]G
BspHI 1556 TCATGA T^CATGA
BspLU11I 1440 ACATGT A^CATGT
BsrFI 22 GCCGGT [GA]^CCGG[CT]
BssHII 1594 GCGCGC G^CGCGC
Bst1107I 335 GTATAC GTA^TAC
BstBI 685 TTCGAA TT^CGAA
BstUI 1595 CGCG CG^CG
BstZ17I 335 GTATAC GTA^TAC
CelII 655 GCTGAGC GC^T[ACGT]AGC
Cfr10I 22 GCCGGT [GA]^CCGG[CT]
DraII 41 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 878 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 20 CGGCCG C^GGCCG
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
EcoO109I 41 GGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 1897 GCGGG CCCGC(4/6)
HaeII 491 GGCGCC [GA]GCGC^[CT]
KasI 491 GGCGCC G^GCGCC
MamI 1489 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1379 CAATTG C^AATTG
MunI 1379 CAATTG C^AATTG
MvnI 1595 CGCG CG^CG
NarI 491 GGCGCC GG^CGCC
NspV 685 TTCGAA TT^CGAA
PflFI 1612 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 269 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1556 TCATGA T^CATGA
SexAI 1251 ACCTGGT A^CC[AT]GGT
SfoI 491 GGCGCC GGC^GCC
SfuI 685 TTCGAA TT^CGAA
SmaI 1849 CCCGGG CCC^GGG
SpeI 1416 ACTAGT A^CTAGT
Tth111I 1612 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 408 TCTAGA T^CTAGA
XcmI 1641 CCATCCTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1849 CCCGGG C^CCGGG
XmaI 1849 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 477 AAATTT [GA]^AATT[CT]
830 AAATTT
AcyI 419 GACGTC G[GA]^CG[CT]C
491 GGCGCC
AfaI 681 GTAC GT^AC
1635 GTAC
AlwI 1495 GATCC GGATC(4/5)
1660 GATCC
ApoI 477 AAATTT [GA]^AATT[CT]
830 AAATTT
AvaII 531 GGTCC G^G[AT]CC
976 GGTCC
BbsI 943 GAAGAC GAAGAC(2/6)
970 GAAGAC
BciVI 1540 GGATAC GTATCC(6/5)
1090 GTATCC
BclI 629 TGATCA T^GATCA
1383 TGATCA
BpmI 501 CTGGAG CTGGAG(16/14)
1108 CTGGAG
BpuAI 943 GAAGAC GAAGAC(2/6)
970 GAAGAC
BsaHI 419 GACGTC G[GA]^CG[CT]C
491 GGCGCC
BsiEI 20 CGGCCG CG[GA][CT]^CG
1163 CGGTCG
BsmAI 345 GAGAC GTCTC(1/5)
417 GAGAC
BsmFI 36 GGGAC GGGAC(10/14)
977 GTCCC
BstAPI 196 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1870 GCATCCACTGC
BstYI 1494 AGATCC [GA]^GATC[CT]
1659 AGATCC
EaeI 20 CGGCCG [CT]^GGCC[GA]
725 TGGCCG
EcoT14I 980 CCAAGG C^C[AT][AT]GG
1346 CCAAGG
FbaI 629 TGATCA T^GATCA
1383 TGATCA
Hin1I 419 GACGTC G[GA]^CG[CT]C
491 GGCGCC
HincII 1527 GTTGAC GT[CT]^[GA]AC
1774 GTTGAC
HindII 1527 GTTGAC GT[CT]^[GA]AC
1774 GTTGAC
MflI 1494 AGATCC [GA]^GATC[CT]
1659 AGATCC
MslI 103 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1212 CACCATCATG
NspI 1440 ACATGT [GA]CATG^[CT]
1544 ACATGC
RsaI 681 GTAC GT^AC
1635 GTAC
SspI 235 AATATT AAT^ATT
1429 AATATT
StyI 980 CCAAGG C^C[AT][AT]GG
1346 CCAAGG
XhoII 1494 AGATCC [GA]^GATC[CT]
1659 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccI AciI AluI AlwNI AspHI
BanII BbvI BcnI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I