(Linear) (Six-base) MAPSORT of: KIEE1009.seq Check: 4535 from: 1 to: 660 KIEE1009.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 12 660 Size: 12 648 AleI CACnn'nnGTG Cuts at: 0 343 660 Size: 343 317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 361 660 Size: 361 299 ApaI G_GGCC'C Cuts at: 0 525 660 Size: 525 135 AvaI C'yCGr_G Cuts at: 0 148 355 660 Size: 148 207 305 Fragments arranged by size: 305 207 148 BanII G_rGCy'C Cuts at: 0 356 525 585 660 Size: 356 169 60 75 Fragments arranged by size: 356 169 75 60 BbsI GAAGACnn'nnnn_ Cuts at: 0 56 660 Size: 56 604 BbvCI CC'TCA_GC Cuts at: 0 403 660 Size: 403 257 BlpI GC'TnA_GC Cuts at: 0 550 660 Size: 550 110 Bme1580I G_kGCm'C Cuts at: 0 525 660 Size: 525 135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 307 508 660 Size: 256 51 201 152 Fragments arranged by size: 256 201 152 51 Bpu10I CC'TnA_GC Cuts at: 0 403 660 Size: 403 257 BsaHI Gr'CG_yC Cuts at: 0 421 660 Size: 421 239 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 97 286 452 660 Size: 97 189 166 208 Fragments arranged by size: 208 189 166 97 BseYI C'CCAG_C Cuts at: 0 142 310 468 660 Size: 142 168 158 192 Fragments arranged by size: 192 168 158 142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 660 Size: 586 74 BsiHKAI G_wGCw'C Cuts at: 0 308 660 Size: 308 352 Bsp1286I G_dGCh'C Cuts at: 0 308 356 525 585 660 Size: 308 48 169 60 75 Fragments arranged by size: 308 169 75 60 48 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 125 660 Size: 125 535 BsrBI CCG'CTC Cuts at: 0 372 460 660 Size: 372 88 200 Fragments arranged by size: 372 200 88 BsrFI r'CCGG_y Cuts at: 0 331 602 660 Size: 331 271 58 Fragments arranged by size: 331 271 58 BssHII G'CGCG_C Cuts at: 0 448 660 Size: 448 212 BtgI C'CryG_G Cuts at: 0 167 439 660 Size: 167 272 221 Fragments arranged by size: 272 221 167 EarI CTCTTCn'nnn_ Cuts at: 0 28 660 Size: 28 632 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 170 660 Size: 170 490 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 12 256 307 508 660 Size: 12 244 51 201 152 Fragments arranged by size: 244 201 152 51 12 EcoO109I rG'GnC_Cy Cuts at: 0 247 291 359 660 Size: 247 44 68 301 Fragments arranged by size: 301 247 68 44 HaeII r_GCGC'y Cuts at: 0 12 484 543 557 648 660 Size: 12 472 59 14 91 12 Fragments arranged by size: 472 91 59 14 12 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 343 375 660 Size: 343 32 285 Fragments arranged by size: 343 285 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 59 660 Size: 59 601 MslI CAynn'nnrTG Cuts at: 0 343 660 Size: 343 317 MspA1I CmG'CkG Cuts at: 0 169 441 519 531 570 660 Size: 169 272 78 12 39 90 Fragments arranged by size: 272 169 90 78 39 12 NspI r_CATG'y Cuts at: 0 318 660 Size: 318 342 PfoI T'CCnGG_A Cuts at: 0 374 660 Size: 374 286 PpuMI rG'GwC_Cy Cuts at: 0 291 359 660 Size: 291 68 301 Fragments arranged by size: 301 291 68 PspOMI G'GGCC_C Cuts at: 0 521 660 Size: 521 139 PstI C_TGCA'G Cuts at: 0 518 660 Size: 518 142 SacII CC_GC'GG Cuts at: 0 170 442 660 Size: 170 272 218 Fragments arranged by size: 272 218 170 SanDI GG'GwC_CC Cuts at: 0 359 660 Size: 359 301 SfcI C'TryA_G Cuts at: 0 514 660 Size: 514 146 SmaI CCC'GGG Cuts at: 0 150 357 660 Size: 150 207 303 Fragments arranged by size: 303 207 150 StyI C'CwwG_G Cuts at: 0 557 660 Size: 557 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 156 247 660 Size: 156 91 413 Fragments arranged by size: 413 156 91 Tth111I GACn'n_nGTC Cuts at: 0 257 660 Size: 257 403 XmaI C'CCGG_G Cuts at: 0 148 355 660 Size: 148 207 305 Fragments arranged by size: 305 207 148 Enzymes that do cut: AcuI AleI AlfI ApaI AvaI BanII BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BtgI EarI EciI Eco57MI EcoO109I HaeII Hin4I MmeI MslI MspA1I NspI PfoI PpuMI PspOMI PstI SacII SanDI SfcI SmaI StyI TaqII Tth111I XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XcmI XhoI XmnI ZraI