(Circular) (Six-base) MAPSORT of: pFN21AE9668.seq Check: 1609 from: 1 to: 8791 pFN21AE9668 8791 bp With 182 enzymes: SgfI * December 21, 2016 11:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4500 4527 4768 5448 4500 Size: 27 241 680 7843 Fragments arranged by size: 7843 680 241 27 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3270 6601 278 Size: 53 83 186 936 1734 3331 2468 Fragments arranged by size: 3331 2468 1734 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2084 6134 1937 Size: 147 4050 4594 Fragments arranged by size: 4594 4050 147 Acc65I G'GTAC_C Cuts at: 2590 6112 2590 Size: 3522 5269 AclI AA'CG_TT Cuts at: 1563 6918 7291 1563 Size: 5355 373 3063 Fragments arranged by size: 5355 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3956 4001 5362 5747 6854 8238 8506 1632 Size: 2324 45 1361 385 1107 1384 268 1917 Fragments arranged by size: 2324 1917 1384 1361 1107 385 268 45 AfeI AGC'GCT Cuts at: 2342 4010 2342 Size: 1668 7123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8594 AflIII A'CryG_T Cuts at: 1203 7690 8667 1203 Size: 6487 977 1327 Fragments arranged by size: 6487 1327 977 AgeI A'CCGG_T Cuts at: 5171 7622 5171 Size: 2451 6340 AhdI GACnn_n'nnGTC Cuts at: 7520 7520 Size: 8791 AleI CACnn'nnGTG Cuts at: 1166 4415 1166 Size: 3249 5542 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5925 1184 Size: 4741 4050 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5588 5620 5588 Size: 32 8759 AlwNI CAG_nnn'CTG Cuts at: 2743 3535 4807 4912 5239 5290 6534 8106 2743 Size: 792 1272 105 327 51 1244 1572 3428 Fragments arranged by size: 3428 1572 1272 1244 792 327 105 51 ApaI G_GGCC'C Cuts at: 5527 5830 5527 Size: 303 8488 ApaLI G'TGCA_C Cuts at: 3566 3905 4994 6848 8004 3566 Size: 339 1089 1854 1156 4353 Fragments arranged by size: 4353 1854 1156 1089 339 ApoI r'AATT_y Cuts at: 1493 3940 6046 6100 6326 6478 8587 1493 Size: 2447 2106 54 226 152 2109 1697 Fragments arranged by size: 2447 2109 2106 1697 226 152 54 AseI AT'TA_AT Cuts at: 160 1032 7345 160 Size: 872 6313 1606 Fragments arranged by size: 6313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8791 AvaI C'yCGr_G Cuts at: 1870 1943 4196 5697 6116 1870 Size: 73 2253 1501 419 4545 Fragments arranged by size: 4545 2253 1501 419 73 BamHI G'GATC_C Cuts at: 6121 6488 6121 Size: 367 8424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2590 2728 4191 4417 5145 Size: 325 217 627 803 138 1463 226 728 Cuts at: 5145 5847 6112 7567 618 Size: 702 265 1455 1842 Fragments arranged by size: 1842 1463 1455 803 728 702 627 325 265 226 217 138 BanII G_rGCy'C Cuts at: 1391 2943 3529 3546 3930 4121 4434 5376 5403 Size: 1552 586 17 384 191 313 942 27 Cuts at: 5403 5527 5606 5830 5948 6110 1391 Size: 124 79 224 118 162 4072 Fragments arranged by size: 4072 1552 942 586 384 313 224 191 162 124 118 79 27 17 BbeI G_GCGC'C Cuts at: 5851 5851 Size: 8791 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2858 4155 5564 6016 928 Size: 971 959 1297 1409 452 3703 Fragments arranged by size: 3703 1409 1297 971 959 452 BbvCI CC'TCA_GC Cuts at: 2637 5112 2637 Size: 2475 6316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3560 3594 6982 7016 1903 Size: 34 1623 34 3388 34 3678 Fragments arranged by size: 3678 3388 1623 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2390 6683 7893 2390 Size: 4293 1210 3288 Fragments arranged by size: 4293 3288 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2176 4618 4660 Size: 107 122 71 631 605 504 2442 42 Cuts at: 4660 5449 5729 5825 7402 136 Size: 789 280 96 1577 1525 Fragments arranged by size: 2442 1577 1525 789 631 605 504 280 122 107 96 71 42 BglII A'GATC_T Cuts at: 8786 8786 Size: 8791 BlpI GC'TnA_GC Cuts at: 3540 3857 6208 3540 Size: 317 2351 6123 Fragments arranged by size: 6123 2351 317 Bme1580I G_kGCm'C Cuts at: 1790 2766 3012 3426 3570 3909 4194 4266 4422 Size: 976 246 414 144 339 285 72 156 Cuts at: 4422 4998 5527 5830 6852 8008 1790 Size: 576 529 303 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 976 576 529 414 339 303 285 246 156 144 72 BmgBI CAC'GTC Cuts at: 1318 2546 3007 3148 5843 1318 Size: 1228 461 141 2695 4266 Fragments arranged by size: 4266 2695 1228 461 141 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3167 5950 6522 Size: 416 53 83 399 24 1750 2783 572 Cuts at: 6522 7480 442 Size: 958 1753 Fragments arranged by size: 2783 1753 1750 958 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8791 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2231 2941 4150 2231 Size: 710 1209 6872 Fragments arranged by size: 6872 1209 710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2451 2775 3245 3326 3530 4025 4105 4448 Size: 1212 324 470 81 204 495 80 343 Cuts at: 4448 4566 4763 5547 5831 7451 1239 Size: 118 197 784 284 1620 2579 Fragments arranged by size: 2579 1620 1212 784 495 470 343 324 284 204 197 118 81 80 Bpu10I CC'TnA_GC Cuts at: 2370 2637 3249 4503 4533 5112 5733 2370 Size: 267 612 1254 30 579 621 5428 Fragments arranged by size: 5428 1254 621 612 579 267 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 2589 3050 3071 4032 6916 7781 8079 2201 Size: 388 461 21 961 2884 865 298 2913 Fragments arranged by size: 2913 2884 961 865 461 388 298 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 3058 3968 4579 7454 882 Size: 617 645 914 910 611 2875 2219 Fragments arranged by size: 2875 2219 914 910 645 617 611 BsaAI yAC'GTr Cuts at: 493 1204 3798 6582 493 Size: 711 2594 2784 2702 Fragments arranged by size: 2784 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 3962 8785 3962 Size: 4823 3968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2750 3267 5848 6013 Size: 53 83 186 936 1217 517 2581 165 Cuts at: 6013 6598 6980 7615 275 Size: 585 382 635 1451 Fragments arranged by size: 2581 1451 1217 936 635 585 517 382 186 165 83 53 BsaWI w'CCGG_w Cuts at: 5171 7224 7622 7896 8043 8454 5171 Size: 2053 398 274 147 411 5508 Fragments arranged by size: 5508 2053 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4686 4716 5588 5618 1178 Size: 30 165 30 3283 30 872 30 4351 Fragments arranged by size: 4351 3283 872 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2607 2640 3404 3472 3530 3533 3699 3857 Size: 1421 33 764 68 58 3 166 158 Cuts at: 3857 4069 4126 4569 5354 5635 5723 5800 5849 Size: 212 57 443 785 281 88 77 49 Cuts at: 5849 1186 Size: 4128 Fragments arranged by size: 4128 1421 785 764 443 281 212 166 158 88 77 68 58 57 49 33 3 BseYI C'CCAG_C Cuts at: 2109 2736 2769 2799 2956 3028 3528 3597 3892 Size: 627 33 30 157 72 500 69 295 Cuts at: 3892 4266 4287 4434 4476 4524 4605 4622 4666 Size: 374 21 147 42 48 81 17 44 Cuts at: 4666 4860 4944 4950 4962 5259 5316 5445 5949 Size: 194 84 6 12 297 57 129 504 Cuts at: 5949 7994 8490 2109 Size: 2045 496 2410 Fragments arranged by size: 2410 2045 627 504 500 496 374 297 295 194 157 147 129 84 81 72 69 57 48 44 42 33 30 21 17 12 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2678 4472 4838 4874 4922 5642 1163 Size: 573 942 1794 366 36 48 720 4312 Fragments arranged by size: 4312 1794 942 720 573 366 48 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5396 5453 6241 7002 8030 1540 Size: 275 186 3395 57 788 761 1028 2301 Fragments arranged by size: 3395 2301 1028 788 761 275 186 57 BsiHKAI G_wGCw'C Cuts at: 2217 3209 3546 3570 3909 4121 4539 4998 5745 Size: 992 337 24 339 212 418 459 747 Cuts at: 5745 5948 6852 6937 8008 2217 Size: 203 904 85 1071 3000 Fragments arranged by size: 3000 1071 992 904 747 459 418 339 337 212 203 85 24 BsmI GAATG_Cn' Cuts at: 2371 6302 6395 2371 Size: 3931 93 4767 Fragments arranged by size: 4767 3931 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2766 2943 3012 3209 3426 3529 Size: 399 427 549 177 69 197 217 103 Cuts at: 3529 3546 3570 3909 3930 4121 4194 4266 4422 Size: 17 24 339 21 191 73 72 156 Cuts at: 4422 4434 4539 4998 5376 5403 5527 5606 5745 Size: 12 105 459 378 27 124 79 139 Cuts at: 5745 5830 5948 6110 6852 6937 8008 1391 Size: 85 118 162 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 549 459 427 399 378 339 217 197 191 177 162 156 139 124 118 105 103 85 85 79 73 72 69 27 24 21 17 12 BspEI T'CCGG_A Cuts at: 8454 8454 Size: 8791 BspHI T'CATG_A Cuts at: 2517 2718 6680 8410 2517 Size: 201 3962 1730 2898 Fragments arranged by size: 3962 2898 1730 201 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 4500 4527 4768 4806 5448 6146 844 Size: 1364 2292 27 241 38 642 698 3489 Fragments arranged by size: 3489 2292 1364 698 642 241 38 27 BsrBI CCG'CTC Cuts at: 1394 1823 2019 2789 5696 5764 6678 1394 Size: 429 196 770 2907 68 914 3507 Fragments arranged by size: 3507 2907 914 770 429 196 68 BsrDI GCAATG_nn' Cuts at: 65 1248 3754 4404 7286 7460 65 Size: 1183 2506 650 2882 174 1396 Fragments arranged by size: 2882 2506 1396 1183 650 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2979 4073 5171 7435 7622 1700 Size: 110 1169 1094 1098 2264 187 2869 Fragments arranged by size: 2869 2264 1169 1098 1094 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8791 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8791 BssSI C'ACGA_G Cuts at: 2915 3569 5324 6851 7863 2915 Size: 654 1755 1527 1012 3843 Fragments arranged by size: 3843 1755 1527 1012 654 BstAPI GCAn_nnn'nTGC Cuts at: 794 2200 4807 4816 4912 5008 794 Size: 1406 2607 9 96 96 4577 Fragments arranged by size: 4577 2607 1406 96 96 9 BstBI TT'CG_AA Cuts at: 3354 7595 3354 Size: 4241 4550 BstEII G'GTnAC_C Cuts at: 1184 5174 1184 Size: 3990 4801 BstXI CCAn_nnnn'nTGG Cuts at: 2956 3442 2956 Size: 486 8305 BstYI r'GATC_y Cuts at: 1972 5346 6121 6488 6873 6890 8342 8428 8440 Size: 3374 775 367 385 17 1452 86 12 Cuts at: 8440 8786 1972 Size: 346 1977 Fragments arranged by size: 3374 1977 1452 775 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3387 3387 Size: 8791 BtgI C'CryG_G Cuts at: 513 1065 2586 3195 3327 4716 4985 5304 6024 Size: 552 1521 609 132 1389 269 319 720 Cuts at: 6024 513 Size: 3280 Fragments arranged by size: 3280 1521 1389 720 609 552 319 269 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2812 4394 487 Size: 683 56 1586 1582 4884 Fragments arranged by size: 4884 1586 1582 683 56 BtsI GCAGTG_nn' Cuts at: 2741 3426 3992 4575 6307 7101 7121 2741 Size: 685 566 583 1732 794 20 4411 Fragments arranged by size: 4411 1732 794 685 583 566 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2884 2919 3421 3456 529 Size: 35 2320 35 502 35 5864 Fragments arranged by size: 5864 2320 502 35 35 35 DraI TTT'AAA Cuts at: 6095 6095 Size: 8791 DraIII CAC_nnn'GTG Cuts at: 3998 4570 3998 Size: 572 8219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2702 5248 7798 809 Size: 507 1386 2546 2550 1802 Fragments arranged by size: 2550 2546 1802 1386 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2832 3324 3402 Size: 54 1425 50 275 18 1002 492 78 Cuts at: 3402 4465 5393 5450 6238 7127 8756 8 Size: 1063 928 57 788 889 1629 43 Fragments arranged by size: 1629 1425 1063 1002 928 889 788 492 275 78 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5393 5450 6238 1537 Size: 275 3581 57 788 4090 Fragments arranged by size: 4090 3581 788 275 57 EarI CTCTTCn'nnn_ Cuts at: 1353 2477 2533 2864 3035 3672 3773 3807 4746 Size: 1124 56 331 171 637 101 34 939 Cuts at: 4746 6721 1353 Size: 1975 3423 Fragments arranged by size: 3423 1975 1124 939 637 331 171 101 56 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5058 5675 7378 7752 7898 387 Size: 1064 138 3469 617 1703 374 146 1280 Fragments arranged by size: 3469 1703 1280 1064 617 374 146 138 EcoICRI GAG'CTC Cuts at: 3544 4119 5946 3544 Size: 575 1827 6389 Fragments arranged by size: 6389 1827 575 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 2775 3245 3326 3530 3956 4001 Size: 393 819 324 470 81 204 426 45 Cuts at: 4001 4025 4105 4448 4566 4763 5362 5547 5747 Size: 24 80 343 118 197 599 185 200 Cuts at: 5747 5831 6854 7451 8238 8506 1239 Size: 84 1023 597 787 268 1524 Fragments arranged by size: 1524 1023 819 787 599 597 470 426 393 343 324 268 204 200 197 185 118 84 81 80 45 24 EcoO109I rG'GnC_Cy Cuts at: 4249 5428 5523 5524 5581 5722 4249 Size: 1179 95 1 57 141 7318 Fragments arranged by size: 7318 1179 141 95 57 1 EcoRI G'AATT_C Cuts at: 3940 6100 6478 3940 Size: 2160 378 6253 Fragments arranged by size: 6253 2160 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2359 2491 6101 2359 Size: 132 3610 5049 Fragments arranged by size: 5049 3610 132 FspI TGC'GCA Cuts at: 7297 7297 Size: 8791 HaeII r_GCGC'y Cuts at: 1678 2344 3773 4012 5851 5875 7938 1678 Size: 666 1429 239 1839 24 2063 2531 Fragments arranged by size: 2531 2063 1839 1429 666 239 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3467 3499 5150 5182 6050 Size: 32 135 32 1895 32 1651 32 868 Cuts at: 6050 6082 7432 7464 7506 7538 1373 Size: 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1895 1651 1350 868 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6135 6381 669 Size: 988 281 4197 246 3079 Fragments arranged by size: 4197 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8791 HpaI GTT'AAC Cuts at: 6381 6381 Size: 8791 KasI G'GCGC_C Cuts at: 5847 5847 Size: 8791 KpnI G_GTAC'C Cuts at: 2594 6116 2594 Size: 3522 5269 MfeI C'AATT_G Cuts at: 6390 6390 Size: 8791 MluI A'CGCG_T Cuts at: 8667 8667 Size: 8791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 3309 3786 3969 4092 5053 5136 5657 Size: 876 1128 477 183 123 961 83 521 Cuts at: 5657 7905 8089 1305 Size: 2248 184 2007 Fragments arranged by size: 2248 2007 1128 961 876 521 477 184 183 123 83 MscI TGG'CCA Cuts at: 10 64 1489 1832 3326 10 Size: 54 1425 343 1494 5475 Fragments arranged by size: 5475 1494 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2723 3200 4415 4778 4784 6041 6749 Size: 648 1557 477 1215 363 6 1257 708 Cuts at: 6749 7108 7267 518 Size: 359 159 2042 Fragments arranged by size: 2042 1557 1257 1215 708 648 477 363 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2965 3078 3532 4637 4792 4807 4912 5449 Size: 1359 113 454 1105 155 15 105 537 Cuts at: 5449 5909 6207 6884 8032 8277 1606 Size: 460 298 677 1148 245 2120 Fragments arranged by size: 2120 1359 1148 1105 677 537 460 454 298 245 155 113 105 15 NaeI GCC'GGC Cuts at: 2981 4075 2981 Size: 1094 7697 NarI GG'CG_CC Cuts at: 5848 5848 Size: 8791 NcoI C'CATG_G Cuts at: 513 1065 3195 3327 4716 6024 513 Size: 552 2130 132 1389 1308 3280 Fragments arranged by size: 3280 2130 1389 1308 552 132 NdeI CA'TA_TG Cuts at: 387 387 Size: 8791 NgoMIV G'CCGG_C Cuts at: 2979 4073 2979 Size: 1094 7697 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8791 NotI GC'GGCC_GC Cuts at: 6238 6238 Size: 8791 NspI r_CATG'y Cuts at: 1132 1229 6149 7694 1132 Size: 97 4920 1545 2229 Fragments arranged by size: 4920 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2024 5047 1348 Size: 676 3023 5092 Fragments arranged by size: 5092 3023 676 PciI A'CATG_T Cuts at: 7690 7690 Size: 8791 PflMI CCAn_nnn'nTGG Cuts at: 1108 3319 3535 1108 Size: 2211 216 6364 Fragments arranged by size: 6364 2211 216 PfoI T'CCnGG_A Cuts at: 2382 3342 3546 4425 2382 Size: 960 204 879 6748 Fragments arranged by size: 6748 960 879 204 PmeI GTTT'AAAC Cuts at: 6095 6095 Size: 8791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5588 5620 6860 6892 8373 8405 5588 Size: 32 1240 32 1481 32 5974 Fragments arranged by size: 5974 1481 1240 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5581 5581 Size: 8791 PshAI GACnn'nnGTC Cuts at: 1613 2714 1613 Size: 1101 7690 PsiI TTA'TAA Cuts at: 6361 6361 Size: 8791 PspOMI G'GGCC_C Cuts at: 5523 5826 5523 Size: 303 8488 PstI C_TGCA'G Cuts at: 830 2748 3077 4803 4812 4917 6143 830 Size: 1918 329 1726 9 105 1226 3478 Fragments arranged by size: 3478 1918 1726 1226 329 105 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8791 PvuII CAG'CTG Cuts at: 4792 4807 4912 5909 4792 Size: 15 105 997 7674 Fragments arranged by size: 7674 997 105 15 SacI G_AGCT'C Cuts at: 3546 4121 5948 3546 Size: 575 1827 6389 Fragments arranged by size: 6389 1827 575 SalI G'TCGA_C Cuts at: 1936 6133 1936 Size: 4197 4594 SapI GCTCTTCn'nnn_ Cuts at: 2477 3035 3773 4746 2477 Size: 558 738 973 6522 Fragments arranged by size: 6522 973 738 558 SbfI CC_TGCA'GG Cuts at: 6143 6143 Size: 8791 ScaI AGT'ACT Cuts at: 7039 8751 7039 Size: 1712 7079 SfcI C'TryA_G Cuts at: 826 1045 2744 3073 4799 4808 4913 6139 7274 Size: 219 1699 329 1726 9 105 1226 1135 Cuts at: 7274 7955 8146 826 Size: 681 191 1471 Fragments arranged by size: 1726 1699 1471 1226 1135 681 329 219 191 105 9 SfiI GGCCn_nnn'nGGCC Cuts at: 5825 5825 Size: 8791 SfoI GGC'GCC Cuts at: 5849 5849 Size: 8791 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8791 SmaI CCC'GGG Cuts at: 1872 4198 6118 1872 Size: 2326 1920 4545 Fragments arranged by size: 4545 2326 1920 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 2604 3065 3086 4047 6895 Size: 197 926 273 388 461 21 961 2848 Cuts at: 6895 7796 8058 820 Size: 901 262 1553 Fragments arranged by size: 2848 1553 961 926 901 461 388 273 262 197 21 SnaBI TAC'GTA Cuts at: 493 6582 493 Size: 6089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8791 SphI G_CATG'C Cuts at: 1132 6149 1132 Size: 5017 3774 SspI AAT'ATT Cuts at: 5 52 6072 6715 5 Size: 47 6020 643 2081 Fragments arranged by size: 6020 2081 643 47 StuI AGG'CCT Cuts at: 1517 5482 1517 Size: 3965 4826 StyI C'CwwG_G Cuts at: 513 1065 3195 3327 3435 3699 4716 6024 6230 Size: 552 2130 132 108 264 1017 1308 206 Cuts at: 6230 513 Size: 3074 Fragments arranged by size: 3074 2130 1308 1017 552 264 206 132 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2832 2836 3042 4427 4490 5036 5371 Size: 1255 1252 4 206 1385 63 546 335 Cuts at: 5371 6818 6835 6988 7173 7618 325 Size: 1447 17 153 185 445 1498 Fragments arranged by size: 1498 1447 1385 1255 1252 546 445 335 206 185 153 63 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3297 7037 8749 Size: 274 80 33 51 1443 1320 3740 1712 Cuts at: 8749 96 Size: 138 Fragments arranged by size: 3740 1712 1443 1320 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4686 4718 5346 5378 8751 8783 4686 Size: 32 628 32 3373 32 4694 Fragments arranged by size: 4694 3373 628 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2441 1316 Size: 1125 7666 XbaI T'CTAG_A Cuts at: 6127 6127 Size: 8791 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3439 3439 Size: 8791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8791 XmaI C'CCGG_G Cuts at: 1870 4196 6116 1870 Size: 2326 1920 4545 Fragments arranged by size: 4545 2326 1920 XmnI GAAnn'nnTTC Cuts at: 4094 6920 4094 Size: 2826 5965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3268 6599 276 Size: 53 83 186 936 1734 3331 2468 Fragments arranged by size: 3331 2468 1734 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI