(Linear) (Six-base) MAPSORT of: KIEE6729.seq Check: 9609 from: 1 to: 3255 KIEE6729.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 433 3255 Size: 433 2822 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1231 1383 2113 3255 Size: 1231 152 730 1142 Fragments arranged by size: 1231 1142 730 152 AfeI AGC'GCT Cuts at: 0 2013 2518 3255 Size: 2013 505 737 Fragments arranged by size: 2013 737 505 AflIII A'CryG_T Cuts at: 0 725 1543 2540 3255 Size: 725 818 997 715 Fragments arranged by size: 997 818 725 715 AleI CACnn'nnGTG Cuts at: 0 16 2561 3064 3255 Size: 16 2545 503 191 Fragments arranged by size: 2545 503 191 16 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1613 2590 3255 Size: 1613 977 665 Fragments arranged by size: 1613 977 665 AlwNI CAG_nnn'CTG Cuts at: 0 1117 2082 2805 3255 Size: 1117 965 723 450 Fragments arranged by size: 1117 965 723 450 ApaLI G'TGCA_C Cuts at: 0 2554 3019 3255 Size: 2554 465 236 Fragments arranged by size: 2554 465 236 ApoI r'AATT_y Cuts at: 0 586 823 3255 Size: 586 237 2432 Fragments arranged by size: 2432 586 237 AvaI C'yCGr_G Cuts at: 0 58 947 1105 1203 3178 3255 Size: 58 889 158 98 1975 77 Fragments arranged by size: 1975 889 158 98 77 58 BamHI G'GATC_C Cuts at: 0 1991 3255 Size: 1991 1264 BanI G'GyrC_C Cuts at: 0 18 283 319 342 355 1420 1575 3255 Size: 18 265 36 23 13 1065 155 1680 Fragments arranged by size: 1680 1065 265 155 36 23 18 13 BanII G_rGCy'C Cuts at: 0 68 553 3255 Size: 68 485 2702 Fragments arranged by size: 2702 485 68 BbeI G_GCGC'C Cuts at: 0 287 3255 Size: 287 2968 BbsI GAAGACnn'nnnn_ Cuts at: 0 2431 3255 Size: 2431 824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1692 1726 3255 Size: 1692 34 1529 Fragments arranged by size: 1692 1529 34 BclI T'GATC_A Cuts at: 0 851 3255 Size: 851 2404 BfrBI ATG'CAT Cuts at: 0 923 2739 3255 Size: 923 1816 516 Fragments arranged by size: 1816 923 516 BglI GCCn_nnn'nGGC Cuts at: 0 1918 2351 2467 3255 Size: 1918 433 116 788 Fragments arranged by size: 1918 788 433 116 BglII A'GATC_T Cuts at: 0 665 3255 Size: 665 2590 Bme1580I G_kGCm'C Cuts at: 0 322 358 439 1492 1578 1671 2483 2558 Size: 322 36 81 1053 86 93 812 75 Cuts at: 2558 3023 3255 Size: 465 232 Fragments arranged by size: 1053 812 465 322 232 93 86 81 75 36 BmgBI CAC'GTC Cuts at: 0 728 2541 2948 3255 Size: 728 1813 407 307 Fragments arranged by size: 1813 728 407 307 BmrI ACTGGGnnnn_n' Cuts at: 0 586 1854 3255 Size: 586 1268 1401 Fragments arranged by size: 1401 1268 586 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1241 3255 Size: 1241 2014 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1111 1174 1543 2059 2545 2631 2905 3255 Size: 1111 63 369 516 486 86 274 350 Fragments arranged by size: 1111 516 486 369 350 274 86 63 Bpu10I CC'TnA_GC Cuts at: 0 2008 2225 3255 Size: 2008 217 1030 Fragments arranged by size: 2008 1030 217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2137 2731 3220 3255 Size: 2137 594 489 35 Fragments arranged by size: 2137 594 489 35 BsaI GGTCTCn'nnnn_ Cuts at: 0 2968 3255 Size: 2968 287 BsaAI yAC'GTr Cuts at: 0 1233 2115 3255 Size: 1233 882 1140 Fragments arranged by size: 1233 1140 882 BsaBI GATnn'nnATC Cuts at: 0 2629 3255 Size: 2629 626 BsaHI Gr'CG_yC Cuts at: 0 284 1337 3104 3255 Size: 284 1053 1767 151 Fragments arranged by size: 1767 1053 284 151 BsaWI w'CCGG_w Cuts at: 0 915 3255 Size: 915 2340 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 443 473 584 614 2779 2809 2878 2908 Size: 443 30 111 30 2165 30 69 30 Cuts at: 2908 3255 Size: 347 Fragments arranged by size: 2165 443 347 111 69 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 313 451 1663 1887 2042 2056 2122 3255 Size: 313 138 1212 224 155 14 66 1133 Fragments arranged by size: 1212 1133 313 224 155 138 66 14 BseYI C'CCAG_C Cuts at: 0 346 544 877 927 1007 1698 2147 2376 Size: 346 198 333 50 80 691 449 229 Cuts at: 2376 2503 2849 2986 3255 Size: 127 346 137 269 Fragments arranged by size: 691 449 346 346 333 269 229 198 137 127 80 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1813 2433 3255 Size: 1813 620 822 Fragments arranged by size: 1813 822 620 BsiEI CG_ry'CG Cuts at: 0 1049 2074 2797 3255 Size: 1049 1025 723 458 Fragments arranged by size: 1049 1025 723 458 BsiHKAI G_wGCw'C Cuts at: 0 720 1127 2558 3023 3255 Size: 720 407 1431 465 232 Fragments arranged by size: 1431 720 465 407 232 BsmI GAATG_Cn' Cuts at: 0 738 3255 Size: 738 2517 BsmBI CGTCTCn'nnnn_ Cuts at: 0 245 3255 Size: 245 3010 Bsp1286I G_dGCh'C Cuts at: 0 68 322 358 439 553 720 1127 1492 Size: 68 254 36 81 114 167 407 365 Cuts at: 1492 1578 1671 2483 2558 3023 3255 Size: 86 93 812 75 465 232 Fragments arranged by size: 812 465 407 365 254 232 167 114 93 86 81 75 68 36 BspHI T'CATG_A Cuts at: 0 560 720 782 3255 Size: 560 160 62 2473 Fragments arranged by size: 2473 560 160 62 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 383 433 3255 Size: 383 50 2822 Fragments arranged by size: 2822 383 50 BsrBI CCG'CTC Cuts at: 0 1319 2772 2835 3204 3255 Size: 1319 1453 63 369 51 Fragments arranged by size: 1453 1319 369 63 51 BsrDI GCAATG_nn' Cuts at: 0 680 1595 3255 Size: 680 915 1660 Fragments arranged by size: 1660 915 680 BsrFI r'CCGG_y Cuts at: 0 1933 3255 Size: 1933 1322 BsrGI T'GTAC_A Cuts at: 0 644 3255 Size: 644 2611 BssHII G'CGCG_C Cuts at: 0 90 3255 Size: 90 3165 BssSI C'ACGA_G Cuts at: 0 1390 2476 2512 3255 Size: 1390 1086 36 743 Fragments arranged by size: 1390 1086 743 36 BstAPI GCAn_nnn'nTGC Cuts at: 0 1185 2805 3255 Size: 1185 1620 450 Fragments arranged by size: 1620 1185 450 BstXI CCAn_nnnn'nTGG Cuts at: 0 534 2341 3255 Size: 534 1807 914 Fragments arranged by size: 1807 914 534 BstYI r'GATC_y Cuts at: 0 665 1091 1991 2936 3255 Size: 665 426 900 945 319 Fragments arranged by size: 945 900 665 426 319 Bsu36I CC'TnA_GG Cuts at: 0 1804 2878 3255 Size: 1804 1074 377 Fragments arranged by size: 1804 1074 377 BtgI C'CryG_G Cuts at: 0 14 1638 2125 2369 3255 Size: 14 1624 487 244 886 Fragments arranged by size: 1624 886 487 244 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 504 1230 1475 1674 1695 1914 2475 3255 Size: 504 726 245 199 21 219 561 780 Fragments arranged by size: 780 726 561 504 245 219 199 21 BtsI GCAGTG_nn' Cuts at: 0 1410 2927 3059 3255 Size: 1410 1517 132 196 Fragments arranged by size: 1517 1410 196 132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1568 1603 3255 Size: 1568 35 1652 Fragments arranged by size: 1652 1568 35 DrdI GACnn_nn'nnGTC Cuts at: 0 2686 3255 Size: 2686 569 EaeI y'GGCC_r Cuts at: 0 500 1001 1370 1635 1919 2071 2297 3228 Size: 500 501 369 265 284 152 226 931 Cuts at: 3228 3255 Size: 27 Fragments arranged by size: 931 501 500 369 284 265 226 152 27 EagI C'GGCC_G Cuts at: 0 2071 3255 Size: 2071 1184 EarI CTCTTCn'nnn_ Cuts at: 0 206 248 415 841 3255 Size: 206 42 167 426 2414 Fragments arranged by size: 2414 426 206 167 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 214 3255 Size: 214 3041 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1111 1174 1231 1383 1543 2059 2113 2545 Size: 1111 63 57 152 160 516 54 432 Cuts at: 2545 2631 2905 3255 Size: 86 274 350 Fragments arranged by size: 1111 516 432 350 274 160 152 86 63 57 54 EcoNI CCTnn'n_nnAGG Cuts at: 0 277 1156 1588 3255 Size: 277 879 432 1667 Fragments arranged by size: 1667 879 432 277 EcoO109I rG'GnC_Cy Cuts at: 0 456 938 1326 1808 2047 2106 2166 2409 Size: 456 482 388 482 239 59 60 243 Cuts at: 2409 2679 2982 3195 3255 Size: 270 303 213 60 Fragments arranged by size: 482 482 456 388 303 270 243 239 213 60 60 59 FspI TGC'GCA Cuts at: 0 2629 3255 Size: 2629 626 FspAI rTGC'GCAy Cuts at: 0 2629 3255 Size: 2629 626 HaeII r_GCGC'y Cuts at: 0 287 1148 1553 1852 2015 2520 3255 Size: 287 861 405 299 163 505 735 Fragments arranged by size: 861 735 505 405 299 287 163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2779 2811 2854 2886 3255 Size: 2779 32 43 32 369 Fragments arranged by size: 2779 369 43 32 32 KasI G'GCGC_C Cuts at: 0 283 3255 Size: 283 2972 MscI TGG'CCA Cuts at: 0 1003 1372 1637 1921 2299 3255 Size: 1003 369 265 284 378 956 Fragments arranged by size: 1003 956 378 369 284 265 MslI CAynn'nnrTG Cuts at: 0 16 361 367 508 2539 2561 2572 2668 Size: 16 345 6 141 2031 22 11 96 Cuts at: 2668 2744 3064 3255 Size: 76 320 191 Fragments arranged by size: 2031 345 320 191 141 96 76 22 16 11 6 MspA1I CmG'CkG Cuts at: 0 544 694 979 1114 2079 2127 2793 2853 Size: 544 150 285 135 965 48 666 60 Cuts at: 2853 3255 Size: 402 Fragments arranged by size: 965 666 544 402 285 150 135 60 48 NaeI GCC'GGC Cuts at: 0 1935 3255 Size: 1935 1320 NarI GG'CG_CC Cuts at: 0 284 3255 Size: 284 2971 NgoMIV G'CCGG_C Cuts at: 0 1933 3255 Size: 1933 1322 NotI GC'GGCC_GC Cuts at: 0 2071 3255 Size: 2071 1184 NsiI A_TGCA'T Cuts at: 0 925 2741 3255 Size: 925 1816 514 Fragments arranged by size: 1816 925 514 NspI r_CATG'y Cuts at: 0 927 2358 2571 3057 3129 3255 Size: 927 1431 213 486 72 126 Fragments arranged by size: 1431 927 486 213 126 72 PasI CC'CwG_GG Cuts at: 0 279 1511 3255 Size: 279 1232 1744 Fragments arranged by size: 1744 1232 279 PflMI CCAn_nnn'nTGG Cuts at: 0 589 2066 2292 3255 Size: 589 1477 226 963 Fragments arranged by size: 1477 963 589 226 PfoI T'CCnGG_A Cuts at: 0 1127 1445 3008 3255 Size: 1127 318 1563 247 Fragments arranged by size: 1563 1127 318 247 PmlI CAC'GTG Cuts at: 0 2115 3255 Size: 2115 1140 PpuMI rG'GwC_Cy Cuts at: 0 938 1326 1808 2106 2409 2679 2982 3255 Size: 938 388 482 298 303 270 303 273 Fragments arranged by size: 938 482 388 303 303 298 273 270 PspXI vC'TCGA_Gb Cuts at: 0 58 3178 3255 Size: 58 3120 77 Fragments arranged by size: 3120 77 58 PstI C_TGCA'G Cuts at: 0 389 2922 3255 Size: 389 2533 333 Fragments arranged by size: 2533 389 333 PvuII CAG'CTG Cuts at: 0 544 694 979 1114 2079 2853 3255 Size: 544 150 285 135 965 774 402 Fragments arranged by size: 965 774 544 402 285 150 135 SacII CC_GC'GG Cuts at: 0 2128 3255 Size: 2128 1127 SanDI GG'GwC_CC Cuts at: 0 2982 3255 Size: 2982 273 SapI GCTCTTCn'nnn_ Cuts at: 0 206 841 3255 Size: 206 635 2414 Fragments arranged by size: 2414 635 206 ScaI AGT'ACT Cuts at: 0 1837 3255 Size: 1837 1418 SfcI C'TryA_G Cuts at: 0 385 451 980 2918 3255 Size: 385 66 529 1938 337 Fragments arranged by size: 1938 529 385 337 66 SfoI GGC'GCC Cuts at: 0 285 3255 Size: 285 2970 SmaI CCC'GGG Cuts at: 0 949 1107 3255 Size: 949 158 2148 Fragments arranged by size: 2148 949 158 SmlI C'TyrA_G Cuts at: 0 58 2152 2710 3178 3199 3255 Size: 58 2094 558 468 21 56 Fragments arranged by size: 2094 558 468 58 56 21 SphI G_CATG'C Cuts at: 0 927 2358 2571 3057 3255 Size: 927 1431 213 486 198 Fragments arranged by size: 1431 927 486 213 198 StuI AGG'CCT Cuts at: 0 2000 3255 Size: 2000 1255 StyI C'CwwG_G Cuts at: 0 155 1601 1962 3255 Size: 155 1446 361 1293 Fragments arranged by size: 1446 1293 361 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 63 292 360 1021 1063 1102 3153 3205 Size: 63 229 68 661 42 39 2051 52 Cuts at: 3205 3255 Size: 50 Fragments arranged by size: 2051 661 229 68 63 52 50 42 39 TatI w'GTAC_w Cuts at: 0 644 1394 1835 2894 2963 3255 Size: 644 750 441 1059 69 292 Fragments arranged by size: 1059 750 644 441 292 69 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 584 616 1123 1155 3255 Size: 584 32 507 32 2100 Fragments arranged by size: 2100 584 507 32 32 Tth111I GACn'n_nGTC Cuts at: 0 942 3255 Size: 942 2313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 531 648 2155 3255 Size: 531 117 1507 1100 Fragments arranged by size: 1507 1100 531 117 XhoI C'TCGA_G Cuts at: 0 58 3178 3255 Size: 58 3120 77 Fragments arranged by size: 3120 77 58 XmaI C'CCGG_G Cuts at: 0 947 1105 3255 Size: 947 158 2150 Fragments arranged by size: 2150 947 158 XmnI GAAnn'nnTTC Cuts at: 0 2278 2428 3255 Size: 2278 150 827 Fragments arranged by size: 2278 827 150 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV NotI NsiI NspI PasI PflMI PfoI PmlI PpuMI PspXI PstI PvuII SacII SanDI SapI ScaI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BlpI BmtI BsiWI BspEI BstBI BstEII BstZ17I ClaI DraI DraIII EcoICRI EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NruI PacI PciI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SalI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI ZraI