################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AvrII BbvCI BciVI BfaI BfrI BlnI BlpI Bpu1102I BseAI BsiWI BspDI BspEI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I EcoO65I EcoRI EcoRV FseI HincII HindII HindIII HpaI KpnI MaeI MfeI MluI MroI MunI NcoI NdeI NheI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TfiI XbaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AspI 939 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2627 TGCGCA TGC^GCA BamHI 1991 GGATCC G^GATCC BbeI 283 GGCGCC GGCGC^C BbrPI 2113 CACGTG CAC^GTG BbsI 2424 GAAGAC GAAGAC(2/6) BcgI 1703 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 851 TGATCA T^GATCA BglII 665 AGATCT A^GATCT BpuAI 2424 GAAGAC GAAGAC(2/6) BsaBI 2625 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2974 GAGACC GGTCTC(1/5) BsaWI 915 TCCGGT [AT]^CCGG[AT] BsmBI 239 CGTCTC CGTCTC(1/5) BsmI 732 GAATGC GAATGC(1/-1) Bsp1407I 644 TGTACA T^GTACA BsrFI 1933 GCCGGC [GA]^CCGG[CT] BsrGI 644 TGTACA T^GTACA BssHII 90 GCGCGC G^CGCGC Cfr10I 1933 GCCGGC [GA]^CCGG[CT] DrdI 2680 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2071 CGGCCG C^GGCCG EclXI 2071 CGGCCG C^GGCCG Eco52I 2071 CGGCCG C^GGCCG FbaI 851 TGATCA T^GATCA FspI 2627 TGCGCA TGC^GCA KasI 283 GGCGCC G^GCGCC KspI 2125 CCGCGG CCGC^GG MamI 2625 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 3026 TTAA T^TAA NaeI 1933 GCCGGC GCC^GGC NarI 283 GGCGCC GG^CGCC NgoMIV 1933 GCCGGC G^CCGGC NotI 2070 GCGGCCGC GC^GGCCGC PflFI 939 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2113 CACGTG CAC^GTG PmlI 2113 CACGTG CAC^GTG SacII 2125 CCGCGG CCGC^GG ScaI 1835 AGTACT AGT^ACT SfoI 283 GGCGCC GGC^GCC SspBI 644 TGTACA T^GTACA StuI 1998 AGGCCT AGG^CCT Tru9I 3026 TTAA T^TAA Tth111I 939 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 586 GAATTT [GA]^AATT[CT] 823 AAATTT Alw44I 2554 GTGCAC G^TGCAC 3019 GTGCAC AocI 1803 CCTGAGG CC^T[ACGT]AGG 2877 CCTGAGG Aor51HI 2011 AGCGCT AGC^GCT 2516 AGCGCT ApaLI 2554 GTGCAC G^TGCAC 3019 GTGCAC ApoI 586 GAATTT [GA]^AATT[CT] 823 AAATTT Asp700I 2274 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2424 GAAGACCTTC BanII 64 GGGCTC G[GA]GC[CT]^C 549 GGGCTC BsaAI 1231 TACGTG [CT]AC^GT[GA] 2113 CACGTG BsgI 2448 CTGCAC GTGCAG(16/14) 1792 GTGCAG BspMI 374 ACCTGC ACCTGC(4/8) 442 GCAGGT BsrDI 681 CATTGC GCAATG(2/0) 1596 CATTGC BstAPI 1179 GCATGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2799 GCAGATGCTGC BstXI 527 CCAACGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2334 CCAGTCCTGTGG Bsu36I 1803 CCTGAGG CC^T[ACGT]AGG 2877 CCTGAGG Eco47III 2011 AGCGCT AGC^GCT 2516 AGCGCT Eco81I 1803 CCTGAGG CC^T[ACGT]AGG 2877 CCTGAGG EcoT22I 921 ATGCAT ATGCA^T 2737 ATGCAT NsiI 921 ATGCAT ATGCA^T 2737 ATGCAT PaeR7I 58 CTCGAG C^TCGAG 3178 CTCGAG PstI 385 CTGCAG CTGCA^G 2918 CTGCAG SapI 211 GAAGAGC GCTCTTC(1/4) 834 GCTCTTC SmaI 947 CCCGGG CCC^GGG 1105 CCCGGG XhoI 58 CTCGAG C^TCGAG 3178 CTCGAG XmaCI 947 CCCGGG C^CCGGG 1105 CCCGGG XmaI 947 CCCGGG C^CCGGG 1105 CCCGGG XmnI 2274 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2424 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrBI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII