################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I TspRI Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcyI 420 GACGCC G[GA]^CG[CT]C ApaI 521 GGGCCC GGGCC^C AspHI 304 GAGCAC G[AT]GC[AT]^C AspI 254 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 49 GAAGAC GAAGAC(2/6) BbvCI 402 CCTCAGC CCTCAGC(-5/-2) BfaI 349 CTAG C^TAG BlpI 549 GCTGAGC GC^T[ACGT]AGC Bpu1102I 549 GCTGAGC GC^T[ACGT]AGC BpuAI 49 GAAGAC GAAGAC(2/6) BsaHI 420 GACGCC G[GA]^CG[CT]C BsgI 565 GTGCAG GTGCAG(16/14) BsiHKAI 304 GAGCAC G[AT]GC[AT]^C BspMI 116 ACCTGC ACCTGC(4/8) BsrI 648 CCAGT ACTGG(1/-1) BssHII 448 GCGCGC G^CGCGC BstF5I 87 GGATG GGATG(2/0) CelII 549 GCTGAGC GC^T[ACGT]AGC EarI 22 CTCTTC CTCTTC(1/4) EcoT14I 557 CCAAGG C^C[AT][AT]GG FokI 87 GGATG GGATG(9/13) Hin1I 420 GACGCC G[GA]^CG[CT]C HinfI 254 GACTC G^A[ACGT]TC HphI 329 TCACC GGTGA(8/7) Ksp632I 22 CTCTTC CTCTTC(1/4) MaeI 349 CTAG C^TAG MaeII 326 ACGT A^CGT MslI 339 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 315 CATG CATG^ NspI 314 GCATGT [GA]CATG^[CT] PflFI 254 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 254 GACTC GAGTC(4/5) PstI 514 CTGCAG CTGCA^G SfcI 514 CTGCAG C^T[GA][CT]AG StyI 557 CCAAGG C^C[AT][AT]GG TaqI 302 TCGA T^CGA Tsp45I 328 GTCAC ^GT[GC]AC Tsp509I 416 AATT ^AATT Tth111I 254 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 302 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaI 148 CCCGGG C^[CT]CG[GA]G 355 CCCGGG BsoBI 148 CCCGGG C^[CT]CG[GA]G 355 CCCGGG BsrBI 370 CCGCTC CCGCTC(-3/-3) 458 CCGCTC BsrFI 331 ACCGGC [GA]^CCGG[CT] 602 GCCGGT Cfr10I 331 ACCGGC [GA]^CCGG[CT] 602 GCCGGT DdeI 403 CTCAG C^T[ACGT]AG 550 CTGAG DsaI 167 CCGCGG C^C[GA][CT]GG 439 CCGCGG HgaI 379 GACGC GACGC(5/10) 420 GACGC KspI 167 CCGCGG CCGC^GG 439 CCGCGG MaeIII 217 GTTAC ^GT[ACGT]AC 328 GTCAC PpuMI 290 AGGACCT [GA]G^G[AT]CC[CT] 358 GGGTCCC SacII 167 CCGCGG CCGC^GG 439 CCGCGG SmaI 148 CCCGGG CCC^GGG 355 CCCGGG XmaCI 148 CCCGGG C^CCGGG 355 CCCGGG XmaI 148 CCCGGG C^CCGGG 355 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AluI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI