(Circular) (Six-base) MAPSORT of: pFN21AB8659.seq Check: 5111 from: 1 to: 6679 pFN21AB8659 6679 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2428 4489 278 Size: 53 83 186 936 892 2061 2468 Fragments arranged by size: 2468 2061 936 892 186 83 53 AccI GT'mk_AC Cuts at: 1937 2341 3347 3955 4022 1937 Size: 404 1006 608 67 4594 Fragments arranged by size: 4594 1006 608 404 67 Acc65I G'GTAC_C Cuts at: 4000 4000 Size: 6679 AclI AA'CG_TT Cuts at: 1563 4806 5179 1563 Size: 3243 373 3063 Fragments arranged by size: 3243 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 3632 4742 6126 6394 1632 Size: 506 1494 1110 1384 268 1917 Fragments arranged by size: 1917 1494 1384 1110 506 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6482 AflIII A'CryG_T Cuts at: 1203 3445 5578 6555 1203 Size: 2242 2133 977 1327 Fragments arranged by size: 2242 2133 1327 977 AgeI A'CCGG_T Cuts at: 5510 5510 Size: 6679 AhdI GACnn_n'nnGTC Cuts at: 5408 5408 Size: 6679 AleI CACnn'nnGTG Cuts at: 1166 2112 1166 Size: 946 5733 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2688 1184 Size: 1504 5175 AlwNI CAG_nnn'CTG Cuts at: 2165 2458 3035 4422 5994 2165 Size: 293 577 1387 1572 2850 Fragments arranged by size: 2850 1572 1387 577 293 ApaI G_GGCC'C Cuts at: 2050 2050 Size: 6679 ApaLI G'TGCA_C Cuts at: 4736 5892 4736 Size: 1156 5523 ApoI r'AATT_y Cuts at: 1493 2482 2835 3988 4214 4366 6475 1493 Size: 989 353 1153 226 152 2109 1697 Fragments arranged by size: 2109 1697 1153 989 353 226 152 AseI AT'TA_AT Cuts at: 160 1032 5233 160 Size: 872 4201 1606 Fragments arranged by size: 4201 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6679 AvaI C'yCGr_G Cuts at: 1870 1943 3854 4004 1870 Size: 73 1911 150 4545 Fragments arranged by size: 4545 1911 150 73 BamHI G'GATC_C Cuts at: 4009 4376 4009 Size: 367 6312 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2496 4000 5455 618 Size: 325 217 627 709 1504 1455 1842 Fragments arranged by size: 1842 1504 1455 709 627 325 217 BanII G_rGCy'C Cuts at: 1391 2023 2050 2177 3120 3890 3998 1391 Size: 632 27 127 943 770 108 4072 Fragments arranged by size: 4072 943 770 632 127 108 27 BbeI G_GCGC'C Cuts at: 2500 2500 Size: 6679 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2955 2982 928 Size: 971 1056 27 4625 Fragments arranged by size: 4625 1056 971 27 BbvCI CC'TCA_GC Cuts at: 2259 2259 Size: 6679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4870 4904 1903 Size: 34 2933 34 3678 Fragments arranged by size: 3678 2933 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3106 3539 4571 5781 3106 Size: 433 1032 1210 4004 Fragments arranged by size: 4004 1210 1032 433 BclI T'GATC_A Cuts at: 1548 2634 3388 1548 Size: 1086 754 4839 Fragments arranged by size: 4839 1086 754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5290 136 Size: 107 122 71 631 605 3618 1525 Fragments arranged by size: 3618 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6674 6674 Size: 6679 BlpI GC'TnA_GC Cuts at: 2661 4096 2661 Size: 1435 5244 Bme1580I G_kGCm'C Cuts at: 1790 2050 4740 5896 1790 Size: 260 2690 1156 2573 Fragments arranged by size: 2690 2573 1156 260 BmgBI CAC'GTC Cuts at: 1318 2964 1318 Size: 1646 5033 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3147 4410 5368 Size: 416 53 83 399 24 1730 1263 958 Cuts at: 5368 442 Size: 1753 Fragments arranged by size: 1753 1730 1263 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6679 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2527 3134 5339 1239 Size: 1288 607 2205 2579 Fragments arranged by size: 2579 2205 1288 607 Bpu10I CC'TnA_GC Cuts at: 2259 2259 Size: 6679 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4804 5669 5967 4804 Size: 865 298 5516 Fragments arranged by size: 5516 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2344 5342 882 Size: 617 845 2998 2219 Fragments arranged by size: 2998 2219 845 617 BsaAI yAC'GTr Cuts at: 493 1204 4470 493 Size: 711 3266 2702 Fragments arranged by size: 3266 2702 711 BsaBI GATnn'nnATC Cuts at: 3498 6673 3498 Size: 3175 3504 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2425 2497 4486 4868 Size: 53 83 186 936 892 72 1989 382 Cuts at: 4868 5503 275 Size: 635 1451 Fragments arranged by size: 1989 1451 936 892 635 382 186 83 72 53 BsaWI w'CCGG_w Cuts at: 5112 5510 5784 5931 6342 5112 Size: 398 274 147 411 5449 Fragments arranged by size: 5449 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 1178 Size: 30 165 30 678 30 5746 Fragments arranged by size: 5746 678 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2303 2362 2954 2957 3642 1186 Size: 843 274 59 592 3 685 4223 Fragments arranged by size: 4223 843 685 592 274 59 3 BseYI C'CCAG_C Cuts at: 2038 2863 5882 6378 2038 Size: 825 3019 496 2339 Fragments arranged by size: 3019 2339 825 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2028 3171 4129 4890 5918 1540 Size: 275 186 27 1143 958 761 1028 2301 Fragments arranged by size: 2301 1143 1028 958 761 275 186 27 BsiHKAI G_wGCw'C Cuts at: 2177 2969 3120 3890 4740 4825 5896 2177 Size: 792 151 770 850 85 1071 2960 Fragments arranged by size: 2960 1071 850 792 770 151 85 BsmI GAATG_Cn' Cuts at: 4190 4283 4190 Size: 93 6586 BsmBI CGTCTCn'nnnn_ Cuts at: 2416 2416 Size: 6679 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2050 2177 2969 3120 3890 3998 Size: 399 233 27 127 792 151 770 108 Cuts at: 3998 4740 4825 5896 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 792 770 742 399 233 151 127 108 85 27 BspEI T'CCGG_A Cuts at: 6342 6342 Size: 6679 BspHI T'CATG_A Cuts at: 3561 4568 6298 3561 Size: 1007 1730 3942 Fragments arranged by size: 3942 1730 1007 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4034 844 Size: 3190 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4566 1394 Size: 429 2743 3507 Fragments arranged by size: 3507 2743 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5174 5348 65 Size: 1183 3926 174 1396 Fragments arranged by size: 3926 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 5323 5510 1700 Size: 110 217 3296 187 2869 Fragments arranged by size: 3296 2869 217 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6679 BssHII G'CGCG_C Cuts at: 1925 3599 1925 Size: 1674 5005 BssSI C'ACGA_G Cuts at: 4739 5751 4739 Size: 1012 5667 BstAPI GCAn_nnn'nTGC Cuts at: 794 2207 3881 794 Size: 1413 1674 3592 Fragments arranged by size: 3592 1674 1413 BstBI TT'CG_AA Cuts at: 2691 5483 2691 Size: 2792 3887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6679 BstYI r'GATC_y Cuts at: 1972 3499 3664 4009 4376 4761 4778 6230 6316 Size: 1527 165 345 367 385 17 1452 86 Cuts at: 6316 6328 6674 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1527 1452 385 367 346 345 165 86 17 12 BstZ17I GTA'TAC Cuts at: 2342 2342 Size: 6679 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5188 Fragments arranged by size: 5188 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5940 Fragments arranged by size: 5940 683 56 BtsI GCAGTG_nn' Cuts at: 3879 4195 4989 5009 3879 Size: 316 794 20 5549 Fragments arranged by size: 5549 794 316 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6679 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6644 DraI TTT'AAA Cuts at: 3983 3983 Size: 6679 DraIII CAC_nnn'GTG Cuts at: 2888 2888 Size: 6679 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5686 809 Size: 507 4370 1802 Fragments arranged by size: 4370 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2730 4126 Size: 54 1425 50 275 18 195 705 1396 Cuts at: 4126 5015 6644 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1396 889 705 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2025 4126 1537 Size: 275 213 2101 4090 Fragments arranged by size: 4090 2101 275 213 EarI CTCTTCn'nnn_ Cuts at: 1353 2439 2760 2985 3059 3655 4609 1353 Size: 1086 321 225 74 596 954 3423 Fragments arranged by size: 3423 1086 954 596 321 225 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5266 5640 5786 387 Size: 1064 138 3677 374 146 1280 Fragments arranged by size: 3677 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2175 3118 3888 2175 Size: 943 770 4966 Fragments arranged by size: 4966 943 770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2527 3134 3632 4742 5339 6126 Size: 393 506 389 607 498 1110 597 787 Cuts at: 6126 6394 1239 Size: 268 1524 Fragments arranged by size: 1524 1110 787 607 597 506 498 393 389 268 EcoO109I rG'GnC_Cy Cuts at: 2047 2047 Size: 6679 EcoRI G'AATT_C Cuts at: 3988 4366 3988 Size: 378 6301 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2665 2665 Size: 6679 FspI TGC'GCA Cuts at: 5185 5185 Size: 6679 HaeII r_GCGC'y Cuts at: 1678 2500 5826 1678 Size: 822 3326 2531 Fragments arranged by size: 3326 2531 822 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2452 2484 3385 3417 5320 Size: 32 135 32 880 32 901 32 1903 Cuts at: 5320 5352 5394 5426 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1903 901 880 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3781 4023 4269 669 Size: 988 281 1596 247 242 246 3079 Fragments arranged by size: 3079 1596 988 281 247 246 242 HindIII A'AGCT_T Cuts at: 748 748 Size: 6679 HpaI GTT'AAC Cuts at: 4269 4269 Size: 6679 KasI G'GCGC_C Cuts at: 2496 2496 Size: 6679 KpnI G_GTAC'C Cuts at: 4004 4004 Size: 6679 MfeI C'AATT_G Cuts at: 3384 4278 3384 Size: 894 5785 MluI A'CGCG_T Cuts at: 6555 6555 Size: 6679 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3151 3539 5793 5977 1305 Size: 1846 388 2254 184 2007 Fragments arranged by size: 2254 2007 1846 388 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4857 Fragments arranged by size: 4857 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 3221 4637 4996 5155 518 Size: 648 946 1109 1416 359 159 2042 Fragments arranged by size: 2042 1416 1109 946 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2668 3372 4095 4772 5920 6165 1606 Size: 465 597 704 723 677 1148 245 2120 Fragments arranged by size: 2120 1148 723 704 677 597 465 245 NarI GG'CG_CC Cuts at: 2497 2497 Size: 6679 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5188 Fragments arranged by size: 5188 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6679 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6679 NotI GC'GGCC_GC Cuts at: 4126 4126 Size: 6679 NspI r_CATG'y Cuts at: 1132 1229 3449 3553 4037 5582 1132 Size: 97 2220 104 484 1545 2229 Fragments arranged by size: 2229 2220 1545 484 104 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6679 PciI A'CATG_T Cuts at: 3445 5578 3445 Size: 2133 4546 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6679 PfoI T'CCnGG_A Cuts at: 3655 3655 Size: 6679 PmeI GTTT'AAAC Cuts at: 3983 3983 Size: 6679 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3390 3422 4748 4780 6261 6293 3390 Size: 32 1326 32 1481 32 3776 Fragments arranged by size: 3776 1481 1326 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2278 1613 Size: 665 6014 PsiI TTA'TAA Cuts at: 4249 4249 Size: 6679 PspOMI G'GGCC_C Cuts at: 2046 2046 Size: 6679 PstI C_TGCA'G Cuts at: 830 2203 3002 3031 4031 830 Size: 1373 799 29 1000 3478 Fragments arranged by size: 3478 1373 1000 799 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6679 SacI G_AGCT'C Cuts at: 2177 3120 3890 2177 Size: 943 770 4966 Fragments arranged by size: 4966 943 770 SalI G'TCGA_C Cuts at: 1936 4021 1936 Size: 2085 4594 SbfI CC_TGCA'GG Cuts at: 4031 4031 Size: 6679 ScaI AGT'ACT Cuts at: 4927 6639 4927 Size: 1712 4967 SexAI A'CCwGG_T Cuts at: 3256 3256 Size: 6679 SfcI C'TryA_G Cuts at: 826 1045 2199 2998 3027 4027 5162 5843 6034 Size: 219 1154 799 29 1000 1135 681 191 Cuts at: 6034 826 Size: 1471 Fragments arranged by size: 1471 1154 1135 1000 799 681 219 191 29 SfoI GGC'GCC Cuts at: 2498 2498 Size: 6679 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6679 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6679 SmaI CCC'GGG Cuts at: 1872 3856 4006 1872 Size: 1984 150 4545 Fragments arranged by size: 4545 1984 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 4783 5684 5946 820 Size: 197 926 2840 901 262 1553 Fragments arranged by size: 2840 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4470 493 Size: 3977 2702 SpeI A'CTAG_T Cuts at: 152 3421 152 Size: 3269 3410 SphI G_CATG'C Cuts at: 1132 4037 1132 Size: 2905 3774 SspI AAT'ATT Cuts at: 5 52 2242 3436 4603 5 Size: 47 2190 1194 1167 2081 Fragments arranged by size: 2190 2081 1194 1167 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6679 StyI C'CwwG_G Cuts at: 513 1065 2004 2985 3351 4118 513 Size: 552 939 981 366 767 3074 Fragments arranged by size: 3074 981 939 767 552 366 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4706 4723 4876 5061 5506 325 Size: 1255 3126 17 153 185 445 1498 Fragments arranged by size: 3126 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2685 3639 4925 Size: 274 80 33 51 1443 708 954 1286 Cuts at: 4925 6637 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1286 954 708 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2081 2113 2834 2866 6639 6671 2081 Size: 32 721 32 3773 32 2089 Fragments arranged by size: 3773 2089 721 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3620 1316 Size: 2304 4375 XbaI T'CTAG_A Cuts at: 2413 4015 2413 Size: 1602 5077 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3653 3653 Size: 6679 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6679 XmaI C'CCGG_G Cuts at: 1870 3854 4004 1870 Size: 1984 150 4545 Fragments arranged by size: 4545 1984 150 XmnI GAAnn'nnTTC Cuts at: 4808 4808 Size: 6679 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2426 4487 276 Size: 53 83 186 936 892 2061 2468 Fragments arranged by size: 2468 2061 936 892 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI