Restriction map for
pFN21AB8659
(Linear) (Six-base) MAPSORT of: KIBB8659.seq Check: 2009 from: 1 to: 1974
KIBB8659.orf
With 182 enzymes: SgfI *
July 28, 2009 13:24 ..
AatII G_ACGT'C
Cuts at: 0 423 1974
Size: 423 1551
AccI GT'mk_AC
Cuts at: 0 336 1342 1950 1974
Size: 336 1006 608 24
Fragments arranged by size:
1006 608 336 24
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1627 1974
Size: 133 1494 347
Fragments arranged by size:
1494 347 133
AflIII A'CryG_T
Cuts at: 0 1440 1974
Size: 1440 534
AleI CACnn'nnGTG
Cuts at: 0 107 1974
Size: 107 1867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 683 1974
Size: 683 1291
AlwNI CAG_nnn'CTG
Cuts at: 0 160 453 1030 1974
Size: 160 293 577 944
Fragments arranged by size:
944 577 293 160
ApaI G_GGCC'C
Cuts at: 0 45 1974
Size: 45 1929
ApoI r'AATT_y
Cuts at: 0 477 830 1974
Size: 477 353 1144
Fragments arranged by size:
1144 477 353
AvaI C'yCGr_G
Cuts at: 0 1849 1974
Size: 1849 125
BanI G'GyrC_C
Cuts at: 0 491 1974
Size: 491 1483
BanII G_rGCy'C
Cuts at: 0 18 45 172 1115 1885 1974
Size: 18 27 127 943 770 89
Fragments arranged by size:
943 770 127 89 27 18
BbeI G_GCGC'C
Cuts at: 0 495 1974
Size: 495 1479
BbsI GAAGACnn'nnnn_
Cuts at: 0 950 977 1974
Size: 950 27 997
Fragments arranged by size:
997 950 27
BbvCI CC'TCA_GC
Cuts at: 0 254 1974
Size: 254 1720
BciVI GTATCCnnnnn_n'
Cuts at: 0 1101 1534 1974
Size: 1101 433 440
Fragments arranged by size:
1101 440 433
BclI T'GATC_A
Cuts at: 0 629 1383 1974
Size: 629 754 591
Fragments arranged by size:
754 629 591
BlpI GC'TnA_GC
Cuts at: 0 656 1974
Size: 656 1318
Bme1580I G_kGCm'C
Cuts at: 0 45 1974
Size: 45 1929
BmgBI CAC'GTC
Cuts at: 0 959 1974
Size: 959 1015
BmrI ACTGGGnnnn_n'
Cuts at: 0 1142 1974
Size: 1142 832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1129 1974
Size: 522 607 845
Fragments arranged by size:
845 607 522
Bpu10I CC'TnA_GC
Cuts at: 0 254 1974
Size: 254 1720
BsaI GGTCTCn'nnnn_
Cuts at: 0 339 1974
Size: 339 1635
BsaBI GATnn'nnATC
Cuts at: 0 1493 1974
Size: 1493 481
BsaHI Gr'CG_yC
Cuts at: 0 420 492 1974
Size: 420 72 1482
Fragments arranged by size:
1482 420 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 1974
Size: 76 30 1868
Fragments arranged by size:
1868 76 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 298 357 949 952 1637 1974
Size: 24 274 59 592 3 685 337
Fragments arranged by size:
685 592 337 274 59 24 3
BseYI C'CCAG_C
Cuts at: 0 33 858 1974
Size: 33 825 1116
Fragments arranged by size:
1116 825 33
BsiEI CG_ry'CG
Cuts at: 0 23 1166 1974
Size: 23 1143 808
Fragments arranged by size:
1143 808 23
BsiHKAI G_wGCw'C
Cuts at: 0 172 964 1115 1885 1974
Size: 172 792 151 770 89
Fragments arranged by size:
792 770 172 151 89
BsmBI CGTCTCn'nnnn_
Cuts at: 0 411 1974
Size: 411 1563
Bsp1286I G_dGCh'C
Cuts at: 0 18 45 172 964 1115 1885 1974
Size: 18 27 127 792 151 770 89
Fragments arranged by size:
792 770 151 127 89 27 18
BspHI T'CATG_A
Cuts at: 0 1556 1974
Size: 1556 418
BsrFI r'CCGG_y
Cuts at: 0 22 1974
Size: 22 1952
BssHII G'CGCG_C
Cuts at: 0 1594 1974
Size: 1594 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 202 1876 1974
Size: 202 1674 98
Fragments arranged by size:
1674 202 98
BstBI TT'CG_AA
Cuts at: 0 686 1974
Size: 686 1288
BstYI r'GATC_y
Cuts at: 0 1494 1659 1974
Size: 1494 165 315
Fragments arranged by size:
1494 315 165
BstZ17I GTA'TAC
Cuts at: 0 337 1974
Size: 337 1637
BtsI GCAGTG_nn'
Cuts at: 0 1874 1974
Size: 1874 100
DraIII CAC_nnn'GTG
Cuts at: 0 883 1974
Size: 883 1091
EaeI y'GGCC_r
Cuts at: 0 20 725 1974
Size: 20 705 1249
Fragments arranged by size:
1249 705 20
EagI C'GGCC_G
Cuts at: 0 20 1974
Size: 20 1954
EarI CTCTTCn'nnn_
Cuts at: 0 434 755 980 1054 1650 1974
Size: 434 321 225 74 596 324
Fragments arranged by size:
596 434 324 321 225 74
EcoICRI GAG'CTC
Cuts at: 0 170 1113 1883 1974
Size: 170 943 770 91
Fragments arranged by size:
943 770 170 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 522 1129 1627 1974
Size: 133 389 607 498 347
Fragments arranged by size:
607 498 389 347 133
EcoO109I rG'GnC_Cy
Cuts at: 0 42 1974
Size: 42 1932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 660 1974
Size: 660 1314
HaeII r_GCGC'y
Cuts at: 0 495 1974
Size: 495 1479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 447 479 1380 1412 1974
Size: 447 32 901 32 562
Fragments arranged by size:
901 562 447 32 32
HincII GTy'rAC
Cuts at: 0 1529 1776 1974
Size: 1529 247 198
Fragments arranged by size:
1529 247 198
KasI G'GCGC_C
Cuts at: 0 491 1974
Size: 491 1483
MfeI C'AATT_G
Cuts at: 0 1379 1974
Size: 1379 595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1146 1534 1974
Size: 1146 388 440
Fragments arranged by size:
1146 440 388
MslI CAynn'nnrTG
Cuts at: 0 107 1216 1974
Size: 107 1109 758
Fragments arranged by size:
1109 758 107
MspA1I CmG'CkG
Cuts at: 0 66 663 1367 1974
Size: 66 597 704 607
Fragments arranged by size:
704 607 597 66
NarI GG'CG_CC
Cuts at: 0 492 1974
Size: 492 1482
NspI r_CATG'y
Cuts at: 0 1444 1548 1974
Size: 1444 104 426
Fragments arranged by size:
1444 426 104
PciI A'CATG_T
Cuts at: 0 1440 1974
Size: 1440 534
PfoI T'CCnGG_A
Cuts at: 0 1650 1974
Size: 1650 324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1385 1417 1974
Size: 1385 32 557
Fragments arranged by size:
1385 557 32
PshAI GACnn'nnGTC
Cuts at: 0 273 1974
Size: 273 1701
PspOMI G'GGCC_C
Cuts at: 0 41 1974
Size: 41 1933
PstI C_TGCA'G
Cuts at: 0 198 997 1026 1974
Size: 198 799 29 948
Fragments arranged by size:
948 799 198 29
SacI G_AGCT'C
Cuts at: 0 172 1115 1885 1974
Size: 172 943 770 89
Fragments arranged by size:
943 770 172 89
SexAI A'CCwGG_T
Cuts at: 0 1251 1974
Size: 1251 723
SfcI C'TryA_G
Cuts at: 0 194 993 1022 1974
Size: 194 799 29 952
Fragments arranged by size:
952 799 194 29
SfoI GGC'GCC
Cuts at: 0 493 1974
Size: 493 1481
SmaI CCC'GGG
Cuts at: 0 1851 1974
Size: 1851 123
SpeI A'CTAG_T
Cuts at: 0 1416 1974
Size: 1416 558
SspI AAT'ATT
Cuts at: 0 237 1431 1974
Size: 237 1194 543
Fragments arranged by size:
1194 543 237
StyI C'CwwG_G
Cuts at: 0 980 1346 1974
Size: 980 366 628
Fragments arranged by size:
980 628 366
TatI w'GTAC_w
Cuts at: 0 680 1634 1974
Size: 680 954 340
Fragments arranged by size:
954 680 340
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 76 108 829 861 1974
Size: 76 32 721 32 1113
Fragments arranged by size:
1113 721 76 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1615 1974
Size: 1615 359
XbaI T'CTAG_A
Cuts at: 0 408 1974
Size: 408 1566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1648 1974
Size: 1648 326
XmaI C'CCGG_G
Cuts at: 0 1849 1974
Size: 1849 125
ZraI GAC'GTC
Cuts at: 0 421 1974
Size: 421 1553
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BsrFI BssHII BstAPI BstBI BstYI BstZ17I
BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HincII KasI MfeI MmeI MslI
MspA1I NarI NspI PciI PfoI PpiI PshAI PspOMI
PstI SacI SexAI SfcI SfoI SmaI SpeI SspI
StyI TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BfrBI BglI BglII BmtI BplI BpuEI BsaAI BsaWI
BsgI BsiWI BsmI BspEI BspMI BsrBI BsrDI BsrGI
BssSI BstEII BstXI Bsu36I BtgI BtgZI ClaI CspCI
DraI DrdI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MluI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpuMI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmlI SnaBI SphI SrfI StuI
SwaI TaqII XhoI XmnI