(Circular) (Six-base) MAPSORT of: pFN21AE6729.seq Check: 2362 from: 1 to: 7960 pFN21AE6729 7960 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2438 2438 Size: 7960 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5770 278 Size: 53 83 186 936 4234 2468 Fragments arranged by size: 4234 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5303 1937 Size: 3366 4594 Acc65I G'GTAC_C Cuts at: 5281 5281 Size: 7960 AclI AA'CG_TT Cuts at: 1563 6087 6460 1563 Size: 4524 373 3063 Fragments arranged by size: 4524 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3236 3388 4118 6023 7407 7675 1632 Size: 1604 152 730 1905 1384 268 1917 Fragments arranged by size: 1917 1905 1604 1384 730 268 152 AfeI AGC'GCT Cuts at: 4018 4523 4018 Size: 505 7455 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7763 AflIII A'CryG_T Cuts at: 1203 2730 3548 4545 6859 7836 1203 Size: 1527 818 997 2314 977 1327 Fragments arranged by size: 2314 1527 1327 997 977 818 AgeI A'CCGG_T Cuts at: 6791 6791 Size: 7960 AhdI GACnn_n'nnGTC Cuts at: 6689 6689 Size: 7960 AleI CACnn'nnGTG Cuts at: 1166 2021 4566 5069 1166 Size: 855 2545 503 4057 Fragments arranged by size: 4057 2545 855 503 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3618 4595 1184 Size: 2434 977 4549 Fragments arranged by size: 4549 2434 977 AlwNI CAG_nnn'CTG Cuts at: 3122 4087 4810 5703 7275 3122 Size: 965 723 893 1572 3807 Fragments arranged by size: 3807 1572 965 893 723 ApaLI G'TGCA_C Cuts at: 4559 5024 6017 7173 4559 Size: 465 993 1156 5346 Fragments arranged by size: 5346 1156 993 465 ApoI r'AATT_y Cuts at: 1493 2591 2828 5269 5495 5647 7756 1493 Size: 1098 237 2441 226 152 2109 1697 Fragments arranged by size: 2441 2109 1697 1098 237 226 152 AseI AT'TA_AT Cuts at: 160 1032 6514 160 Size: 872 5482 1606 Fragments arranged by size: 5482 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7960 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2952 3110 3208 5183 5285 1870 Size: 73 120 889 158 98 1975 102 4545 Fragments arranged by size: 4545 1975 889 158 120 102 98 73 BamHI G'GATC_C Cuts at: 3996 5290 5657 3996 Size: 1294 367 6299 Fragments arranged by size: 6299 1294 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2288 2324 2347 2360 Size: 325 217 627 236 265 36 23 13 Cuts at: 2360 3425 3580 5281 6736 618 Size: 1065 155 1701 1455 1842 Fragments arranged by size: 1842 1701 1455 1065 627 325 265 236 217 155 36 23 13 BanII G_rGCy'C Cuts at: 1391 2073 2558 5279 1391 Size: 682 485 2721 4072 Fragments arranged by size: 4072 2721 682 485 BbeI G_GCGC'C Cuts at: 2292 2292 Size: 7960 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4436 928 Size: 971 2537 4452 Fragments arranged by size: 4452 2537 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3697 3731 6151 6185 1903 Size: 34 50 34 1676 34 2420 34 3678 Fragments arranged by size: 3678 2420 1676 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5852 7062 5852 Size: 1210 6750 BclI T'GATC_A Cuts at: 1548 2856 1548 Size: 1308 6652 BfrBI ATG'CAT Cuts at: 2928 4744 2928 Size: 1816 6144 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3923 4356 4472 Size: 107 122 71 631 605 2251 433 116 Cuts at: 4472 6571 136 Size: 2099 1525 Fragments arranged by size: 2251 2099 1525 631 605 433 122 116 107 71 BglII A'GATC_T Cuts at: 2670 7955 2670 Size: 5285 2675 BlpI GC'TnA_GC Cuts at: 5377 5377 Size: 7960 Bme1580I G_kGCm'C Cuts at: 1790 2327 2363 2444 3497 3583 3676 4488 4563 Size: 537 36 81 1053 86 93 812 75 Cuts at: 4563 5028 6021 7177 1790 Size: 465 993 1156 2573 Fragments arranged by size: 2573 1156 1053 993 812 537 465 93 86 81 75 36 BmgBI CAC'GTC Cuts at: 1318 2733 4546 4953 1318 Size: 1415 1813 407 4325 Fragments arranged by size: 4325 1813 1415 407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2591 3859 5691 Size: 416 53 83 399 24 1174 1268 1832 Cuts at: 5691 6649 442 Size: 958 1753 Fragments arranged by size: 1832 1753 1268 1174 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7960 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3246 3246 Size: 7960 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3116 3179 3548 4064 4550 4636 4910 6620 Size: 1877 63 369 516 486 86 274 1710 Cuts at: 6620 1239 Size: 2579 Fragments arranged by size: 2579 1877 1710 516 486 369 274 86 63 Bpu10I CC'TnA_GC Cuts at: 4013 4230 4013 Size: 217 7743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4142 4736 5225 6085 6950 7248 4142 Size: 594 489 860 865 298 4854 Fragments arranged by size: 4854 865 860 594 489 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4973 6623 882 Size: 617 3474 1650 2219 Fragments arranged by size: 3474 2219 1650 617 BsaAI yAC'GTr Cuts at: 493 1204 3238 4120 5751 493 Size: 711 2034 882 1631 2702 Fragments arranged by size: 2702 2034 1631 882 711 BsaBI GATnn'nnATC Cuts at: 4634 7954 4634 Size: 3320 4640 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2289 3342 5109 5767 Size: 53 83 186 936 756 1053 1767 658 Cuts at: 5767 6149 6784 275 Size: 382 635 1451 Fragments arranged by size: 1767 1451 1053 936 756 658 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2920 6393 6791 7065 7212 7623 2920 Size: 3473 398 274 147 411 3257 Fragments arranged by size: 3473 3257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2448 2478 2589 2619 4784 Size: 30 165 30 1045 30 111 30 2165 Cuts at: 4784 4814 4883 4913 1178 Size: 30 69 30 4225 Fragments arranged by size: 4225 2165 1045 165 111 69 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 2456 3668 3892 4047 4061 4127 1186 Size: 1132 138 1212 224 155 14 66 5019 Fragments arranged by size: 5019 1212 1132 224 155 138 66 14 BseYI C'CCAG_C Cuts at: 2351 2549 2882 2932 3012 3703 4152 4381 4508 Size: 198 333 50 80 691 449 229 127 Cuts at: 4508 4854 4991 7163 7659 2351 Size: 346 137 2172 496 2652 Fragments arranged by size: 2652 2172 691 496 449 346 333 229 198 137 127 80 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3818 4438 1163 Size: 573 2082 620 4685 Fragments arranged by size: 4685 2082 620 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3054 4079 4802 5410 6171 7199 Size: 275 186 1053 1025 723 608 761 1028 Cuts at: 7199 1540 Size: 2301 Fragments arranged by size: 2301 1053 1028 1025 761 723 608 275 186 BsiHKAI G_wGCw'C Cuts at: 2725 3132 4563 5028 6021 6106 7177 2725 Size: 407 1431 465 993 85 1071 3508 Fragments arranged by size: 3508 1431 1071 993 465 407 85 BsmI GAATG_Cn' Cuts at: 2743 5471 5564 2743 Size: 2728 93 5139 Fragments arranged by size: 5139 2728 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2250 2250 Size: 7960 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2327 2363 2444 2558 2725 3132 Size: 399 283 254 36 81 114 167 407 Cuts at: 3132 3497 3583 3676 4488 4563 5028 5279 6021 Size: 365 86 93 812 75 465 251 742 Cuts at: 6021 6106 7177 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 812 742 465 407 399 365 283 254 251 167 114 93 86 85 81 75 36 BspEI T'CCGG_A Cuts at: 7623 7623 Size: 7960 BspHI T'CATG_A Cuts at: 2565 2725 2787 5849 7579 2565 Size: 160 62 3062 1730 2946 Fragments arranged by size: 3062 2946 1730 160 62 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2388 2438 5315 844 Size: 1544 50 2877 3489 Fragments arranged by size: 3489 2877 1544 50 BsrBI CCG'CTC Cuts at: 1394 1823 3324 4777 4840 5209 5847 1394 Size: 429 1501 1453 63 369 638 3507 Fragments arranged by size: 3507 1501 1453 638 429 369 63 BsrDI GCAATG_nn' Cuts at: 65 1248 2685 3600 6455 6629 65 Size: 1183 1437 915 2855 174 1396 Fragments arranged by size: 2855 1437 1396 1183 915 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3938 6604 6791 1700 Size: 110 2128 2666 187 2869 Fragments arranged by size: 2869 2666 2128 187 110 BsrGI T'GTAC_A Cuts at: 96 2649 96 Size: 2553 5407 BssHII G'CGCG_C Cuts at: 1925 2095 1925 Size: 170 7790 BssSI C'ACGA_G Cuts at: 3395 4481 4517 6020 7032 3395 Size: 1086 36 1503 1012 4323 Fragments arranged by size: 4323 1503 1086 1012 36 BstAPI GCAn_nnn'nTGC Cuts at: 794 3190 4810 794 Size: 2396 1620 3944 Fragments arranged by size: 3944 2396 1620 BstBI TT'CG_AA Cuts at: 6764 6764 Size: 7960 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7960 BstXI CCAn_nnnn'nTGG Cuts at: 2539 4346 2539 Size: 1807 6153 BstYI r'GATC_y Cuts at: 1972 2670 3096 3996 4941 5290 5657 6042 6059 Size: 698 426 900 945 349 367 385 17 Cuts at: 6059 7511 7597 7609 7955 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 945 900 698 426 385 367 349 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3809 4883 3809 Size: 1074 6886 BtgI C'CryG_G Cuts at: 513 1065 2019 3643 4130 4374 513 Size: 552 954 1624 487 244 4099 Fragments arranged by size: 4099 1624 954 552 487 244 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2509 3235 3480 3679 3700 3919 Size: 683 56 1283 726 245 199 21 219 Cuts at: 3919 4480 487 Size: 561 3967 Fragments arranged by size: 3967 1283 726 683 561 245 219 199 56 21 BtsI GCAGTG_nn' Cuts at: 3415 4932 5064 5476 6270 6290 3415 Size: 1517 132 412 794 20 5085 Fragments arranged by size: 5085 1517 794 412 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7960 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3573 3608 529 Size: 35 3009 35 4881 Fragments arranged by size: 4881 3009 35 35 DraI TTT'AAA Cuts at: 5264 5264 Size: 7960 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4691 6967 809 Size: 507 3375 2276 1802 Fragments arranged by size: 3375 2276 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 3006 3375 Size: 54 1425 50 275 18 675 501 369 Cuts at: 3375 3640 3924 4076 4302 5233 5407 6296 7925 Size: 265 284 152 226 931 174 889 1629 Cuts at: 7925 8 Size: 43 Fragments arranged by size: 1629 1425 931 889 675 501 369 284 275 265 226 174 152 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4076 5407 1537 Size: 275 2264 1331 4090 Fragments arranged by size: 4090 2264 1331 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2211 2253 2420 2846 5890 1353 Size: 858 42 167 426 3044 3423 Fragments arranged by size: 3423 3044 858 426 167 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2219 6547 6921 7067 387 Size: 1064 138 630 4328 374 146 1280 Fragments arranged by size: 4328 1280 1064 630 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3116 3179 3236 3388 3548 4064 4118 Size: 393 1484 63 57 152 160 516 54 Cuts at: 4118 4550 4636 4910 6023 6620 7407 7675 1239 Size: 432 86 274 1113 597 787 268 1524 Fragments arranged by size: 1524 1484 1113 787 597 516 432 393 274 268 160 152 86 63 57 54 EcoNI CCTnn'n_nnAGG Cuts at: 2282 3161 3593 2282 Size: 879 432 6649 Fragments arranged by size: 6649 879 432 EcoO109I rG'GnC_Cy Cuts at: 2461 2943 3331 3813 4052 4111 4171 4414 4684 Size: 482 388 482 239 59 60 243 270 Cuts at: 4684 4987 5200 2461 Size: 303 213 5221 Fragments arranged by size: 5221 482 482 388 303 270 243 239 213 60 59 EcoRI G'AATT_C Cuts at: 5269 5647 5269 Size: 378 7582 FspI TGC'GCA Cuts at: 4634 6466 4634 Size: 1832 6128 FspAI rTGC'GCAy Cuts at: 4634 4634 Size: 7960 HaeII r_GCGC'y Cuts at: 1678 2292 3153 3558 3857 4020 4525 7107 1678 Size: 614 861 405 299 163 505 2582 2531 Fragments arranged by size: 2582 2531 861 614 505 405 299 163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4784 4816 4859 4891 6601 Size: 32 135 32 3212 32 43 32 1710 Cuts at: 6601 6633 6675 6707 1373 Size: 32 42 32 2626 Fragments arranged by size: 3212 2626 1710 135 43 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5304 5550 669 Size: 988 281 3366 246 3079 Fragments arranged by size: 3366 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7960 HpaI GTT'AAC Cuts at: 5550 5550 Size: 7960 KasI G'GCGC_C Cuts at: 2288 2288 Size: 7960 KpnI G_GTAC'C Cuts at: 5285 5285 Size: 7960 MfeI C'AATT_G Cuts at: 5559 5559 Size: 7960 MluI A'CGCG_T Cuts at: 7836 7836 Size: 7960 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7074 7258 1305 Size: 5769 184 2007 Fragments arranged by size: 5769 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3008 3377 3642 3926 4304 Size: 54 1425 343 1176 369 265 284 378 Cuts at: 4304 10 Size: 3666 Fragments arranged by size: 3666 1425 1176 378 369 343 284 265 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 2366 2372 2513 4544 4566 4577 Size: 648 855 345 6 141 2031 22 11 Cuts at: 4577 4673 4749 5069 5918 6277 6436 518 Size: 96 76 320 849 359 159 2042 Fragments arranged by size: 2042 2031 855 849 648 359 345 320 159 141 96 76 22 11 6 MspA1I CmG'CkG Cuts at: 1606 2549 2699 2984 3119 4084 4132 4798 4858 Size: 943 150 285 135 965 48 666 60 Cuts at: 4858 5376 6053 7201 7446 1606 Size: 518 677 1148 245 2120 Fragments arranged by size: 2120 1148 965 943 677 666 518 285 245 150 135 60 48 NaeI GCC'GGC Cuts at: 3940 3940 Size: 7960 NarI GG'CG_CC Cuts at: 2289 2289 Size: 7960 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7408 NdeI CA'TA_TG Cuts at: 387 387 Size: 7960 NgoMIV G'CCGG_C Cuts at: 3938 3938 Size: 7960 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7960 NotI GC'GGCC_GC Cuts at: 4076 5407 4076 Size: 1331 6629 NsiI A_TGCA'T Cuts at: 2930 4746 2930 Size: 1816 6144 NspI r_CATG'y Cuts at: 1132 1229 2932 4363 4576 5062 5134 5318 6863 Size: 97 1703 1431 213 486 72 184 1545 Cuts at: 6863 1132 Size: 2229 Fragments arranged by size: 2229 1703 1545 1431 486 213 184 97 72 PasI CC'CwG_GG Cuts at: 1348 2284 3516 1348 Size: 936 1232 5792 Fragments arranged by size: 5792 1232 936 PciI A'CATG_T Cuts at: 6859 6859 Size: 7960 PflMI CCAn_nnn'nTGG Cuts at: 1108 2594 4071 4297 1108 Size: 1486 1477 226 4771 Fragments arranged by size: 4771 1486 1477 226 PfoI T'CCnGG_A Cuts at: 3132 3450 5013 3132 Size: 318 1563 6079 Fragments arranged by size: 6079 1563 318 PmeI GTTT'AAAC Cuts at: 5264 5264 Size: 7960 PmlI CAC'GTG Cuts at: 4120 4120 Size: 7960 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6029 6061 7542 7574 6029 Size: 32 1481 32 6415 Fragments arranged by size: 6415 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2943 3331 3813 4111 4414 4684 4987 2943 Size: 388 482 298 303 270 303 5916 Fragments arranged by size: 5916 482 388 303 303 298 270 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7960 PsiI TTA'TAA Cuts at: 5530 5530 Size: 7960 PspXI vC'TCGA_Gb Cuts at: 2063 5183 2063 Size: 3120 4840 PstI C_TGCA'G Cuts at: 830 2394 4927 5312 830 Size: 1564 2533 385 3478 Fragments arranged by size: 3478 2533 1564 385 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7960 PvuII CAG'CTG Cuts at: 2549 2699 2984 3119 4084 4858 2549 Size: 150 285 135 965 774 5651 Fragments arranged by size: 5651 965 774 285 150 135 SacII CC_GC'GG Cuts at: 4133 4133 Size: 7960 SalI G'TCGA_C Cuts at: 1936 5302 1936 Size: 3366 4594 SanDI GG'GwC_CC Cuts at: 4987 4987 Size: 7960 SapI GCTCTTCn'nnn_ Cuts at: 2211 2846 2211 Size: 635 7325 SbfI CC_TGCA'GG Cuts at: 5312 5312 Size: 7960 ScaI AGT'ACT Cuts at: 3842 6208 7920 3842 Size: 2366 1712 3882 Fragments arranged by size: 3882 2366 1712 SfcI C'TryA_G Cuts at: 826 1045 2390 2456 2985 4923 5308 6443 7124 Size: 219 1345 66 529 1938 385 1135 681 Cuts at: 7124 7315 826 Size: 191 1471 Fragments arranged by size: 1938 1471 1345 1135 681 529 385 219 191 66 SfoI GGC'GCC Cuts at: 2290 2290 Size: 7960 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7960 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7960 SmaI CCC'GGG Cuts at: 1872 2954 3112 5287 1872 Size: 1082 158 2175 4545 Fragments arranged by size: 4545 2175 1082 158 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 4157 4715 5183 5204 6064 Size: 197 926 120 2094 558 468 21 860 Cuts at: 6064 6965 7227 820 Size: 901 262 1553 Fragments arranged by size: 2094 1553 926 901 860 558 468 262 197 120 21 SnaBI TAC'GTA Cuts at: 493 5751 493 Size: 5258 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7960 SphI G_CATG'C Cuts at: 1132 2932 4363 4576 5062 5318 1132 Size: 1800 1431 213 486 256 3774 Fragments arranged by size: 3774 1800 1431 486 256 213 SspI AAT'ATT Cuts at: 5 52 5884 5 Size: 47 5832 2081 Fragments arranged by size: 5832 2081 47 StuI AGG'CCT Cuts at: 1517 4005 1517 Size: 2488 5472 StyI C'CwwG_G Cuts at: 513 1065 2160 3606 3967 5399 513 Size: 552 1095 1446 361 1432 3074 Fragments arranged by size: 3074 1446 1432 1095 552 361 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2068 2297 2365 3026 3068 3107 5158 Size: 1255 488 229 68 661 42 39 2051 Cuts at: 5158 5210 5987 6004 6157 6342 6787 325 Size: 52 777 17 153 185 445 1498 Fragments arranged by size: 2051 1498 1255 777 661 488 445 229 185 153 68 52 42 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 3399 3840 Size: 274 80 33 51 1443 672 750 441 Cuts at: 3840 4899 4968 6206 7918 96 Size: 1059 69 1238 1712 138 Fragments arranged by size: 1712 1443 1238 1059 750 672 441 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2589 2621 3128 3160 7920 7952 2589 Size: 32 507 32 4760 32 2597 Fragments arranged by size: 4760 2597 507 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2947 1316 Size: 1631 6329 XbaI T'CTAG_A Cuts at: 5296 5296 Size: 7960 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2536 2653 4160 2536 Size: 117 1507 6336 Fragments arranged by size: 6336 1507 117 XhoI C'TCGA_G Cuts at: 1943 2063 5183 1943 Size: 120 3120 4720 Fragments arranged by size: 4720 3120 120 XmaI C'CCGG_G Cuts at: 1870 2952 3110 5285 1870 Size: 1082 158 2175 4545 Fragments arranged by size: 4545 2175 1082 158 XmnI GAAnn'nnTTC Cuts at: 4283 4433 6089 4283 Size: 150 1656 6154 Fragments arranged by size: 6154 1656 150 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5768 276 Size: 53 83 186 936 4234 2468 Fragments arranged by size: 4234 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI NruI PacI PspOMI PsrI RsrII SacI SexAI SfiI SrfI SwaI