################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tsp509I TspRI XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 163 GACGTC GACGT^C AciI 48 CCGC CCGC(-3/-1) AcyI 163 GACGTC G[GA]^CG[CT]C AfaI 285 GTAC GT^AC AlwI 254 GATCC GGATC(4/5) AlwNI 267 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 145 GTGCTC G[AT]GC[AT]^C AspI 163 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 45 GGACC G^G[AT]CC BbsI 160 GAAGAC GAAGAC(2/6) BcnI 42 CCCGG CC^[GC]GG BpmI 264 CTCCAG CTGGAG(16/14) BpuAI 160 GAAGAC GAAGAC(2/6) BsaBI 248 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 163 GACGTC G[GA]^CG[CT]C BsiHKAI 145 GTGCTC G[AT]GC[AT]^C BsrI 266 CCAGT ACTGG(1/-1) CfoI 276 GCGC GCG^C CpoI 44 CGGACCG CG^G[AT]CCG DpnII 254 GATC ^GATC EaeI 2 TGGCCG [CT]^GGCC[GA] HaeII 275 GGCGCT [GA]GCGC^[CT] HapII 43 CCGG C^CGG HgaI 153 GCGTC GACGC(5/10) HhaI 276 GCGC GCG^C Hin1I 163 GACGTC G[GA]^CG[CT]C HinP1I 276 GCGC G^CGC HinfI 262 GACTC G^A[ACGT]TC HpaII 43 CCGG C^CGG HphI 32 TCACC GGTGA(8/7) MaeII 164 ACGT A^CGT MamI 248 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 254 GATC ^GATC MboII 160 GAAGA GAAGA(8/7) MseI 109 TTAA T^TAA MspI 43 CCGG C^CGG NciI 42 CCCGG CC^[GC]GG NdeII 254 GATC ^GATC NspI 314 ACATGC [GA]CATG^[CT] PaeR7I 322 CTCGAG C^TCGAG PflFI 163 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 266 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 262 GACTC GAGTC(4/5) RsaI 285 GTAC GT^AC RsrII 44 CGGACCG CG^G[AT]CCG Sau3AI 254 GATC ^GATC SmlI 322 CTCGAG C^T[CT][GA]AG StuI 178 AGGCCT AGG^CCT Tru9I 109 TTAA T^TAA Tsp45I 31 GTCAC ^GT[GC]AC Tth111I 163 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 266 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 322 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 230 AGCT AG^CT 326 AGCT AvaI 19 CTCGGG C^[CT]CG[GA]G 322 CTCGAG BmyI 9 GTGCCC G[AGT]GC[ACT]^C 145 GTGCTC BsaJI 59 CCTGGG C^C[ACGT][ACGT]GG 171 CCTGGG BsoBI 19 CTCGGG C^[CT]CG[GA]G 322 CTCGAG Bsp1286I 9 GTGCCC G[AGT]GC[ACT]^C 145 GTGCTC Cac8I 93 GCACGC GC[ACGT]^[ACGT]GC 209 GCCTGC DdeI 258 CTCAG C^T[ACGT]AG 279 CTTAG MwoI 93 GCACGCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 318 GCTTCTCGAGC NlaIII 222 CATG CATG^ 315 CATG TaqI 116 TCGA T^CGA 323 TCGA TthHB8I 116 TCGA T^CGA 323 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### BbvI BsiYI BslI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII ItaI MaeIII MnlI MvaI Sau96I ScrFI TseI