(Circular) (Six-base) MAPSORT of: pFN21AA1560.seq Check: 6823 from: 1 to: 7189 pFN21AA1560 7189 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4999 278 Size: 53 83 186 936 3463 2468 Fragments arranged by size: 3463 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 4532 1937 Size: 1086 1509 4594 Fragments arranged by size: 4594 1509 1086 Acc65I G'GTAC_C Cuts at: 4510 4510 Size: 7189 AclI AA'CG_TT Cuts at: 1563 5316 5689 1563 Size: 3753 373 3063 Fragments arranged by size: 3753 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2119 2751 2779 4142 4213 5252 6636 6904 Size: 487 632 28 1363 71 1039 1384 268 Cuts at: 6904 1632 Size: 1917 Fragments arranged by size: 1917 1384 1363 1039 632 487 268 71 28 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6992 AflIII A'CryG_T Cuts at: 1203 2954 3342 6088 7065 1203 Size: 1751 388 2746 977 1327 Fragments arranged by size: 2746 1751 1327 977 388 AgeI A'CCGG_T Cuts at: 3253 6020 3253 Size: 2767 4422 AhdI GACnn_n'nnGTC Cuts at: 5918 5918 Size: 7189 AleI CACnn'nnGTG Cuts at: 1166 2094 2387 1166 Size: 928 293 5968 Fragments arranged by size: 5968 928 293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7189 AlwNI CAG_nnn'CTG Cuts at: 3622 4250 4932 6504 3622 Size: 628 682 1572 4307 Fragments arranged by size: 4307 1572 682 628 ApaLI G'TGCA_C Cuts at: 5246 6402 5246 Size: 1156 6033 ApoI r'AATT_y Cuts at: 1493 2234 2619 2665 3586 4498 4724 4876 6985 Size: 741 385 46 921 912 226 152 2109 Cuts at: 6985 1493 Size: 1697 Fragments arranged by size: 2109 1697 921 912 741 385 226 152 46 AseI AT'TA_AT Cuts at: 160 1032 3351 5743 160 Size: 872 2319 2392 1606 Fragments arranged by size: 2392 2319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7189 AvaI C'yCGr_G Cuts at: 1870 1943 2985 4514 1870 Size: 73 1042 1529 4545 Fragments arranged by size: 4545 1529 1042 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2233 2266 4238 4271 2233 Size: 33 1972 33 5151 Fragments arranged by size: 5151 1972 33 33 BamHI G'GATC_C Cuts at: 4519 4886 4519 Size: 367 6822 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3893 4510 5965 618 Size: 325 217 627 2106 617 1455 1842 Fragments arranged by size: 2106 1842 1455 627 617 325 217 BanII G_rGCy'C Cuts at: 1391 4508 1391 Size: 3117 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3531 3694 4089 928 Size: 971 1632 163 395 4028 Fragments arranged by size: 4028 1632 971 395 163 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5380 5414 1903 Size: 34 3443 34 3678 Fragments arranged by size: 3678 3443 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2261 5081 6291 2261 Size: 2820 1210 3159 Fragments arranged by size: 3159 2820 1210 BclI T'GATC_A Cuts at: 1548 2559 3831 1548 Size: 1011 1272 4906 Fragments arranged by size: 4906 1272 1011 BfrBI ATG'CAT Cuts at: 3607 3607 Size: 7189 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5800 136 Size: 107 122 71 631 605 4128 1525 Fragments arranged by size: 4128 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2138 2937 7184 2138 Size: 799 4247 2143 Fragments arranged by size: 4247 2143 799 BlpI GC'TnA_GC Cuts at: 3411 4020 4606 3411 Size: 609 586 5994 Fragments arranged by size: 5994 609 586 Bme1580I G_kGCm'C Cuts at: 1790 5250 6406 1790 Size: 3460 1156 2573 Fragments arranged by size: 3460 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3345 1318 Size: 2027 5162 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2241 2580 3119 Size: 416 53 83 399 24 824 339 539 Cuts at: 3119 3309 3809 4920 5878 442 Size: 190 500 1111 958 1753 Fragments arranged by size: 1753 1111 958 824 539 500 416 399 339 190 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3435 1056 Size: 2379 4810 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2906 2906 Size: 7189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3296 4211 5849 1239 Size: 2057 915 1638 2579 Fragments arranged by size: 2579 2057 1638 915 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2657 5314 6179 6477 2657 Size: 2657 865 298 3369 Fragments arranged by size: 3369 2657 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 5852 882 Size: 617 2880 1473 2219 Fragments arranged by size: 2880 2219 1473 617 BsaAI yAC'GTr Cuts at: 493 1204 4980 493 Size: 711 3776 2702 Fragments arranged by size: 3776 2702 711 BsaBI GATnn'nnATC Cuts at: 7183 7183 Size: 7189 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2326 4996 5378 6013 Size: 53 83 186 936 793 2670 382 635 Cuts at: 6013 275 Size: 1451 Fragments arranged by size: 2670 1451 936 793 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3253 3957 5622 6020 6294 6441 6852 3253 Size: 704 1665 398 274 147 411 3590 Fragments arranged by size: 3590 1665 704 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3269 3299 3866 3896 4158 Size: 30 165 30 1866 30 567 30 262 Cuts at: 4158 4188 1178 Size: 30 4179 Fragments arranged by size: 4179 1866 567 262 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3470 1186 Size: 2284 4905 BseYI C'CCAG_C Cuts at: 2226 2472 3622 3680 6392 6888 2226 Size: 246 1150 58 2712 496 2527 Fragments arranged by size: 2712 2527 1150 496 246 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2001 2197 1163 Size: 573 265 196 6155 Fragments arranged by size: 6155 573 265 196 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4639 5400 6428 1540 Size: 275 186 2638 761 1028 2301 Fragments arranged by size: 2638 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2986 3987 4146 5250 5335 6406 2986 Size: 1001 159 1104 85 1071 3769 Fragments arranged by size: 3769 1104 1071 1001 159 85 BsmI GAATG_Cn' Cuts at: 3966 4351 4700 4793 3966 Size: 385 349 93 6362 Fragments arranged by size: 6362 385 349 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2654 2654 Size: 7189 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2986 3987 4146 4508 5250 5335 6406 Size: 399 1196 1001 159 362 742 85 1071 Cuts at: 6406 1391 Size: 2174 Fragments arranged by size: 2174 1196 1071 1001 742 399 362 159 85 BspEI T'CCGG_A Cuts at: 6852 6852 Size: 7189 BspHI T'CATG_A Cuts at: 5078 6808 5078 Size: 1730 5459 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4544 844 Size: 3700 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5076 1394 Size: 429 3253 3507 Fragments arranged by size: 3507 3253 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2562 2760 3730 5684 5858 65 Size: 1183 1314 198 970 1954 174 1396 Fragments arranged by size: 1954 1396 1314 1183 970 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2551 3253 5833 6020 1700 Size: 110 741 702 2580 187 2869 Fragments arranged by size: 2869 2580 741 702 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7189 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7189 BssSI C'ACGA_G Cuts at: 3558 5249 6261 3558 Size: 1691 1012 4486 Fragments arranged by size: 4486 1691 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3850 4267 4313 794 Size: 3056 417 46 3670 Fragments arranged by size: 3670 3056 417 46 BstBI TT'CG_AA Cuts at: 3705 5993 3705 Size: 2288 4901 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7189 BstXI CCAn_nnnn'nTGG Cuts at: 2816 2816 Size: 7189 BstYI r'GATC_y Cuts at: 1972 2138 2696 2937 4519 4886 5271 5288 6740 Size: 166 558 241 1582 367 385 17 1452 Cuts at: 6740 6826 6838 7184 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1582 1452 558 385 367 346 241 166 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2308 2893 513 Size: 552 939 304 585 4809 Fragments arranged by size: 4809 939 585 552 304 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6450 Fragments arranged by size: 6450 683 56 BtsI GCAGTG_nn' Cuts at: 3426 3902 4034 4705 5499 5519 3426 Size: 476 132 671 794 20 5096 Fragments arranged by size: 5096 794 671 476 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7189 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3445 3480 4213 4248 529 Size: 35 2881 35 733 35 3470 Fragments arranged by size: 3470 2881 733 35 35 35 DraI TTT'AAA Cuts at: 2151 4493 2151 Size: 2342 4847 DraIII CAC_nnn'GTG Cuts at: 3466 3466 Size: 7189 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3804 6196 809 Size: 507 2488 2392 1802 Fragments arranged by size: 2488 2392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3861 4636 5525 Size: 54 1425 50 275 18 2031 775 889 Cuts at: 5525 7154 8 Size: 1629 43 Fragments arranged by size: 2031 1629 1425 889 775 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4636 1537 Size: 275 2824 4090 Fragments arranged by size: 4090 2824 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2738 5119 1353 Size: 1385 2381 3423 Fragments arranged by size: 3423 2381 1385 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5776 6150 6296 387 Size: 1064 138 4187 374 146 1280 Fragments arranged by size: 4187 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2751 2779 3296 4142 4211 4213 Size: 393 487 632 28 517 846 69 2 Cuts at: 4213 5252 5849 6636 6904 1239 Size: 1039 597 787 268 1524 Fragments arranged by size: 1524 1039 846 787 632 597 517 487 393 268 69 28 2 EcoO109I rG'GnC_Cy Cuts at: 3806 3806 Size: 7189 EcoRI G'AATT_C Cuts at: 4498 4876 4498 Size: 378 6811 EcoRV GAT'ATC Cuts at: 4003 4003 Size: 7189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2651 2651 Size: 7189 FspI TGC'GCA Cuts at: 5695 5695 Size: 7189 HaeII r_GCGC'y Cuts at: 1678 6336 1678 Size: 4658 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 2826 2858 3005 Size: 32 135 32 543 32 679 32 147 Cuts at: 3005 3037 3388 3420 5830 5862 5904 5936 1373 Size: 32 351 32 2410 32 42 32 2626 Fragments arranged by size: 2626 2410 679 543 351 147 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2494 2633 4533 4779 669 Size: 988 281 556 139 1900 246 3079 Fragments arranged by size: 3079 1900 988 556 281 246 139 HindIII A'AGCT_T Cuts at: 748 2813 4040 748 Size: 2065 1227 3897 Fragments arranged by size: 3897 2065 1227 HpaI GTT'AAC Cuts at: 2494 4779 2494 Size: 2285 4904 KpnI G_GTAC'C Cuts at: 4514 4514 Size: 7189 MfeI C'AATT_G Cuts at: 2430 4788 2430 Size: 2358 4831 MluI A'CGCG_T Cuts at: 7065 7065 Size: 7189 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2156 2173 3040 6303 6487 1305 Size: 851 17 867 3263 184 2007 Fragments arranged by size: 3263 2007 867 851 184 17 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5367 Fragments arranged by size: 5367 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2094 2387 3096 5147 5506 5665 518 Size: 648 928 293 709 2051 359 159 2042 Fragments arranged by size: 2051 2042 928 709 648 359 293 159 MspA1I CmG'CkG Cuts at: 1606 2310 3619 3847 4605 5282 6430 6675 1606 Size: 704 1309 228 758 677 1148 245 2120 Fragments arranged by size: 2120 1309 1148 758 704 677 245 228 NcoI C'CATG_G Cuts at: 513 1065 2004 2893 513 Size: 552 939 889 4809 Fragments arranged by size: 4809 939 889 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7189 NheI G'CTAG_C Cuts at: 1052 3431 1052 Size: 2379 4810 NotI GC'GGCC_GC Cuts at: 4636 4636 Size: 7189 NsiI A_TGCA'T Cuts at: 3609 3609 Size: 7189 NspI r_CATG'y Cuts at: 1132 1229 2377 3611 4547 6092 1132 Size: 97 1148 1234 936 1545 2229 Fragments arranged by size: 2229 1545 1234 1148 936 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7189 PciI A'CATG_T Cuts at: 6088 6088 Size: 7189 PflMI CCAn_nnn'nTGG Cuts at: 1108 2192 1108 Size: 1084 6105 PfoI T'CCnGG_A Cuts at: 2943 2943 Size: 7189 PmeI GTTT'AAAC Cuts at: 4493 4493 Size: 7189 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5258 5290 6771 6803 5258 Size: 32 1481 32 5644 Fragments arranged by size: 5644 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3806 3806 Size: 7189 PshAI GACnn'nnGTC Cuts at: 1613 2998 1613 Size: 1385 5804 PsiI TTA'TAA Cuts at: 4759 4759 Size: 7189 PstI C_TGCA'G Cuts at: 830 4541 830 Size: 3711 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7189 PvuII CAG'CTG Cuts at: 3619 3847 3619 Size: 228 6961 SacII CC_GC'GG Cuts at: 2311 2311 Size: 7189 SalI G'TCGA_C Cuts at: 1936 4531 1936 Size: 2595 4594 SanDI GG'GwC_CC Cuts at: 3806 3806 Size: 7189 SbfI CC_TGCA'GG Cuts at: 4541 4541 Size: 7189 ScaI AGT'ACT Cuts at: 5437 7149 5437 Size: 1712 5477 SfcI C'TryA_G Cuts at: 826 1045 2878 3484 3718 4198 4537 5672 6353 Size: 219 1833 606 234 480 339 1135 681 Cuts at: 6353 6544 826 Size: 191 1471 Fragments arranged by size: 1833 1471 1135 681 606 480 339 234 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7189 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7189 SmaI CCC'GGG Cuts at: 1872 4516 1872 Size: 2644 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2636 5293 6194 6456 820 Size: 197 926 693 2657 901 262 1553 Fragments arranged by size: 2657 1553 926 901 693 262 197 SnaBI TAC'GTA Cuts at: 493 4980 493 Size: 4487 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7189 SphI G_CATG'C Cuts at: 1132 3611 4547 1132 Size: 2479 936 3774 Fragments arranged by size: 3774 2479 936 SspI AAT'ATT Cuts at: 5 52 3230 5113 5 Size: 47 3178 1883 2081 Fragments arranged by size: 3178 2081 1883 47 StuI AGG'CCT Cuts at: 1517 4196 1517 Size: 2679 4510 StyI C'CwwG_G Cuts at: 513 1065 2004 2026 2893 4137 4177 4628 513 Size: 552 939 22 867 1244 40 451 3074 Fragments arranged by size: 3074 1244 939 867 552 451 40 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2562 2566 3659 5216 5233 5386 5571 Size: 1255 982 4 1093 1557 17 153 185 Cuts at: 5571 6016 325 Size: 445 1498 Fragments arranged by size: 1557 1498 1255 1093 982 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2419 5435 7147 Size: 274 80 33 51 1443 442 3016 1712 Cuts at: 7147 96 Size: 138 Fragments arranged by size: 3016 1712 1443 442 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7149 7181 7149 Size: 32 7157 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7189 XbaI T'CTAG_A Cuts at: 4459 4525 4459 Size: 66 7123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3463 3619 3463 Size: 156 7033 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7189 XmaI C'CCGG_G Cuts at: 1870 4514 1870 Size: 2644 4545 XmnI GAAnn'nnTTC Cuts at: 2913 4467 5318 2913 Size: 1554 851 4784 Fragments arranged by size: 4784 1554 851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4997 276 Size: 53 83 186 936 3463 2468 Fragments arranged by size: 3463 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI