################################## # NO CUT ENZYMES ( 207 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 119 GTAC GT^AC AvaI 25 CTCGGG C^[CT]CG[GA]G BalI 17 TGGCCA TGG^CCA BanI 178 GGTGCC G^G[CT][GA]CC BmyI 179 GTGCCC G[AGT]GC[ACT]^C BsaI 210 GGTCTC GGTCTC(1/5) BsaJI 164 CCAAGG C^C[ACGT][ACGT]GG BseRI 108 CTCCTC GAGGAG(10/8) BsoBI 25 CTCGGG C^[CT]CG[GA]G Bsp1286I 179 GTGCCC G[AGT]GC[ACT]^C BsrDI 10 GCAATG GCAATG(2/0) BssKI 20 CCAGG ^CC[ACGT]GG BstNI 20 CCAGG CC^[AT]GG BstXI 161 CCACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 45 GCTGGC GC[ACGT]^[ACGT]GC Cfr13I 138 GGCCC G^G[ACGT]CC EaeI 17 TGGCCA [CT]^GGCC[GA] EcoRII 20 CCAGG ^CC[AT]GG EcoT14I 164 CCAAGG C^C[AT][AT]GG HincII 189 GTCAAC GT[CT]^[GA]AC HindII 189 GTCAAC GT[CT]^[GA]AC MaeIII 104 GTAAC ^GT[ACGT]AC MluNI 17 TGGCCA TGG^CCA MnlI 110 CCTC CCTC(7/6) MscI 17 TGGCCA TGG^CCA MslI 162 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 20 CCAGG CC^[AT]GG PstI 184 CTGCAG CTGCA^G RsaI 119 GTAC GT^AC Sau96I 138 GGCCC G^G[ACGT]CC ScaI 118 AGTACT AGT^ACT ScrFI 20 CCAGG CC^[ACGT]GG SfaNI 228 GCATC GCATC(5/9) SfcI 184 CTGCAG C^T[GA][CT]AG StyI 164 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BsiYI 20 CCAGGCTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 125 CCAACTCTGGG BslI 20 CCAGGCTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 125 CCAACTCTGGG BsmAI 200 GTCTC GTCTC(1/5) 211 GTCTC EarI 202 CTCTTC CTCTTC(1/4) 221 CTCTTC HaeIII 18 GGCC GG^CC 138 GGCC Ksp632I 202 CTCTTC CTCTTC(1/4) 221 CTCTTC MboII 203 TCTTC GAAGA(8/7) 222 TCTTC NlaIV 138 GGCCCC GG[ACGT]^[ACGT]CC 178 GGTGCC TspRI 50 CACTG CA[GC]TG[ACGT][ACGT]^ 78 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BbvI BsrI Fnu4HI ItaI MwoI TseI XcmI